Person: Heras Polo, Beatriz De Las
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First Name
Beatriz De Las
Last Name
Heras Polo
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Farmacología, Farmacognosia y Botánica
Area
Farmacología
Identifiers
18 results
Search Results
Now showing 1 - 10 of 18
- Project number: 333
Item Implementación de la experiencia de un congreso virtual para estudiantes de Farmacología, Farmacognosia y Fitoterapia como herramienta de aprendizaje, emprendimiento, estímulo del trabajo colaborativo y autoevaluación(2015) Ortega Hernández-Agero, María Teresa; Carretero Accame, María Emilia; Benedí González, Juana María; Gómez-Serranillos Cuadrado, María Pilar; Heras Polo, Beatriz De Las; Palomino Ruiz-Poveda, Olga María; García Diz, Luis Item Campaña de concienciación para el uso racional de fármacos ansiolíticos e hipnóticos(2023) Cuadrado Berrocal, Irene; Prieto Chinchilla, Patricia; Ortega Hernández-Agero, María Teresa; Heras Polo, Beatriz De Las; Palomino Ruiz-Poveda, Olga María; González Cofrade, Laura- Project number: 167
Item Diseño, elaboración y difusión de una campaña de concienciación ciudadana sobre fármacos que afectan a la conducción de vehículos(2020) Cuadrado Berrocal, Irene; Heras Polo, Beatriz De Las; Ortega Hernández-Agero, María Teresa; Palomino Ruiz-Poveda, Olga María; González Cofrade, LauraProyecto Aprendizaje Servicio para el diseño, elaboración y difusión de una campaña de sensibilización ciudadana, realizada por estudiantes de Farmacia, sobre el uso de fármacos y la conducción de vehículos, basada en las necesidades de la sociedad. Item Amphiphilic Acrylic Nanoparticles Containing the Poloxamer Star Bayfit® 10WF15 as Ophthalmic Drug Carriers(Polymers, 2019) Gómez Ballesteros, Miguel; Andrés Guerrero, Vanesa; Parra Luna, Francisco; Marinich, Jorge; Heras Polo, Beatriz De Las; Molina Martínez, Irene Teresa; Vázquez Lasa, Blanca; San Román Del Barrio, Julio; Herrero Vanrell, María Del RocíoTopical application of drops containing ocular drugs is the preferred non-invasive route to treat diseases that a_ect the anterior segment of the eye. However, the formulation of eye drops is a major challenge for pharmacists since the access of drugs to ocular tissues is restricted by several barriers. Acetazolamide (ACZ) is a carbonic anhydrase inhibitor used orally for the treatment of ocular hypertension in glaucoma. However, large ACZ doses are needed which results in systemic side e_ects. Recently, we synthesized copolymers based on 2-hydroxyethyl methacrylate (HEMA) and a functionalized three-arm poloxamer star (Bayfit-MA). The new material (HEMA/Bayfit-MA) was engineered to be transformed into nanoparticles without the use of surfactants, which represents a significant step forward in developing new ophthalmic drug delivery platforms. Acetazolamide-loaded nanocarriers (ACZ-NPs) were prepared via dialysis (224 +/- 19 nm, ̶ 17.2 +/- 0.4 mV). The in vitro release rate of ACZ was constant over 24 h (cumulative delivery of ACZ: 83.3 +/- 8.4%). Following standard specifications, ACZ-NPs were not cytotoxic in vitro in cornea, conjunctiva, and macrophages. In normotensive rabbits, ACZ-NPs generated a significant intraocular pressure reduction compared to a conventional solution of ACZ (16.4% versus 9.6%) with the same dose of the hypotensive drug (20 μg). In comparison to previously reported studies, this formulation reduced intraocular pressure with a lower dose of ACZ. In summary, HEMA:Bayfit-MA nanoparticles may be a promising system for ocular topical treatments, showing an enhanced ocular bioavailability of ACZ after a single instillation on the ocular surface.Item Labdane conjugates protect cardiomyocytes from doxorubicin‐induced cardiotoxicity(Drug Development Research, 2022) Cuadrado Berrocal, Irene; Oramas Royo, Sandra; González Cofrade, Laura; Amesty, Ángel; Hortelano, Sonsoles; Estévez Braun, Ana; Heras Polo, Beatriz De LasThe cardiovascular side effects associated with doxorubicin (DOX), a wide spectrum anticancer drug, have limited its clinical application. Therefore, to explore novel strategies with cardioprotective effects, a series of new labdane conjugates were