Person: Millán Pastor, María Pilar
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First Name
María Pilar
Last Name
Millán Pastor
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Fisiología
Area
Fisiología
Identifiers
20 results
Search Results
Now showing 1 - 10 of 20
Item Follicular, Oocyte and Embryo Features Related to Metabolic Status in Primiparous Lactating does Fed with High-Fibre Rearing Diets(Reproduction in Domestic Animals, 2010) Arias Álvarez, María; García García, Rosa María; Rebollar, P.G.; Nicodemus, Nuria; Millán Pastor, María Pilar; Revuelta Rueda, Luis; Lorenzo González, Pedro LuisFertility of primiparous lactating does in the early postpartum (pp) period is very low mainly due to pronounced deficient energy intake, influencing oocyte and embryo developmental competence. The hypothesis used in this work was that high-lignin fibre diet supplied during the rearing period could increase feed intake and, consequently, improve the reproductive physiology and metabolic status of primiparous does in the early pp period. Diets with high-lignin [HL: 15.8% dry matter (DM)] or standard-lignin content (SL: 4.9% DM) were supplied until parturition time. No diet effects in serum oestradiol, progesterone concentrations and follicle categories were found in the histological study. Metaphase II rate of in vitro-matured oocytes was significantly higher in the SL vs the HL group (p < 0.001). Cytoplasmically degenerated oocytes (in terms of abnormal distribution of cortical granules) and follicular atresia rate were significantly lower in the SL group than in the HL group (p < 0.05 and p < 0.005 respectively). In addition, HL-fed does showed lower number of viable embryos and higher rate of retarded in vivo-recovered embryos compared with the SL group (p < 0.05). Neither in vitro embryo development of viable embryos nor conception rate was significantly different between groups. Feed intake increased during the first pregnancy in the HL group (p < 0.05), but not during early lactation. Serum protein, non-esterified fatty acid and leptin concentrations, as well as estimated body composition were similar in does fed with both diets. In conclusion, the enhancement of reproductive management by using highly lignified products in rearing diets does not seem to report physiological reproductive benefits affecting oocyte maturation rate and embryo viability in primiparous lactating does.Item Long‐Term Exposure to Isoflavones Alters the Hormonal Steroid Homeostasis‐Impairing Reproductive Function in Adult Male Wistar Rats(Nutrients, 2023) Cáceres Ramos, Sara Cristina; Crespo, Belen; Alonso Diez, Angela; Andrés Gamazo, Paloma Jimena De; Millán Pastor, María Pilar; Silván Granado, Gema; Illera Del Portal, Josefina María; Illera Del Portal, Juan CarlosThe consumption of isoflavones is gaining popularity worldwide due to their beneficial effects on health. However, isoflavones are considered to be endocrine disruptors and cause deleterious effects on hormone‐sensitive organs, especially in males. Therefore, this study aimed to deter‐ mine if a continuous and prolonged exposure to isoflavones in adult males altered the endocrine axis effect of testicular function. For this purpose, seventy‐five adult male rats were administered with low and high mixtures of isoflavones (genistein and daidzein) for 5 months. The determination of steroid hormones (progesterone, androstenedione, dehydroepiandrosterone, testosterone, dihydrotestosterone, 17β‐estradiol, and estrone sulphate) was carried out in serum and testicular homogenate samples. Sperm quality parameters and testicular histology were also determined. The results revealed that low and high doses of isoflavones promote a hormonal imbalance in androgen and estrogen production, resulting in a decrease in circulating and testicular androgen levels and an increase in estrogen levels. These results are associated with a reduction in the sperm quality parameters and a reduction in the testicular weight, both in the diameter of the seminiferous tubules and the height of the germinal epithelium. Altogether, these results suggest that a continuous ex‐ posure to isoflavones in adult male rats causes a hormonal imbalance in the testes that disrupts