Person: Moreno Bondi, María Cruz
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First Name
María Cruz
Last Name
Moreno Bondi
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
Identifiers
9 results
Search Results
Now showing 1 - 9 of 9
Publication Aluminum Nanoholes for Optical Biosensing(MDPI, 2015-07-09) Barrios, Carlos; Canalejas-Tejero, Víctor; Herranz, Sonia; Urraca Ruiz, Javier; Moreno Bondi, María Cruz; Avella-Oliver, Miquel; Maquieira, Ángel; Puchades, RosaSub-wavelength diameter holes in thin metal layers can exhibit remarkable optical features that make them highly suitable for (bio)sensing applications. Either as efficient light scattering centers for surface plasmon excitation or metal-clad optical waveguides, they are able to form strongly localized optical fields that can effectively interact with biomolecules and/or nanoparticles on the nanoscale. As the metal of choice, aluminum exhibits good optical and electrical properties, is easy to manufacture and process and, unlike gold and silver, its low cost makes it very promising for commercial applications. However, aluminum has been scarcely used for biosensing purposes due to corrosion and pitting issues. In this short review, we show our recent achievements on aluminum nanohole platforms for (bio)sensing. These include a method to circumvent aluminum degradation—which has been successfully applied to the demonstration of aluminum nanohole array (NHA) immunosensors based on both, glass and polycarbonate compact discs supports—the use of aluminum nanoholes operating as optical waveguides for synthesizing submicron-sized molecularly imprinted polymers by local photopolymerization, and a technique for fabricating transferable aluminum NHAs onto flexible pressure-sensitive adhesive tapes, which could facilitate the development of a wearable technology based on aluminum NHAs.Publication Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples(Wiley-VCH, 2022-02-17) Luque Uría, Álvaro; Peltomaa, Riikka; Navarro Duro, Marina; Fikacek, Sabrina; Head, Trajen; Deo, Sapna K.; Daunert, Sylvia; Benito Peña, María Elena; Moreno Bondi, María CruzThe development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.Publication Biosensing Based on Nanoparticles for Food Allergens Detection(MDPI, 2018-04-04) Gómez Arribas, Lidia Nazaret; Benito Peña, María Elena; Hurtado Sánchez, María del Carmen; Moreno Bondi, María CruzFood allergy is one of the major health threats for sensitized individuals all over the world and, over the years, the food industry has made significant efforts and investments to offer safe foods for allergic consumers. The analysis of the concentration of food allergen residues in processing equipment, in raw materials or in the final product, provides analytical information that can be used for risk assessment as well as to ensure that food-allergic consumers get accurate and useful information to make their food choices and purchasing decisions. The development of biosensors based on nanomaterials for applications in food analysis is a challenging area of growing interest in the last years. Research in this field requires the combined efforts of experts in very different areas including food chemistry, biotechnology or materials science. However, the outcome of such collaboration can be of significant impact on the food industry as well as for consumer’s safety. These nanobiosensing devices allow the rapid, selective, sensitive, cost-effective and, in some cases, in-field, online and real-time detection of a wide range of compounds, even in complex matrices. Moreover, they can also enable the design of novel allergen detection strategies. Herein we review the main advances in the use of nanoparticles for the development of biosensors and bioassays for allergen detection, in food samples, over the past few years. Research in this area is still in its infancy in comparison, for instance, to the application of nanobiosensors for clinical analysis. However, it will be of interest for the development of new technologies that reduce the gap between