Person: Pérez Sancho, Marta
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First Name
Marta
Last Name
Pérez Sancho
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
38 results
Search Results
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Item Non-invasive surveillance of shared pathogens in the Eurasian brown bear (Ursus arctos) human interface(One Health, 2024) Herrero García, Gloria; Barroso, Patricia; Dashti, Alejandro; González Barrio, David; Naves, Javier; Fernández Gil, Alberto; Ugarte Ruiz, María; Pérez Sancho, Marta; Royo, Luis José; Carmena, David; De Miguel, Arturo; García Rodríguez, Alberto; Gortázar, Christian; Domínguez Rodríguez, Lucas José; Balseiro, AnaMulti-host communities are perfect scenarios for the emergence and spread of pathogens, threatening the recovery of endangered, isolated, or inbred populations, such as the brown bear (Ursus arctos) in northwestern Spain. The population recovery in recent years has forced bears to occupy highly anthropized areas, increasing their interaction with human and domestic animals, with potential consequences for global health. During 2022–2023 a survey of parasites, bacteria and viruses shared between wildlife, domestic animals and humans was performed in this population using non-invasive surveillance, i.e., bear fecal samples (n = 73) and sponge-based sampling of trees (n = 42; 14 rubbed trees and 28 control trees). Pathogen detection rates were defined as the percentage of qPCR or culture-positive samples. Generalized linear models were fitted to assess their relationship with environmental variables including dispersion of the human population, and percentage of agricultural and periurban habitats in a 6 km-buffer around each sample. Canine Adenovirus type 1 (45.2%), Giardia spp. (15.1%), Salmonella spp. (12.3%), and extended-spectrum-beta-lactamases (ESBL) Escherichia coli (1.4%) were identified in fecal samples. In contrast, only five sponges from three rubbed and two control trees resulted positive to E. coli (14.3%). The results suggest that several pathogens are common in the Cantabrian brown bear population and that anthropization of the territory modulates their prevalence and richness. The effective design of management programs for bear conservation will require a one-health approach, in which genetic analysis of non-invasive samples can be key tools for the sanitary surveillance at the wildlife-livestock-human interface.Item Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk(Research in Veterinary Science, 2020) Roy, A.; Infantes-Lorenzo, J.A.; Dominguez, M.; Moreno, I.; Pérez Sancho, Marta; García Benzaquén, Nerea; García-Seco Romero, María Teresa; Álvarez Sánchez, Julio; Romero Martínez, Beatriz; Gortázar, C.; Juan Ferré, Lucía De; Domínguez Rodríguez, Lucas José; Bezos Garrido, JavierCaprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity under specific circumstances. In this study, performance of a new experimental test based on the P22 protein complex (P22 ELISA) was evaluated in two TB-infected herds using milk and serum samples and compared with cell-based diagnostic tests. Samples from a low (n = 62, herd 1) and a high (n = 52, herd 2) TB prevalence herd were selected. Moreover, bulk tank milk samples from both herds were analysed using the P22 ELISA. At the end of the study, a group of animals (n = 21) was euthanized and subjected to post-mortem analysis and bacteriological culture. Significant differences (p < .001) on the qualitative and quantitative (ODs) results were observed between herds using both serum and milk samples in the P22 ELISA. The correlation observed in the quantitative results obtained in serum and milk samples was very strong in animals from flock 2 (rs = 0.91) and moderate in animals from flock 1 (rs = 0.46). Among the slaughtered animals, the P22 ELISA detected a higher proportion of lesion-culture positive animals than cell-based diagnostic tests (61.9 and 66.7% using milk and serum samples, respectively). The P22 ELISA using milk samples demonstrated a similar sensitivity compared with serum samples, suggesting it might be a valuable test for TB control in dairy goats.Item Epidemiological factors associated with the exposure of cattle to Coxiella burnetii in the Madrid region of Spain(The Veterinary Journal, 2012) Álvarez Sánchez, Julio; Perez, A.; Mardones, F.O.; Pérez Sancho, Marta; García-Seco Romero, María Teresa; Pagés, E.; Mirat, F.; Díaz, R.; Carpintero, J.; Domínguez Rodríguez, Lucas JoséDomestic ruminants are considered to be the major source of Coxiella burnetii, the causative agent of Q fever. Even though Q fever is considered to be present worldwide, its distribution in many areas and countries remains