Person: Sánchez-Paniagua López, Marta
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First Name
Marta
Last Name
Sánchez-Paniagua López
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Analítica
Identifiers
17 results
Search Results
Now showing 1 - 10 of 17
Item Electrochemical genosensor for Klotho detection based on aliphatic and aromatic thiols self-assembled monolayers(Talanta, 2020) Sánchez-Paniagua López, Marta; Palenzuela Batista, Sara; Manzanares Palenzuela, Lorena; López Ruiz, María BeatrizChanges in expression of Klotho gene are associated with chronic kidney disease and its potential as early biomarker is being studied. We report, for the first time, the detection of Klotho gene by a biosensor platform. Self-assembled mixed monolayers (SAMs) as DNA immobilization method in screen-printed gold electrodes and a sandwich format detection were used in the development of an electrochemical genosensor for the detection of a 100-mer DNA fragment, copy of the partial region of the mRNA Klotho gene. The use of different binary and ternary SAMs based on aliphatic (mercaptohexanol, MCH, and hexanedithiol, HDT) and aromatic (mercaptophenylacetic acid, MPAA) thiol diluents and capture probe (CP) as sensing phases was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. Multiple configurations were studied, changing the order of component addition and comparing co-immobilization and two-step immobilization processes. The procedure for binary SAM preparation consisting of sequential addition of a thiol diluent followed by CP was found to have the least detrimental impact on electrochemical performance. The signal-to-blank ratios increased considerably in the case of thioaromatic binary DNA monolayers, MPPA/CP, compared to the values obtained for aliphatic SAMs. Ternary monolayers formed by MCH and HDT rendered good fractional coverage levels and generated more reversible redox reactions at the surface, mostly when CP was firstly immobilized, CP/HDT/MCH. A significant reduction of the blank and non-specific (non-complementary sequence) signals was obtained with this ternary SAM, compared to binary SAMs and an increase of 2.42-fold of the S/B ratio (10 nM of target) compared with MPAA/CP SAMs. A linear response in the range of 5·10−10 to 5·10−8 M was obtained with CP/HDT/MCH monolayer, with a detection limit of 0.5 nM and RSD of 8.10%.- Project number: 185
Item ¿Y si nos escapamos de manera virtual? Hacia la virtualización de las "escape rooms"(2021) Mateos-Aparicio Cediel, Inmaculada; Sánchez-Paniagua López, Marta; López Ruiz, María Beatriz; Rueda Rodríguez, María del Carmen; García Alonso, María Alejandra; Molina Santos, Marina Mercedes; Moreno Guzmán, María; Pérez Rodríguez, María Luisa; Raggio Quilez, José; Sevilla Sierra, María Paz; González Aguilera, Guillermo; Gonzalez de Toro, Teresa Almudena; Jiménez de la Peña, Rocío Teresa Item Fast and sensitive diagnosis of autoimmune disorders through amperometric biosensing of serum anti-dsDNA autoantibodies(Biosensors & Bioelectronics, 2020) López Ruíz, Beatriz ; Arévalo Pérez, Beatriz; Serafín González-Carrato, Verónica; Sánchez-Paniagua López, Marta; Montero Calle, Ana; Barderas Manchado, Rodrigo; López Ruiz, María Beatriz; Campuzano Ruiz, Susana; Yáñez-Sedeño Orive, Paloma; Pingarrón Carrazón, José ManuelThis work reports the first amperometric biosensor involving the use of neutravidin-functionalized magnetic microbeads (NA-MBs) modified with a biotinylated-anti-dsDNA (b-dsDNA) as efficient magnetic microcarriers to selectively capture anti-dsDNA autoantibodies (IgG, IgA and IgM AAbs) present in the sera of patients with rheumatoid arthritis (RA). Subsequently, the attached anti-dsDNA AAbs are detected with a mixture of conventional HRP-labeled secondary antibodies (HRP-anti-human IgG/IgM/IgA mixture). The biorecognition event is monitored by amperometric transduction using the hydroquinone (HQ)/H2O2 system upon capturing the modified MBs on the surface of screen-printed carbon electrodes (SPCEs). The