Sanz Alonso, Mariano

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First Name
Last Name
Sanz Alonso
Universidad Complutense de Madrid
Faculty / Institute
Especialidades Clínicas Odontológicas
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Now showing 1 - 10 of 29
  • Publication
    Efficacy and safety of a bioadhesive gel containing propolis extract, nanovitamin C and nanovitamin E on desquamative gingivitis: a double‑blind, randomized, clinical trial
    (Springer Nature, 2022-07-28) González Serrano, José; Serrano Valle, Julia; Sanz Alonso, Mariano; Torres García-Denche, Jesús; Hernández Vallejo, Gonzalo; López-Pintor Muñoz, Rosa María
    Objectives: To evaluate the efcacy of a gel-containing propolis extract, nanovitamin C, and nanovitamin E as adjuvants to professional plaque removal on desquamative gingivitis (DG). Materials and methods: A randomized clinical trial was conducted on patients sufering DG due to mucocutaneous diseases. Patients received professional supragingival prophylaxis with oral hygiene instructions and were randomly assigned to use test or control gels as toothpaste and to apply it on DG lesions 3 times/day for 4 weeks. DG clinical score (DGCS), clinical periodontal variables, and visual analog scale (VAS) for pain and oral health impact profle (OHIP-14) were collected at baseline, 2 and 4 weeks. Results: Twenty-two patients were randomly assigned to test (n=11) or control group (n=11). Eighteen had diagnosis of oral lichen planus and four of mucous membrane pemphigoid. DGCS statistically decreased in both groups after treatment with no signifcant diferences between groups. Clinical periodontal outcomes decreased in both groups, but no signifcant diferences were observed. Periodontal variables statistically improved only in test group after treatment. VAS and OHIP-14 scores decreased in test and control groups without signifcant diferences. However, only one test group showed a statistically signifcant decrease in VAS and OHIP-14 scores after treatment. No adverse efects were reported. Conclusions: Test gel may alleviate DG and improve quality of life without side efects. Clinical relevance: A gel-containing propolis extract, nanovitamin C, and nanovitamin E as adjuvants to mechanical debridement may improve both clinical and patient related outcomes in DG patients without side efects. Clinical trial registration. The study protocol was registered at with the following number: NCT05124366 on October 16, 2021.
  • Publication
    Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
    (MDPI, 2022-03-17) Sanchez, Nerea; Vignoletti, Fabio; Sanz Martín, Ignacio; Coca, Alejandro; Núñez, Javier; Maldonado Bautista, Estela; Sanz Esporrín, Javier; Hernando Pradíes, Irene; Santamaría, Silvia; Herrera González, David; Garcia Sanz, Jose A.; Sanz Alonso, Mariano
    (1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were created in seven dogs. GMSCs were isolated, transfected with a vector carrying green fluorescent protein (GFP) and expanded. Once chronified, the defects were randomly treated with (1) CAF plus the combination of CMX and GFP+ GMSCs, (2) CAF plus CMX with autologous fibroblasts, (3) CAF plus CMX and (4) CAF alone. Histological and clinical outcomes at 2- and 6-week healing periods were analyzed and compared among groups. (3) Results: Histologically, the addition of autologous cells to the CMX resulted in reduced inflammation and a variable degree of new cementum/bone formation. CMX plus GMSCs resulted in greater mean recession reduction (1.42; SD = 1.88 mm) and percentage of teeth with recession reduction of ≥2 mm (57%) when compared to the other groups, although these differences were not statistically significant. (4) Conclusions: The histometric and clinical results indicated a positive trend favouring the combination of CMX and GMSCs with the CAF when compared to the groups without cells, although these differences were not statistically significant.
