Person:
Navarro García, Federico

First Name

Federico

Last Name

Navarro García

URI

https://hdl.handle.net/20.500.14352/77657

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Farmacia

Department

Microbiología y Parasitología

Area

Microbiología

Identifiers

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Now showing 1 - 10 of 11

Project number: 369
Dinamizando el aula: implementación de Wooclap en la docencia de Microbiología e Inmunología, y su utilidad en aprendizaje significativo
(2024) Román González, Elvira; Pla Alonso, Jesús; Navarro García, Federico; Prieto Prieto, Antonio Daniel; García Pastor, Lucía; Blesa Esteban, Alba Mercedes
Limitations and Challenges in the Stability of Cysteamine Eye Drop Compounded Formulations
(Pharmaceuticals, 2022) Martín Sabroso, Cristina; Alonso González, Mario; Fernández Carballido, Ana María; Aparicio Blanco, Juan; Córdoba Díaz, Damián; Navarro García, Federico; Córdoba Díaz, Manuel; Torres Suárez, Ana Isabel
Accumulation of cystine crystals in the cornea of patients suffering from cystinosis is considered pathognomonic and can lead to severe ocular complications. Cysteamine eye drop compounded formulations, commonly prepared by hospital pharmacy services, are meant to diminish the build-up of corneal cystine crystals. The objective of this work was to analyze whether the shelf life proposed for six formulations prepared following different protocols used in hospital pharmacies is adequate to guarantee the quality and efficacy of cysteamine eye drops. The long-term and in-use stabilities of these preparations were studied using different parameters: content of cysteamine and its main degradation product cystamine; appearance, color and odor; pH and viscosity; and microbiological analysis. The results obtained show that degradation of cysteamine was between 20% and 50% after one month of storage in the long-term stability study and between 35% and 60% in the in-use study. These data confirm that cysteamine is a very unstable molecule in aqueous solution, the presence of oxygen being the main degradation factor. Saturation with nitrogen gas of the solutions offers a means of reducing cysteamine degradation. Overall, all the formulae studied presented high instability at the end of their shelf life, suggesting that their clinical efficacy might be dramatically compromised.
Project number: 21
De la “lección magistral” al Aprendizaje Basado en Proyectos (ABP) "personalizado" a las características del alumnado de Ciencias de la Salud
(2020) Rodríguez Escudero, María Isabel; Díez Orejas, Rosalía María; Navarro García, Federico; Pitarch Velasco, Aída; Pérez Chica, María Almudena
En el presente Proyecto llevamos a cabo la implantación del Aprendizaje Basado en Proyectos (ABP) en el aula universitaria utilizando la asignatura de Microbiología del Grado en Farmacia, en Ciencia y Tecnología de los Alimentos, y en Óptica y Optometría. En cada uno de dichos Grados se comparó esta metodología con la tradicional clase magistral y además se adaptó el ABP en los tres Grados, según la idiosincrasia del alumnado en cada uno de ellos, constituyendo lo que llamamos ABP “personalizado”.
When structure means conservation: Effect of aggregate structure in controlling microbial responses to rewetting events
(Soil Biology & Biochemistry, 2011) Navarro García, Federico; Casermeiro Martínez, Miguel Ángel; Schimel, Joshua P.
Rewetting events after a drought produce a pulse of soil respiration (the “Birch Effect”) that leads to a loss of carbon from soil, especially in Mediterranean ecosystems. Two main hypotheses have developed to explain the Birch effect: the “metabolic explanation”, based on the rapid consumption of intracellular osmolytes previously accumulated to survive to dry conditions, and the “physical explanation”, based on the consumption of carbon made accessible by physical destruction of internal structures of the soil. Here, we compared the respiration response of intact and crushed 9e4 mm aggregates from a California grassland soil under two different rewetting schemes: (1) successive short dry/wet events and (2) increased drought periods followed by a single rewetting. In intact aggregates, both microbial