prepared (6a–6c and 8a–8d) from the natural diterpene labdanodiol (1). These hybrid compounds contain anti‐inflammatory privileged structures such as naphthalimide, naphthoquinone, and furanonaphthoquinone. Biological activity of these conjugates against DOX‐induced cardiotoxicity was tested in vitro and the potential molecular mechanisms of protective effects were explored in H9c2 cardiomyocytes. Three compounds 6c, 8a, and 8b significantly improved cardiomyocyte survival, via inhibition of reactive oxygen species‐mediated mitogen‐activated protein kinase signaling pathways (extracellular signal‐regulated kinase and c‐Jun N‐terminal kinase) and autophagy mediated by Akt activation. Some structure–activity relationships were outlined, and the best activity was achieved with the labdane–furonaphthoquinone conjugate 8a having an N‐cyclohexyl substituent. The findings of this study pave the way for further investigations to obtain more compounds with potential cardioprotective activity.- Project number: 145
Item UCMspace-comunica)))), un espacio web como herramienta de aprendizaje de carácter multidisciplinar para la mejora de la comunicación científica(2017) Ortega Hernández-Agero, María Teresa; Torres Muñoz, Margarita; Benedí González, Juana María; Raposo González, Rafaela; Heras Polo, Beatriz De Las; Palomino Ruiz-Poveda, Olga María; Martín Gómez, María Soledad; Pintado Valverde, Ana; Sánchez de Rojas Fernández-Cabrera, Ramón; Castilla Rodriguez, Carlos María; Garcimartín Álvarez, AlbaDiseño, ejecución y evaluación de un espacio web (UCMspace-comunica)))), que pueda ser utilizado como herramienta de aprendizaje eficaz, de carácter multidisciplinar, para la correcta comunicación de los contenidos específicos adquiridos por los alumnos en las diferentes materias que integran los grados universitarios, incluyendo el Trabajo Fin de Grado. Esta herramienta puede ser útil para optimizar la labor tutorial del profesor y el desarrollo profesional de los futuros farmacéuticos. Item Pharmacological modification of endogenous antioxidant enzymes by ursolic acid on tetrachloride-induced liver damagein rats and primary cultures of rat hepatocytes(Experimental and Toxicologic Pathology, 2001) Martín-Aragón Álvarez, Sagrario; Heras Polo, Beatriz De Las; Sánchez Reus, María Isabel; Benedí González, Juana MaríaThe purpose of this study was to investigate possible protective effects of ursolic acid against CCl4-induced alterations of antioxidant defence enzymes in vivo as well as its effects against CCl4-intoxication in vitro. Pre-treatment of rats with ursolic acid significantly reduced serum levels of glutamate-oxalate-transaminase and glutamate-pyruvate-transaminase previously increased by administration of CCl4. Treatment with ursolic acid also significantly reversed the decreased superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase activities and glutathione levels in the liver, as the concentration of reduced glutathione was increased and the content of oxidized glutathione decreased in ursolic acid treated groups. Levels of lipid peroxidation were higher in the CCl4 group but the increase was also reduced after drug treatment (p < 0.01 for 1, 2.5 and 5 mmol/kg). In vitro results indicated that addition to the culture medium of ursolic acid (p < 0.01 for 500 microM) resulted in a reduction of glutamate-oxalate-transaminase, lactate dehydrogenase activities and in a good survival rate for the CCl4-intoxicated hepatocytes. Ursolic acid also ameliorated lipid peroxidation in primary cultured rat hepatocytes exposed to CCl4, as demonstrated by a reduction in malondialdehyde production. Moreover, ursolic acid (50-500 microM) showed radical scavenging properties in terms of hydroxyl formation. The results obtained suggest that ursolic acid treatment can normalize the disturbed antioxidant status of rats intoxicated with CCl4 by maintaining the levels of glutathione and by inhibiting the production of malondialdehyde due to its radical scavenging properties.Item Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film(Polymers, 2018) Vicario De La Torre, Marta; Caballo González, María; Vico Ruiz, Eva; Morales