  • Publication
    Cómo motivar al estudiante: Priming y B-learning
    (2017-06-15) Iniesta Albentosa, Margarita Isabel; Alonso Álvarez, Bettina María; Herrera González, David; Sanz Alonso, Mariano; Figuero Ruiz, Elena; Santa Cruz Astorgui, María Isabel; Legido Arce, Berta; Carrillo de Albornoz Sainz, Ana; Dolláguez Chamarro, Patricia
    Este proyecto pretende mejorar el rendimiento de los estudiantes, independientemente de su nivel de conocimientos y, a la vez, mejorar su motivación y su compromiso con la asignatura de Periodoncia. Para ello se construye una serie de actividades pre-clase adaptadas al nivel del 2º curso de Grado en Odontología.
  • Publication
    In vitro effect of different implant decontamination methods in three intraosseous defect configurations
    (Wiley, 2022-05-06) Luengo Mas, Fernando; Sanz Esporrín, Javier; Noguerol, Fernando; Sanz Martín, Ignacio; Sanz Sánchez, Ignacio; Sanz Alonso, Mariano
    Objectives This in vitro investigation was aimed to evaluate the cleaning ability of four mechanical devices designed for decontaminating implant surfaces. Material and methods Ninety-six implants were coated with permanent ink and inserted into 3D-printed resin blocks simulating three different intraosseous defect configurations (types Ib, Ic, and Ie). The four tested mechanical decontamination devices (air-polishing with glycine powder, rotating titanium brush, polyetheretherketone [PEEK]-coated ultrasonic tip, and stainless steel ultrasonic tip) were randomly applied onto the 5 mm exposed implant surface. Standardized photographs were taken from a frontal perspective and with a 30° angle coronally and apically to the implant axis. The area with remnant ink on the implant surface was calculated. Results Although none of the groups achieved complete ink removal, air-polishing with glycine and titanium brushes demonstrated a higher cleaning ability when compared with ultrasonic devices either with standard or PEEK tips for all three defect configurations. For the three tested models, the best cleaning ability in all groups was shown on implant surfaces without facing an intraosseous wall. Titanium brush was the most effective when the intraosseous walls existed. Cleaning effectiveness diminished in the threads located in the apical third, especially when using air-polishing and ultrasonic devices. Conclusions Titanium brushes and air-polishing devices were more effective in removing artificial biofilm using this in vitro model, although their effectiveness was influenced by the presence of the intrabony component.
  • Publication
    Ibero‐Panamerican Federation of Periodontics Delphi study on the trends in diagnosis and treatment of peri‐implant diseases and conditions: A Latin American consensus
    (Wiley, 2021-04-14) Alarcón, Marco Antonio; Sanz Sánchez, Ignacio; López Pacheco, Andrea; Tavelli, Lorenzo; Galarraga Vinueza, Maria Elisa; Schwarz, Frank; Romanelli, Hugo; Peredo, Luis; Pannuti, Claudio Mendes; Javer, Enrique; Vieira, Andrés Felipe; Montealegre, Mauricio; Galindo, Roberto; Umanzor, Vilma; Treviño, Alejandro; Fretes‐Wood, Patricia; Cisneros, Marissa; Collins, James R.; Bueno, Luis; Gimenéz, Xiomara; Málaga Figueroa, Lilian; Sanz Alonso, Mariano
    Background: The social diversity, heterogeneous culture, and inherent economic inequality factors in Latin America (LA) justify conducting a comprehensive analysis on the current status and future trends of peri-implant diseases and conditions. Thus, the aim of this Delphi study was to predict the future trends in the diagnosis and treatment of peri-implant diseases and conditions in LA countries for the year 2030. Methods: A Latin American steering committee and group of experts in implant dentistry validated a questionnaire including 64 questions divided into eight sections. The questionnaire was run twice with an interval of 45 days, with the results from the first round made available to all the participants in the second round. The results were expressed in percentages and data was analyzed describing the consensus level reached in each question. Results: A total of 221 experts were invited to participate in the study and a total 214 (96.8%) completed the two rounds. Moderate (65%-85%) to high consensus (≥85%) was reached in 51 questions (79.69%), except in the questions dealing with “prevalence”, where no consensus was reached. High and moderate consensus was attained for all the questions in three fields (risk factors and indicators, diagnosis and treatment of peri-implant conditions and deficiencies, and prevention and maintenance). Conclusions: The present study has provided relevant and useful information on the predictions in the diagnosis and treatment of peri-implant diseases with a high level of consensus among experts. Nevertheless, there is still a lack of agreement in certain domains.
