Person: Gómez-Lucía Duato, María Esperanza
Loading...
First Name
María Esperanza
Last Name
Gómez-Lucía Duato
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
19 results
Search Results
Now showing 1 - 10 of 19
Item An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus(Applied Microbiology and Biotechnology, 2024) Cea Callejo, Pablo; Arca Lafuente, Sonia; Gómez-Lucía Duato, María Esperanza; Domenech Gómez, Ana María; Biarnés, Mar; Blanco, Ángela; Benítez Rico, Laura; Madrid González, RicardoAirborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming.Item Sintesis de enteroxinas estafilococicas en los alimentos de tipo de elevado consumo(2015) Gómez-Lucía Duato, María EsperanzaItem Avian Leukosis: Will We Be Able to Get Rid of It?(Animals, 2023) Fandiño, Sergio; Gómez-Lucía Duato, María Esperanza; Benítez Rico, Laura; Domenech Gómez, Ana MaríaAvian leukosis viruses (ALVs) have been virtually eradicated from commercial poultry. However, some niches remain as pockets from which this group of viruses may reemerge and induce economic losses. Such is the case of fancy, hobby, backyard chickens and indigenous or native breeds, which are not as strictly inspected as commercial poultry and which have been found to harbor ALVs. In addition, the genome of both poultry and of several gamebird species contain endogenous retroviral sequences. Circumstances that support keeping up surveillance include the detection of several ALV natural recombinants between exogenous and endogenous ALV-related sequences which, combined with the well-known ability of retroviruses to mutate, facilitate the emergence of escape mutants. The subgroup most prevalent nowadays, ALV-J, has emerged as a multi-recombinant which uses a different receptor from the previously known subgroups, greatly increasing its cell tropism and pathogenicity and making it more transmissible. In this review we describe the ALVs, their different subgroups and which receptor they use to infect the cell, their routes of transmission and their presence in different bird collectivities, and the immune response against them. We analyze the different systems to control them, from vaccination to the progress made editing the bird genome to generate mutated ALV receptors or selecting certain haplotypes.Item Evolution of specific antibodies and proviral DNA in milk of small ruminants infected by small ruminant lentivirus(Viruses, 2013) Barquero, Nuria; Gómez-Lucía Duato, María Esperanza; Arjona, Alvaro; Toural, Cristina; Heras, Alfonso las; Domenech Gómez, Ana María; Fernández-Garayzábal Fernández, José FranciscoThe diagnosis of Small Ruminant Lentivirus (SRLV) is based on clinical signs, pathological lesions and laboratory testing. No standard reference test for the diagnosis of maedi visna has been validated up to the present, and it is puzzling that tests which detect antibodies against the virus and tests which detect the proviral genome may render opposite results. The aim of this study was to evaluate the presence in milk throughout a lactation period of specific antibodies by ELISA and of SRLV proviral DNA by a PCR of the highly conserved pol region. A six-month study was conducted with the milk of 28 ewes and 31 goats intensively reared. The percentage of animals with antibodies against SRLV increased throughout the study period. Seroprevalence in sheep was 28% at the beginning of the study and by the end it had increased up to 52.4%. In goats, initial seroprevalence of 5.6% increased to 16%. The percentage of PCR positive ewes was stable throughout the study period. Of the positive sheep, 21.4% were PCR-positive before antibodies could be detected and most of them became PCR-negative shortly after the first detection of antibodies. This might suggest that antibodies have a neutralizing effect. In addition, an equal percentage of sheep were always PCR-negative but either became ELISA-positive or was always ELISA-positive, which might support this hypothesis. On the other hand, the PCR results in goats did not follow any pattern and oscillated between 35.3% and 55.6% depending on the month. Most goats positive by PCR failed to develop antibodies in the 6 months tested. We may conclude that the infection and the antibody response to it follow a different trend in sheep and goats.Item A novel group of avian astroviruses from Neotropical passerine birds broaden the diversity and host range of Astroviridae(Scientific Reports, 2019) Fernández-Correa, Izaskun; Truchado Martín, Daniel Alejandro; Gómez-Lucía Duato, María Esperanza; Domenech Gómez, Ana María; Pérez Tris, Javier; Schmidt-Chanasit, Jonas; Cadar, Daniel; Benítez Rico, LauraMetagenomics is helping to expand the known diversity of viruses, especially of those with poorly studied hosts in remote areas. The Neotropical region harbors a considerable diversity of avian species that may play a role as both host and short-distance vectors of unknown viruses. Viral metagenomics of cloacal swabs from 50 Neotropical birds