Fernández, Laura; Arriola Villalobos, Pedro; Heras Polo, Beatriz De Las; Benítez Del Castillo Sánchez, José Manuel; Guzmán Navarro, Manuel; Millar, Thomas; Herrero Vanrell, María Del Rocío; Molina Martínez, Irene TeresaDry eye is commonly treated with artificial tears; however, developing artificial tears similar to natural tears is difficult due to the complex nature of tears. We characterized and evaluated a novel artificial tear formulation with components similar to the lipid and aqueous constituents of natural tears. Nano-liposomes, composed in part of phosphatidylcholine, were dispersed in an aqueous solution of bioadhesive sodium hyaluronate. Liposome size, zeta potential, and physicochemical properties of the fresh and stored (4°C) liposomal formulation were analyzed. In vitro tolerance was tested using human corneal and conjunctival cell lines by exposures of 15 min to 4 h. The tolerance of the liposomal formulation was evaluated in animals (rabbits). The average liposome size was 186.3 +/- 7.0 nm, and the zeta potential was negative. The osmolarity of the formulation was 198.6 +/- 1.7 mOsm, with a surface tension of 36.5 +/- 0.4 mN/m and viscosity of 3.05 +/- 0.02 mPa·s. Viability values in the human corneal and conjunctival cell lines were always >80%, even after liposomal formulation storage for 8 weeks. Discomfort and clinical signs after instillation in rabbit eyes were absent. The new formulation, based on phosphatidylcholine-liposomes dispersed in sodium hyaluronate has suitable components and characteristics, including high in vitro cell viability and good in vivo tolerance, to serve as a tear substitute.Item The Use of Mucoadhesive Polymers to Enhance the Hypotensive Effect of a Melatonin Analogue, 5-MCA-NAT, in Rabbit Eyes(Investigative ophthalmology & visual science, 2011) Andrés Guerrero, Vanesa; Molina Martínez, Irene Teresa; Peral Cerda, María Asunción; Heras Polo, Beatriz De Las; Pintor Just, Jesús Jerónimo; Herrero Vanrell, María Del RocíoPurpose.: 5-Methoxy-carbonylamino-N-acetyltryptamine (5-MCA-NAT, a melatonin receptor agonist) produces a clear intraocular pressure (IOP) reduction in New Zealand White rabbits and glaucomatous monkeys. The goal of this study was to evaluate whether the hypotensive effect of 5-MCA-NAT was enhanced by the presence of cellulose derivatives, some of them with bioadhesive properties, as well as to determine whether these formulations were well tolerated by the ocular surface. Methods.: Formulations were prepared with propylene glycol (0.275%), carboxymethyl cellulose (CMC, 0.5% and 1.0%) of low and medium viscosity and hydroxypropylmethyl cellulose (0.3%). Quantification of 5-MCA-NAT (100 μM) was assessed by HPLC. In vitro tolerance was evaluated by the MTT method in human corneal-limbal epithelial cells and normal human conjunctival cells. In vivo tolerance was analyzed by biomicroscopy and specular microscopy in rabbit eyes. The ocular hypotensive effect was evaluated measuring IOP for 8 hours in rabbit eyes. Results.: All the formulations demonstrated good in vitro and in vivo tolerance. 5-MCA-NAT in CMC medium viscosity 0.5% was the most effective at reducing IOP (maximum IOP reduction, 30.27%), and its effect lasted approximately 7 hours. Conclusions.: The hypotensive effect of 5-MCA-NAT was increased by using bioadhesive polymers in formulations that are suitable for the ocular surface and also protective of the eye in long-term therapies. The use of 5-MCA-NAT combined with bioadhesive polymers is a good strategy in the treatment of ocular hypertension and glaucoma.- Project number: 247
Item Atlas interactivo de Fitoterapia(2022) Palomino Ruiz-Poveda, Olga María; Cuadrado Berrocal, Irene; Estrada San Antolín, Pablo; González Cofrade, Laura; González Fuentes, Adrián; Heras Polo, Beatriz De Las; Prieto Chinchilla, Patricia; Ortega Hernández-Agero, María TeresaEste proyecto pretende crear un Atlas de Fitoterapia actualizado, mediado por recursos digitales y multimedia; elaborado por un equipo interdisciplinar y colaborativo, capaz de mejorar la enseñanza y aprendizaje de los estudiantes de farmacia y la adquisición de habilidades para su desarrollo profesional futuro.