Person: López Ruiz, María Beatriz
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First Name
María Beatriz
Last Name
López Ruiz
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Analítica
Identifiers
17 results
Search Results
Now showing 1 - 10 of 17
Item Electrochemical genosensor for Klotho detection based on aliphatic and aromatic thiols self-assembled monolayers(Talanta, 2020) Sánchez-Paniagua López, Marta; Palenzuela Batista, Sara; Manzanares Palenzuela, Lorena; López Ruiz, María BeatrizChanges in expression of Klotho gene are associated with chronic kidney disease and its potential as early biomarker is being studied. We report, for the first time, the detection of Klotho gene by a biosensor platform. Self-assembled mixed monolayers (SAMs) as DNA immobilization method in screen-printed gold electrodes and a sandwich format detection were used in the development of an electrochemical genosensor for the detection of a 100-mer DNA fragment, copy of the partial region of the mRNA Klotho gene. The use of different binary and ternary SAMs based on aliphatic (mercaptohexanol, MCH, and hexanedithiol, HDT) and aromatic (mercaptophenylacetic acid, MPAA) thiol diluents and capture probe (CP) as sensing phases was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. Multiple configurations were studied, changing the order of component addition and comparing co-immobilization and two-step immobilization processes. The procedure for binary SAM preparation consisting of sequential addition of a thiol diluent followed by CP was found to have the least detrimental impact on electrochemical performance. The signal-to-blank ratios increased considerably in the case of thioaromatic binary DNA monolayers, MPPA/CP, compared to the values obtained for aliphatic SAMs. Ternary monolayers formed by MCH and HDT rendered good fractional coverage levels and generated more reversible redox reactions at the surface, mostly when CP was firstly immobilized, CP/HDT/MCH. A significant reduction of the blank and non-specific (non-complementary sequence) signals was obtained with this ternary SAM, compared to binary SAMs and an increase of 2.42-fold of the S/B ratio (10 nM of target) compared with MPAA/CP SAMs. A linear response in the range of 5·10−10 to 5·10−8 M was obtained with CP/HDT/MCH monolayer, with a detection limit of 0.5 nM and RSD of 8.10%.- Project number: 185
Item ¿Y si nos escapamos de manera virtual? Hacia la virtualización de las "escape rooms"(2021) Mateos-Aparicio Cediel, Inmaculada; Sánchez-Paniagua López, Marta; López Ruiz, María Beatriz; Rueda Rodríguez, María del Carmen; García Alonso, María Alejandra; Molina Santos, Marina Mercedes; Moreno Guzmán, María; Pérez Rodríguez, María Luisa; Raggio Quilez, José; Sevilla Sierra, María Paz; González Aguilera, Guillermo; Gonzalez de Toro, Teresa Almudena; Jiménez de la Peña, Rocío Teresa Item Edible plant by-products as source of polyphenols: prebiotic effect and analytical methods(Critical Reviews in Food Science and Nutrition, 2022) García Alonso, María Alejandra; Sánchez-Paniagua López, Marta; Manzanares-Palenzuela, C. Lorena; Redondo Cuenca, Araceli; López Ruiz, María BeatrizPolyphenols with high chemical diversity are present in vegetables both in the edible parts and by-products. A large proportion of them remains unabsorbed along the gastrointestinal tract, being accumulated in the colon, where they are metabolized by the intestinal microbiota. These polyphenols have been found to have “prebiotic-like” effects. The edible plant industry generates tons of residues called by-products, which consist of unutilized plant tissues (peels, husks, calyxes and seeds). Their disposal requires special and costly treatments to avoid environmental complications. Reintroducing these by-products into the value chain using technological and biotechnological practices is highly appealing since many of them contain nutrients and bioactive compounds, such as polyphenols, with many health-promoting properties. Edible plant by-products as a source of polyphenols highlights the need for analytical methods. Analytical methods are becoming increasingly selective, sensitive and precise, but the great breakthrough lies in the pretreatment of the sample and in particular in the extraction methods. This review shows the importance of edible plant by-products as a source of polyphenols, due to their prebiotic effect, and to compile the most appropriate analytical methods for the determination of the total content of phenolic compounds as well as the detection and quantification of individual polyphenols.Item Biosensors for GMO Testing: Nearly 25 Years of Research(2018) Sánchez-Paniagua López, Marta; Manzanares Palenzuela, Carmen Lorena ; López Ruiz, María BeatrizIn the nearly two decades since genetically modified organisms (GMOs) were first commercialized, genetically engineered crops have gained ground