developed bioplatform exhibits a linear calibration plot ranging from 1 to 200 IU mL−1 with a LOD of 0.3 IU mL−1 for anti-dsDNA AAbs standards. In addition, the biosensor allows performing the determination of the anti-dsDNA AAbs levels directly in 100-times diluted serum samples from patients diagnosed with RA and in just 75 min. The obtained results are in agreement with those provided by an ELISA kit and allow discrimination between positive and negative samples.Item Edible plant by-products as source of polyphenols: prebiotic effect and analytical methods(Critical Reviews in Food Science and Nutrition, 2022) García Alonso, María Alejandra; Sánchez-Paniagua López, Marta; Manzanares-Palenzuela, C. Lorena; Redondo Cuenca, Araceli; López Ruiz, María BeatrizPolyphenols with high chemical diversity are present in vegetables both in the edible parts and by-products. A large proportion of them remains unabsorbed along the gastrointestinal tract, being accumulated in the colon, where they are metabolized by the intestinal microbiota. These polyphenols have been found to have “prebiotic-like” effects. The edible plant industry generates tons of residues called by-products, which consist of unutilized plant tissues (peels, husks, calyxes and seeds). Their disposal requires special and costly treatments to avoid environmental complications. Reintroducing these by-products into the value chain using technological and biotechnological practices is highly appealing since many of them contain nutrients and bioactive compounds, such as polyphenols, with many health-promoting properties. Edible plant by-products as a source of polyphenols highlights the need for analytical methods. Analytical methods are becoming increasingly selective, sensitive and precise, but the great breakthrough lies in the pretreatment of the sample and in particular in the extraction methods. This review shows the importance of edible plant by-products as a source of polyphenols, due to their prebiotic effect, and to compile the most appropriate analytical methods for the determination of the total content of phenolic compounds as well as the detection and quantification of individual polyphenols.- Project number: 5
Item Estrategias metodológicas enfocadas a la mejora del aprendizaje en estudiantes con diversidad(2018) Sánchez-Paniagua López, Marta; López Ruiz, Beatriz; Sevilla Sierra, María Paz; Rueda Rodríguez, María Del Carmen; Molina Santos, Marina Mercedes; Mateos-Aparicio Cediel, Inmaculada; Redondo Cuenca, Araceli; Tenorio Sanz, María Dolores; Rodrigo Martín, Rosa; Aragoneses Velasco, Laura; López Salvador, Alba; Cervero Frías, Verónica; Urquía Fermosell, CarlosDiseño y desarrollo de estrategias metodológicas que faciliten el aprendizaje de los estudiantes en general y que permitan a los estudiantes con diversidad funcional la integración y participación en el proceso de aprendizaje, que refuercen su inclusión y que favorezcan la adquisición de competencias. Item Biosensores amperométricos de tirosinasa para la determinación de compuestos fenólicos en medios acuosos y no acuosos(2009) Sánchez-Paniagua López, Marta; López Ruiz, Beatriz; López Cabarcos, EnriqueItem Serum autoantibody biomarkers for management of rheumatoid arthritis disease(Biosensors, 2023) Sánchez Tirado, Esther; Agüí Chicharro, María Lourdes; Sánchez-Paniagua López, Marta; González Cortés, Araceli; López Ruiz, María Beatriz; Yáñez-Sedeño Orive, Paloma; Pingarrón Carrazón, José ManuelRheumatoid arthritis (RA) is a systemic chronic autoimmune inflammatory disease that is characterized by the destruction of bone and production of autoantibodies such as rheumatoid factor (RF) and anticitrullinated protein antibodies (ACPAs). The high prevalence of this disease and the need of affordable tools for its early detection led us to prepare the first electrochemical immunoplatform for the simultaneous determination of four RA biomarkers, the autoantibodies: RF, anti-peptidyl-arginine deiminase enzyme (anti-PAD4), anti-cyclic