collected in French Guiana revealed the presence of four complete astrovirus genomes. They constitute an early diverging novel monophyletic clade within the Avastrovirus phylogeny, representing a putative new astrovirus species (provisionally designated as Avastrovirus 5) according to the International Committee on Taxonomy of Viruses (ICTV) classifcation criteria. Their genomic organization shares some characteristics with Avastrovirus but also with Mamastrovirus. The pan-astrovirus RT-PCR analysis of the cloacal samples of 406 wild Neotropical birds showed a community-level prevalence of 4.9% (5.1% in passerines, the highest described so far in this order of birds). By screening birds of a remote region, we expanded the known host range of astroviruses to the avian families Cardinalidae, Conopophagidae, Furnariidae, Thamnophilidae, Turdidae and Tyrannidae. Our results provide important frst insights into the unexplored viral communities, the ecology, epidemiology and features of host-pathogen interactions that shape the evolution of avastroviruses in a remote Neotropical rainforest.Item Clinical and hematological follow-Up of long-term oral therapy with type-I interferon in cats naturally infected with feline Leukemia virus or felineiImmunodeficiency Virus(Animals, 2020) Gómez-Lucía Duato, María Esperanza; Collado Alcalá, Victorio Manuel; Miró Corrales, Guadalupe; Martín Iniesta, Sonsoles; Benítez Rico, Laura; Domenech Gómez, Ana MaríaFeline leukemia virus (FeLV) and feline immunodeficiency virus (FIV), two of the most important pathogens of cats, produce chronic systemic diseases with progressive death of cells involved in the immune response, ultimately leading to death. Immunostimulants is one of the few alternatives to the symptomatic treatment. In this study, 27 naturally FeLV-infected (FeLV+) and 31 naturally FIV-infected (FIV+) cats were administered orally by their owners 60 IU/day of recombinant human interferon alpha (rHuIFN-α) for four months in alternate weeks. Clinical status was evaluated and blood samples collected at four different visits or months (M): pretreatment (M0), mid-treatment (M2), end of treatment (M4), and 4–8 months after end of treatment (M10). Most cats ostensibly improved their clinical status, and many became asymptomatic. rHuIFN-α treatment improved the anemic processes observed at M0 (at least in cats with mild or moderate anemia) and leukocyte counts, including a more favorable CD4+/CD8+ ratio. An increase in the serum gammaglobulin concentration was seen in 80% of the cats. Despite observing an obvious favorable progress in the clinical, biopathological, and CD4+/CD8+ values during treatment, almost invariably all the parameters analyzed worsened after treatment discontinuation (M10), which suggests that the interferon-α protocol should be either extended or include additional cycles for a long-lasting benefit in FeLV+ and FIV+ cats.Item Microbial Matryoshka: Addressing the Relationship between Pathogenic Flagellated Protozoans and Their RNA Viral Endosymbionts (Family Totiviridae).(Veterinary Sciences, 2024) Ibáñez Escribano, Alexandra; Gómez Muñoz, María Teresa; Mateo Barrientos, Marta; Fonseca Berzal, Cristina Rosa; Gómez-Lucía Duato, María Esperanza; García-Pérez, Raquel; Alunda Rodríguez, José María; Carrión Herrero, Francisco JavierGiardiosis, trichomonosis, leishmaniosis, and trypanosomosis are parasitic diseases caused by flagellated protozoa that have a major global health impact, and their control is a priority action line in the agenda of the current One Health Program. The pathogens causing these diseases can establish an endosymbiotic relationship with RNA viruses of the Totiviridae family that can alter the course of the final infection in a mammal. To easily understand the sequence of interactions that occur between the agents involved, from a structural point of view, we can imagine a “matryoshka”-type infection model, wherein the virus represents the smallest matryoshka infecting the flagellated protozoan, which represents the medium matryoshka infecting the mammal, the largest matryoshka. In this manuscript, we will review the available information on the complications generated, such as the aggravation of pathogenesis or treatment failures, because of the established association between these flagellated pathogens and their respective endosymbiont viruses. Accurate diagnosis is required to detect these situations of endosymbiont co-infection and to be able to apply tailor-made treatments that target both the flagellated parasite and the virus that hides inside it. Taken together, these approaches will allow us to achieve and optimize appropriate sanitary control strategies.Item Inmunotrivial: un juego de autoevaluación para el aprendizaje de la inmunología(V Jornada Campus Virtual UCM: buenas prácticas e indicios de calidad, 2009) Blanco Gutiérrez, María Del Mar; Cutuli Simón, María Teresa; Domenech Gómez, Ana María; Domínguez Bernal, Gustavo Ramón; Gibello