on their conventional counterparts, reaching 185 million hectares worldwide in 2016. The technology has bestowed most of its benefits on enhancing crop productivity with two main traits currently dominating the market: insect-resistant and herbicide-tolerant crops. Despite their rapid and vast adoption by farmers worldwide, GMOs have generated heated debates, especially in European countries (EU), driven mostly by consumers concerned about safety of transgenic foods and about the potential impact on the environment. The need to monitor and to verify the presence and the amount of GMOs in agricultural crops and in food products has generated interest in analytical methods for sensitive, accurate, rapid, and cheap detection of these products. DNA biosensors have been envisioned as a novel DNA-detection technology that would one day substitute current amplification-based methods, providing hand-held, quick, and ultrasensitive gene-level detection. This review summarizes the contributions made in nearly 20 years of research regarding the application of genosensing technology for the qualitative and quantitative determination of transgenic traits.Item Synthesis, characterization and use of enzyme cashew gum nanoparticles for biosensing applications(Journal of Materials Chemistry B, 2021) Amorim, Adriany das Graças Nascimento; Sánchez-Paniagua López, Marta; Oliveira, Taiane Maria de; Mafud, Ana Carolina; Alves da Silva, Durcilene; Souza de Almeida Leite, José Roberto de; López Ruiz, María BeatrizThis research reports, for the first time, the immobilization of an enzyme - Rhus vernificera laccase - on cashew gum (CG) nanoparticles (NPs) and its application as a biological layer in the design and development of an electrochemical biosensor. Laccase-CG nanoparticles (LacCG-NPs) were prepared by the nanoprecipitation method and characterized by UV-Vis spectrophotometry, atomic force microscopy, scanning electron microscopy, attenuated total reflectance-Fourier-transform infrared spectroscopy, circular dichroism, cyclic voltammetry, and electrochemical impedance spectroscopy. The average size and stability of the NPs were predicted by DLS and zeta potential. The ATR-FTIR results clearly demonstrated an interaction between –NH and –OH groups to form LacCG-NPs. The average size found for LacCG-NPs was 280 53 nm and a polydispersity index of 0.309 0.08 indicated a good particle size distribution. The zeta potential shows a good colloidal stability. The use of a natural product to prepare the enzymatic nanoparticles, its easy synthesis and the immobilization efficiency should be highlighted. LacCG-NPs were successfully applied as a biolayer in the development of an amperometric biosensor for catechol detection. The resulting device showed a low response time (6 s), good sensitivity (7.86 mA mM1 cm2 ), wide linear range of 2.5 107 –2.0 104 M, and low detection limit (50 nM).- Project number: 118
Item "Escape Seminar": Escápate del seminario(2020) Mateos-Aparicio Cediel, Inmaculada; Sánchez-Paniagua López, Marta; García Alonso, María Alejandra; López Ruiz, María Beatriz; Molina Santos, Marina Mercedes; Moreno Guzmán, María; Raggio Quilez, José; Rueda, María del Carmen; Sevilla Sierra, María Paz; Bodas Folguera, Cristina; Jimenez de la Peña Armada, Rocio TeresaLas "escape room" son juegos de equipo para "escapar" de una sala o una sala virtual. Esta sala está llena de desafíos que se deben superar para ganar (escapar) dentro de un límite de tiempo determinado. Debido a que la enseñanza tradicional está causando problemas en torno a la motivación y el compromiso, y muchos estudiantes la perciben como ineficaz y aburrida, el uso de juegos educativos es muy prometedor porque son una herramienta de enseñanza para mejorar las habilidades no cognitivas, como la resolución de problemas y la colaboración. y comunicación. Los juegos tienen un notable poder motivador debido al uso de una serie de mecanismos que alientan a las personas a participar en ellos, a menudo sin ninguna recompensa, solo por la satisfacción de jugar y la posibilidad de ganar. En el curso 2019-2020, hemos desarrollado actividades basadas en el concepto de la "escape room" en las asignaturas: "Ciencia y Análisis de agua y bebidas" del Grado en Ciencia y Tecnología de Alimentos, "Química Analítica I" y "Físico-Química Farmacéutica" del Grado de Farmacia y “Química” del Grado en Óptica y Optometría de la Universidad Complutense de Madrid. Para el desarrollo del proyecto, todas las características y el progreso de las diferentes fases del seminario de la "escape room" se informaron en el primer día de clase y a través de la plataforma del Campus Virtual. Los estudiantes se agruparon (5-7 personas / grupo) y nombraron un portavoz que fue responsable de responder los retos de la actividad. Al final de esta acción, los estudiantes respondieron voluntariamente a las encuestas Likert para evaluar sus implicaciones y su percepción sobre su aprendizaje, trabajo en equipo y si les gustó la actividad propuesta. - Project number: 84