biomass and respiration rates were relatively stable through both experimental treatments. In crushed aggregates, through multiple short dry/wet cycles, both respiration rate and microbial biomass increased, while as drought length increased, biomass was unaffected but the magnitude of the following rewetting pulse increased. A mechanism that explains both these results is that crushing aggregates exposes occluded particular material that must be degraded into an immediately bioavailable form for microbes to take it up and metabolize it. Nitrification was generally higher in intact than crushed aggregates, suggesting the importance of physical association between nitrifiers and resources in regulating overall soil nitrification. This work suggests that physical processes are most important in driving respiration pulses through multiple rewetting cycles and that the physical association of organisms, substrates, and mineral particles are critical in controlling the functioning of the “microbial landscape”.
Project number: 265
SWI@UCM 2.0: Consolidación de Small World Initiative: descubrimiento y uso racional de antibióticos mediante aprendizaje-servicio en la Comunidad de Madrid
(2018) Jiménez Cid, Víctor; Rodríguez Escudero, María Isabel; Díez Orejas, Rosalía María; Molina Martín, María; Rodríguez Fernández, Carmina; Navarro García, Federico; Arroyo Nombela, Francisco Javier; Román González, Elvira; Martín Brieva, Humberto; Fernández-Acero Bascones, Teresa; Sanz Santamaría, Ana Belén; Díaz Del Toro, Silvia; Calvo De Pablo, Pilar; Patiño Álvarez, Aurora Belén; González Zorn, Bruno; Suárez Rodríguez, Mónica; Goyache Goñi, Joaquín; Escudero García-Calderón, José Antonio; Prieto Prieto, Antonio Daniel; Ugarte Ruiz, María; Gil Serna, Jessica
Durante el curso anterior un equipo de la UCM en el marco de un proyecto previo INNOVA-Docencia UCM instauró en España de manera pionera la iniciativa de aprendizaje activo Small World Initiative, de origen norteamericano. Los objetivos de esta iniciativa son (1) Crear cultura científica y acercar la investigación biomédica a niveles educativos en los que los estudiantes tienen aún capacidad de decisión sobre su futura orientación formativa con el fin de fomentar la vocación en I+D; y (2) Promover la concienciación social sobre el uso racional de los antibióticos y la amenaza de la resistencia bacteriana a estos fármacos. En el entorno español se propuso implementar esta estrategia mediante Aprendizaje-Servicio. En esta segunda edición (SWI@UCM 2.0) se ha trabajado en la consolidación, expansión y mejora del proyecto, con énfasis en la integración de los diversos niveles educativos que integran el proyecto (universitario y preuniversitario) y la divulgación científica.
Project number: 40
SWI@UCM: Implantación en España de la Small World Initiative (descubrimiento de antibióticos por “crowdsourcing”) mediante una estrategia de aprendizaje-servicio
(2017) Jiménez Cid, Víctor; González Zorn, Bruno; Valderrama Conde, María José; Calvo De Pablo, Pilar; Navarro García, Federico; Molina Martín, María; Pla Alonso, Jesús; Fernández-Acero Bascones, Teresa; Díez Orejas, Rosalía María; Rodríguez Fernández, Carmina; Román González, Elvira; Martín Brieva, Humberto; Sanz Santamaría, Ana Belén; Prieto Prieto, Antonio Daniel; Arregui García-Roves, Lucía; Vázquez Estévez, María Covadonga Inmaculada; Patiño Álvarez, Aurora Belén; Silóniz Jiménez, María Isabel De; Gil Serna, Jessica; Suárez Rodríguez, Mónica; Juan Ferré, Lucía De; Thomas López, Daniel; Escudero García-Calderón, José Antonio
Small World Initiative (SWI) es un proyecto internacional cuyos objetivos son: (1) acercar la cultura científica y la investigación biomédica a niveles educativos en los que los estudiantes tienen aún capacidad de decisión sobre su futura orientación formativa con el fin de fomentar la vocación en I+D; y (2) divulgar una de las líneas prioritarias marcadas por la Organización Mundial de la Salud (OMS), que es la concienciación social sobre el uso racional de los antibióticos y la amenaza de la resistencia bacteriana. Para lograr estos objetivos SWI desarrolla un proyecto real de investigación dirigido al descubrimiento de nuevos antibióticos