  • Publication
    Aprender educando: Experiencia ApS de promoción de la salud oral integrada en una asignatura. Proyecto piloto
    (Universidad Complutense de Madrid, 2020) Iniesta Albentosa, Margarita Isabel; Garcillán Izquierdo, María del Rosario; Jiménez de la Hoz, María del Carmen; Alonso Álvarez, Bettina María; Hidalgo Arroquia, Juan José; Figuero Ruiz, Elena; de Arriba de la Fuente, Lorenzo; Herrera González, David; Sanz Alonso, Mariano; Tanarro Colodrón, David; Mateos Moreno, María Victoria; Lamas Oliveira, Marta; Lenguas Silva, Ana Leticia; García Vicent, Germán; Agudo García, María Ángeles; Robaina Tadeo, María Teresa; Darwish Mateos, Leyla; Deza Calzadilla, Anett Michelly; López Muñóz, María; Vargas Labraña, Javiera
    Después de dos años desarrollando la metodología ApS, este proyecto estaba propuesto con la intención de integrar esta metodología en una asignatura de 5º curso del Grado de Odontología. Varios alumnos de la asignatura participaron en el proyecto, pero ante su bajo número se brindó la oportunidad al resto de cursos, para todos aquellos estudiantes que quisieran participar de forma voluntaria. Los objetivos propuestos en el proyecto fueron alcanzados con entera satisfacción.
  • Publication
    Desarrollo de las competencias digitales en el profesorado
    (2021-12-16) Iniesta Albentosa, Margarita Isabel; Pérez-Higueras Sánchez-Escalonilla, Juan José; García Collantes, María Belén; López del Barrio, María Ángeles; Lacasa Otín, María Ángeles; Alonso Álvarez, Bettina María; Figuero Ruiz, Elena; Sanz Alonso, Mariano; Fan, Dandan; Castillo Castillo, Verónica; Bengoechea de la Fuente, Nuria
    Se propuso y se llevó a cabo un plan de acción estratégico para la mejora de las competencias digitales en el profesorado. En él se estableció una acción conjunta por parte de la Facultad de Odontología de la UCM junto con la Biblioteca de dicha Facultad. La acción consistió en la implementación de un programa de cursos de interés para el Personal Docente e Investigador, con el fin de proporcionar herramientas de apoyo a la docencia y de desarrollar estrategias de aprendizaje efectivas, inclusivas e innovadoras.
  • Publication
    Study on the immunopathological effect of titanium particles in peri‐implantitis granulation tissue: A case–control study
    (Wiley, 2022-02-28) Rakic, Mia; Radunovic, Milena; Petkovic Curcin, Aleksandra; Tatic, Zoran; Basta Jovanovic, Gordana; Sanz Alonso, Mariano
    Objectives To identify titanium particles (TPs) in biopsy specimens harvested from peri-implantitis lesions and secondarily to study the histopathological characteristics in peri-implantitis compared to periodontitis, in order to evaluate whether the presence of TPs could alter respective inflammatory patterns. Material and methods Biopsies containing granulation tissue were harvested during routine surgical treatment in 39 peri-implantitis cases and 35 periodontitis controls. Serial sections were obtained using titanium-free microtome blades. The first and last sections of the peri-implantitis specimens were used for identification of TPs by scanning electron microscopy coupled with dispersive X-ray spectrometry. Intermediate sections and periodontitis specimens were processed for descriptive histological study using haematoxylin–eosin staining and for immunohistochemical analysis using CD68, IL-6, Nf-kB and VEGF markers. Results TPs were identified in all peri-implantitis specimens as free metal bodies interspersed within granulation tissue. However, presence of macrophages or multinucleated giant cells engulfing the TPs were not identified in any specimen. Peri-implantitis granulations were characterized by a chronic inflammatory infiltrate rich in neutrophils. About half of peri-implantitis patients exhibited a subacute infiltrate characterized with lymphocytes interweaved with neutrophils and eosinophils. When compared to periodontitis, peri-implantitis tissues showed higher proportions of macrophages and a more intense neovascularization, based on significantly higher expression of CD68 and VEGF respectively. Conclusion TPs were identified in all peri-implantitis specimens, but without evidencing any foreign body reaction suggestive for direct pathological effects of TPs. The peri-implantitis granulation tissue was characterized by intense neovascularization and presence of a chronic inflammatory infiltrate dominated by plasma cells, neutrophils and macrophages.
