Person:
Gutiérrez Cepeda, Luna

First Name

Luna

Last Name

Gutiérrez Cepeda

URI

https://hdl.handle.net/20.500.14352/80365

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Veterinaria

Department

Medicina y Cirugía Animal

Area

Medicina y Cirugía Animal

Identifiers

Full item page

Search Results

Now showing 1 - 10 of 13

Simple and economic colloidal centrifugation protocols may be incorporated into the clinical equine sperm processing procedure
(Animal Reproduction Science, 2011) Gutiérrez Cepeda, Luna; Fernández, A; Crespo Castejón, Francisco; Gosálvez Berenguer, Jaime; Serres Dalmau, María Consolacion
For many years in human assisted-reproduction procedures there have been special protocols to prepare and improve sperm quality. Colloidal centrifugation (CC) is a useful technique that has been proved to enhance semen quality by selection of the best spermatozoa for different species. Its use is recommended to improve fertility of subfertile stallions but current CC protocols are clinically complicated in the equine sperm processing technique due to economic and technical difficulties. The aim of this study was to determine the optimal processing procedures to adapt the use of a CC product (EquiPure™) in the equine reproduction industry. A total of nineteen ejaculates were collected from 10 Purebred Spanish Horses (P.R.E horses) using a Missouri artificial vagina. Gel-free semen aliquots were analyzed prior to treatment (control). Semen was subjected to one of six CC protocols with EquiPure™ and centrifuged samples were statistically evaluated by ANOVA and Duncan tests (p<0.05) for sperm quality and recovery rate. We obtained higher values by colloidal centrifugation in LIN, STR and BCF variables and DNA fragmentation index trended to be lower in most of the CC protocols. The studied protocols were shown to be as efficient in improving equine sperm quality as the current commercial EquiPure™, with the added advantage of being much more economical and simple to use. According to these results it seems to be possible to incorporate single layer and or high colloidal centrifugation volume protocols what would make them simple, economic and clinically viable for the equine sperm processing procedure.
Project number: 44
Pintando perros: estudio de la anatomía y biomecánica canina a través de la pintura sobre el animal vivo
(2017) Martínez Sainz, María Del Pilar; Gutiérrez Cepeda, Luna; Serres Dalmau, María Consolacion; Contreras Rodríguez, Julio; Hernandez Fernandez, Tatiana; Gonzalez Hernandez, Inmaculada; González Martínez, María Encina
Creación de un material docente (para web y plataformas móviles) pintando las estructuras anatómicas músculo-esqueléticas sobre perros vivos para el aprendizaje de la Anatomía funcional y Biomecánica canina de forma sencilla, visual y didáctica.
Optimización de las técnicas de acondicionamiento del semen equino para los procesos de conservación seminal
(2014) Gutiérrez Cepeda, Luna; Serres Dalmau, Consuelo
Desde hace muchos años en las técnicas de reproducción asistida en medicina humana existen protocolos de procesamiento de semen para incrementar su calidad. En la industria reproductiva equina, las técnicas de inseminación artificial se han desarrollado ampliamente, aunque el uso de semen criopreservado es menor que en otras especies debido a problemas de supervivencia espermática y variabilidad individual. Esta variabilidad individual también ha sido establecida entre razas. En general, sólo el 20-‐30% de los sementales producen un semen con buena capacidad de congelación, “buenos congeladores”, otro 40-‐ 60% aproximadamente con capacidad aceptable (aunque se ve afectado negativamente por la criopreservación) y otro 20-‐30% de sementales producen semen que no es congelable en absoluto, “malos congeladores”, Estas limitaciones determinan que la inseminación artificial con semen congelado en el équido esté menos extendida que en otras especies y encontrar sistemas y procedimientos que permitan incrementar el número de sementales y eyaculados adecuados para refrigeración y/o congelación es una de las metas principales. Unos de los procedimientos más extendidos para intentar mejorar la calidad seminal en equinos, especialmente en individuos tipificados como "malos refrigeradores-congeladores", es la eliminación de