Person: Mateo Barrientos, Marta
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First Name
Marta
Last Name
Mateo Barrientos
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Microbiología y Parasitología
Area
Parasitología
Identifiers
8 results
Search Results
Now showing 1 - 8 of 8
Item Detection and Molecular Diversity of Cryptosporidium spp. and Giardia duodenalis in the Endangered Iberian Lynx (Lynx pardinus), Spain(Animals, 2024) Matas Méndez, Pablo; Ávalos, Gabriel; Caballero Gómez, Javier; Dashti, Alejandro; Castro-Scholten, Sabrina; Jiménez Martín, Débora; González Barrio, David; Muñoz de Mier, Gemma J.; Bailo Barroso, Begoña; Cano-Terriza, David; Mateo Barrientos, Marta; Nájera, Fernando; Xiao, Lihua; Köster, Pamela C.; García Bocanegra, Ignacio; Carmena, DavidCryptosporidium spp. and Giardia duodenalis are the main non-viral causes of diarrhoea in humans and domestic animals globally. Comparatively, much less information is currently available in free-ranging carnivore species in general and in the endangered Iberian lynx (Lynx pardinus) in particular. Cryptosporidium spp. and G. duodenalis were investigated with molecular (PCR and Sanger sequencing) methods in individual faecal DNA samples of free-ranging and captive Iberian lynxes from the main population nuclei in Spain. Overall, Cryptosporidium spp. and G. duodenalis were detected in 2.4% (6/251) and 27.9% (70/251) of the animals examined, respectively. Positive animals to at least one of them were detected in each of the analysed population nuclei. The analysis of partial ssu rRNA gene sequences revealed the presence of rodent-adapted C. alticolis (n = 1) and C. occultus (n = 1), leporid-adapted C. cuniculus (n = 2), and zoonotic C. parvum (n = 2) within Cryptosporidium, and zoonotic assemblages A (n = 5) and B (n = 3) within G. duodenalis. Subgenotyping analyses allowed for the identification of genotype VaA19 in C. cuniculus (gp60 locus) and sub-assemblages AI and BIII/BIV in G. duodenalis (gdh, bg, and tpi loci). This study represents the first molecular description of Cryptosporidium spp. and G. duodenalis in the Iberian lynx in Spain. The presence of rodent/leporid-adapted Cryptosporidium species in the surveyed animals suggests spurious infections associated to the Iberian lynx’s diet. The Iberian lynx seems a suitable host for zoonotic genetic variants of Cryptosporidium (C. parvum) and G. duodenalis (assemblages A and B), although the potential risk of human transmission is regarded as limited due to light parasite burdens and suspected low excretion of infective (oo)cysts to the environment by infected animals. More research should be conducted to ascertain the true impact of these protozoan parasites in the health status of the endangered Iberian lynx.Item Prevalence and public health relevance of enteric parasites in domestic dogs and cats in the region of Madrid (Spain) with an emphasis on Giardia duodenalis and Cryptosporidium sp.(Veterinary Medicine and Science, 2023) Mateo Barrientos, Marta; Montoya Matute, Ana; Bailo Barroso, Begoña; Köster, Pamela C.; Dashti, Alejandro; Hernández Castro, Carolina; Saugar, José M.; Matas, Pablo; Xiao, Lihua; Carmena, DavidBackground: Pet dogs and cats exert an unquestionable beneficial effect in the well-being of their owners, but can also act as a source of zoonotic infections if improperly cared. Objectives: We investigated the occurrence, risk factors, genetic variability and zoonotic potential of intestinal parasites in dogs and cats attended in a clinical veterinary setting in Spain. Methods: Canine (n = 252) and feline (n = 35) faecal samples were collected during 2017–2019 and analysed by coproparasitological methods. A rapid lateral immunochromatographic test (ICT) was used for detecting Giardia duodenalis and Cryptosporidium sp. Samples positive at microscopy examination and/or ICT were reassessed by molecular methods. Results: Overall, 48.8% (123/252) of dogs and 48.6% (17/35) of cats were infected by enteric parasites. In dogs, G. duodenalis was the most prevalent species (40.9%), followed by Cystoisospora sp. (7.1%), and Toxocara canis (5.2%). In cats, Joyeuxiella sp. and Toxocara cati were the dominant species (20.0% each), followed by G. duodenalis (14.3%), D. caninum (5.7%) and Cystoisospora felis and Toxascaris leonina (2.9% each). Pups and kittens were more likely to harbour intestinal parasites and develop clinical signs. Sequence analyses of dog isolates revealed the presence of assemblages A (n = 1), C (n = 4), D (n = 4) and C+D (n = 1) within G. duodenalis; C. parvum (n = 1) and C. canis (n = 4) within Cryptosporidium and PtEb IX (n = 1) in Enterocytozoon bieneusi. A novel C. canis subtype family, named XXi, is reported. Conclusions: Our results highlight that (i) well-cared dogs carry