the endocrine axis, causing defects in testicular function.Item Physiological effects on rabbit sperm and reproductive response to recombinant rabbit beta nerve growth factor administered by intravaginal route in rabbit does(Theriogenology, 2020) Sánchez Rodríguez, Ana; Arias Álvarez, María; Millán Pastor, María Pilar; Lorenzo González, Pedro Luis; García García, Rosa María; García Rebollar, PilarBeta nerve growth factor (β-NGF) is present in the seminal plasma of some species, including rabbits, acting as an ovulation-inducing factor in camelids. Traditionally, GnRH analogues are used to induce ovulation by intramuscular route when artificial insemination (AI) is performed in rabbit does. A specific rabbit recombinant β-NGF (rrβ-NGF) produced in our laboratory was tested as an alternative method to conventional treatment with GnRH analogues to induce ovulation. In the present work, different concentrations (0, 20, 100 ng/mL and 1, 20 and 100 μg/mL) of rrβ-NGF were added to diluted semen to assess its effect on sperm traits (viability and motility parameters). rrβ-NG was used also, incorporated to the AI dose, to evaluate ovulation response (LH and progesterone plasma concentrations, ovulation rate (OR) and embryo implantation at Day 7) after intravaginal administration. A negative control group stimulated with an empty catheter, and a positive control group inseminated and intramuscularly treated as usual with GnRH were also set up. Results showed that seminal quality was influenced by rrβ-NGF depending on the concentration added, being the highest concentrations tested deleterious for semen. Whereas the highest OR was found in the positive control group (100%), concentrations of 20 ng/mL, 1 μg/mL and 20 μg/mL of rrβ-NGF triggered intermediate OR (30, 60 and 42.9%, respectively), and 100 ng/mL and 100 μg/mL had the lowest OR (20 and 14.3%, respectively). Although LH peak was not observed in the first 2 h after AI in the ovulated females from rrβ-NGF groups, plasma progesterone significantly increased at Day 7, except in those females treated with 20 and 100 μg/mL. Also, 98.4% of ovulated females were pregnant on Day 7. In conclusion, rrβ-NGF added to diluted semen affects seminal quality and provokes ovulation, the development of functional CL and conception by intravaginal route in rabbit does, depending on the concentration added.- Project number: 380
Item El Perro en Investigación Animal (I). Enseñanzas en manejo para incrementar el bienestar animal(2015) Millán Pastor, María Pilar Item Improvements in the conception rate, milk composition and embryo quality of rabbit does after dietary enrichment with n-3 polyunsaturated fatty acids(Animal, 2018) Rodríguez, M.; García García, Rosa María; Arias Álvarez, María; Millán Pastor, María Pilar; Febrel, N.; Formoso-Rafferty Castilla, Nora; López-Tello, J.; Lorenzo González, Pedro Luis; Rebollar, P.G.This work attempts to confirm the effect of an enriched diet with n-3 polyunsaturated fatty acids (PUFA) trying to mitigate the reproductive performances issues such as low conception rate of primiparous rabbits. A total of 127 does were fed ad libitum throughout their two first cycles with two diets with different fat sources: mixed fat in the control and salmon oil in the enriched one, with 3.19 g/100 g (n=63 does) and 28.77 g/100 g (n=64 does) of n-3 of the total fatty acid, respectively. Feed intake was similar between groups (P>0.05). Plasma progesterone concentration was higher in the enriched females than in control ones at 7 (30.9±2.18 v. 23.9±2.30 ng/ml, respectively; P=0.029) and 14 (38.7±2.18 v. 28.2±2.30 ng/ml, respectively; P=0.001) days of first gestation. Considering both cycles, reproductive parameters of mothers (fertility, duration of gestation and prolificacy) and litter parameters (weight at parturition and weaning, mortality and average daily gain (ADG) of kits during lactation) were similar in both groups. However, individual measurements of neonates of enriched group improved 5.87%, 7.10% and 18.01% (P<0.05) in terms of crown-rump length, biparietal and thoracic diameters, respectively, compared to control ones at first parturition. It is noteworthy that at the second insemination, critical point in rabbit, fertility