unknown. Here, a serological assay was used to estimate the seroprevalence of C. burnetii in cattle in the Madrid region of Spain, to assess its spatial distribution, and to identify risk factors associated with positive results. Ten animals from each of 110 herds (n=1100) were randomly selected and analyzed using an ELISA test. In addition, epidemiological information, at both the herd and individual level, was collected. Variables for which an association with test results was detected in a bivariate analysis were included as predictors (main effects) in a multivariable logistic regression model. Herd and individual seroprevalences were 30% (95% CI=22.2-39.1) and 6.76% (95% CI=5.42-8.41), respectively, and a strong spatial dependence was identified at the first neighbour level using the Cuzick-Edwards test. Production type (dairy >beef >bullfighting) and age of animals (old vs. young) were the only variables significantly associated (P<0.05) with positive serological results at the herd and individual levels, respectively. These results indicate that cattle are exposed to C. burnetii in the Madrid region The high herd seroprevalence found in dairy herds (75%) indicates a higher risk of infection (probably for management reasons) whereas no C. burnetii positive bullfighting herds were identified.- Project number: 219
Item Infequus: plataforma de enfermedades infecciosas equinas(2019) Juan Ferré, Lucía De; Briones Dieste, Víctor; Bezos Garrido, Javier; Fores Jackson, Paloma; Camino Gutiérrez, Eliazar; Buendía Andrés, Aranzazu; Dorrego Rodríguez, Abel; Cruz López, Fátima; González Domínguez, Sergio; Romero Martínez, Beatriz; García Benzaquén, Nerea; Bárcena Asensio, Carmen; Mazariegos Martínez-Peñalver, María; Ancochea Nodal, Carlos; Hernández Carrillo, Javier; Pérez Sancho, Marta; Pérez Sancho, MartaDesarrollo de la herramienta de formación online Infequus, que ofrecerá a los alumnos y profesionales información actualizada sobre el diagnóstico y control de las enfermedades infecciosas en la especie equina. Item Neuropathological lesions in intravenous BCG-stimulated K18-hACE2 mice challenged with SARS-CoV-2(Veterinary Research, 2024) Sánchez Morales, Lidia; Porras, Néstor; García-Seco Romero, María Teresa; Pérez Sancho, Marta; Cruz López, Fátima; Chinchilla, Blanca; Barroso Arévalo, Sandra; Diaz Frutos, Marta; Buendía, Aránzazu; Moreno, Inmaculada; Briones Dieste, Víctor; Risalde, María de los Ángeles; de la Fuente, José; Juste, Ramón; Garrido, Joseba; Balseiro, Ana; Gortázar, Christian; Rodríguez Bertos, Antonio Manuel; Domínguez, Mercedes; Domínguez Rodríguez, Lucas JoséIn the wake of the COVID-19 pandemic caused by SARS-CoV-2, questions emerged about the potential effects of Bacillus Calmette-Guérin (BCG) vaccine on the immune response to SARS-CoV-2 infection, including the neurodegenerative diseases it may contribute to. To explore this, an experimental study was carried out in BCG-stimulated and non-stimulated k18-hACE2 mice challenged with SARS-CoV-2. Viral loads in tissues determined by RT-qPCR, histopathology in brain and lungs, immunohistochemical study in brain (IHC) as well as mortality rates, clinical signs and plasma inflammatory and coagulation biomarkers were assessed. Our results showed BCG-SARS-CoV-2 challenged mice presented higher viral loads in the brain and an increased frequency of neuroinvasion, with the greatest differences observed between groups at 3–4 days post-infection (dpi). Histopathological examination showed a higher severity of brain lesions in BCG-SARS-CoV-2 challenged mice, mainly consisting of neuroinflammation, increased glial cell population and neuronal degeneration, from 5 dpi onwards. This group also presented higher interstitial pneumonia and vascular thrombosis in lungs (3–4 dpi), BCG-SARS-CoV-2 mice showed higher values for TNF-α and D-dimer values, while iNOS values were higher in SARS-CoV-2 mice at 3–4 dpi. Results presented in this study indicate that BCG stimulation could have intensified the inflammatory and neurodegenerative lesions promoting virus neuroinvasion and dissemination in this experimental model. Although k18-hACE2 mice show higher hACE2 expression and neurodissemination, this study suggests that, although the benefits of BCG on enhancing heterologous protection against pathogens and tumour cells have been broadly demonstrated, potential adverse outcomes due to the non-specific effects of BCG should be considered.Item Short communication: Isolation frequency of bacteria causing lymphadenitis and abscesses in small ruminants in central Spain(Small Ruminant Research, 2017) Heras, Moisés de las; Torrijos, Carlos; Carrión Herrero, Francisco Javier; Fuente López, Ricardo De La; Díez De Tejada