Person:
Pérez Uz, María Blanca

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First Name
María Blanca
Last Name
Pérez Uz
URI
https://hdl.handle.net/20.500.14352/74448
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Genética, Fisiología y Microbiología
Area
Microbiología
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Author: Serrano Barrero, Susana Lourdes ×

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Now showing 1 - 6 of 6
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    Project number: 106
    Diseño y utilización de unas prácticas de Regulación del Metabolismo como herramienta integradora de conocimientos multidisciplinares en el Grado en Biología (II)
    (2017) Guillén Maestro, Alberto; Pérez Uz, María Blanca; Serrano Barrero, Susana Lourdes; Arregui García-Rovés, Lucía; Callejas Hervás, Carmen; Linacero de la Fuente, Rosario; Acebal Sarabia, Carmen; Megías Fresno, Alicia; Gómez Miguel, Begoña
    Se pretende la creación de espacios multidisdiplinares en el Grado en Biología,con prácticas integradas de asignaturas que se cursan simultáneamente, que pudieran servir de base a propuestas de Trabajos de Fin de Grado.
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    Project number: 24
    Diseño y utilización de unas prácticas de Regulación del Metabolismo como herramienta integradora de conocimientos multidisciplinares en el Grado en Biología
    (2015) Guillén Maestro, Alberto; Acebal Sarabia, Carmen; Callejas Hervás, Carmen; Gómez Miguel, Begoña; Linacero de la Fuente, María Rosario; Megías Fresno, Alicia; Pérez Uz, María Blanca; Serrano Barrero, Susana Lourdes
    Con el diseño de estas prácticas de laboratorio se pretende la creación de espacios multidisdiplinares en el Grado en Biología que puedieran dar paso a un futuro modelo de prácticas integradas de asignaturas que se cursan simultáneamente.
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    Project number: 110
    La curación de contenidos científicos como estrategia didáctica y divulgativa en Microbiología y otras áreas afines
    (2020) Gil-Serna, Jéssica; Arregui García-Rovés, Lucía; Guillen Maestro, Alberto; Martín Cereceda, María Mercedes; Pérez Uz, María Blanca; Serrano Barrero, Susana Lourdes; Silóniz Jiménez, María Isabel de; Vázquez Estévez, María Covadonga Inmaculada; Patiño Álvarez, Aurora Belén
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    Ciliate contributions to bioaggregation: laboratory assays with axenic cultures of Tetrahymena thermophila
    (INTERNATIONAL MICROBIOLOGY, 2007) Arregui García-Roves, Lucía; Serrano Barrero, Susana Lourdes; Linares Gómez, María; Pérez Uz, María Blanca; Guinea Díaz, Almudena
    Protists, mainly ciliates, play several essential roles in biological wastewater treatment, such as the transfer of matter and energy, bacterial predation, and the removal of organic material. Moreover, during the treatment process, the formation of bioaggregates—flocs and biofilms—is essential to obtaining high-quality effluents. In the present study, Tetrahymena thermophila was used as a model organism to demonstrate the contribution of ciliates to bioflocculation. Axenic cultures of this species were exposed to chemical and mechanical stimuli that promote bioaggregation. In either case, the secretion of a capsulate mucous material by the ciliates or by particle aggregation was detected. Numerous, small, loosely compacted flocs were observed under shaking conditions and in the presence of latex beads. The composition of the exopolymeric material secreted by ciliates was analyzed by a series of fluorochromes and colorimetric methods, which showed that carbohydrates and nucleic acids were the main components involved in matrix formation and particle adhesion.
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    Project number: 136
    La Microbiología a debate
    (2021) Gil Serna, Jéssica; Arregui García-Rovés, Lucía; Belda Aguilar, Ignacio; Martín Cereceda, María Mercedes; Patiño Álvarez, Aurora Belén; Pérez Uz, María Blanca; Serrano Barrero, Susana Lourdes; Vázquez Estévez, María Covadonga
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    Involvement of Crawling and Attached Ciliates in the Aggregation of Particles in Wastewater Treatment Plants
    (Air, Soil and Water Research, 2008) Arregui García-Roves, Lucía; Linares Gómez, María; Pérez Uz, María Blanca; Guinea Díaz, Almudena; Serrano Barrero, Susana Lourdes
    The biological community in activated sludge wastewater plants is organized within this ecosystem as bioaggregates or flocs, in which the biotic component is embedded in a complex matrix comprised of extracellular polymeric substances mainly of microbial origin. The aim of this work is to study the role of different floc-associated ciliates commonly reported in wastewater treatment plants-crawling Euplotes and sessile Vorticella- in the formation of aggregates. Flocs, in experiments with ciliates and latex beads, showed more compactation and cohesion among particles than those in the absence of ciliates. Ciliates have been shown to contribute to floc formation through different mechanisms such as the active secretion of polymeric substances (extrusomes), their biological activities (movement and feeding strategies), or the cysts formation capacity of some species. Staining with lectins coupled to fluorescein showed that carbohydrate of the matrix contained glucose, manose, N-acetyl-glucosamine and galactose. Protein fraction revealed over the latex beads surfaces could probably be of bacterial origin, but nucleic acids represented an important fraction of the extracellular polymeric substances of ciliate origin.