Person: Torrado Durán, Juan José
Loading...
First Name
Juan José
Last Name
Torrado Durán
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Farmacia Galénica y Tecnología Alimentaria
Area
Farmacia y Tecnología Farmaceútica
Identifiers
20 results
Search Results
Now showing 1 - 10 of 20
Item Antifungal and Antiparasitic Drug Delivery(Pharmaceutics, 2020) Torrado Durán, Juan José; Serrano López, Dolores Remedios; Capilla, JavierFungal and parasitic diseases affect more than a billion people across the globe, one-sixth of the world’s population, mostly located in developing countries. The lack of effective and safer treatments combined with a deficient diagnosis lead to serious chronic illness or even death. There is a mismatch between the rate of drug resistance and the development of new medicines. Formulation of antifungal and antiparasitic drugs adapted to different administration routes is challenging, bearing in mind their poor water solubility, which limits their bioavailability and efficacy. Hence, there is an unmet clinical need to develop vaccines and novel formulations and drug delivery strategies that can improve the bioavailability and therapeutic effect by enhancing their dissolution, increasing their chemical potency, stabilising the drug and targeting high concentration of drug to the infection sites. This Editorial regards the ten research contributions presented in the Special Issue “Antifungal and Antiparasitic Drug Delivery”.Item Evaluating the Potential of Ursolic Acid as Bioproduct for Cutaneous and Visceral Leishmaniasis(Molecules, 2020) Bilbao Ramos, Pablo Estanislao; Serrano López, Dolores Remedios; Ruiz Saldaña, Helga Karina; Torrado Durán, Juan José; Bolás Fernández, Francisco; Dea Ayuela, María AuxiliadoraLeishmaniasis affects around 12 million people worldwide and is estimated to cause the ninth-largest disease burden. There are three main forms of the disease, visceral (VL), cutaneous (CL), and mucocutaneous (MCL), leading to more than one million new cases every year and several thousand deaths. Current treatments based on chemically synthesized molecules are far from ideal. In this study, we have tested the in vitro and in vivo efficacy of ursolic acid (UA), a multifunctional triterpenoid with well-known antitumoral, antioxidant, and antimicrobial effects on different Leishmania strains. The in vitro antileishmanial activity against the intracellular forms was six and three-fold higher compared to extracellular forms of L. amazonensis and L. infantum, respectively. UA also showed to be a potent antileishmanial drug against both VL and CL manifestations of the disease in experimental models. UA parenterally administered at 5 mg/kg for seven days significantly reduced the parasite burden in liver and spleen not only in murine acute infection but also in a chronic-infection model against L. infantum. In addition, UA ointment (0.2%) topically administered for four weeks diminished (50%) lesion size progression in a chronic infection model of CL caused by L. amazonensis, which was much greater than the effect of UA formulated as an O/W emulsion. UA played a key role in the immunological response modulating the Th1 response. The exposure of Leishmania-infected macrophages to UA led to a significant different production in the cytokine levels depending on the Leishmania strain causing the infection. In conclusion, UA can be a promising therapy against both CL and VL.- Project number: 254
Item Creación de nuevo recurso educativo virtual para estudiantes de grado en Farmacia(2020) Torrado Durán, Susana; Ballesteros Papantonakis, María de la Paloma; Torrado Durán, Juan José; Torre Iglesias, Paloma Marina de la; Torrado Durán, Santiago; Álvarez Álvarez, Covadonga; Martínez Caballero, María Aranzazu; Martínez Caballero, Marta; Torrado Durán, Guillermo; Serrano López, Dolores Remedios; Ares Lombán, Irma; Torrado Salmerón, Carlos Felix; Rodríguez Torrado, Marta; Fernández Gutiérrez, Jesús Miguel; Rodríguez Torrado, David; Notivoli Díez, Pablo; Franco Gil, María Elvira - Project number: 60
