Person: Gómez Sala, Beatriz
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First Name
Beatriz
Last Name
Gómez Sala
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Department
Area
Nutrición y Bromatología
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
Item Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm(Microorganisms, 2022) Feito Hermida, Javier; Contente, Diogo; Ponce Alonso, Manuel; Díaz Formoso, Lara; Araújo, Carlos; Peña Vidal, Nuria; Borrero Del Pino, Juan; Gómez Sala, Beatriz; Campo, Rosa del; Muñoz Atienza, Estefanía; Hernández Cruza, Pablo Elpidio; Cintas Izarra, Luis MiguelProbiotics are a viable alternative to traditional chemotherapy agents to control infectious diseases in aquaculture. In this regard, Lactococcus lactis subsp. cremoris WA2-67 has previously demonstrated several probiotic features, such as a strong antimicrobial activity against ichthyopathogens, survival in freshwater, resistance to fish bile and low pH, and hydrophobicity. The aim of this manuscript is an in silico analysis of the whole-genome sequence (WGS) of this strain to gain deeper insights into its probiotic properties and their genetic basis. Genomic DNA was purified, and libraries prepared for Illumina sequencing. After trimming and assembly, resulting contigs were subjected to bioinformatic analyses. The draft genome of L. cremoris WA2-67 consists of 30 contigs (2,573,139 bp), and a total number of 2493 coding DNA sequences (CDSs). Via in silico analysis, the bacteriocinogenic genetic clusters encoding the lantibiotic nisin Z (NisZ) and two new bacteriocins were identified, in addition to several probiotic traits, such as the production of vitamins, amino acids, adhesion/aggregation, and stress resistance factors, as well as the absence of transferable antibiotic resistance determinants and genes encoding detrimental enzymatic activities and virulence factors. These results unveil diverse beneficial properties that support the use of L. cremoris WA2-67 as a probiotic for aquaculture.Item Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae(Foods, 2023) Feito Hermida, Javier; Araújo, Carlos; Arbulu, Sara; Contente, Diogo; Gómez Sala, Beatriz; Díaz-Formoso, Lara; Muñoz Atienza, Estefanía; Borrero Del Pino, Juan; Cintas Izarra, Luis Miguel; Hernández Cruza, Pablo ElpidioLactococcus garvieae is a main ichthyopathogen in rainbow trout (Oncorhynchus mykiss, Walbaum) farming, although bacteriocinogenic L. garvieae with antimicrobial activity against virulent strains of this species have also been identified. Some of the bacteriocins characterized, such as garvicin A (GarA) and garvicin Q (GarQ), may show potential for the control of the virulent L. garvieae in food, feed and other biotechnological applications. In this study, we report on the design of Lactococcus lactis strains that produce the bacteriocins GarA and/or GarQ, either alone or together with nisin A (NisA) or nisin Z (NisZ). Synthetic genes encoding the signal peptide of the lactococcal protein Usp45 (SPusp45), fused to mature GarA (lgnA) and/or mature GarQ (garQ) and their associated immunity genes (lgnI and garI, respectively), were cloned into the protein expression vectors pMG36c, which contains the P32 constitutive promoter, and pNZ8048c, which contains the inducible PnisA promoter. The transformation of recombinant vectors into lactococcal cells allowed for the production of GarA and/or GarQ by L. lactis subsp. cremoris NZ9000 and their co-production with NisA by Lactococcus lactis subsp. lactis DPC5598 and L. lactis subsp. lactis BB24. The strains L. lactis subsp. cremoris WA2-67 (pJFQI), a producer of GarQ and NisZ, and L. lactis subsp. cremoris WA2-67 (pJFQIAI), a producer of GarA, GarQ and NisZ, demonstrated the highest antimicrobial activity (5.1- to 10.7-fold and 17.3- to 68.2-fold, respectively) against virulent L. garvieae strains.- Project number: 25