Item Elaboración de videos tutoriales: resolver exámenes no es difícil en el Grado en Farmacia y en el Grado en Óptica y Optometría (Física,Fisicoquímica, Química Analítica y Química)(2017) Sevilla Sierra, María Paz; Rueda Rodríguez, María Del Carmen; Galera Gómez, Pedro Antonio; Sainz Villanueva, María Cristina; López Cabarcos, Enrique; López Ruiz, María Beatriz; Sánchez-Paniagua López, Marta; Molina Santos, Marina Mercedes; Urquia Fermosell, Carlos; Manzanares Palenzuela, Carmen Lorena; Redondo Gomez, EstherSe ha creado una herramienta multimedia compuesta por un conjunto de 31 videos con una duración total de 5 horas y 43 minutos de visualización.El primero contiene el prólogo y el índice y el resto tiene carácter académico,cada uno corresponde a un capítulo y contiene una pregunta de examen y su respuesta, así como la explicación correspondiente. Se han recogido en una publicación multimedia: "Resolver cuestiones y problemas de química y física es fácil: Grado Farmacia, Grado Óptica y Optometría" (ISBN 978-84-617-9258-0) en la que cada capítulo puede constituirse como un video independiente. Se ha creado un canal YouTube (https://www.youtube.com/channel/UC9sr_FovY4q-XqF05J3STWQ) para difundir los videos, se han presentado dos contribuciones a un Congreso Internacional y se han escrito dos proceedings. - Project number: 263
Item Diseño y aplicación de actividades virtuales. Análisis de datos de aprendizaje relacionados con la utilización de dichas actividades (learning analytics)(2015) López Ruiz, María Beatriz; Frutos Cabanillas, Gloria; Rueda Rodríguez, María Del Carmen; Sevilla Sierra, María Paz; Sainz Villanueva, María Cristina; Sánchez-Paniagua López, Marta - Project number: 60
Item Respuestas química para el estudiante de Farmacia(2016) Sevilla Sierra, María Paz; Galera Gómez, Pedro Antonio; López Ruiz, María Beatriz; Rueda Rodríguez, María Del Carmen; Sainz Villanueva, María Cristina; Sánchez-Paniagua López, Marta; Santoro Domingo, PabloEl trabajo realizado en el PIMCD 2015 nº 60, cuya memoria final se presenta, ha sido posible gracias a la financiación concedida a las autoras por la Universidad Complutense de Madrid. Se han realizado videos recogidos en 2 DVDs. La colección de videos que lo componen responde a una forma moderna y eficaz, alternativa a los métodos clásicos, de explicar al alumno del Grado en Farmacia la respuesta científica a preguntas corrientes con las que puede encontrarse en su futuro profesional o en su vida diaria, relacionadas con las asignaturas de Química Física Farmacéutica, Química Analítica o Física Aplicada a Farmacia. Cada uno de ellos constituye una entidad independiente que se puede visualizar por separado y con los que se puede interaccionar parándolos, rebobinándolos o adelantándolos en el momento que se quiera y tantas veces como sea necesario, lo que permite al estudiante adaptarlo a su propia velocidad de comprensión. Esperamos que sean útiles a una gran cantidad de jóvenes universitarios y les anime a concebir estas asignaturas, no como compartimentos estancos, sino como las herramientas cuyo conocimiento y comprensión les abre las puertas de un mundo lleno de respuestas. Item Fast and sensitive diagnosis of autoimmune disorders through amperometric biosensing of serum anti-dsDNA autoantibodies(Biosensors & Bioelectronics, 2020) López Ruiz, María Beatriz; Arévalo Pérez, Beatriz; Serafín González-Carrato, Verónica; Sánchez-Paniagua López, Marta; Montero Calle, Ana; Barderas Manchado, Rodrigo; Campuzano Ruiz, Susana; Yáñez-Sedeño Orive, Paloma; Pingarrón Carrazón, José ManuelThis work reports the first amperometric biosensor involving the use of neutravidin-functionalized magnetic microbeads (NA-MBs) modified with a biotinylated-anti-dsDNA (b-dsDNA) as efficient magnetic microcarriers to selectively capture anti-dsDNA autoantibodies (IgG, IgA and IgM AAbs) present in the sera of patients with rheumatoid arthritis (RA). Subsequently, the attached anti-dsDNA AAbs are detected with a mixture of conventional HRP-labeled secondary antibodies (HRP-anti-human IgG/IgM/IgA mixture). The biorecognition event is monitored by amperometric transduction using the hydroquinone (HQ)/H2O2 system upon capturing the modified MBs on the surface of screen-printed carbon electrodes (SPCEs). The developed bioplatform exhibits a linear calibration plot ranging from 1 to 200 IU mL−1 with a LOD of 0.3 IU mL−1 for anti-dsDNA AAbs standards. In addition, the biosensor allows performing the determination of the anti-dsDNA AAbs levels directly in 100-times diluted serum samples from patients diagnosed with RA and in just 75 min. The obtained results are in agreement with those provided by an ELISA kit and allow discrimination between positive and negative samples.