parte del plasma seminal mediante centrifugación simple. Sin embargo, esta técnica no ejerce tareas de selección, sino que concentra tanto los espermatozoides considerados normales, anormales y los no viables en el “pellet” resultante... [ABSTRACT]For many years in human assisted-‐reproduction procedures there have been special protocols to prepare and improve sperm quality. In the equine industry, artificial insemination techniques have been greatly developed, although cryopreserved semen is still less used comparing to others species due to spermatic survival problems and individual variability. This individual variability has also been established between breeds. In general, 20-‐30% of stallions are considered to have a good frozen-‐thawed sperm quality “Good Freezers”, 40-‐60% average frozen-‐thawed quality and the other 20-‐30% are not suitable for cryopreservation “Bad Freezers”. All these limitations determine that artificial insemination with frozen semen in the equine is less widespread than in other species and finding systems and procedures to increase the number of stallions and ejaculates suitable to be cooled and cryopreserved is and important goal. It has been suggested that equine sperm quality could be enhanced, particularly in stallions identified as “bad coolers/freezers”, by removing some of the seminal plasma by simple centrifugation. However, such procedure does not select the best spermatozoa as all sperm and debris are presented in the resulting pellet...
Comparative Kinematic Analysis of Hurdle Clearance Technique in Dogs: A Preliminary Report
(Animals, 2020) Miró, F.; López, P.; Vilar, J.M.; Galisteo, A.M.; Vivo, J.; Garrido-Castro, J.L.; Gutiérrez Cepeda, Luna
Although the jumping characteristics of agility dogs have been examined in recent years, there is currently a lack of data related to the suspension phase. The purpose of the present study was to investigate the biomechanics of the suspension phase of the agility jump and to analyze the kinematic differences in dogs with different jumping abilities. Two groups of dogs of the same height category (large dogs) competing at different skill levels and assessed as excellent jumpers (n = 4) and less-skilled jumpers (n = 3), respectively, were analyzed and statistically compared. Excellent jumpers showed longer and faster jumps with flatter jump trajectories than less-skilled jumpers. In less-skilled jumpers, the distance in front of the hurdle was notably greater than the distance behind it, while the difference between these two distances was less in excellent jumpers. Length and duration of the jump, maximal height of the jumping trajectory, take-off and landing distances to the hurdle, time of occurrence of maximal jump height, and time of change in back orientation essentially defines the suspension phase of the agility jump. This study presents preliminary evidence that the kinematic characteristics of hurdle clearance are different in excellent jumper dogs and in less-skilled jumper dogs.
Project number: 350
Pintando caballos: Estudio de la Anatomía y Biomecánica Equina a través de la Pintura sobre el Animal Vivo
(2016) Martínez Sainz, María Del Pilar; Rojo Salvador, Concepción; Contreras Rodríguez, Julio; Marín García, María Del Pilar; Serres Dalmau, María Consolacion; Fores Jackson, Paloma; Re, Michela Tatiana; Andres Alonso, Felipe; Gutiérrez Cepeda, Luna
Creación de un material docente (para web y plataformas móviles) pintando las estructuras anatómicas músculo-esqueléticas sobre caballos vivos para el aprendizaje de la anatomía funcional y biomecánica equina de forma sencilla, visual y didáctica.
Project number: 382
Elaboración de material docente para la enseñanza de la exploración física del caballo
(2022) Fores Jackson, Paloma; Gutiérrez Cepeda, Luna; Villaescusa Fernández, Alejandra; Villalba Orero, María; Ruiz de León Robledo, María de los Ángeles; Crespo Castejón, Francisco; Dominuez Gimbernat, Monica
El objetivo principal del presente proyecto fue incorporar una serie de recursos educativos virtuales que facilitaran e implementaran la enseñanza de la exploración física del caballo.
Evaluation of testicular echotexture with Ecotext as a diagnostic method of testicular dysfunction in stallions