Item Hacia un massive online open course (MOOC) para el aprendizaje de la higiene y seguridad alimentarias(2021) Borrero Del Pino, Juan; Chaparro Domínguez, María De Los Ángeles; Cintas Izarra, Luis Miguel; Contente de Matos, Diogo da Silva Serra; Feito Hermida, Javier; Hernández Cruza, Pablo Elpidio; Marín Martínez, María; Muñoz Atienza, Estefanía; Martín De Santos, María Del Rosario; Peña Vidal, Nuria; Gómez Sala, Beatriz; Álvarez López, Alberto; Celorrio de Ochoa, David; Alache Manchado, Beatriz; Ávila Alonso, Óscar; Beltrán Crespo, Antonio; Blanco Palmero, Felipe A.; Cabrales Miró-Granada, Ana; Carabante Oliver, Natalia; Carmona Agraz, Ana; Carpintero Cagigas, Ana; Casarrubios Guío, Pilar; Escobar Sáez, Daniel; Palenzuela González, Cristina; Romero Domingo, Sandra Item Antimicrobial Activity, Genetic Relatedness, and Safety Assessment of Potential Probiotic Lactic Acid Bacteria Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis) Used as Live Feed in Fish Larviculture(Animals, 2024) Contente, Diogo; Díaz Formoso, Lara; Feito Hermida, Javier; Gómez Sala, Beatriz; Costas, Damián; Hernández Cruza, Pablo Elpidio; Muñoz Atienza, Estefanía; Borrero Del Pino, Juan; Poeta, Patrícia; Cintas Izarra, Luis MiguelAquaculture is a rapidly expanding agri-food industry that faces substantial economic losses due to infectious disease outbreaks, such as bacterial infections. These outbreaks cause disruptions and high mortalities at various stages of the rearing process, especially in the larval stages. Probiotic bacteria are emerging as promising and sustainable alternative or complementary strategies to vaccination and the use of antibiotics in aquaculture. In this study, potential probiotic candidates for larviculture were isolated from a rotifer-rearing tank used as the first live feed for turbot larvae. Two Lacticaseibacillus paracasei and two Lactiplantibacillus plantarum isolates were selected for further characterization due to their wide and strong antimicrobial activity against several ichthyopathogens, both Gram-positive and Gram-negative. An extensive in vitro safety assessment of these four isolates revealed the absence of harmful traits, such as acquired antimicrobial resistance and other virulence factors (i.e., hemolytic and gelatinase activities, bile salt deconjugation, and mucin degradation, as well as PCR detection of biogenic amine production). Moreover, Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) analyses unveiled their genetic relatedness, revealing two divergent clusters within each species. To our knowledge, this work reports for the first time the isolation and characterization of Lactic Acid Bacteria (LAB) with potential use as probiotics in aquaculture from rotifer-rearing tanks, which have the potential to optimize turbot larviculture and to introduce novel microbial management approaches for a sustainable aquaculture.- Project number: 262
Item Diseño y desarrollo de una herramienta audiovisual para la docencia virtual de la inspección veterinaria oficial de pescados y productos de la pesca en un mercado central(2022) Muñoz Atienza, Estefanía; Yagüe Sánchez, Ángel; Álvarez López, Alberto; Borrero Del Pino, Juan; García García, Aina; García Lacarra, Teresa; Hernández Cruza, Pablo Elpidio; Marín Martínez, María; Martín De Santos, María Del Rosario; Dias Araújo, Carlos; Díaz Formoso, Lara; Cintas Izarra, Luis Miguel; da Silva Serra Contente de Matos, Diogo; Feito Hermida, Javier; García Calvo, Eduardo Rafael; Gómez Sala, Beatriz; Lafuente Orte, Irene; Peña Vidal, Nuria; Rodríguez Gómez, Santiago; Sevillano González, Ester; Beltrán Crespo, Antonio; Caba Manzaneque, Elia; Cabrales Miró-Granada, Ana; Cano Muñoz, Marisa; Cañizares Cooz, Daniela; Celorrio de Ochoa, David; Dorado Nuñez, Gemma; Fernández Silva, Ana; Gutiérrez de Cabiedes de Fuente, Alejandro; Márquez Bayón, Teresa; Martín Martí, Laura; Olmeda García, Patricia; Roncero Fernández, Alejandro Francisco; Sánchez Prada, Raquel; Taberneiro Auiget, DanielEl objetivo global de este Proyecto de Innovación Docente es la creación de vídeos explicativos como una herramienta de aprendizaje incorporada en el Campus Virtual para mejorar el estudio sobre las actividades de higiene, inspección y control alimentario que se realizan en el Mercado Central de Pescados de Mercamadrid. La creación y el empleo de estos vídeos están dirigidos, en un principio, a los estudiantes universitarios de Grado en Veterinaria que cursan la asignatura de Higiene, Inspección y Seguridad Alimentaria. En este Proyecto se han creado vídeos explicativos que tratan sobre: (i) los controles oficiales realizados por los Técnicos Superiores Veterinarios de Mercamadrid; (ii) los riesgos sanitarios asociados al consumo de pescados, crustáceos y moluscos; (iii) la frescura del pescado; (iv) el etiquetado del pescado; (v) la identificación de especies de pescado y marisco; (vi) la prevención de fraudes en la comercialización de pescados y mariscos; y (vii) la autentificación de pescados fileteados mediante técnicas de análisis.