Soto Rueda, Juan Manuel

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Juan Manuel
Last Name
Soto Rueda
Universidad Complutense de Madrid
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Now showing 1 - 9 of 9
  • Publication
    Partially coherent optical diffraction tomography for label-free quantitative microscopy
    (IEEE, 2017-09-18) Soto Rueda, Juan Manuel; Rodrigo Martín-Romo, José Augusto; Alieva, Tatiana Krasheninnikova
    In this work we present a tomographic technique for label-free quantitative microscopy that exploits the benefits of the partially coherent illumination. In contrast to conventional holographic techniques based on the coherent optical diffraction tomography (C-ODT), our approach uses an inexpensive non-interferometric setup comprising an electrically tunable lens and low spatially coherent illumination. This setup provides the refocusing needed for axially scan the sample and eventually producing a 3D stack of intensity images. Moreover, the partially coherent illumination avoids speckle noise, which plagues the C-ODT imaging methods. This technique has been developed for optically weak and almost non-absorbing objects that meet the conditions of the first Born approximation. We experimentally demonstrate the capability of the proposed technique by recovering the 3D refractive index distribution of biological samples such as blood cells and diatoms. The results are similar to those obtained by the C-ODT but using a simplified reconstruction process and a refocusing module that can be easily included in commercial widefield microscopes.
  • Publication
    Refractive index tomography for drug-induced liver injury analysis
    (Society of Photo-Optical Instrumentation Engineers (SPIE), 2021-12-07) Soto Rueda, Juan Manuel; Aguilar Mora, Fabio; Rodrigo Martín-Romo, José Augusto; Geng, Yana; Musheshe, Nshunge; Buist-Homan, Manon; Lezoualc’h, Frank; Cheng, Xiaodong; Schmidt, Martina; Moshage, Han; Alieva, Tatiana Krasheninnikova
    Refractive index tomography compatible with conventional microscope is used for analyzing primary rat hepatocytes injury induced by pharmacological treatments. We found that mitochondria malfunctioning is correlated with refractive index variations.
  • Publication
    Optical diffraction tomography with fully and partially coherent illumination in high numerical aperture label-free microscopy
    (Optical Society Of America, 2018-01-01) Soto Rueda, Juan Manuel; Rodrigo Martín-Romo, José Augusto; Alieva, Tatiana Krasheninnikova
    Quantitative label-free imaging is an important tool for the study of living microorganisms that, during the last decade, has attracted wide attention from the optical community. Optical diffraction tomography (ODT) is probably the most relevant technique for quantitative label-free 3D imaging applied in wide-field microscopy in the visible range. The ODT is usually performed using spatially coherent light illumination and specially designed holographic microscopes. Nevertheless, the ODT is also compatible with partially coherent illumination and can be realized in conventional wide-field microscopes by applying refocusing techniques, as it has been recently demonstrated. Here, we compare these two ODT modalities, underlining their pros and cons and discussing the optical setups for their implementation. In particular, we pay special attention to a system that is compatible with a conventional wide-field microscope that can be used for both ODT modalities. It consists of two easily attachable modules: the first for sample illumination engineering based on digital light processing technology; the other for focus scanning by using an electrically driven tunable lens. This hardware allows for a programmable selection of the wavelength and the illumination design, and provides fast data acquisition as well. Its performance is experimentally demonstrated in the case of ODT with partially coherent illumination providing speckle-free 3D quantitative imaging.
  • Publication
    Ampliación y actualización de las prácticas del laboratorio de Instrumentación Biomédica buscando un aprendizaje y evaluación centrados en el estudiante
    (2020-06-19) Chevalier del Rio, Margarita; Alieva Krasheninnikova, Tatiana; Martínez Matos, Oscar; Rodrigo Martín-Romo, Jose Augusto; Canabal Boutureira, Hector Alfonso; Comas Renfigo, María Dolores; Soto Rueda, Juan Manuel; Angulo Curto, Mercedes; Castillo García, María
    El objetivo del proyecto es el diseño de las prácticas de laboratorio para la asignatura Instrumentación Biomédica del Master de Física Biomédica. Se pretende mejorar el aprendizaje potenciando la participación activa y autonomía de los estudiantes. La falta de financiación económica se ha traducido en obtener como resultado del proyecto la redacción de guiones de prácticas que posibiliten el trabajo individual del alumno fuera del laboratorio dado el alto número de alumnos por puesto para llevar a cabo las prácticas. Estos guiones se han complementado con scripts en MatLab para analizar y procesar los datos obtenidos en el laboratorio así como datos obtenidos en diversas bases. Esta propuesta dió información sobre el progreso de los estudiantes en el aprendizaje cuando se utilizó el sistema de rúbricas tambien resultado de este proyecto.