(Theriogenology, 2022) Da Silva-Álvarez, E; Gaitskell-Phil, G; Ortiz-Rodríguez, JM; Serres Dalmau, María Consolacion; García-Rodríguez, B; Gutiérrez Cepeda, Luna; Martín-Cano, FE; Echegaray, A; Escartin-Casas, N; Requena, F; Gil, MC; Peña, FJ; Ortega-Ferrusola, C
This study aimed to assess if Ecotext, a new software for evaluation of testicular echotexture, is a good method for diagnosis of stallions with testicular dysfunction (TD). Relationships between Ecotext parameters and sperm motility and production, testicular volume, and testicular blood flow were also studied. Ecotext provides a total of six echotexture parameters: Ecotext 1 (black pixels), 2 (white pixels) and 3 (grey pixels), and another 3 parameters related to hypoechogenic areas: Ecotext tubular density (ETD), Ecotext tubular diameter (ETd), and Ecotext tubular area (ETA). Stallions (n = 33) were assessed using proven diagnostic techniques (spermiogram, B-mode and Pulse Doppler ultrasound), and subsequent analysis with Ecotext. Animals were classified as “control stallions” (n:21, acceptable semen quality), and “stallions with TD” (n:12, poor semen quality (TM < 60%, PM < 45% and total nº of sperm with PM < 2000 × 106 spz), that were subdivided into “induced TD group” (immunized, anti-GnRH vaccine) and “acquired TD group”. The acquired TD group showed differences in all Ecotext parameters in relation to controls (Ecotext 1:0.11 ± 0.17 vs 2.82 ± 2.52, Ecotext 2:1584.0 ± 575.8 vs 388 ± 368.2, Ecotext 3:134.2 ± 9.26; ETA: 2.14 ± 0.59 vs 5.40 ± 1.90; ETd: 65.66 ± 6.27 vs 86.93 ± 10.65 and ETD: 92.35 ± 11.24 vs 132.10 ± 16.35, p ≤ 0.001). Results suggest acquired TD stallions were suffering testicular degeneration with loss of architecture and function as all Ecotext parameters were altered in relation to controls. Induced TD horses only showed a reduction in ETD (116.2 ± 8.59 vs 132.10 ± 16.35, p ≤ 0.001), despite all sperm parameters being worse. These findings suggested immunized stallions probably only experience an acute loss of testicular functionality and parenchyma architecture is likely not affected since differences in Ecotext parameters with control stallions were not detected. ETD was the best parameter to identify animals with TD (AUC: 0.84, optimal cut-off value of 124.3 seminiferous tubules/cm2). Correlations were found between ETD and Doppler indices (PI: 0.60; RI: 0.47 p ≤ 0.001), total testicular volume (r: 0.48; p ≤ 0.05) and sperm motility (TM:0.51; and PM:0.54; p ≤ 0.001) and production (r:0.51; p ≤ 0.001). In summary, Ecotext could identify changes in testicular echotexture of stallions with TD. Results open the possibility for new research focused on establishing the relationship between Ecotext parameters and histomorphometry features in stallion testes.
Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation
(Animals (based), 2022) Bláquez Sarro, Juan Carlos; Gutiérrez Cepeda, Luna; Crespo Castejón, Francisco; Serres Dalmau, María Consolacion
The Purebred Spanish Horse, according to our clinical experience, is characterized by having a high number of stallions that do not meet the international commercial recommendations forequine-sperm cryopreservation. This means that artificial insemination with frozen semen from these stallions is less widespread than in other breeds. In this study, we investigated if the incorporation ofsingle-layer colloidal centrifugation prior to cryopreservation in clinical conditions could increase the number of ejaculates of Purebred Spanish stallions suitable for this processing, observing the influence of centrifugation and freezing extender protocol on post-thawed sperm motility. Using colloidal centrifugation, the percentage of ejaculates available to be frozen was increased from 35% (6/17) to71% (12/17), doubling the number of samples that could have been subjected to cryopreservation. We only found significant differences in linearity (LIN) and lateral head displacement (ALH) after5 min of incubation at 37 ◦C between colloidal and simple centrifugation processing techniques. No significant differences were found between the two different colloidal protocols in any of the variables considered. Colloidal centrifugation allowed us to obtain, from worse fresh-quality ejaculates, thawed sperm doses with similar quality to that of good-quality ejaculates. BotuCrio® produced, in general higher motility parameters and its