Person:
Prieto Ocejo, Dolores

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First Name
Dolores
Last Name
Prieto Ocejo
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Fisiología
Area
Fisiología
Identifiers
UCM identifierORCIDScopus Author IDWeb of Science ResearcherIDDialnet ID

Search Results

Now showing 1 - 10 of 26
  • Publication
    Impacto de la implementación del aprendizaje basado en problemas en combinación con las prácticas de laboratorio clínico y virtual de Fisiopatología para el desarrollo de competencias profesionales
    (2020-07-01) López-Oliva Muñoz, María Elvira; García Sacristán, Albino; Rivera de los Arcos, Luis; Prieto Ocejo, Dolores; Benedito Castellote, Sara; Hernández Rodríguez, Medardo Vicente; Recio Visedo, María Paz; Climent Flórez, Belén; Agis Torres, Ángel; Raposo González, Rafaela; Contreras Jimenez, Cristina; Sánchez Pina, Ana Alejandra; Hernándes Martín, Marina; Rodríguez Prado, Claudia; Muñoz Picos, Mercedes; Perales Calvo, Manuel; Puente Maya, Francisco Jesús; Bragado Aguado, María del Carmen
  • Publication
    Implementación del aprendizaje basado en problemas (ABP) en la enseñanza práctica de la fisiología del sistema digestivo
    (2019-06-22) López-Oliva Muñoz, María Elvira; Sánchez Pina, Ana Alejandra; Hernández Rodríguez, Medardo Vicente; Prieto Ocejo, Dolores; García Sacristán, Albino; Contreras Jiménez, Cristina; Martínez Gómez, Ana Cristina; Agis Torres, Angel; Climent Florez, Belén; Rivera de los Arcos, Luis; Recio Visedo, María Paz; Benedito Castellote, Sara; Muñoz Picos, Mercedes; Raposo González, Rafaela
  • Publication
    Autoevaluación, Coevaluación y el uso de las TIC como enfoque innovador en las prácticas de Fisiopatología y su efecto en el proceso de enseñanza-aprendizaje del alumno
    (2023-07-31) Leite Fernandes, Vitor Samuel; Agis Torres, Ángel; Benedito Castellote, Sara; Climent Flórez, Belén; Contreras Jiménez, Cristina; García Sacristán, Albino; Gómez del Val, Alfonso; Hernández Rodríguez, Medardo Vicente; Hernández Martín, Marina; López-Oliva Muñoz, María Elvira; Merino Martín, José Joaquín; Montenegro Álvarez De Tejera, María Pilar; Muñoz Picos, Mercedes; Navarro Dorado, Jorge; Pascual Gómez, Natalia Fernanda; Perales Calvo, Manuel; Prieto Ocejo, Dolores; Puente Maya, Francisco Jesus; Raposo González, Rafaela; Recio Visedo, María Paz; Rivera De Los Arcos, Luis; Sánchez Pina, Ana Alejandra
    En las últimas décadas, la educación universitaria ha evolucionado hacia un enfoque constructivista en consonancia con las recomendaciones del Espacio Europeo de Educación Superior (EEES). En este paradigma, los estudiantes asumen un papel activo en el proceso de enseñanza-aprendizaje, mientras los profesores actúan como facilitadores. Las metodologías constructivistas fomentan el desarrollo tanto individual como grupal de competencias específicas y genéricas, al tiempo que permiten la inclusión de agentes de evaluación formativa para estimular la crítica y la autocrítica del alumno en su desempeño. En este contexto, surge la necesidad de aplicar el constructivismo a la evaluación, involucrando al estudiante en su propio proceso de evaluación. La autoevaluación y la coevaluación emergen como alternativas concretas para lograrlo. La autoevaluación implica que el estudiante analice y valore de manera sistemática su trabajo durante el proceso de aprendizaje para mejorar resultados y fomentar la autocrítica. Por otro lado, la coevaluación es una evaluación entre compañeros que permite valorar la implicación y actitud de los miembros del grupo, estimulando el aprendizaje colectivo. Las Tecnologías de la Información y Comunicación (TIC) juegan un papel importante en la educación y en la evaluación de los alumnos, diferenciándose de las prácticas tradicionales. La implementación de TIC no solo desarrolla habilidades en el proceso enseñanza-aprendizaje, sino también favorece la autoevaluación y la coevaluación. Con base en este enfoque, se presenta un proyecto de innovación docente en la asignatura de Fisiopatología para estudiantes de Farmacia. Los alumnos crearán videos sobre temas específicos de la práctica y se evaluarán a sí mismos y a sus compañeros utilizando la herramienta App Plickers. Sin embargo, aún no existe una metodología claramente definida para la implementación de estrategias constructivistas y uso de TIC en Fisiopatología, destacando la importancia y relevancia de este proyecto.
