Person:
Batanero Cremades, Eva

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First Name
Eva
Last Name
Batanero Cremades
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
Identifiers
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Search Results

Now showing 1 - 10 of 18
  • Publication
    Characterization of Profilin and Polcalcin Panallergens From Ash Pollen
    (Esmon Publicidad, 2014) Mas García, Salvador; Garrido Arandia, María; Batanero Cremades, Eva; Purohit, A.; Pauli, G.; Rodríguez García, Rosalía; Barderas Manchado, Rodrigo; Villalba Díaz, Mayte
    Background: Ash (Fraxinus excelsior) is an important source of allergenic pollen in temperate areas of Europe. Profilin and polcalcin are 2 important panallergens involved in cross-reactivity between different sources. Objective: To clone and produce Fra e 2 (profilin) and Fra e 3 (polcalcin) as recombinant proteins and evaluate their immunological properties using the natural forms obtained from ash pollen. Methods: Total RNA from ash pollen was used as a template to obtain the specific complementary DNA (cDNA) sequences of the 2 panallergens. The cDNA-encoding sequences were cloned into the pET11b expression vector and used to transform BL21 (DE3) Escherichia coli cells. Proteins were expressed, purified by chromatography, and characterized structurally by circular dichroism, mass spectrometry, and immunologically by western blot and ELISA using profilin and polcalcin polyclonal antibodies and human sera from ash pollen–sensitized patients. Results: Profilin and polcalcin amino acid sequences from ash pollen showed a high degree of identity with homologous allergens from different sources. The cDNA-encoding allergen sequences were expressed as nonfusion recombinant proteins and purified to homogeneity. Secondary structure values were similar to those obtained from other members of these families. Allergenic Properties of the recombinant allergens were observed to be equivalent to those of the natural counterparts of F excelsior pollen.Conclusions: Fra e 2 and Fra e 3 recombinant allergens might be used in clinical diagnosis to determine profilin- and polcalcin-specific IgE levels present in the sera of ash pollen–sensitized patients, thus facilitating the finding of the sensitizing source in areas with complex sensitization profiles.
  • Publication
    ¿Qué es lo que sabemos… sobre Biología?
    (2021-05) Batanero Cremades, Eva; Rodríguez Crespo, José Ignacio; Castillo Lluva, Sonia; García Álvarez, Begoña; Bueno Díaz, Cristina
    Dada la importancia del inglés y de las tecnologías móviles hoy día, los estudiantes utilizarán dichas tecnologías para reforzar el aprendizaje de Biología, y motivarlos a estudiar, mediante dos actividades: crear test Kahoot! en inglés y concursar.
  • Publication
    Pollensomes as natural vehicles for pollen allergens
    (American Association of Immunologists, 2015-07-15) Prado, Noela; De Linares, C.; Sanz, M.L.; Gamboa, P.; Villalba Díaz, Mayte; Rodríguez García, Rosalía; Batanero Cremades, Eva
    Olive (Olea europaea) pollen constitutes one of the most important allergen sources in the Mediterranean countries and some areas of the United States, South Africa, and Australia. Recently, we provided evidence that olive pollen releases nanovesicles of respirable size, named generically pollensomes, during in vitro germination. Olive pollensomes contain allergens, such as Ole e 1, Ole e 11, and Ole e 12, suggesting a possible role in allergy. The aim of this study was to assess the contribution of pollensomes to the allergic reaction. We show that pollensomes exhibit allergenic activity in terms of patients' IgE-binding capacity, human basophil activation, and positive skin reaction in sensitized patients. Furthermore, allergen-containing pollensomes have been isolated from three clinically relevant nonphylogenetically related species: birch (Betula verrucosa), pine (Pinus sylvestris), and ryegrass (Lolium perenne). Most interesting, pollensomes were isolated from aerobiological samples collected with an eight-stage cascade impactor collector, indicating that pollensomes secretion is a naturally occurring phenomenon. Our findings indicate that pollensomes may represent widespread vehicles for pollen allergens, with potential implications in the allergic reaction.
