Person:
Martínez Ibáñez, María Dolores

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First Name
María Dolores
Last Name
Martínez Ibáñez
URI
https://hdl.handle.net/20.500.14352/83506
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Area
Zoología
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UCM identifierDialnet ID

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2005 - 20072
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    The expression of GLP‐1 receptor mRNA and protein allows the effect of GLP‐1 on glucose metabolism in the human hypothalamus and brainstem
    (Journal of Neurochemistry, 2005) Álvarez García, Elvira; Martínez Ibáñez, María Dolores; Roncero Rincón, Isabel; Chowen, Julie A.; García‐Cuartero, Beatriz; Gispert, Juan D.; Sanz Miguel, María Del Carmen; Vázquez Pérez, Patricia; Antonio, Maldonado; De Cáceres, Javier; Desco, Manuel; Pozo García, Miguel Ángel; Blázquez Fernández, Enrique
    In the present work, several experimental approaches were used to determine the presence of the glucagon-like peptide-1 receptor (GLP-1R) and the biological actions of its ligand in the human brain. In situ hybridization histochemistry revealed specific labelling for GLP-1 receptor mRNA in several brain areas. In addition, GLP-1R, glucose transporter isoform (GLUT-2) and glucokinase (GK) mRNAs were identified in the same cells, especially in areas of the hypothalamus involved in feeding behaviour. GLP-1R gene expression in the human brain gave rise to a protein of 56 kDa as determined by affinity cross-linking assays. Specific binding of 125I-GLP-1(7-36) amide to the GLP-1R was detected in several brain areas and was inhibited by unlabelled GLP-1(7-36) amide, exendin-4 and exendin (9-39). A further aim of this work was to evaluate cerebral-glucose metabolism in control subjects by positron emission tomography (PET), using 2-[F-18] deoxy-D-glucose (FDG). Statistical analysis of the PET studies revealed that the administration of GLP-1(7-36) amide significantly reduced (p < 0.001) cerebral glucose metabolism in hypothalamus and brainstem. Because FDG-6-phosphate is not a substrate for subsequent metabolic reactions, the lower activity observed in these areas after peptide administration may be due to reduction of the glucose transport and/or glucose phosphorylation, which should modulate the glucose sensing process in the GLUT-2- and GK-containing cells.
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    Aula virtual de prácticas de Entomología Ambiental y Aplicada
    (III jornada Campus Virtual UCM : Innovación en el Campus Virtual metodologías y herramientas, 2007) Vázquez Martínez, María Ángeles; Outerelo Domínguez, Raimundo; Mínguez Martínez, María Eugenia; Martínez Ibáñez, María Dolores; Gamarra, Purificación; Ruiz Piña, Eduardo; Hernández de Miguel, José María; Fernández-Valmayor Crespo, Alfredo; Fernández-Pampillón Cesteros, Ana María; Merino Granizo, Jorge
    Las prácticas de la asignatura «Entomología Ambiental y Aplicada», correspondiente al segundo ciclo de la licenciatura en CC. Biológicas tienen como objetivo el conocimiento de especies de artrópodos que constituyen plaga o causan beneficios. Para alcanzar éste en el laboratorio se observan ejemplares de artrópodos de interés económico y sanitario, así como otras especies útiles. La manipulación del material necesario, no siempre fácil de conseguir y preparar, produce muchas roturas y pérdidas debido a su fragilidad. Nombre científico, Nombre común, Caracteres diagnósticos, Daños producidos (que en el bloque beneficiosos se debe entender como efectos beneficiosos), Grupos sistemáticos en los que se sitúa la especie, Fenología.