  • Publication
    Label-free quantitative 3D tomographic imaging for partially coherent light microscopy
    (Optical Soc Amer, 2017-07-10) Soto Rueda, Juan Manuel; Rodrigo Martín-Romo, José Augusto; Alieva, Tatiana Krasheninnikova
    Crucial benefits provided by partially coherent light microscopy such as improved spatial resolution, optical sectioning and speckle-noise suppression are exploited here to achieve 3D quantitative imaging: reconstruction of the object refractive index (RI). We present a partially coherent optical diffraction tomography technique (PC-ODT) that can be easily implemented in commercially available bright-field microscopes. We show that the high numerical apertures of the objective and condenser lenses, together with optical refocusing, are main issues for achieving fast and successful 3D RI reconstruction of weak objects. In particular, the optical refocusing is performed by a high-speed focus tunable lens mounted in front of the digital camera enabling compatibility with commercial microscopes. The technique is experimentally demonstrated on different examples: diatom cells (biosilica shells), polystyrene micro-spheres and blood cells. The results confirm the straightforward 3D-RI reconstruction of the samples providing valuable quantitative information for their analysis. Thus, the PC-ODT can be considered as an efficient and affordable alternative to coherent ODT which requires specially designed holographic microscopes.
  • Publication
    Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes
    (Society of Photo-Optical Instrumentation Engineers (SPIE), 2019) Rodrigo Martín-Romo, José Augusto; Soto Rueda, Juan Manuel; Alieva, Tatiana Krasheninnikova
    Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limitedangle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a non-homogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.
  • Publication
    Label-free bioanalysis of Leishmania infantum using refractive index tomography with partially coherent illumination
    (WILEY‐VCH Verlag GmbH, 2019-09) Soto Rueda, Juan Manuel; Rodrigo Martín-Romo, José Augusto; Mas, Alicia; Domínguez Bernal, Gustavo; Alieva, Tatiana Krasheninnikova
    In this work, we report the use of refractive index (RI) tomography for quantitative analysis of unstained DH82 cell line infected with Leishmania infantum. The cell RI is reconstructed by using a modality of optical diffraction tomography technique that employs partially coherent illumination, thus enabling inherent compatibility with conventional wide-field microscopes. The experimental results demonstrate that the cell dry mass concentration (DMC) obtained from the RI allows for reliable detection and quantitative characterization of the infection and its temporal evolution. The RI provides important insight for studying morphological changes, particularly membrane blebbing linked to an apoptosis (cell death) process induced by the disease. Moreover, the results evidence that infected DH82 cells exhibit a higher DMC than healthy samples. These findings open up promising perspectives for clinical diagnosis of Leishmania.
  • Publication
    Partially coherent optical diffraction tomography toward practical cell study
    (Frontiers Media S.A, 2021-06-21) Soto Rueda, Juan Manuel; Rodrigo Martín-Romo, José Augusto; Alieva, Tatiana Krasheninnikova
    Optical diffraction tomography (ODT) is a computational imaging technique based on refractive index (RI) contrast. Its application for microscopic imaging of weakly absorbing and scattering samples has been demonstrated by using a specially designed holographic microscope with angular scanning of the coherent sample illumination direction. Recently, an alternative low cost technique based on partially coherent sample illumination (PC-ODT), which is compatible with the conventional wide-field transmission microscope, has been established. In this case, the 3D refractive index distribution of the sample is obtained by deconvolution from a single stack of through-focus intensity images. The performance of PC-ODT has been successfully tested on various fixed specimens (diatom frustule and biological cells) and moving bacteria. Here, we demonstrate that the PC-ODT is an efficient tool for the analysis of living eukaryotic cell dynamics at short- and long-term periods. The COS-7 cells, which hail from the African green monkey kidney, have been chosen for this study. A fast data acquisition setup comprising an optical scanning module can be easily attached to the microscope, and it allows observing cell 3D organelle movements and RI variations, with the required temporal resolution. In particular, a more rapid nucleoli rotation than previously reported has been found. The long-term cell monitoring during necrosis reveals significant changes in cell dry mass concentration obtained from recovered RI contrast.
  • Publication
    Quantitative imaging in 3D microscopy with partially coherent illumination
    (Universidad Complutense de Madrid, 2021-05-20) Soto Rueda, Juan Manuel; Rodrigo Martín-Romo, José Augusto; Alieva Krasheninnikova, Tatiana
    The main research objective of this work is to develop and implement a fast and high resolution technique for reconstructing the 3D refractive index (RI) of weakly absorbing samples. This technique is based on the generalization of optical diffraction tomography (ODT), developed for coherent sample illumination (C-ODT), to the partially coherent case (PC-ODT). In contrast to C-ODT, which involves a specially designed holographic microscope, the proposed PC-ODT technique is implemented in conventional wide-field microscope. The incorporation of the required refocusing module is easy and can be done by a final user unfamiliar with optics (i.e. clinicians and biologists). The low coherent illumination provided by high numerical aperture condenser results in high resolution and reduced speckle noise typical for laser sources used in C-ODT...