Martín, María Paloma; Pérez Sancho, Marta; Orden Gutiérrez, José Antonio; Domínguez Bernal, Gustavo RamónInfectious lymphadenitis in small ruminants caused by Corynebacterium pseudotuberculosis and Staphylococcus aureus subsp. anaerobius are widely distributed throughout the world, and result in significant economic losses. Trueperella pyogenes has also been associated with lymphadenitis in sheep and goats. In order to determinate the isolation frequency of the different agents associated with lymphadenitis and abscesses, 171 pus samples (135 from sheep and 36 from goats) from 46 flocks were investigated. Isolated bacteria were identified by MALDI-TOF method. S. aureus subsp. anaerobius was the most frequently detected agent. It was identified in 76 animals (59 sheep and 17 goats) from 24 of the surveyed flocks. Of these infected animal, 25 (32,9%) were over one year old, confirming that abscess disease may occur in a significant percentage of adult animals. C. pseudotuberculosis was identified in 45 of the sampled animals (36 sheep and 9 goats) from 24 flocks. Only 5 of animals suffering caseous lymphadenitis were under one year old. T. pyogenes was isolated from 17 animals (13 sheep and 4 goats) in 11 flocks. Seven of these samples were taken from subcutaneous abscesses located in not lymph nodes regions. A notable finding of this work is the isolation of Actinomyces hyovaginalis from 5 of the samples analyzed all of them taken from subcutaneous abscesses located in superficial lymph nodes regions. Thus, T. pyogenes and A. hyovaginalis should be included in the differential diagnosis of lymphadenitis in small ruminants. In 19 of the 46 surveyed flocks at least two of the four agents were detected, which underlines the need to analyze samples from several animals to reach an accurate diagnosis in flocks affected by lymphadenitis.Item First analysis by MALDI-TOF MS technique of Chryseobacteriumspecies relevant to aquaculture(Journal of fish diseases, 2017) Pérez Sancho, Marta; Vela Alonso, Ana Isabel; M Kostrzewa; L Zamora; Casamayor Sanz, Almudena; Domínguez Rodríguez, Lucas José; Fernández-Garayzábal Fernández, José FranciscoItem Active surveillance of antimicrobial resistance in companion animals: A pilot study in a Spanish Veterinary Teaching Hospital(Comparative Immunology, Microbiology and Infectious Diseases, 2024) Scarpellini, Raffaele; Leal Vélez de Mendizábal, Laura; Quevedo Caraballo, Sergio; Blanco Cancelo, José Luis; García Sánchez, Marta Eulalia; Pérez Sancho, Marta; Portero Fuentes, Miriam; Penelo Hidalgo, Silvia; Esposito, Erika; Mondo, Elisabetta; Piva, SilviaThe role of small animal veterinary hospitals in the onset and dissemination of antimicrobial-resistant organisms (AMROs) is still not clear, and the implementation of an internal surveillance systems is a cost-effective tool to better understand their impact. The aim of this study was to describe a pilot program of active surveillance in a Spanish Veterinary Teaching Hospital, developed to estimate the detection frequency of AMROs in the commensal flora of patients and in the environment. Surveillance was focused on Methicillin-resistant Staphylococci (MRS), third generation cephalosporins resistant gram-negative bacteria (3GCR-GNB), and carbapenems-resistant gram-negative bacteria (CR-GNB). Oral and perirectal swabs were collected in the same dogs and cats hospitalized > 48 h, at their admission and before their discharge. Out of 50 patients sampled, 24% (12/50) were carriers at admission of at least one of the three investigated AMROs. Twenty-eight percent of patients (14/50) acquired at least one AMRO during the hospital stay. MRS detection frequency at admission was 12% (6/50), while acquisition was 6% (3/50). 3GCR-GNB detection frequency was 14% at admission (7/50) and acquisition 22% (11/50), while CR-GNB detection frequency was 2% at admission (1/50) and acquisition 2% (1/50). Environmental surveillance (98 samples) showed a total detection frequency of 22.4% for MRS (22/98), 2% for 3GCR-GNB and CR-GNB (2/98). Clinical staff’ shoe soles showed high detection frequency for MRS (50%). 