characteristics than the other extenders analyzed, with significant differences found in comparison to Inra-Freeze® and Lac-Edta in both total (MOT) and progressive motility (PMOT) when using colloidal centrifugation and only in PMOT when applying simple centrifugation. Colloidal centrifugation optimized the efficiency of cryopreservation, as it allowed usto increase the number of ejaculates of Purebred Spanish Horses suitable to be frozen. Including these semen processing techniques in the freeze test could help to optimize equine-sperm cryopreservation protocols, especially when dealing with individuals or breeds for which initially low sperm quality prevents or limits their inclusion in sperm cryopreservation programs
Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen
(Animals, 2024) Zabala Argüelles, Sonsoles Mercedes; Serres Dalmau, María Consolacion; Montero Serra, Natalia; Crespo Castejón, Francisco; Lorenzo González, Pedro Luis; Pérez-Aguilera, Verónica; Galán López, Carmen; Domínguez Gimbernat, Mónica; Oliet Palá, Agustín; Moreno, Santiago; González Zorn, Bruno; Gutiérrez Cepeda, Luna
The study assessed the impact of four equine semen processing techniques on sperm quality and microbial load immediately post-processing and after 48 h of refrigeration. The aim was to explore the potential reduction of prophylactic antibiotic usage in semen extenders. Semen from ten adult stallions was collected and processed under a strict hygiene protocol and divided into four aliquots: Simple Centrifugation with antibiotics (SC+), Simple Centrifugation (SC−), Single-Layer Colloidal Centrifugation (CC−), and Filtration (with SpermFilter®) (F−), all in extenders without antibiotics. Sperm motility, viability, and microbial load on three culture media were assessed. No significant differences were observed in the main in the sperm quality parameters among the four protocols post-processing and at 48 h (p < 0.05 or p < 0.1). Microbial loads in Columbia 5% Sheep Blood Agar and Schaedler vitamin K1 5% Sheep Blood Agar mediums were significantly higher (p < 0.10) for raw semen than for CS+, CC−, and F− post-processing. For Sabouraud Dextrose Agar medium, the microbial load was significantly higher (p < 0.10) in raw semen compared to CS+ and F−. No significant differences (p < 0.10) were found in 48 h chilled samples. Regardless of antibiotic presence, the evaluated processing methods, when combined with rigorous hygiene measures, maintained semen quality and reduced microbial load to the same extent as a traditional protocol using antibiotics.
Project number: 376
Creación de un entorno de enseñanza online para los programas de posgrado
(2016) Lorenzo González, Pedro Luis; Fernández Álvarez, Manuela; Olmeda García, Ángeles Sonia; Serres Dalmau, María Consolacion; Gutiérrez Cepeda, Luna; Manzano Barea, Elena
En este proyecto se ha conseguido modernizar, a través de las diferentes labores y actividades desarrolladas, las herramientas de aprendizaje a través del uso de medios en formato online que facilitan la comprensión y adquisición de conocimientos. Se ha logrado introducir un método alternativo a la enseñanza tradicional, basado en el aprendizaje online con apoyo de material fácilmente accesible en web, lo que da la oportunidad a los estudiantes, antiguos alumnos y profesorado de mejorar sus conocimientos, mantenerse actualizados y administrarse sus tiempos de trabajo y estudio. Las herramientas utilizadas han logrado incrementar el número de alumnos matriculados. Este curso académico 2016-2017, el título propio de Experto en Bases de la Fisioterapia y Rehabilitación Animal, cuenta con el mayor número de matriculaciones desde su inicio en 2012. Además, se ha incrementado el acceso de alumnos extranjeros comunitarios y extracomunitarios, lo que demuestra el alcance de las labores realizadas. Se ha logrado crear una herramienta que favorece la cooperación interdisciplinar en el intercambio de conocimientos entre los diferentes programas de formación de posgrado y entre las diferentes ediciones de los mismos. Se han creado herramientas que han facilitado el uso del inglés así como las interpelaciones de los alumnos entre si y con los diferentes profesores de la formación solventando las barreras geográficas y creando una comunidad formativa e interactiva de gran utilidad.