Bañares Cañizares, Rafael

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Bañares Cañizares
Universidad Complutense de Madrid
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Now showing 1 - 7 of 7
  • Publication
    Fat: Quality, or Quantity? What Matters Most for the Progression of Metabolic Associated Fatty Liver Disease (MAFLD)
    (MDPI, 2021-09-22) Estévez-Vázquez, Olga; Benedé-Ubieto, Raquel; Guo, Feifei; Gómez-Santos, Beatriz; Aspichueta, Patricia; Reissing, Johanna; Bruns, Tony; Sanz-García, Carlos; Sydor, Svenja; Bechmann, Lars P; Maranillo Alcaide, Eva; Sañudo Tejero, José Ramón; Vázquez Osorio, María Teresa; Lamas-Paz, Arantza; Morán, Laura; Mazariegos, Marina S; Ciudin, Andreea; Pericàs, Juan M.; Peligros, María Isabel; Vaquero, Javier; Martínez-Naves, Eduardo; Liedtke, Christian; Regueiro González-Barros, José Ramón; Trautwein, Christian; Bañares Cañizares, Rafael; Cubero Palero, Francisco Javier; Nevzorova, Yulia A.
    Objectives: Lately, many countries have restricted or even banned transfat, and palm oil has become a preferred replacement for food manufacturers. Whether palm oil is potentially an unhealthy food mainly due to its high content of saturated Palmitic Acid (PA) is a matter of debate. The aim of this study was to test whether qualitative aspects of diet such as levels of PA and the fat source are risk factors for Metabolic Syndrome (MS) and Metabolic Associated Fatty Liver Disease (MAFLD). Methods: C57BL/6 male mice were fed for 14 weeks with three types of Western diet (WD): 1. LP-WD—low concentration of PA (main fat source—corn and soybean oils); 2. HP-WD—high concentration of PA (main fat source—palm oil); 3. HP-Trans-WD—high concentration of PA (mainly transfat). Results: All types of WD caused weight gain, adipocyte enlargement, hepatomegaly, lipid metabolism alterations, and steatohepatitis. Feeding with HP diets led to more prominent obesity, hypercholesterolemia, stronger hepatic injury, and fibrosis. Only the feeding with HP-Trans-WD resulted in glucose intolerance and elevation of serum transaminases. Brief withdrawal of WDs reversed MS and signs of MAFLD. However, mild hepatic inflammation was still detectable in HP groups. Conclusions: HP and HP-Trans-WD play a crucial role in the genesis of MS and MAFLD.
  • Publication
    Sinusoidal obstruction syndrome after liver transplantation: A multicenter observational study
    (Wiley, 2022-03-07) Caballero Marcos, Aránzazu; Peligros, Isabel; Pérez Rojas, Judith; Campos Varela, Isabel; Colmenero, Jordi; Gómez Bravo, Miguel Ángel; Justo, Iago; Otero, Alejandra; Molina Pérez, Esther; González Diéguez, Luisa; Baliellas, Carme; Romero Cristobal, Mario; Aguilera, Victoria; Castells, Lluís; Díaz, Alba; Marín Gómez, Luis Miguel; Loinaz, Carmelo; Bañares Cañizares, Rafael; Salcedo, Magdalena
    Diagnosis of sinusoidal obstruction syndrome (SOS) after hematopoietic cell transplantation (HCT) is based on clinical criteria including weight gain, ascites, hepatomegaly, and jaundice.[1] However, clinical and histological features and prognosis of SOS after liver transplantation (LT) seem to differ from SOS after HCT.[2, 3] We aimed to determine the characteristics and outcomes of SOS after LT.
  • Publication
    Lack of Cyclin E1 in hepatocytes aggravates ethanol-induced liver injury and hepatic steatosis in experimental murine model of acute and chronic alcohol-associated liver disease
    (Elsevier, 2023-02-01) Ramadori, Pierluigi; Woitok, Marius Maximilian; Estévez Vázquez, Olga; Benede Ubieto, Raquel; Leal Lassalle, Héctor; Lamas Paz, Arantza; Guo, Feifei; Fabre, Jeanne; Otto, Julia; Verwaayen, Anna; Reissing, Johanna; Bruns, Tony; Erschfeld, Stephanie; Haas, Ute; Paffen, Daniela; Nelson, Leonard J.; Vaquero Martín, Francisco Javier; Bañares Cañizares, Rafael; Trautwein, Christian; Cubero Palero, Francisco Javier; Liedtke, Christian; Nevzorova, Yulia
    Background: Cyclin E1 is the regulatory subunit of cyclin-dependent kinase 2 (Cdk2) and one of the central players in cell cycle progression. We recently showed its crucial role for initiation of liver fibrosis and hepatocarcinogenesis. In the present study, we investigated the role of Cyclin E1 in the development of alcohol-associated liver disease (ALD). Methods: Mice with constitutive (E1-/-), hepatocyte-specific (Cyclin E1Δhepa), or intestinal-epithelial-cell-specific (Cyclin E1ΔIEC) inactivation of Cyclin E1 and corresponding wild type littermate controls (WT) were administered either a Lieber-DeCarli ethanol diet (LDE) for 3 weeks or acute ethanol binges (6 g/kg) through oral gavage. Serum parameters of liver functionality were measured; hepatic tissues were collected for biochemical and histological analyses. Results: The administration of acute EtOH binge and chronic LDE diet to E1-/- mice enhanced hepatic steatosis, worsened liver damage and triggered body weight loss. Similarly, in the acute EtOH binge model, Cyclin E1Δhepa mice revealed a significantly worsened liver phenotype. In contrast, inactivation of Cyclin E1 only in intestinal epithelial cell (IECs)did not lead to any significant changes in comparison to WT mice after acute EtOH challenge. Remarkably, both acute and chronic EtOH administration in E1-/- animals resulted in increased levels of ADH and decreased expression of ALDH1/2. The additional application of a pan-Cdk inhibitor (S-CR8) further promoted liver damage in EtOH-treated WT mice. Conclusion: Our data point to a novel unexpected role of Cyclin E1 in hepatocytes for alcohol metabolism, which seems to be independent of the canonical Cyclin E1/Cdk2 function as a cell cycle regulator.