laboratory research and industrial applications.Publication Diseño y preparación de un laboratorio virtual de Química Analítica: Técnicas instrumentales de análisis(2022-01-30) Benito Peña, Elena; Burgueño Arjona, Maria José; Campuzano Ruiz, Susana; Madrid Albarrán, María Yolanda; Marazuela Lamata, María Dolores; Mateos Briz, María Raquel; Moreno Bondi, María Cruz; Navarro Villoslada, Fernando; Pedrero Muñoz, María; Reviejo García, Ángel Julio; Tortajada Pérez, José; Urraca Ruiz, Javier; Villalonga Santana, Reynaldo; Blanco Asenjo, Miriam; del Valle Ávila, Marcos; Molla Escudero, David; Navarro Duro, Marina; del Rosario García-Marcos, Beatriz; Merino Sierra, Miguel Ángel; García López, PatriciaLa reciente pandemia del COVID-19 ha supuesto un cambio excepcional y drástico de la concepción tradicional del aprendizaje, tanto para los estudiantes como para los docentes. Ante esta situación se requiere no sólo acciones que faciliten la adaptación de los estudiantes y profesores a las plataformas educativas en línea, sino también a que éstas se conviertan en auténticas herramientas para potenciar y mejorar de forma significativa el aprendizaje del alumno. El presente proyecto de Innovación Educativa y Mejora de la Calidad Docente pretende mejorar la calidad del aprendizaje de varias asignaturas de los Grados de Química e Ingeniería Química que llevan asociado un Laboratorio de Técnicas Instrumentales. Tradicionalmente esa docencia práctica ha sido presencial, pero la situación excepcional surgida con la pandemia en el curso 2019/2020, ha demostrado que disponer de unas prácticas virtualizadas es de gran ayuda para facilitar el aprendizaje de los alumnos y facilitar una transferencia del conocimiento constructivo y colaborativo. Desde hace años, el personal docente e investigador (PDI), personal de administración y servicios (PAS) y los estudiantes de la UCM, disponemos de la plataforma de enseñanza online Moodle (Campus Virtual, CV). Sin embargo, la pandemia que vivimos ha evidenciado la brecha digital en lo referente a cómo usar Moodle y, por ende, la utilización de las Tecnologías de Información y Comunicación (TICs) como facilitadoras didácticas. A pesar de nuestras limitaciones, tanto profesores como estudiantes, hemos desarrollado una enorme capacidad resiliente, lo que permitió, durante el pasado mes de mayo, la puesta en marcha de los primeros laboratorios en línea en el Departamento de Química Analítica de la UCM. Fruto de esta experiencia, así como de la situación actual de incertidumbre para el curso próximo, algunos profesores, estudiantes y PAS del departamento hemos decidido adelantarnos a un escenario futuro en el que se contemple nuevamente la impartición de Docencia Experimental en línea y solicitar el presente proyecto de Innovación Educativa y Mejora de la Calidad Docente.Publication "Chem-game", el juego como estrategia para la dinamización del aprendizaje y la evaluación de conocimientos en Química General(2020-05-18) Moreno Bondi, María Cruz; Gutiérrez Alonso, Angel; Lainez Ferrando, Alfredo; Benito Peña, María Elena; Glahn Martínez, Ana Bettina; García Pinar, Gioele; Castellano Perdomo, Raúl; Navarro Duro, Marina; Peng, Wenrong; Morales Benítez, Noelia; Rubio Ramón, Pablo Ignacio; Olmos Alonso, InésEste proyecto pretende aplicar la "gamificación" en la enseñanza de la asignatura de Química General de primer curso del grado en Química para fomentar la formación, creatividad, compromiso y la capacidad de trabajo en equipo de los estudiantes.Publication Material docente interactivo en inglés para la enseñanza práctica y el autoaprendizaje de (bio)sensores químicos ópticos en Grado y Máster(2018-01-15) Descalzo López, Ana Belén; Orellana Moraleda, Guillermo; Moreno Bondi, María Cruz; Benito Peña, María Elena; Urraca Ruiz, JavierMaterial docente (guiones de prácticas, vídeos, cuestionarios multi-respuesta) en inglés para asignaturas de Grado y Máster relacionadas con el tema de sensores (bio)químicos ópticos y la preparación de nanomateriales aplicados en sensores ópticosPublication Recombinant Peptide