mediante una estrategia de “crowdsourcing”, de modo que la comunidad participa de forma activa en las tareas de investigación. En esta experiencia piloto de implantación en España de esta iniciativa, SWI@UCM hemos optado por una estrategia basada en aprendizaje-servicio integrando dos niveles educativos, de modo que los estudiantes universitarios, como parte de su formación, son responsables de la gestión y organización de laboratorios de microbiología en institutos de educación secundaria y bachillerato, dirigiendo a los jóvenes científicos en estudio de la diversidad microbiana en los suelos para el descubrimiento de microorganismos productores de antibióticos potencialmente nuevos.
Educating in antimicrobial resistance awareness: adaptation of the Small World Initiative program to service-learning.
(FEMS Microbiology Letters, 2018) Valderrama Conde, María José; González Zorn, Bruno; Calvo De Pablo, Pilar; Díez Orejas, Rosalía María; Fernández-Acero Bascones, Teresa; Gil Serna, Jessica; Juan Ferré, Lucía De; Martín Brieva, Humberto; Molina Martín, María; Navarro García, Federico; Patiño Álvarez, Aurora Belén; Pla Alonso, Jesús; Prieto, Daniel; Rodríguez Fernández, Carmina; Román González, Elvira; Sanz Santamaría, Ana Belén; Silóniz Jiménez, María Isabel De; Suárez Rodríguez, Mónica; Vázquez Estévez, María Covadonga Inmaculada; Jiménez Cid, Víctor
The Small World Initiative (SWI) and Tiny Earth are a consolidated and successful education programs rooted in the USA that tackle the antibiotic crisis by a crowdsourcing strategy. Based on active learning, it challenges young students to discover novel bioactive-producing microorganisms from environmental soil samples. Besides its pedagogical efficiency to impart microbiology content in academic curricula, SWI promotes vocations in research and development in Experimental Sciences and, at the same time, disseminates the antibiotic awareness guidelines of the World Health Organization. We have adapted the SWI program to the Spanish academic environment by a pioneering hierarchic strategy based on service-learning that involves two education levels (higher education and high school) with different degrees of responsibility. Throughout the academic year, 23 SWI teams, each consisting of 3-7 undergraduate students led by one faculty member, coordinated off-campus programs in 22 local high schools, involving 597 high school students as researchers. Post-survey-based evaluation of the program reveals a satisfactory achievement of goals: acquiring scientific abilities and general or personal competences by university students, as well as promoting academic decisions to inspire vocations for science- and technology-oriented degrees in younger students, and successfully communicating scientific culture in antimicrobial resistance to a young stratum of society.
The tale of microencapsulated rifampicin: is it useful for the treatment of periprosthetic joint infection?
(International Orthopaedics, 2022) López Torres, Irene Isabel; Vaquero Martín, Francisco Javier; Torres Suárez, Ana Isabel; Navarro García, Federico; Fraguas Sánchez, Ana Isabel; León Román, Víctor Estuardo; Sanz Ruiz, Pablo
Purpose Microencapsulation techniques have allowed the addition of rifampicin to bone cement, but its in vivo efficacy has not been proven. The aim of our study is to determine the superiority of cement containing gentamicin and rifampicin microcapsules in the treatment of PJI versus cement exclusively containing gentamicin. Methods An S. aureus PJI was induced in 15 NZW rabbits. A week after inoculation, the first stage of replacement was carried out, and the animals were divided into two groups: group R received a spacer containing gentamicin and rifampicin microcapsules, and group C received a spacer containing gentamicin. Intra-articular release curve of rifampicin and infection and toxicity markers were monitored for four weeks post-operatively, when microbiological analysis was performed. Results The microbiological cultures showed a significantly lower growth of S. aureus in soft tissue (2.3·104 vs 0; p = 0.01) and bone (5.7·102 vs 0; p = 0.03) in the group with rifampicin microcapsules. No differences were found in systemic toxicity markers. Rifampicin release from the cement spacer showed higher concentrations than the staphylococcal MIC throughout the analysis. Conclusion The in vivo analyses demonstrated the superiority of cement containing gentamicin and rifampicin microcapsules versus the isolated use of gentamicin in the treatment of PJI in the rabbit model without serious side effects due to the systemic absorption of rifampicin. Given the increasing incidence of staphylococci-related PJI, the development of new strategies for intra-articular administration of rifampicin for its treatment has a high clinical impact.
