Person: López Ruiz, María Beatriz
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First Name
María Beatriz
Last Name
López Ruiz
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Analítica
Identifiers
3 results
Search Results
Now showing 1 - 3 of 3
Item Biosensors for GMO Testing: Nearly 25 Years of Research(2018) Sánchez-Paniagua López, Marta; Manzanares Palenzuela, Carmen Lorena ; López Ruiz, María BeatrizIn the nearly two decades since genetically modified organisms (GMOs) were first commercialized, genetically engineered crops have gained ground on their conventional counterparts, reaching 185 million hectares worldwide in 2016. The technology has bestowed most of its benefits on enhancing crop productivity with two main traits currently dominating the market: insect-resistant and herbicide-tolerant crops. Despite their rapid and vast adoption by farmers worldwide, GMOs have generated heated debates, especially in European countries (EU), driven mostly by consumers concerned about safety of transgenic foods and about the potential impact on the environment. The need to monitor and to verify the presence and the amount of GMOs in agricultural crops and in food products has generated interest in analytical methods for sensitive, accurate, rapid, and cheap detection of these products. DNA biosensors have been envisioned as a novel DNA-detection technology that would one day substitute current amplification-based methods, providing hand-held, quick, and ultrasensitive gene-level detection. This review summarizes the contributions made in nearly 20 years of research regarding the application of genosensing technology for the qualitative and quantitative determination of transgenic traits.Item Electrochemical biosensor based on ionic liquid polymeric microparticles. An analytical platform for catechol(2018) Sánchez-Paniagua López, Marta; López Ruiz, María BeatrizA catechol amperometric biosensor based on tyrosinase has been successfully developed. Tyrosinase was immobilized into microparticles prepared by polymerization of the ionic liquid 1-vinyl-3-ethyl-imidazolium bromide (ViEtIm+Br−). Different kinds of microparticles with entrapped tyrosinase based on ionic liquid were obtained from the latter, by a simple anion-exchange reaction. The synthesis of the microparticles was optimized. The analytical method based on the proposed device was also optimized in terms of pH and temperature. Optimal values were 6.5 and 25 °C, respectively. The characterization of both, microparticles and analytical device, was performed by cyclic voltammetry and electrochemical impedance spectroscopy. A linear relationship between the current signal and the catechol concentration was obtained in the range from 3.9·10−5 to 2.5·10−4 M, with a sensitivity of 17.95 mA M−1 cm−2 and a detection limit of 20 μM. The tyrosinase activity of lyophilized poly(ViEtIm+Br−) microparticles was unchanged for at least 9 months after their synthesis. The biosensor maintains 95% of the initial response 21 days after its first use. The biosensor has been applied for detecting phenol compounds in wastewater samples. The recoveries were found in the range of 91–96%.- Project number: 5
Item Estrategias metodológicas enfocadas a la mejora del aprendizaje en estudiantes con diversidad(2018) Sánchez-Paniagua López, Marta; López Ruiz, María Beatriz; Sevilla Sierra, María Paz; Rueda Rodríguez, María Del Carmen; Molina Santos, Marina Mercedes; Mateos-Aparicio Cediel, Inmaculada; Redondo Cuenca, Araceli; Tenorio Sanz, María Dolores; Rodrigo Martín, Rosa; Aragoneses Velasco, Laura; López Salvador, Alba; Cervero Frías, Verónica; Urquía Fermosell, CarlosDiseño y desarrollo de estrategias metodológicas que faciliten el aprendizaje de los estudiantes en general y que permitan a los estudiantes con diversidad funcional la integración y participación en el proceso de aprendizaje, que refuercen su inclusión y que favorezcan la adquisición de competencias.