Person: Sánchez Calabuig, María Jesús
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First Name
María Jesús
Last Name
Sánchez Calabuig
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Medicina y Cirugía Animal
Area
Medicina y Cirugía Animal
Identifiers
13 results
Search Results
Now showing 1 - 10 of 13
Item Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures(Animal Rerpoduction Science, 2020) Consuegra González, Cesar; Crespo Castejón, Francisco; Dorado Martín, Jesús; María Díaz Jiménez; Sánchez Calabuig, María Jesús; Beltrán Breña, Paula; Pérez Cerezales, Serafín; Rizos, Dimitri; Hidalgo PrietoThe aim of this study was to evaluate the fertilizing capacity of frozen or vitrified stallion sperm after assessing different warming procedures. In Experiment 1, different warming procedures were compared after sperm vitrification: immersion in extender at 43 ◦C (C), or in a water bath at 37 ◦C/30 s (W37), 43 ◦C/10 s (W43) or 60 ◦C/5 s (W60). With the W60 treatment, there were greater values (P < 0.05) for VCL (83.93 ± 3.6 μm/s) and ALH (3.00 ± 0.2 μm) than freezing and with the C group, and greater values (P < 0.001) for PM (35.33 ± 2.5 %) than with the W43 treatment. In Experiment 2, the fertilizing capacity of vitrified and frozen sperm was assessed utilizing heterologous IVF procedures, using cattle oocytes. Vitrification resulted in greater values (P < 0.05) than freezing for the number of bound sperm (1.36 ± 0.3 and 0.69 ± 0.2, respectively). There were no differences between frozen or vitrified sperm in pronuclear formation (26 hours post-insemination - hpi; 14.08 ± 4.2 % and 22.78 ± 4.8 %, respectively) or cleavage rate (32.77 ± 4.3 % and 39.66 ± 4.6 %, respectively). In conclusion, vitrified stallion sperm warmed in a water bath at 60 ºC had the capacity to penetrate cattle oocytes, leading to pronuclear formation and hybrid embryo cleavage after heterologous IVF.- Project number: 193
Item Elaboración de material docente para la enseñanza de la ecografía pulmonar en el bovino(2022) Re, Michela Tatiana; Villaescusa Fernández, Alejandra; Sánchez Calabuig, María Jesús; Coronel Araujo, Carlos Críspulo; Romero Crespo, Isabel; Mejías López, Elena; Blanco Murcia, Francisco JavierCon este proyecto se quiere desarrollar material audiovisual que sirva de apoyo a las prácticas que reciben los alumnos de Veterinaria. Dicho material incluirá descripciones de la técnica ecográfica pulmonar y de la interpretación de las imágenes, incluyendo material visual de tipo multimedia sobre la ecografía del aparato respiratorio fisiológico y patológico en el bovino. Con este proyecto se pretende facilitar el acceso a material docente útil para el aprendizaje de la ecografía, de forma que los estudiantes y los profesionales veterinarios puedan afianzar sus conocimientos de dicha técnica de forma previa a su empleo en los animales vivos. Item Desarrollo de métodos alternativos de valoración y criopreservación seminal en el delfín mular ("Tursiops truncatus")(2015) Sánchez Calabuig, María Jesús; Fuente Martínez, Julio de la; Pérez Gutiérrez, José FélixItem A high glucose concentration during early stages of in vitro equine embryo development alters expression of genes involved in glucose metabolism(Equine Veterinary Journal, 2020) Sánchez Calabuig, María Jesús; Fernández-González, Raul; Hamdi, Meriem; Smits, Katrien; López Cardona, Ángela Patricia; Serres Dalmau, María Consolacion; Macías García, Beatriz; Gutiérrez Adán, AlfonsoBackground: Equine embryos exhibit an unusual pattern of glucose tolerance in vitro and are currently cultured in hyperglycaemic conditions. Objective: Our main objective was to analyse the effect of different glucose concentrations on in vitro-produced equine embryo development and quality. Study design: Experiments comparing in vitro and in vivo produced embryos. Methods: Oocytes (n = 641) were collected from post-mortem ovaries, matured in vitro and fertilised by intracytoplasmic sperm injection (ICSI). Embryo culture was divided from Day 0 to Day 4 and from Day 4 to Day 9 in three groups: 5-10 (5 and 10 mmol/L glucose respectively; n = 87); 5-17 (5 and 17.5 mmol/L; n = 66); and 10-17 (10 and 17.5 mmol/L; n = 117). A control group of 20 in vivo produced blastocysts was included. Cleavage and blastocyst rates were evaluated and embryos were snap-frozen for analysis of the relative mRNA expression of genes related to mitochondrial function, DNA methylation, apoptosis, glucose transport and metabolism. Results: No differences were observed in the cleavage or blastocyst rates among in vitro groups. Under high glucose conditions in vitro (10-17 group), BAX/BCL2 was higher, and PFKP, LDHA and COX2 were overexpressed compared to all other groups. The two groups with 5 mmol/L glucose concentration during the first culture stage (5-10 and 5-17) displayed similar patterns which differed to the 10-17 group. Main limitations: Conclusions related to embryo quality are based on gene expression patterns. Transfer of in vitro-produced embryos would reveal whether the observed differences improve embryo developmental competence. Conclusions: Five mM glucose during the first days of culture seems to be preferable to avoid over-activation of embryonic glycolytic pathways. Further studies are necessary to determine whether this improves embryo developmental competence.