Non-canonical Activities of Hog1 Control Sensitivity of Candida albicans to Killer Toxins From Debaryomyces hansenii
(Frontiers in cellular and infection microbiology, 2018) Morales Menchén, Ana; Navarro García, Federico; Guirao Abad, José P.; Román González, Elvira; Prieto Prieto, Antonio Daniel; Coman, Ioana V; Pla Alonso, Jesús; Alonso Monge, Rebeca María Del Mar
Certain yeasts secrete peptides known as killer toxins or mycocins with a deleterious effect on sensitive yeasts or filamentous fungi, a common phenomenon in environmental species. In a recent work, different () strains isolated from a wide variety of cheeses were identified as producing killer toxins active against and . We have analyzed the killer activity of these toxins in mutants defective in MAPK signaling pathways and found that the lack of the MAPK Hog1 (but not Cek1 or Mkc1) renders cells hypersensitive to mycocins while mutants lacking other upstream elements of the pathway behave as the wild type strain. Point mutations in the phosphorylation site (T174A-176F) or in the kinase domain (K52R) of gene showed that both activities were relevant for the survival of to killer toxins. Moreover, Hog1 phosphorylation was also required to sense and adapt to osmotic and oxidative stress while the kinase activity was somehow dispensable. Although the addition of supernatant from the killer toxin- producing 242 strain (-242) induced a slight intracellular increase in Reactive Oxygen Species (ROS), overexpression of cytosolic catalase did not protect against this mycocin. This supernatant induced an increase in intracellular glycerol concentration suggesting that this toxin triggers an osmotic stress. We also provide evidence of a correlation between sensitivity to -242 killer toxin and resistance to Congo red, suggesting cell wall specific alterations in sensitive strains.
Project number: 90
Smartphones y aprendizaje en el entorno del espacio europeo de educación superior
(2015) Navarro García, Federico; Pla Alonso, Jesús; Alonso Monge, Rebeca María Del Mar; Román González, Elvira
Se ha evaluado la utilización de una aplicación gratuita disponible en internet para teléfonos inteligentes (Socrative) con los objetivos de a) motivación y mejora del aprendizaje del alumnado, y b)obtención de retroalimentación para mejorar la docencia que impartimos en la asignatura Microbiología del tercer curso del Grado en Farmacia. Para ello se han realizado preguntas durante el transcurso de la sesión docente que el alumnado debía responder mediante la utilización de esta aplicación. De esta manera, las contestaciones eran anónimas lo que estimulaba a la participación y posterior discusión. Al terminar la sesión docente se les sometía a un test para comprobar si los conceptos explicados habían sido comprendidos y asimilados. Posteriormente se les facilitaba los resultados individualmente para que comprobaran su evolución. En caso de identificar conceptos que en sucesivos test no eran contestados apropiadamente, se procedía a una nueva explicación. Esta metodología nos ha permitido extraer diversas conclusiones: 1)El uso de dispositivos móviles es una metodología eficaz para el aprendizaje, el fomento de la participación del alumnado y el ahorro de recursos económicos en nuestra universidad. 2)El uso de esta estrategia es valorado positivamente por el alumnado. 3)Sería deseable el desarrollo e implementación de una aplicación propia por parte de la UCM que permita la identificación, seguridad y conectividad, así como un marco normativo que dé validez a las evaluaciones realizadas con las aplicaciones móviles.