  • Publication
    Aprendizaje basado en problemas (ABP) mediante simulación en la enseñanza práctica de la Fisiopatología del sistema nervioso
    (2017-06-28) Martinez Gómez, Ana Cristina; Climent Florez, Belén; Recio Visedo, María Paz; Benedito Castellote, Sara; Agis Torres, Ángel; López-Oliva Muñoz, María Elvira; Muñoz Picos, Mercedes; García Sacristán, Albino; Rivera de los Arcos, Luis; Prieto Ocejo, Dolores; Hernández Rodríguez, Medardo Vicente; Sánchez Pina, Ana Alejandra
  • Publication
    Urolithiasis Develops Endothelial Dysfunction as a Clinical Feature.
    (MDPI, 2021-05-04) Sáenz Medina, Javier; Martinez, María; Rosado, Silvia; Durán Poveda, Manuel; Prieto Ocejo, Dolores; Carballido, Joaquín Alberto
    An increased risk of cardiovascular morbidity has been reported in lithiasic patients. In this context, endothelial dysfunction (ED), an earlier status of atherogenesis, has been identified in hyperoxaluria rat models of urolithiasis. Objective: The purpose of this study was to determine the endothelial vascular function in patients with urolithiasis in relation to systemic inflammatory, oxidative stress, and vascular function serum markers. Methods: A cross-sectional study was performed between 27 urolithiasic patients, matched for age and sex, with 27 healthy patients. Endothelial function was assessed by measuring flow-mediated dilation (Celermajer method). Fasting blood was collected to determine metabolic parameters (glucose and lipid profile), along with serum CRP, IL-6, MDA, ADMA, and VCAM-1. Results: Both the control and urolithiasis groups were homogenous in anthropometric, exploration, and general laboratory measures. Flow-mediated dilation (%FMD) was 11.85% (SE: 2.78) lower in the lithiasis group (p < 0.001). No significant differences were achieved between groups when CRP, IL-6, MDA, ADMA, and VCAM-1 were compared, although slightly higher values of CRP, ADMA, and VCAM-1 were detected in the lithiasic group. A correlation was not reached in any of the serum markers when they were related to flow-mediated values, although a slight negative correlation trend was observed in MDA, VCAM-1, and IL-6 values. Conclusions: Endothelial dysfunction constitutes an important disorder related to urolithiasis patients. It must be considered as an early feature responsible for future cardiovascular events. Our study did not find a significant association between inflammatory, oxidative stress, endothelial serum markers, and flow-mediated dilation.