- Project number: 36
Item Modelo docente de palpación del aparato reproductor en bovino como alternativa al uso de materiales de prácticasblanco(2023) Sánchez Calabuig, María Jesús; Blanco Murcia, Francisco Javier; Moreno Gonzalo, Javier; Fominaya García, Hernán Luis; Zanitoni, Morgane; Serres Dalmau, María Consolacion; Crespo Castejón, Francisco; Domínguez Gimbernat, Mónica; Gutiérrez Cepeda, Luna; Mejías López, Elena; Sánchez Calabuig, María Jesús - Project number: 165
Item Guía multimedia de producción y vitrificación de embriones en la especie bovina(2019) Sánchez Calabuig, María Jesús; Blanco Murcia, Francisco Javier; Pérez Gutiérrez, Jose Felix; Coronel Araujo, Carlos Críspulo; Herrero García, VictorLa aplicación de las nuevas biotecnologías de la reproducción en las explotaciones ganaderas produce un aumento en la producción, así como una mejora genética rápida y notable. Por ello, estas técnicas tienen gran relevancia y son de estudio obligado en el grado de Veterinaria. Su evolución es muy dinámica y mantener los conocimientos actualizados es complicado. El diseño de nuevas herramientas que adecuen la metodología de enseñanza a las nuevas tecnologías es un modo de facilitar estos conocimientos a las nuevas generaciones de estudiantes. En el presente proyecto, se ha realizado una guía multimedia de producción y vitrificación de embriones centrada en la especie bovina. Dicha guía interactiva incluye videos de los procedimientos (aplicación de fármacos, inseminación artificial, recuperación de embriones, evaluación de la calidad embrionaria, vitrificación y transferencia de embriones a las hembras receptoras) así como documentos que describen los protocolos para cada uno de los procedimientos. Todos estas herramientas están subidos en una página web creada para el proyecto www.reprovets.org. Item Postnatal Catch-Up Growth Programs Telomere Dynamics and Glucose Intolerance in Low Birth Weight Mice(International Journal of Molecular Sciences, 2021) Pericuesta, Eva; Gutiérrez Arroyo, Julia L.; Sánchez Calabuig, María Jesús; Gutiérrez Adán, AlfonsoLow birth weight and rapid postnatal weight gain are independent predictors of obesity and diabetes in adult life, yet the molecular events involved in this process remain unknown. In inbred and outbred mice, this study examines natural intrauterine growth restriction (IUGR) in relation to body weight, telomere length (TL), glucose tolerance, and growth factor gene (Igf1, Igf2, Insr, Igf1r, and Igf2r) mRNA expression levels in the brain, liver, and muscle at 2- and 10 days of age and then at 3- and 9 months of age. At birth, ~15% of the animals showed IUGR, but by 3 and 9 months, half of these animals had regained the same weight as controls without IUGR (recuperated group). At 10 days, there was no difference in TL between animals undergoing IUGR and controls. However, by 3 and 9 months of age, the recuperated animals had shorter TL than the control and IUGR-non recuperated animals and also showed glucose intolerance. Further, compared to controls, Igf1 and Igf2 growth factor mRNA expression was lower in Day 2-IUGR mice, while Igf2r and Insr mRNA expression was higher in D10-IUGR animals. Moreover, at 3 months of age, only in the recuperated group were brain and liver Igf1, Igf2, Insr, and Igf2r expression levels higher than in the control and IUGR-non-recuperated groups. These data indicate that catch-up growth but not IUGR per se affects TL and glucose tolerance, and suggest a role in this latter process of insulin/insulin-like growth signaling pathway gene expression during early development.Item Resveratrol–cyclodextrin complex affects the expression of genes associated with lipid metabolism in bovine in vitro produced embryos(Reproduction in Domestic Animals, 2018) Torres, Viviana; Hamdi, Meriem; Millán de la Blanca, María Gema; Urrego, R.; Echeverri, J.; López Herrera, A.; Rizos, Dimitri; Gutiérrez Adán, Alfonso; Sánchez Calabuig, María