Mimetic NanoLuc Tracer for Sensitive Immunodetection of Mycophenolic Acid(ACS Publications, 2021-07-14) Luque Uría, Álvaro; Peltomaa, Riikka; Nevanen, Tarja K.; Arola, Henri O.; Iljin, Kristiina; Benito Peña, Elena; Moreno Bondi, María CruzMycophenolic acid (MPA) is an immunosuppressant drug commonly used to prevent organ rejection in transplanted patients. MPA monitoring is of great interest due to its small therapeutic window. In this work, a phage-displayed peptide library was used to select cyclic peptides that bind to the MPA-specific recombinant antibody fragment (Fab) and mimic the behavior of MPA. After biopanning, several phage-displayed peptides were isolated and tested to confirm their epitope-mimicking nature in phagebased competitive immunoassays. After identifying the best MPA mimetic (ACEGLYAHWC with a disulfide constrained loop), several immunoassay approaches were tested, and a recombinant fusion protein containing the peptide sequence with a bioluminescent enzyme, NanoLuc, was developed. The recombinant fusion enabled its direct use as the tracer in competitive immunoassays without the need for secondary antibodies or further labeling. A bioluminescent sensor, using streptavidin-coupled magnetic beads for the immobilization of the biotinylated Fab antibody, enabled the detection of MPA with a detection limit of 0.26 ng mL−1 and an IC50 of 2.9 ± 0.5 ng mL−1 . The biosensor showed good selectivity toward MPA and was applied to the analysis of the immunosuppressive drug in clinical samples, of both healthy and MPA-treated patients, followed by validation by liquid chromatography coupled to diode array detectionPublication Elaboración de una biblioteca audiovisual para la enseñanza de la Química Analítica(Editorial Complutense, 2007) Moreno Bondi, María Cruz; Palacios Corvillo , María Antonia; Pérez Conde , Concepción; Gutiérrez Carreras , Ana María; Manuel de Villena Rueda , Francisco Javier; Pedrero Muñoz , María; González Cortés, Araceli; Agüí Chicharro , María Lourdes; Rodríguez Marchán, A. I.; Rosende Novo, A.; Fernández-Valmayor Crespo, Alfredo; Fernández-Pampillón Cesteros, Ana María; Merino Granizo, JorgeEl Producto Final elaborado en este proyecto forma parte de una biblioteca audiovisual que recogerá los aspectos básicos de las enseñanzas prácticas impartidas en Química Analítica en la Licenciatura en Ciencias Químicas y en Ingeniería Química. Se trata de reforzar la labor docente del Departamento y facilitar así la enseñanza del alumno en esta disciplina.Publication Optical Biosensors for Label-Free Detection of Small Molecules(MDPI, 2018-11-24) Peltomaa, Riikka Johanna; Glahn Martínez, Ana Bettina; Benito Peña, María Elena; Moreno Bondi, María CruzLabel-free optical biosensors are an intriguing option for the analyses of many analytes, as they offer several advantages such as high sensitivity, direct and real-time measurement in addition to multiplexing capabilities. However, development of label-free optical biosensors for small molecules can be challenging as most of them are not naturally chromogenic or fluorescent, and in some cases, the sensor response is related to the size of the analyte. To overcome some of the limitations associated with the analysis of biologically, pharmacologically, or environmentally relevant compounds of low molecular weight, recent advances in the field have improved the detection of these analytes using outstanding methodology, instrumentation, recognition elements, or immobilization strategies. In this review, we aim to introduce some of the latest developments in the field of label-free optical biosensors with the focus on applications with novel innovations to overcome the challenges related to small molecule detection. Optical label-free methods with different transduction schemes, including evanescent wave and optical fiber sensors, surface plasmon resonance, surface-enhanced Raman spectroscopy, and interferometry, using various biorecognition elements, such as antibodies, aptamers, enzymes, and bioinspired molecularly imprinted polymers, are reviewed.