  • Publication
    Differential contribution of renal cytochrome P450 enzymes to kidney endothelial dysfunction and vascular oxidative stress in obesity
    (Elsevier, 2021-11-22) Muñoz Picos, Mercedes; López-Oliva Muñoz, María Elvira; Pinilla Pérez, Estéfano; Rodríguez, Claudia; Martínez Saiz, María Pilar; Contreras Jiménez, Cristina; Gómez, Alfonso; Benedito Castellote, Sara; Sáenz Medina, Javier; Rivera de los Arcos, Luis; Prieto Ocejo, Dolores
    Arachidonic acid (AA)-derived cytochrome P450 (CYP) derivatives, epoxyeicosatrienoic acids (EETs) and 20-hidroxyeicosatetranoic acid (20-HETE), play a key role in kidney tubular and vascular functions and blood pressure. Altered metabolism of CYP epoxygenases and CYP hydroxylases has differentially been involved in the pathogenesis of metabolic disease-associated vascular complications, although the mechanisms responsible for the vascular injury are unclear. The present study aimed to assess whether obesity-induced changes in CYP enzymes may contribute to oxidative stress and endothelial dysfunction in kidney preglomerular arteries. Endothelial function and reactive oxygen species (ROS) production were assessed in interlobar arteries of obese Zucker rats (OZR) and their lean counterparts lean Zucker rats (LZR) and the effects of CYP2C and CYP4A inhibitors sulfaphenazole and HET0016, respectively, were examined on the endothelium-dependent relaxations and O2 − and H2O2 levels of preglomerular arteries. Non-nitric oxide (NO) non-prostanoid endothelium-derived hyperpolarization (EDH)-type responses were preserved but resistant to the CYP epoxygenase blocker sulfaphenazole in OZR in contrast to those in LZR. Sulfaphenazole did not further inhibit reduced arterial H2O2 levels, and CYP2C11/CYP2C23 enzymes were downregulated in intrarenal arteries from OZR. Renal EDH-mediated relaxations were preserved in obese rats by the enhanced activity and expression of endothelial calcium-activated potassium channels (KCa). CYP4A blockade restored impaired NO-mediated dilatation and inhibited augmented O2 − production in kidney arteries from OZR. The current data demonstrate that both decreased endothelial CYP2C11/ CYP2C23-derived vasodilator H2O2 and augmented CYP4A-derived 20-HETE contribute to endothelial dysfunction and vascular oxidative stress in obesity. CYP4A inhibitors ameliorate arterial oxidative stress and restore endothelial function which suggests its therapeutic potential for the vascular complications of obesity-associated kidney injury.
  • Publication
    Activation of AMP kinase ameliorates kidney vascular dysfunction, oxidative stress and inflammation in rodent models of obesity
    (Wiley, 2021-06-30) Rodríguez Prados, Claudia; Sánchez Pina, Ana Alejandra; Sáenz Medina, Javier; Muñoz Picos, Mercedes; Hernández Rodríguez, Medardo Vicente; López, Miguel; Rivera de los Arcos, Luis; Contreras Jiménez, Cristina; Prieto Ocejo, Dolores
    Background and Purpose Obesity is a risk factor for the development of chronic kidney disease independent of diabetes, hypertension and other co-morbidities. Obesity-associated nephropathy is linked to dysregulation of the cell energy sensor AMP-activated protein kinase (AMPK). We aimed here to assess whether impairment of AMPK activity may cause renal arterial dysfunction in obesity and to evaluate the therapeutic potential of activating renal AMPK. Experimental Approach Effects of the AMPK activator A769662 were assessed on intrarenal arteries isolated from ob/ob mice and obese Zucker rats and then mounted in microvascular myographs. Superoxide and hydrogen peroxide production were measured by chemiluminescence and fluorescence, respectively, and protein expression was analysed by western blotting. Key Results Endothelium-dependent vasodilation and PI3K/Akt/eNOS pathway were impaired in preglomerular arteries from genetically obese rats and mice, along with impaired arterial AMPK activity and blunted relaxations induced by the AMPK activator A769662. Acute ex vivo exposure to A769662 restored endothelial function and enhanced activity of PI3K/Akt/eNOS pathway in obese rats, whereas in vivo treatment with A769662 improved metabolic state and ameliorated endothelial dysfunction, reduced inflammatory markers and vascular oxidative stress in renal arteries and restored redox balance in renal cortex of obese mice. Conclusion and Implications These results demonstrate that AMPK dysregulation underlies obesity-associated kidney vascular dysfunction and activation of AMPK improves metabolic state, protects renal endothelial function and exerts potent vascular antioxidant and anti-inflammatory effects. The beneficial effects of vascular AMPK activation might represent a promising therapeutic approach to the treatment of obesity-related kidney injury.