JesúsAntioxidants have been widely used during in vitro production to decrease the negative effect of reactive oxygen species. It was reported that the complex resveratrol-methyl β-cyclodextrin (RV-CD) improves resveratrol's stability and bioavailability and increases its antioxidant activity. This study evaluates the effect of RV-CD during in vitro oocyte maturation (IVM) or in vitro embryo culture (IVC) on developmental competence and quantitative changes in gene expression of developmental important genes. In experiment 1, RV-CD was added to IVM media and maturation level, embryo development and oocytes, cumulus cells, and blastocysts gene expression by RT-qPCR were examined. In experiment 2, presumptive zygotes were cultured in SOF supplemented with RV-CD and embryo development and blastocysts gene expression by RT-qPCR were studied. A group without RV-CD (control- ) and a group with cyclodextrin (control+ ) were included. No differences were found in cleavage rate or blastocyst yield between groups. However, the expression of LIPE was higher in blastocysts derived from oocytes treated with resveratrol compared with control groups (p < .05). Blastocysts produced by IVC with resveratrol showed that RV-CD could modify the expression of genes related to lipid metabolism (CYP51A1, PNPLA2 and MTORC1) compared with control groups (p < .05). RV-CD in the IVM and IVC media could reduce accumulated fat by increasing lipolysis and suppressing lipogenesis of blastocysts.Item Expanded equine cumulus–oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus–oocyte complexes(Reproduction, Fertility and Development, 2017) González-Fernández, Lauro; Sánchez Calabuig, María Jesús; Alves, M. G.; Oliveira, Pedro F.; Macedo, S.; Gutiérrez Adán, Alfonso; Rocha, A.; Macías García, BeatrizEquine cumulus-oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This difference could be related to divergent glucose metabolism. To test this hypothesis in the present study, eCOCs, cCOCs and expanded or compact mural granulosa cells (EC and CC respectively) were matured in vitro for 30h, at which time maturation rate, glucose metabolism and the expression of genes involved in glucose transport, glycolysis, apoptosis and meiotic competence were determined. There were significant differences between eCOCs and cCOCs in maturation rate (50% vs 21.7% (n=192 and 46) respectively; P<0.001), as well as mean (±s.e.m.) glucose consumption (1.8±0.5 vs 27.9±5.9 nmol per COC respectively) and pyruvate (0.09±0.01 vs 2.4±0.8 nmol per COC respectively) and lactate (4.7±1.3 vs 64.1±20.6 nmol per COC respectively; P<0.05 for all) production. Glucose consumption in EC and CC did not differ significantly. Expression of hyaluronan-binding protein (tumour necrosis factor alpha induced protein 6; TNFAIP6) was increased in eCOCs and EC, and solute carrier family 2 member 1 (SLC2A1) expression was increased in eCOCs, but there were no differences in the expression of glycolysis-related enzymes and solute carrier family 2 member 3 (SLC2A3) between the COC or mural granulosa cell types. The findings of the present study demonstrate that metabolic and genomic differences exist between eCOCs and cCOCs and mural granulosa cells in the horse.Item Reproductive Cycle Stage Assessment in the Two-Toed Sloth (Choloepus Hoffmanni, Preliminary Results(54, 2024) Calvo Fernández, Cristina; Such, Roger; Gutiérrez Cepeda, Luna; Gómez Redondo, Isabel; García Vila, Encarnación; Cerdeira Lozano, Joaquín Vicente; Mayenco Aguirre, Ana María; Santiago Moreno, Julián; Hernández, Leticia; Sánchez Calabuig, María JesúsThe number of two-toed sloths (Choloepus hoffmanni) has significantly decreased in the last years. Deepening the knowledge of this tropical mammal's reproductive physiology is essential to improve captive breeding within conservation programs for this species. However, several aspects of its reproductive biology remain unexplored and have not been described sufficiently. The aim of this work was to describe the estrous cycle and reproductive physiology of two adult female C. hoffmanni by vaginal cytology, appearance of the external genitalia, and behavior. Vaginal cytology assay showed that the average duration of the estrous cycle was 15.1 ± 4.53 d. Positive correlations (P < 0.05) were found between the peak presence of superficial cells (estrous phase) and four parameters: aggressive behavior, pursuing behavior, vulvar swelling, and vaginal discharge. This pilot study, conducted on just two animals, forms a basis for a study design that may be employed for a more comprehensive assessment of the two-toed sloth reproductive physiology and behavior.