zoonotic enteric protozoan parasites of public health relevance, (ii) proper hygiene practices and routine veterinary treatment are essential to prevent zoonotic infections, (iii) vulnerable populations should avoid contact with pups/kittens with diarrhoea and (iv) infected dogs might be major contributors to the environmental contamination with soil-transmitted helminths (STHs) eggs.Item Occurrence of Leishmania infantum in Wild Mammals Admitted to Recovery Centers in Spain(Pathogens, 2023) Azami Conesa, Iris; Pérez Moreno, Paula; Matas Méndez, Pablo; Sansano-Maestre, Jose; González González, Fernando; Mateo Barrientos, Marta; Gómez Muñoz, María TeresaZoonotic leishmaniasis caused by Leishmania infantum is distributed worldwide and affects humans and domestic and wild mammals. In Europe, specifically in the Mediterranean basin, leishmaniasis is endemic due to the concurrence of the phlebotomine vectors and reservoir mammals, including carnivorous wildlife species and other less studied wild species. In this article, spleen, skin, and eye or oral swabs taken from 134 wild mammals admitted to five wildlife recovery centers in Spain were used. PCR employing fragments of the Repeat region, ITS1, and SSUrRNA were used for detection, and positive samples were processed for sequencing. L. infantum was detected in three out of the nine species analyzed, including European hedgehog, European badger, and red squirrel, with percentages ranging from 11.53 to 35.71%, depending on the species. Most of the species showed higher percentages of positivity in spleen samples than in skin samples. A small number of animals from the remaining six species tested negative, including Algerian hedgehog, stone marten, least weasel, garden dormouse, western polecat, and Egyptian mongoose. Hedgehogs and badgers are good candidates for consideration as epidemiological sentinels and pose a higher risk as potential reservoirs of leishmaniasis based on their percentage of infection and wide distribution.Item Molecular Characterization of Enterocytozoon bieneusi in Wild Carnivores in Spain(Journal of Eukaryotic Microbiology, 2017) Santín, Mónica; Calero Bernal, Rafael; Carmena, David; Mateo Barrientos, Marta; Balseiro, Ana; Barral, Marta; Lima Barbero, José Francisco; Habela, Miguel ÁngelMicrosporidia comprises a diverse group of obligate intracellular parasites that infect a broad range of invertebrates and vertebrates. Among Microsporidia, Enterocytozoon bieneusi is the most frequently detected species in humans and animals worldwide bringing into question the possible role of animal reservoirs in the epidemiology of this pathogen. Although E. bieneusi is an emerging zoonotic pathogen able to infect many domestic and wild mammals that could act as reservoir of infection for humans and other animals, only few studies have documented its occurrence in wild carnivores. To determine the occurrence of E. bieneusi in wild carnivores, we examined 190 wild carnivores collected from different locations in Spain. Twenty-five fecal samples (13.2%) from three host species (European badger, beech marten, and red fox) were E. bieneusi-positive by PCR. Nucleotide sequence analysis of the ITS region revealed a high degree of genetic diversity with a total of eight distinct genotypes including four known (PtEbIX, S5, S9, and WildBoar3) and four novel (EbCar1-EbCar4) genotypes identified. Phylogenetic analysis showed that the four novel genotypes (EbCar1-EbCar4), S5, S9, and WildBoar3 clustered within the previously designated zoonotic Group 1. Our results demonstrate that human-pathogenic genotypes are present in wild carnivores, corroborating their potential role as a source of human infection and environmental contamination.Item Prevalence of Toxoplasma gondii in Endangered Wild Felines (Felis silvestris and Lynx pardinus) in Spain(Animals, 2023) Matas Méndez, Pablo; Fuentes Corripio, Isabel; Montoya Matute, Ana; Bailo Barroso, Begoña; Grande Gómez, Rebeca; Apruzzese Rubio, Ariadna; Ponce Gordo, Francisco; Mateo Barrientos, MartaThe wildcat (Felis silvestris) and the Iberian lynx (Lynx pardinus) are important species in Spain, considered as near-threatened and endangered, respectively. Both can be infected by Toxoplasma gondii, a parasite that can cause morbidity and mortality in transplacentally-infected or immunocompromised mammals. The data on the prevalence of this parasite in wild populations of these species in Spain are outdated. The objective of this study was to update information and evaluate the role of these felines in parasite epidemiology and the potential impact of the parasite on their conservation. Blood and fecal samples were collected from captured animals, as well as the tongue, diaphragm, and spleen, from animals killed in road accidents in central