  • Publication
    Genetic Targeting of GRP78 in the VMH Improves Obesity Independently of Food Intake
    (MDPI, 2018-07-17) Liñares-Pose, Laura; Rial-Pensado, Eva; Estévez-Salguero, Ánxela; Milbank, Edward; González-García, Ismael; Rodríguez Prados, Claudia; Seoane-Collazo, Patricia; Martinez-Sánchez, Noelia; Nogueiras, Rubén; Prieto Ocejo, Dolores; Diéguez, Carlos; Contreras Jiménez, Cristina; López, Miguel
    Recent data have demonstrated that the hypothalamic GRP78/BiP (glucose regulated protein 78 kDa/binding immunoglobulin protein) modulates brown adipose tissue (BAT) thermogenesis by acting downstream on AMP-activated protein kinase (AMPK). Herein, we aimed to investigate whether genetic over-expression of GRP78 in the ventromedial nucleus of the hypothalamus (VMH: a key site regulating thermogenesis) could ameliorate very high fat diet (vHFD)-induced obesity. Our data showed that stereotaxic treatment with adenoviruses harboring GRP78 in the VMH reduced hypothalamic endoplasmic reticulum ER stress and reversed vHFD-induced obesity. Herein, we also demonstrated that this body weight decrease was more likely associated with an increased BAT thermogenesis and browning of white adipose tissue (WAT) than to anorexia. Overall, these results indicate that the modulation of GRP78 in the VMH may be a target against obesity.
  • Publication
    Implementación de un sistema b-learning en la enseñanza práctica de Fisiopatología
    (2016-11) Hernández Rodríguez, Medardo Vicente; Sánchez Pina, Ana Alejandra; Agis-Torres, Ángel; Recio Visedo, María Paz; Benedito Castellote, Sara; Raposo González, Rafaela; Muñoz-Picos, Mercedes; López-Oliva, Elvira; Garcia-Sacristan, Albino; Prieto Ocejo, Dolores; Rivera de los Arcos, Luis; Martínez Gómez, Ana Cristina; Climent Florez, Belén
  • Publication
    Phosphodiesterase type 4 inhibition enhances nitric oxide- and hydrogen sulfide-mediated bladder neck inhibitory neurotransmission
    (Nature Research, 2018-03-16) Agis Torres, Ángel; Recio Visedo, María Paz; López-Oliva Muñoz, María Elvira; Martínez Sainz, María Del Pilar; Barahona Gomáriz, María Victoria; Benedito Castellote, Sara; Bustamante, Salvador; Jiménez-Cidre; Miguel Ángel; García Sacristán, Albino; Prieto Ocejo, Dolores; Leite Fernandes, Vitor Samuel; Hernández Rodríguez, Medardo Vicente
    Nitric oxide (NO) and hydrogen sulfide (H2S) play a pivotal role in nerve-mediated relaxation of the bladder outflow region. In the bladder neck, a marked phosphodiesterase type 4 (PDE4) expression has also been described and PDE4 inhibitors, as rolipram, produce smooth muscle relaxation. This study investigates the role of PDE4 isoenzyme in bladder neck gaseous inhibitory neurotransmission. We used Western blot and double immunohistochemical staining for the detection of NPP4 (PDE4) and PDE4A and organ baths for isometric force recording to roflumilast and tadalafil, PDE4 and PDE5, respectively, inhibitors in pig and human samples. Endogenous H2S production measurement and electrical field stimulation (EFS) were also performed. A rich PDE4 and PDE4A expression was observed mainly limited to nerve fibers of the smooth muscle layer of both species. Moreover, roflumilast produced a much more potent smooth muscle relaxation than that induced by tadalafil. In porcine samples, H2S generation was diminished by H2S and NO synthase inhibition and augmented by roflumilast. Relaxations elicited by EFS were potentiated by roflumilast. These results suggest that PDE4, mainly PDE4A, is mostly located within nerve fibers of the pig and human bladder neck, where roflumilast produces a powerful smooth muscle relaxation. In pig, the fact that roflumilast increases endogenous H2S production and EFS-induced relaxations suggests a modulation of PDE4 on NO- and H2S-mediated inhibitory neurotransmission.