  • Publication
    A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
    (Esmon Publicidad, 2010-03) Álvarez García, Elisa; Batanero Cremades, Eva; García Fernández, Rosa; Villalba Díaz, Mayte; Gavilanes, José G.; Martínez del Pozo, Álvaro
    α-Sarcin is a natural variant of Asp f 1 produced by the nonpathogenic fungus Aspergillus giganteus. Both proteins show a sequence identity of 87% and almost identical 3-dimensional structures. α-Sarcin Δ(7-22) is a deletion mutant that displays reduced immunoglobulin (Ig) E reactivity and is much less cytotoxic than wild-type proteins against human transformed cells. Objective: A murine model of sensitization to Asp f 1 was established to test the response elicited by this α-sarcin Δ(7-22) deletion mutant. Methods: BALB/c mice were treated intraperitoneally with different mixtures of recombinant wild-type Asp f 1 and/or a suspension of a commercially available A fumigatus standard extract. Mice were then intranasally challenged with Asp f 1 or α-sarcin Δ(7-22). Sera were collected for subsequent measurement of Ig levels and histological analysis of the nostrils and lungs. Results: Sensitization to Asp f 1 was successful only when the purifi ed protein was fi rst administered together with the A fumigatus suspension. The model was characterized by elevated levels of total IgE in serum and histological lesions in the lungs and nostrils. These symptoms were less severe when the deletion variant was the protein administered, thus confi rming in vivo its lower toxic character. Conclusions: An easily reproducible mouse model of A fumigatus Asp f 1 sensitization was established. This model revealed α-sarcin Δ (7-22) to be a potential candidate for immunotherapy.
  • Publication
    Química Inspirada por la Naturaleza: Lecciones en el Museo Nacional de Ciencias Naturales
    (2017) Batanero Cremades, Eva; Yélamos López, Belén; Fernández Fernández, Mª Inmaculada; López García-Gallo, Pilar; López Rodríguez, Juan Carlos; San Segundo Acosta, Pablo
    Uno de los principales problemas con los que se enfrentan los profesores de Biología es el escaso interés que despierta esta asignatura en los estudiantes universitarios de algunas disciplinas -como química, física, arquitectura o ingeniería- al considerarla una asignatura difícil de aprender y de poca utilidad. Así, motivación, comprensión e interdisciplinaridad son tres retos a los que se enfrenta el profesorado de la asignatura de Biología del Grado en Química. El objetivo general del proyecto ha sido contribuir a la mejora de la enseñanza/aprendizaje de la asignatura de Biología. Para ello el profesorado ha implementado en el aula, y de forma combinada, dos metodologías: La "Biomímesis" y "La enseñanza/aprendizaje basada en Proyectos", que han demostrado ser herramientas muy útiles en el aula, para despertar la motivación y el interés de los estudiantes por el aprendizaje de Biología. Por un lado, al implicarles en su propio proceso de aprendizaje, los estudiantes han tenido un papel protagonista al ser los responsables del desarrollo de proyectos interesantes y retadores, elegidos por ellos mismos. Por otro lado, les ha permitido ver la aplicación o transferencia sostenible de lo aprendido en el aula a la sociedad y el medio ambiente. A estas dos metodologías didácticas se ha unido una tercera: Los Museos de Ciencia Naturales como espacios de enseñanza/aprendizaje de ciencia por investigación. Un total de 2 grupos de teoría han participado en esta experiencia docente. Los trabajos realizados han sido expuestos a otros estudiantes (en el aula) y al público general (en el Museo Nacional de Ciencias Naturales).