  • Publication
    Aprendizaje de las habilidades no técnicas en las nuevas generaciones de estudiantes de medicina: nueva estrategia docente en un escenario bélico real
    (2019-03-29) Quintana Villamandos, María Begoña; Colado Megia, María Isabel; Barrigón Vázquez, Santos; Bañares Cañizares, Rafael; Vicente Crespo, María Elena; Benito Mateo, Miguel
    Establecer una nueva estrategia docente en el aprendizaje de las habilidades no técnicas (conciencia situacional, tomar decisiones, comunicación, trabajo en equipo y liderazgo) del estudiante en un escenario real reforzaría sus habilidades técnicas.
  • Publication
    Innovación en la docencia del grado de medicina: Aerotransporte de pacientes con alto riesgo de contaminación por enfermedad infecciosa en un escenario real
    (2021) Quintana Villamandos, Begoña; Colado Megía, María Isabel; Barrigón Vazquez, Santos; Bañares Cañizares, Rafael; del Cañizo López, Juan Francisco; Vicente Crespo, María Elena; Martínez Belotto, Miguel
  • Publication
    A Shortcut from Metabolic-Associated Fatty Liver Disease (MAFLD) to Hepatocellular Carcinoma (HCC): c-MYC a Promising Target for Preventative Strategies and Individualized Therapy
    (MDPI, 2021-12-31) Guo, Feifei; Estevez Vázquez, Olga; Benedé Ubieto, Raquel; Maya Mile, Douglas; Zheng, Kang; Gallego Durán, Rocío; Rojas Ávalos, Ángela; Ampuero, Javier; Romero Gómez, Manuel; Philip, Kaye; Egbuniwe, Isioma U.; Chen, Chaobo; Simon, Jorge; Delgado, Teresa C.; Martínez Chantar, Maria L.; Sun, Jie; Reissing, Johanna; Bruns, Tony; Lamas Paz, Arantza; Gómez del Moral Martín-Consuegra, Manuel María; Woitok, Marius Maximilian; Vaquero Martín, Javier; Regueiro, José R.; Liedtke, Christian; Trautwein, Christian; Bañares Cañizares, Rafael; Cubero Palero, Francisco Javier; Nevzorova, Yulia A.
    Background: Metabolic-associated fatty liver disease (MAFLD) has risen as one of the leading etiologies for hepatocellular carcinoma (HCC). Oncogenes have been suggested to be responsible for the high risk of MAFLD-related HCC. We analyzed the impact of the proto-oncogene c-MYC in the development of human and murine MAFLD and MAFLD-associated HCC. Methods: alb-myctg mice were studied at baseline conditions and after administration of Western diet (WD) in comparison to WT littermates. c-MYC expression was analyzed in biopsies of patients with MAFLD and MAFLD-associated HCC by immunohistochemistry. Results: Mild obesity, spontaneous hyperlipidaemia, glucose intolerance and insulin resistance were characteristic of 36-week-old alb-myctg mice. Middle-aged alb-myctg exhibited liver steatosis and increased triglyceride content. Liver injury and inflammation were associated with elevated ALT, an upregulation of ER-stress response and increased ROS production, collagen deposition and compensatory proliferation. At 52 weeks, 20% of transgenic mice developed HCC. WD feeding exacerbated metabolic abnormalities, steatohepatitis, fibrogenesis and tumor prevalence. Therapeutic use of metformin partly attenuated the spontaneous MAFLD phenotype of alb-myctg mice. Importantly, upregulation and nuclear localization of c-MYC were characteristic of patients with MAFLD and MAFLD-related HCC. Conclusions: A novel function of c-MYC in MAFLD progression was identified opening new avenues for preventative strategies.
  • Publication
    Aeroevacuación en un escenario real: nueva herramienta de aprendizaje en las futuras generaciones de estudiantes de Medicina
    (2020-01-09) Quintana Villamandos, María Begoña; Colado Megía, María Isabel; Barrigón Vázquez, Santos; Cañizo López, Juan Francisco Del; Martínez Belotto, Miguel; Bañares Cañizares, Rafael; Pinilla Iribar, Paula
    La aeroevacuación en un escenario real podría ser una nueva herramienta de aprendizaje en las futuras generaciones de estudiantes de sexto de medicina, no siendo tan eficaz en los estudiantes de cursos inferiores.