  • Publication
    Aprender educando a personas con discapacidad: experiencia de Aprendizaje Servicio en Odontología y Magisterio
    (Centro Superior de Estudios Universitarios La Salle, 2020-09-30) Iniesta Albentosa, Margarita, (coord.); Alonso Álvarez, Bettina María; Carrillo de Albornoz Sainz, Ana; Figuero Ruiz, Elena; Herrera González, David; Hidalgo Arroquia, Juan José; Jiménez de la Hoz, María del Carmen; Matesanz Pérez, Paula; Sanz Alonso, Mariano; Sanz Sánchez, Ignacio
    Este texto es fruto de un Proyecto de Innovación Docente de la convocatoria 2018-2019, llevado a cabo en la Facultad de odontología de la UCM. El documento desarrolla la metodología ApS desde un punto de vista metodológico, y está estructurado en dos partes. En la primera parte se plantean las necesidades que cubre este tipo de aprendizaje, sobre todo en el área de las Ciencias de la Salud, y las competencias clave que, de forma general, puede ayudar a desarrollar. Además, se ha intentado realizar una guía para marcar las directrices necesarias a la hora de elaborar las distintas fases de este tipo de proyecto. En la segunda parte se describe la experiencia práctica llevada a cabo por un equipo interprofesional formado por estudiantes de Odontología de la UCM y de Educación de la Salle.
  • Publication
    De novo bone formation around implants with a surface based on a monolayer of multi‐phosphonate molecules. An experimental in vivo investigation
    (Wiley, 2021-06-17) Sanz Esporrin, Javier; Di Raimondo, Riccardo; Vignoletti, Fabio; Núñez, Javier; Muñoz, Fernando; Sanz Alonso, Mariano
    Objectives: The purpose of this experimental in vivo investigation was to evaluate the influence of modifying the implant surface by adding a monolayer of multiphosphonate molecules on the de novo bone formation and osseointegration. Material and Methods: The study was designed as an animal preclinical trial with intra-animal control and two healing periods, 2 and 8 weeks, to compare implants with an identical macro-design but with two different surfaces. Eight female Beagle dogs participated in the study. Control implants had a moderately rough surface combining sandblasting and acid etching; test implants had an additional monophosphonate layer covalently bonded to titanium. Histologic and radiographic (micro-CT) outcome variables were evaluated. Results: The first bone-to-implant contact (fBIC) was located more coronally for the test implants at the first (0.065 mm (95% CI = −0.82, 0.60)) and second healing milestones (0.17 mm (95% CI = −0.9, 0.55)). Most coronal BIC of the test implants displayed a higher percentage of osseointegration, +6.33% and +13.38% after 2 and 8 weeks, respectively; however, the differences were not statistically significant. The micro-CT examination did not show any BIC difference. Conclusions: The monophosphonate layer coating demonstrated clinical, histological, and radiographic results similar to the control surface.