citrullinated peptide (anti-CCP), and anti-citrullinated vimentin (anti-MCV). Functionalized magnetic beads (MBs) were used to immobilize the specific antigens, and sandwich-type immunoassays were implemented for the amperometric detection of the four autoantibodies, using the horseradish peroxidase (HRP)/H2O2/hydroquinone (HQ) system. The immunoplatform was applied to the determination of the biomarkers in human serum of twenty-two patients diagnosed with RA and four healthy individuals, and the results were validated against ELISA tests and the certified values.Item The Pharmacological Activity of Camellia sinensis (L.) Kuntze on Metabolic and Endocrine Disorders: A Systematic Review(Biomolecules, 2020) Sánchez-Paniagua López, Marta; González Burgos, Elena María; Iglesias Peinado, Irene; Lozano Fernández, Rafael; Gómez-Serranillos Cuadrado, María PilarTea made from Camellia sinensis leaves is one of the most consumed beverages worldwide. This systematic review aims to update Camellia sinensis pharmacological activity on metabolic and endocrine disorders. Inclusion criteria were preclinical and clinical studies of tea extracts and isolated compounds on osteoporosis, hypertension, diabetes, metabolic syndrome, hypercholesterolemia, and obesity written in English between 2014 and 2019 and published in Pubmed, Science Direct, and Scopus. From a total of 1384 studies, 80 reports met inclusion criteria. Most papers were published in 2015 (29.3%) and 2017 (20.6%), conducted in China (28.75%), US (12.5%), and South Korea (10%) and carried out with extracts (67.5%, especially green tea) and isolated compounds (41.25%, especially epigallocatechin gallate). Most pharmacological studies were in vitro and in vivo studies focused on diabetes and obesity. Clinical trials, although they have demonstrated promising results, are very limited. Future research should be aimed at providing more clinical evidence on less studied pathologies such as osteoporosis, hypertension, and metabolic syndrome. Given the close relationship among all endocrine disorders, it would be of interest to find a standard dose of tea or their bioactive constituents that would be beneficial for all of them.- Project number: 263
Item Diseño y aplicación de actividades virtuales. Análisis de datos de aprendizaje relacionados con la utilización de dichas actividades (learning analytics)(2015) López Ruíz, Beatriz; Frutos Cabanillas, Gloria; Rueda Rodríguez, María del Carmen; Sevilla Sierra, María Paz; Sainz Villanueva, María Cristina; Sánchez-Paniagua López, Marta Item Biosensors for GMO Testing: Nearly 25 Years of Research(2018) Sánchez-Paniagua López, Marta; Manzanares Palenzuela, Carmen Lorena ; López Ruiz, María BeatrizIn the nearly two decades since genetically modified organisms (GMOs) were first commercialized, genetically engineered crops have gained ground on their conventional counterparts, reaching 185 million hectares worldwide in 2016. The technology has bestowed most of its benefits on enhancing crop productivity with two main traits currently dominating the market: insect-resistant and herbicide-tolerant crops. Despite their rapid and vast adoption by farmers worldwide, GMOs have generated heated debates, especially in European countries (EU), driven mostly by consumers concerned about safety of transgenic foods and about the potential impact on the environment. The need to monitor and to verify the presence and the amount of GMOs in agricultural crops and in food products has generated interest in analytical methods for sensitive, accurate, rapid, and cheap detection of these products. DNA biosensors have been envisioned as a novel DNA-detection technology that would one day substitute current amplification-based methods, providing hand-held, quick, and ultrasensitive gene-level detection. This review summarizes the contributions made in nearly 20 years of research regarding the application of genosensing technology for the qualitative and quantitative determination of transgenic traits.