Person:
Vivanco Martínez, Fernando

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First Name
Fernando
Last Name
Vivanco Martínez
URI
https://hdl.handle.net/20.500.14352/84150
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Area
Bioquímica y Biología Molecular
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2010 - 20124
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    Identification of sole parvalbumin as a major allergen: study of cross‐reactivity between parvalbumins in a Spanish fish‐allergic population
    (Clin Exp Allergy, 2011) Perez‐Gordo, M.; Cuesta‐Herranz, J.; Maroto, A. S.; Cases, B.; Ibáñez, M. D.; Vivanco Martínez, Fernando; Pastor Vargas, Carlos
    Background: Fish allergy is becoming an important health problem in Spain, a country with the third highest level of fish consumption after Japan and Portugal. The most common fish allergens are parvalbumins. In our area, the most widely consumed fish species are lean, such as whiff (Lepidorhombus whiffiagonis) and sole (Solea solea). Adverse reactions to fish are usually related to these species, a fact that is largely unknown to allergists in other countries. Objective: The aim of this study was to identify and purify the major allergen implicated in allergic response to sole and evaluate the IgE cross-reactivity of purified parvalbumins from whiff and sole, which are phylogenetically close, and more distant species (i.e. cod and salmon). Methods: Eighteen Spanish fish-allergic patients with a positive history of type I allergy to fish were recruited from the clinic. Total protein extracts and purified parvalbumins from whiff and sole were tested for their IgE-binding properties by combining two-dimensional Western blotting and mass spectrometry. The extent of cross-reactivity between these parvalbumins along with cod and salmon parvalbumins was investigated by IgE ELISA inhibition assay. Results: An IgE-binding spot of approximately 14 kDa was identified as parvalbumin and confirmed as a major allergen in sole extract, which is recognized by almost 70% of the patients. Whiff parvalbumin was recognized by 83.4% of the patients. High cross-reactivity was determined for all purified parvalbumins by IgE inhibition assay. Conclusions and clinical relevance: Sole and whiff parvalbumin were confirmed as major allergens. The parvalbumins of sole, whiff, cod and salmon were highly cross-reactive, thus suggesting a high amino acid sequence identity between them.
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    Anaphylaxis due to orange soft drinks
    (J Investig Allergol Clin Immunol, 2012) Navarro,, L A; Pastor Vargas, Carlos; Liñana, J J; Martinez, I; Sanz Maroto, Aroa; Vivanco Martínez, Fernando; Bartolome, Borja
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    Arginine Kinase from the Cellar Spider (Holocnemus pluchei): A New Asthma-Causing Allergen
    (Int Arch Allergy Immunol, 2010) Bobolea, Irina; Barranco, Pilar; Pastor Vargas, Carlos; Iraola, Victor; Vivanco Martínez, Fernando; Quirce, Santiago
    Background: We report a 31-year-old farmer whose work consists in handling cereal and vegetables, who consulted our clinic because of asthma symptoms after inhalation of dust during manipulation of the deposited material, usually inside the warehouse. Methods and results: Skin prick tests and specific immunoglobulin E (IgE) determinations were negative with common aeroallergens. The patient noted the presence of many spiders in the warehouse, which were identified as the cellar spider Holocnemus pluchei and the common house spider, Tegenaria domestica. Extracts of spider bodies brought in by the patient were obtained and used to perform in vivo and in vitro studies. Molecular characterization of IgE-binding bands was performed by mass spectrometry. We obtained positive prick tests to the extracts of the bodies of both spiders. Immunoblotting displayed different bands in both spider extracts, in a range of 20-70 kDa. All were hemocyanins, except for a 17-kDa protein of Holocnemus identified as an arginine kinase (AK). Bronchial challenge was positive with the extract of the cellar spider and with the AK, but was negative with the domestic house spider. Conclusion: We present the first case of respiratory allergy due to sensitization to AK from a common spider, confirmed by bronchial provocation tests.
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    Asthma After Chicken Consumption due to Cross-reactivity Between Fish and Chicken Parvalbumin
    (J Investig Allergol Clin Immunol, 2012) González-de-Olano, David; Bartolomé, Borja; Sanz Maroto, Aroa; Vivanco Martínez, Fernando; Pastor Vargas, Carlos