Person:
Saiz-Pardo Sanz, Melchor

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First Name
Melchor
Last Name
Saiz-Pardo Sanz
URI
https://hdl.handle.net/20.500.14352/78881
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Medicina Legal, Psiquiatría y Patología
Area
Anatomía Patológica
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2022 - 20242
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Author: Enciso, Silvia × Item Type: Publication ×

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    Osteogenic-angiogenic coupled response of cobalt-containing mesoporous bioactive glasses in vivo
    (Acta Biomaterialia, 2024) Jiménez Holguín, Javier; Lozano Borregón, Daniel; Saiz-Pardo Sanz, Melchor; Pablo, David de; Ortega, Luis ; Enciso, Silvia; Fernandez Tome, Blanca; Díaz-Güemes, Idoia; Sanchez Margallo, Francisco Miguel; Portolés Pérez, María Teresa; Arcos Navarrete, Daniel
    The incorporation of cobalt ions into the composition of bioactive glasses has emerged as a strategy of interest for bone regeneration purposes. In the present work, we have designed a set of bioactive mesoporous glasses SiO2 -CaO-P2 O5 -CoO (Co-MBGs) with different amounts of cobalt. The physicochemi- cal changes introduced by the Co2 + ion, the in vitro effects of Co-MBGs on preosteoblasts and endothelial cells and their in vivo behaviour using them as bone grafts in a sheep model were studied. The results show that Co2 + ions neither destroy mesoporous ordering nor inhibit in vitro bioactive behaviour, ex- erting a dual role as network former and modifier for CoO concentrations above 3 % mol. On the other hand, the activity of Co-MBGs on MC3T3-E1 preosteoblasts and HUVEC vascular endothelial cells is de- pendent on the concentration of CoO present in the glass. For low Co-MBGs concentrations (1mg/ml) cell viability is not affected, while the expression of osteogenic (ALP, RUNX2 and OC) and angiogenic (VEGF) genes is stimulated. For Co-MBGs concentration of 5 mg/ml, cell viability decreases as a function of the CoO content. In vivo studies show that the incorporation of Co2 + ions to the MBGs improves the bone regeneration activity of these materials, despite the deleterious effect that this ion has on bone-forming cells for any of the Co-MBG compositions studied. This contradictory effect is explained by the marked increase in angiogenesis that takes place inside the bone defect, leading
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    Injectable mesoporous bioactive nanoparticles regenerate bone tissue under osteoporosis conditions
    (Acta Biomaterialia, 2022) Arcos Navarrete, Daniel; Gómez Cerezo, María Natividad; Saiz-Pardo Sanz, Melchor; Pablo Velasco, David de; Ortega Medina, Luis; Enciso, Silvia; Fernández Tomé, Blanca; Díaz Güemes, Idoia; Sánchez Margallo, Francisco Miguel; Casarrubios Palomar, Luis; Feito Castellano, María José; Portolés Pérez, María Teresa; Vallet Regí, María Dulce Nombre
    The osteogenic capability of mesoporous bioactive nanoparticles (MBNPs) in the SiO2–CaO system has been assessed in vivo using an osteoporotic rabbit model. MBNPs have been prepared using a double template method, resulting in spherical nanoparticles with a porous core-shell structure that has a high surface area and the ability to incorporate the anti-osteoporotic drug ipriflavone. In vitro expression of the pro-inflammatory genes NF-κB1, IL-6, TNF-α, P38 and NOS2 in RAW-264.7 macrophages, indicates that these nanoparticles do not show adverse inflammatory effects. An injectable system has been prepared by suspending MBNPs in a hyaluronic acid-based hydrogel, which has been injected intraosseously into cavitary bone defects in osteoporotic rabbits. The histological analyses evidenced that MBNPs promote bone regeneration with a moderate inflammatory response. The incorporation of ipriflavone into these nanoparticles resulted in a higher presence of osteoblasts and enhanced angiogenesis at the defect site, but without showing significant differences in terms of new bone formation.