rate of enriched group did not decline as sharply as in the control group (89.7% v. 76.6%, respectively; P=0.067), although ADG and littler weight were slightly lower at the second lactation after PUFA enrichment (P<0.05). Total PUFA and unsaturated index of milk of enriched does group were significantly elevated than in control one (33.3±0.02 v. 23.2±0.02 g/100 g and 1.20±0.00 v. 0.86±0.00, respectively; P<0.05). Finally, plasma progesterone, ovulation rate, fertility and embryo development at 3.5 days after the artificial insemination were similar between diets (P>0.05), but embryo apoptosis rate was higher in control group than in enriched one (31.1±4.56% v. 17.1±3.87%, respectively; P<0.05). In conclusion, dietary PUFA enrichment from the rearing and throughout two productive cycles improved plasma progesterone during pregnancy, fertility, milk fatty acid profile and neonates development of primiparous supporting the beneficial effect of n-3 PUFA supplementation in rabbit does.Item Gestation Food Restriction and Refeeding Compensate Maternal Energy Status and Alleviate Metabolic Consequences in Juvenile Offspring in a Rabbit Model(Nutrients, 2021) García García, Rosa María; Arias Álvarez, María; Millán Pastor, María Pilar; Rodríguez, María; Sánchez Rodríguez, Ana; Lorenzo González, Pedro Luis; Rebollar, Pilar G.Nutritional status during gestation can influence mother and offspring metabolism. Undernutrition in pregnancy affects women in both western and developing countries, and it is associated with a high prevalence of chronic diseases in later life. The present work was conducted in the rabbit model, as a longitudinal study, to examine the effect of food restriction during early and mid-gestation, and re-feeding ad libitum until the end of pregnancy on metabolic status and body reserves of mother and, its association with development and metabolism of fetuses and female offspring to the juvenile stage. Little changes in live body weight (LBW), compensatory feed intake, similar body reserves, and metabolism were observed in dams. Placenta biometry and efficiency were slightly affected, but fetal BW and phenotype were not modified. However, hyperinsulinemia, insulin resistance, and hypertriglyceridemia were demonstrated in pre-term fetuses. In the juvenile period, these changes were not evidenced, and a similar pattern of growth and serum metabolic parameters in offspring of food-restricted mothers were found, except in serum aminotransferases levels, which increased. These were associated with higher liver fibrosis. Maternal food restriction in the early and mid-pregnancy followed by re-feeding in our rabbit model established a compensatory energy status in dams and alleviated potential long-term consequences in growth and metabolism in the offspring, even if fetal metabolism was altered.Item Estudio de la acción de diferentes inmunosupresores (azatioprina, ciclosporina y prednisona) sobre el eje adreno-gonadal y funcionalidad hepática y renal en conejos NZW(2003) Millán Pastor, María Pilar; Illera del Portal, Juan CarlosPara estudiar las relaciones Sistema Inmune, Eje adreno-gonadal y Funcionalidad orgánica, se administraron 3 fármacos inmunosupresores a 40 conejos NZW. Los parámetros séricos estudiados fueron: IgG (EIA Sandwich); corticosterona, androstenodiona, testosterona y estradiol (EIA Competición); Bioquímicos (Hitachi 747). Los tres fármacos produjeron inmunosupresión al disminuir las IgGs. Ciclosporina y Azatioprina estimularon la función adrenal al provocar hiperadrenocorticismo, mientras que la Prednisona produjo hipoadrenocorticismo. Estas alteraciones pueden ser directas o mediadas por las interleucinas del Sistema Inmune (IL-6 e IL-1). La función gonadal fue inhibida por la ciclosporina, por un efecto de retrofuncionalidad negativa de androstenodiona y corticosterona sobre el hipotálamo. La Prednisona alteró significativamente la funcionalidad hepática (ASAT, ALAT, Proteínas T, colesterol y triglicéridos) y renal (Urea y creatinina). Como conclusiones se indican que, el conejo NZW sirve como biomodelo para estudios de la acción de inmunosupresores sobre las funciones orgánicas. La Azatioprina provocó la menor disfunción, sin embargo, los 3 fármacos inmunosupresores ejercen un efecto en la secreción adrenal y/o gonadal, poniendo de manifiesto la intercomunicación entre Sistema Inmune-Sistema Endocrino.- Project number: 234