  • Publication
    Lactococcus lactis as a vehicle for the heterologous expression of fungal ribotoxin variants with reduced IgE-binding affinity
    (2007-06-25) Álvarez García, Elisa; Alegre Cebollada, Jorge; Batanero Cremades, Eva; Monedero, Vicente; Pérez Martínez, Gaspar; García Fernández, Rosa; Gavilanes, José G.; Martínez del Pozo, Álvaro
    Fungal ribotoxins are a family of extracellular ribonucleases which inhibit protein biosynthesis by inactivating the ribosomes. This inactivation results in the induction of cell death by apoptosis. Ribotoxins show antitumoral properties based on their ability to cross the membrane of some transformed cells. Unfortunately, they also show an unspecific cytotoxicity which has greatly impaired their potential clinical uses. Alfa-sarcin, produced by Aspergillus giganteus, is the best-characterized ribotoxin. Asp f 1, another ribotoxin produced by A. fumigatus, is indeed one of its major allergens. In this work, the Lactococcus lactis MG1363 strain has been engineered to produce and secrete not only wild-type Asp f 1 and -sarcin but also three different mutants with reduced cytotoxicity and/or IgE-binding affinity. The proteins were secreted in native and active form when the extracellular medium employed was buffered at pH values around 8.0. Strains producing the wild-type natural alfa-sarcin were proved to be innocuous when administered intragastrically to mice for a period of 14 days. Overall, the results presented are discussed in terms of its potential application as a vehicle of oral delivery of hypoallergenic variants as well as a starting point to approach the design of strategies to accomplish the safe delivery of these proteins as antitumoral agents.
  • Publication
    ¿Qué es lo que sabemos…sobre Biología III?
    Batanero Cremades, Eva; Castillo Lluva, Sonia; García Álvarez, Begoña; Pérez Gómez, Eduardo; Yélamos López, María Belén
    El inglés y las tecnologías móviles son fundamentales hoy día. Los estudiantes utilizarán dichas tecnologías para motivar y reforzar el aprendizaje de Biología mediante dos actividades: crear cuestionarios Kahoot! en inglés y emplearlos en concursos.
  • Publication
    Ash pollen immunoproteomics: Identification, immunologic characterization, and sequencing of 6 new allergens
    (Elsevier, 2014) Mas García, Salvador; Torres Pardo, María; Garrido-Arandia, María; Salamanca, Guillermo; Castro, Lourdes; Barral, Patricia; Purohit, Ashok; Pauli, Gabrielle; Rodríguez García, Rosalía; Batanero Cremades, Eva; Barderas Manchado, Rodrigo; Villalba Díaz, Mayte
    Immunoproteomics, IgE-inhibition assays and cDNA-cloning reveals that ash and olive allergenic protein profiles are mostly equivalent, thus explaining their high cross reactivity. Our data suggest simplifying diagnosis of patients by using indistinctly ash or olive pollen.
  • Publication
    ¿Qué es lo que sabemos… sobre Biología? IV
    Aguado Sánchez, Tania; Batanero Cremades, Eva; Castillo Lluva, Sonia; García Álvarez, Begoña; Palazuelos Diego, Javier; Pérez Gómez, Eduardo; Sánchez García, Cristina; Yélamos López, María Belén
    El inglés y las tecnologías móviles son fundamentales hoy día. Los estudiantes utilizarán dichas tecnologías para motivar y reforzar el aprendizaje de Biología mediante dos actividades: crear cuestionarios Kahoot! en inglés y emplearlos en concursos.
  • Publication
    “¿Qué es lo que sabemos… sobre Biología”
    (2020-05-18) Batanero Cremades, Eva; Rodríguez Crespo, José Ignacio; San Segundo Acosta, Pablo; Bueno Díaz, Cristina; Parrón Ballesteros, Jorge
    Dada la importancia del inglés y de las tecnologías móviles hoy día, los estudiantes utilizarán dichas tecnologías para reforzar el aprendizaje de Biología, y motivarlos a estudiar, mediante dos actividades: crear test Kahoot en inglés y concursar.