3GCR Escherichia coli was the most isolated species in patients (n = 17). The results show how active surveillance can be used as a tool to assess the impact of AMROs in veterinary hospitals to subsequently build up tailored control plans based on specific issues.Item First detection of Flavobacterium psychrophilum in juvenile Siberian sturgeons (Acipenser baerii) and description of the pathological findings(Journal of fish diseases, 2023) Chinchilla Rodríguez, Blanca; Vázquez Fernández, Esther; Rebollada Merino, Agustín Miguel; Pérez Sancho, Marta; Domínguez Rodríguez, Lucas José; Rodríguez Bertos, Antonio ManuelFlavobacterium psychrophilum affects many cultured fish species and is considered one of the most important bacterial pathogens causing substantial economic losses in salmonid aquaculture worldwide. Here, F. psychrophilum was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nested PCR as the aetiological agent causing mortality in diseased juvenile Siberian sturgeons (Acipenser baerii) reared on a freshwater fish farm. Diseased sturgeons were lethargic and displayed dark skin pigmentation, increased mucus production and the presence of skin ulcerations and haemorrhages specially on the ventral side and the base of fins. The histological examination of fish revealed proliferative branchitis, ulcerative and necrotizing dermatitis and myositis, lymphoid tissue atrophy, liver and kidney degeneration and thrombosis. To the best of our knowledge, this is the first report describing the infection of Siberian sturgeons by F. psychrophilum. The detection of F. psychrophilum in diseased Siberian sturgeons and the description of the pathological findings observed during the outbreak may contribute to a better understanding of the bacterium pathogenicity and the range of fish species susceptible to infection.Item MicroMundo: una aproximación a la perspectiva One Health de Salud Global mediante Aprendizaje-Servicio integrando diversos niveles educativos(2022) González Zorn, Bruno; Román González, Elvira; Molina Martín, María; Martín Brieva, Humberto; Escudero García-Calderón, José Antonio; Rodríguez Fernández, Carmina; Bezos Garrido, Javier; Álvarez Sánchez, Julio; Pérez Sancho, Marta; Amaro Torres, Francisco; Jimenez Cid, Victor; Suárez Rodríguez, Mónica; Patiño Alvarez, Belén; Calvo de Pablo, Pilar; Fernández-Acero Bascones, Teresa; Pavón Vergés, Mónica; Sanz Santamaría, Ana Belén; Díaz del Toro, Silvia; González Rubio, Gema; Vázquez Estévez, Covadonga; Gil Serna, Jéssica; Jiménez Gutiérrez, Elena; del Val Oriza, Elba; Valenti Sanguino, Marta; Sellers Moya, Ángela; Sastre Vergara, LucíaMicroMundo es la adaptación a Aprendizaje-Servicio (ApS) de la estrategia de crowdsourcing y ciencia ciudadana internacional Tiny Earth. Su objetivo de servicio esencial es acercar la cultura científica, la perspectiva One Health y la investigación biomédica a la sociedad, poniendo el foco en jóvenes estudiantes para fomentar la vocación por formación en Grados STEM y por la investigación. La pandemia de COVID-19 y sus consecuencias han puesto de manifiesto la urgencia de acercar la cultura científica en el ámbito de la Biomedicina y la Salud Pública a la sociedad e implicar en esta tarea a nuestros estudiantes. Pero también la pandemia ha impuesto cambios en nuestro esquema de trabajo durante este curso, lo que nos ha obligado a trabajar on-line en lugar de llevar a cabo las características actividades experimentales de aprendizaje activo en las que se fundamenta el proyecto en condiciones normales. Manteniendo los mismos objetivos, las intervenciones en los 20 Colegios e Institutos en los que hemos realizado el proyecto se han enfocado en la elaboración de materiales divulgativos e intervenciones en la comunidad por parte de los jóvenes estudiantes, de manera coordinada por nuestros estudiantes universitarios y la organización de un Simposio on-line de ámbito nacional donde se expusieron y compartieron las diversas iniciativas. La elevada participación en el Simposio, de inscripción gratuita (más de 500 inscritos) y la calidad de las 69 ponencias propuestas, la mayoría de manera conjunta por alumnos de instituto y universitarios, avala el enorme éxito de la iniciativa virtual y la consecución de objetivos. El material generado (videojuegos, entornos educativos virtuales, paisajes de aprendizaje, escape rooms, videos, campañas en Instagram o Tik Tok, blogs, etc) será muy valioso como material de apoyo en sucesivas ediciones de MicroMundo. Todo este material se irá divulgando en el portal www.esmisionposible.com las redes sociales @EsMisionPosible, gestionados por el proyecto. En resumen, consideramos que nuestro proyecto que implica tanto a estudiantes y profesores de centros educativos de Educación Secundaria y Bachillerato en la CAM a, como a profesores y estudiantes universitarios de los ámbitos de Sanidad Humana, Animal y Medioambiental de manera transversal e interfacultativa, ha afrontado con éxito el reto de adaptar el proyecto a la situación epidemiológica, reforzándolo con actividades no presenciales orientadas a consolidar una comunidad MicroMundo virtual en la que los estudiantes pueden compartir sus experiencias en la divulgación del problema de la resistencia y el fomento de la investigación para el descubrimiento de nuevos antimicrobianos.