Item Nuevas herramientas docentes en el espacio universitario multidisciplinar.(2020) Lobato Rincón, Luís Lucio; Illera Del Portal, Josefina María; López Suárez, María Mercedes; Millán Pastor, María Pilar; Pérez Bascuas, Mercedes; Corres Illera, María; Gómez Moreno, María Luisa; Cuevas Lanchares, Juan CarlosNuestro proyecto se fundamenta en la evaluación de 3 competencias que se consideran claves en la enseñanza Universitaria. Se ha seguido como paradigma el planteamiento de Morin (2001), quien señalaba que una de las tendencias esenciales de la educación del futuro, del presente diríamos ya hoy, será el examen y el estudio de la complejidad humana (homo complexus). El objetivo de nuestro Proyecto ya iniciado es desarrollar un análisis de tres competencias consideradas como claves en el ámbito educativo: i. La expresión oral y escrita: saber escribir y expresarse ii. La capacidad para organizar la información: análisis y síntesis y iii. La capacidad para buscar información y utilizar bibliografía. Estas competencias se han aplicado a través de la Estrategia en Pequeños Grupos (EPG) en seis Grados de diferentes Facultades de la UCM: Veterinaria, Ciencias de la Información, Psicología y Ciencias Políticas y Sociología a lo largo de tres cursos académicos el 2017/ 2018, 2018/2019 y parte del curso 2019-2020. Item Distocias Fetales Caninas. Videos 1 y 2. Herramientas multimedia del proyecto de innovación educativa nº 58, 2022-2023.(2023) Mayenco Aguirre, Ana María; Cerdeira Lozano, Joaquín Vicente; Gallo Arranz, Claudia del; Galindo López, Laura; García Fernández, Paloma María; Hernández Hermosa, María José; Martínez-Alesón García, María Paloma; Mayenco Marcos, Sergio; Millán Pastor, María Pilar; Privado Muñoz, Ana María; Vázquez Castellanos, LauraItem Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits(PLoSONE, 2019) Sánchez Rodríguez, Ana; Abad, Paloma; Arias Álvarez, María; Millán Pastor, María Pilar; García Rebollar, Pilar; Bautista Santa Cruz, José Manuel; Lorenzo González, Pedro Luis; García García, Rosa MaríaIn some induced-ovulating species, beta nerve growth factor (β-NGF) has important roles in ovulation, though data for rabbits are still inconclusive. In this study we first synthesized functional recombinant β-NGF from rabbit tissue (rrβ-NGF) to address the following objectives: 1) to compare rabbit β-NGF amino acid sequence with those of other induced- or spontaneous-ovulating species; 2) to assess the effects of rrβ-NGF on rabbit sperm viability and motility, and 3) to examine the in vivo ovulation inducing effect of rrβ-NGF added to the seminal dose in rabbit does. The NGF gene in rabbit prostate tissue was sequenced by Rapid Amplification of cDNA Ends and annotated in GenBank (KX528686). Recombinant rβ-NGF was produced in CHO cells and purified by affinity chromatography. Once confirmed by Western blotting and mass spectrometry (MALDI-TOF) that the amino acid sequence of the recombinant protein corresponded to β-NGF, its functionality was validated in PC12 cells in a successful dose-response study over 8 days. The amino acid sequence of prostate rabbit NGF differed to that of other species mainly in its receptor binding sites. In all the spontaneous ovulating species examined, compared with rabbit, alanine and proline residues, which interact with the high-affinity receptor, were replaced by a serine. In rabbits, asparagine and methionine were substituted by lysine at the low-affinity receptor binding site. In time- and dose-response experiments, the in vitro addition of rrβ-NGF to the ejaculate did not affect sperm viability whereas sperm motility parameters were enhanced by the addition of 1 μg/mL of the neuropeptide. Addition of this same concentration of rrβ-NGF to the seminal dose administered via the intravaginal route in does induced ovulation with a delayed LH peak, leading to a plasma progesterone increase, gestation and delivery. Our findings suggest that rrβ-NGF could be a useful option for biotechnological and reproduction assisted techniques in rabbits but further studies are needed.