Prieto, Alicia; Gómez-Lucía Duato, María EsperanzaHoy en día la Inmunología es un instrumento fundamental de la medicina moderna, ya que posibilita la comprensión de los mecanismos de acción de los agentes patógenos y de la respuesta de defensa del organismo frente a ellos. Por tanto, el conocimiento de la Inmunología es vital para los profesionales de distintos ámbitos relacionados con Ciencias de la Salud: médicos, odontólogos, farmacéuticos, biólogos, veterinarios. Por otra parte, el proceso de adaptación curricular al EEES implica una tendencia al aprendizaje de forma más autónoma y al empleo de las nuevas tecnologías a nuestro alcance. Por estos motivos, y con la experiencia que nos proporcionan nuestras actividades tanto de investigación como docentes en Inmunología, así como la experiencia adquirida en la elaboración de nuevos materiales docentes, como el «Manual de Inmunología Veterinaria» (2007, Pearson/Prentice Hall), y el CD «Microbiología Veterinaria. Laboratorio Virtual» (2007, UCM/Editorial Complutense), nos hemos planteado un nuevo reto: el diseño de material de autoevaluación en Inmunología. Se trata de una aplicación informática con formato de juego, en el que el estudiante pone a prueba sus conocimientos en Inmunología de una manera divertida, estimulando el autoaprendizaje y la autoevaluación.Item Comparative metagenomics of Palearctic and Neotropical avian cloacal viromes reveal geographic bias in virus discovery(Microorganisms, 2020) Truchado Martín, Daniel Alejandro; Llanos Garrido, Alejandro; Oropesa Olmedo, David A.; Cerrada, Belén; Cea, Pablo; Moens, Michaël André Jean; Gómez-Lucía Duato, María Esperanza; Domenech Gómez, Ana María; Milá, Borja; Pérez Tris, Javier; Cadar, Daniel; Benítez Rico, LauraOur understanding about viruses carried by wild animals is still scarce. The viral diversity of wildlife may be best described with discovery-driven approaches to the study of viral diversity that broaden research efforts towards non-canonical hosts and remote geographic regions. Birds have been key organisms in the transmission of viruses causing important diseases, and wild birds are threatened by viral spillovers associated with human activities. However, our knowledge of the avian virome may be biased towards poultry and highly pathogenic diseases. We describe and compare the fecal virome of two passerine-dominated bird assemblages sampled in a remote Neotropical rainforest in French Guiana (Nouragues Natural Reserve) and a Mediterranean forest in central Spain (La Herrería). We used metagenomic data to quantify the degree of functional and genetic novelty of viruses recovered by examining if the similarity of the contigs we obtained to reference sequences differed between both locations. In general, contigs from Nouragues were significantly less similar to viruses in databases than contigs from La Herrería using Blastn but not for Blastx, suggesting that pristine regions harbor a yet unknown viral diversity with genetically more singular viruses than more studied areas. Additionally, we describe putative novel viruses of the families Picornaviridae, Reoviridae and Hepeviridae. These results highlight the importance of wild animals and remote regions as sources of novel viruses that substantially broaden the current knowledge of the global diversity of viruses.Item Emerging and Novel Viruses in Passerine Birds(Microorganisms, 2023) Williams, Richard Alexander John; Sánchez-Llatas, Christian ; Domenech Gómez, Ana María; Madrid González, Ricardo; Fandiño, Sergio ; Cea-Callejo, Pablo; Gómez-Lucía Duato, María Esperanza; Benítez Rico, LauraThere is growing interest in emerging viruses that can cause serious or lethal disease in humans and animals. The proliferation of cloacal virome studies, mainly focused on poultry and other domestic birds, reveals a wide variety of viruses, although their pathogenic significance is currently uncertain. Analysis of viruses detected in wild birds is complex and often biased towards waterfowl because of the obvious interest in avian influenza or other zoonotic viruses. Less is known about the viruses present in the order Passeriformes, which comprises approximately 60% of extant bird species. This review aims to compile the most significant contributions on the DNA/RNA viruses affecting passerines, from traditional and metagenomic studies. It highlights that most passerine species have never been sampled. Especially the RNA viruses from Flaviviridae, Orthomyxoviridae and Togaviridae are considered emerging because of increased incidence or avian mortality/morbidity, spread to new geographical areas or hosts and their zoonotic risk. Arguably poxvirus, and perhaps other virus groups, could also be considered "emerging viruses". However, many of these viruses have only recently been described in passerines using metagenomics and their role in the ecosystem is unknown. Finally, it is noteworthy that only one third of the viruses affecting passerines have been officially recognized.