Spain. An indirect fluorescent antibody test (IFAT) was used to detect parasite antibodies in serum, microscopy and molecular analysis were used to detect oocysts in feces, and molecular analysis was used to determine the existence of tissue cysts. Seroprevalence was 85% in wildcats and 45% in lynx, and parasite DNA was detected in the feces of one wildcat and in tissue samples from 10 wildcats and 11 Iberian lynxes. These results highlight the epidemiological importance and high risk of T. gondii infection in animals and humans in the studied areas. Considering feline susceptibility to infection, monitoring programs are needed to assess the health status of wild felinesItem Microbial Matryoshka: Addressing the Relationship between Pathogenic Flagellated Protozoans and Their RNA Viral Endosymbionts (Family Totiviridae).(Veterinary Sciences, 2024) Ibáñez Escribano, Alexandra; Gómez Muñoz, María Teresa; Mateo Barrientos, Marta; Fonseca Berzal, Cristina Rosa; Gómez-Lucía Duato, María Esperanza; García-Pérez, Raquel; Alunda Rodríguez, José María; Carrión Herrero, Francisco JavierGiardiosis, trichomonosis, leishmaniosis, and trypanosomosis are parasitic diseases caused by flagellated protozoa that have a major global health impact, and their control is a priority action line in the agenda of the current One Health Program. The pathogens causing these diseases can establish an endosymbiotic relationship with RNA viruses of the Totiviridae family that can alter the course of the final infection in a mammal. To easily understand the sequence of interactions that occur between the agents involved, from a structural point of view, we can imagine a “matryoshka”-type infection model, wherein the virus represents the smallest matryoshka infecting the flagellated protozoan, which represents the medium matryoshka infecting the mammal, the largest matryoshka. In this manuscript, we will review the available information on the complications generated, such as the aggravation of pathogenesis or treatment failures, because of the established association between these flagellated pathogens and their respective endosymbiont viruses. Accurate diagnosis is required to detect these situations of endosymbiont co-infection and to be able to apply tailor-made treatments that target both the flagellated parasite and the virus that hides inside it. Taken together, these approaches will allow us to achieve and optimize appropriate sanitary control strategies.Item West Nile Virus Seroprevalence in Wild Birds and Equines in Madrid Province, Spain(Veterinary Sciences, 2024) Williams, Richard Alexander John; Criollo Valencia, Hillary A.; López Márquez, Irene; González González, Fernando; Llorente, Francisco; Jiménez Clavero, Miguel Ángel; Busquets, Núria; Mateo Barrientos, Marta; Ortiz Díez, Gustavo; Ayllón Santiago, TaniaSimple Summary: West Nile virus (WNV) is a flavivirus that circulates among birds and mosquitoes and can cause outbreaks in people and horses, sometimes leading to serious brain-related illness and death. This study aimed to investigate WNV circulation in birds and horses in Madrid, Spain. Through serological testing (cELISA), a proportion of birds were positive for WNV antibodies, indicating potential exposure. Four birds and one horse were confirmed positive for WNV antibodies with a second test, while four other birds showed antibodies to an undetermined flavivirus. Because birds positive for WNV antibodies were adults, they might have been exposed elsewhere. The horse had previously lived in a WNV endemic zone. The birds positive for flavivirus included two young birds that had not traveled outside Madrid. The presence of antibodies in two juvenile birds that could hardly fly suggests local circulation of flavivirus in birds in Madrid. The study addresses the potential circulation of WNV or related flaviviruses in birds in Madrid, emphasizing the need for increased surveillance to understand transmission dynamics and the principal species involved. Given the growing incidence and spread of WNV in Spain, continued research is vital for risk assessment and implementing effective control measures.- Project number: PIMCD276/23-24
Item Telegram como herramienta docente en Parasitología e Inmunología(2024) Fonseca Berzal, Cristina Rosa; González Fernández, Juan; Ibáñez Escribano, Alexandra; Gómez Barrio, Alicia; Escario García-Trevijano, José Antonio; Cuéllar Del Hoyo, María Del Carmen; García Rodríguez, Juan José; Mateo Barrientos, Marta; Jiménez Alonso, María Isabel; Martínez Grueiro, Mercedes; Martínez Grueiro, Mercedes; Nogal Ruiz, Juan José; Ponce Gordo, Francisco; Rodero Martínez, Marta; Hernández Fuentes, Maria Donina; Hernández García, Francisco Javier; Pumar Martín, Manuela; Espinosa de los Monteros Buitrago, Teresa; Checa Herráiz, Rocío