Item Creación de nuevos recursos educativos virtuales para estudiantes de grado en Farmacia(2022) Torrado Durán, Susana; Ballesteros Papantonakis, Paloma; Torrado Durán, Juan José; Álvarez Álvarez, Covadonga; Torrado Durán, Santiago; Martínez Caballero, Marta; Torrado Durán, Guillermo; Martínez Caballero, María Aranzazu; Franco Gil, Elvira; Sastre Barbas, Almudena; Rodríguez Torrado, Marta; Torrado Salmerón, Carlos Félix; De la Torre Iglesias, Paloma; Rodríguez Torrado, David; Ares Lombán, Irma; Fernández Gutierrez, Jesús Miguel Item Tuning the Transdermal Delivery of Hydroquinone upon Formulation with Novel Permeation Enhancers(Pharmaceutics, 2019) Serrano, Dolores R.; Gordo, María José; Matji, Antonio; González, Salvador; Lalatsa, Aikaterini; Torrado Durán, Juan JoséHydroquinone (HQ) is an anti-hyperpigmentation agent with poor physicochemical stability. HQ formulations are currently elaborated by compounding in local pharmacies. Variability in the characteristics of HQ topical formulations can lead to remarkable di_erences in terms of their stability, e_cacy, and toxicity. Four di_erent semisolid O/Wformulations with 5% HQ were prepared using: (i) Beeler´s base plus antioxidants (F1), (ii) Beeler´s base and dimethyl isosorbide (DMI) as solubiliser (F2), (iii) olive oil and DMI (F3), and (iv) Nourivan®, a skin-moisturising and antioxidant base, along with DMI (F4). Amongst the four formulations, F3 showed the greatest physicochemical stability with less tendency to coalescence but with marked chromatic aberrations. An inverse correlation was established by multivariate analysis between the mean droplet size in volume and the steady-state flux, which explains why F3, with the smallest droplet size and the most hydrophobic excipients, exhibited the highest permeation across both types of membranes with enhancement ratios of 2.26 and 5.67-fold across Strat-M® and mouse skin, respectively, compared to F1. It is crucial to understand how the HQ is formulated, bearing in mind that the use of di_erent excipients can tune the transdermal delivery of HQ significantly.Item In vitro effect of new formulations of amphotericin B on amastigote and promastigote forms of Leishmania infantum(International journal of antimicrobial agents, 2007) Ordóñez Gutiérrez, Lara; Espada Fernández, Raquel; Dea Ayuela, María Auxiliadora; Torrado Durán, Juan José; Bolas Fernández, Francisco; Alunda Rodríguez, José MaríaThe in vitro antileishmanial activities of various new amphotericin B (AMB) formulations were investigated, including microspheres of hydrophilic albumin with three AMB aggregation forms (monomeric, dimeric and multiaggregate) and the polymers of polylactic-co-glycolic acid, Resomer RG502 and RG503 with the multiaggregate AMB form. This in vitro study was performed on the extracellular promastigote form and the intracellular amastigote form of a canine strain of Leishmania infantum (UCM 20) using the infected J774 murine macrophage-like cell line. Albumin-encapsulated forms did not show any toxicity for murine cells and had lower median effective concentration (EC50) values (ca. 0.003 microg/mL) for L. infantum amastigotes than free formulations (0.03 microg/mL). In addition, the aggregation state of AMB had a notable effect on the antileishmanial activity of the drug. Results obtained in vitro point towards interest in monomeric AMB encapsulated in microspheres in the chemotherapeutic control of leishmaniasis.Item Increased Efficacy of Oral Fixed-Dose Combination of Amphotericin B and AHCC® Natural Adjuvant against Aspergillosis(Pharmaceutics, 2019) Pérez-Cantero, Alba; Serrano López, Dolores Remedios; Navarro Rodríguez, Patricia; Schätzlein, Andreas G.; Uchegbu, Ijeoma F.; Torrado Durán, Juan José; Capilla, JavierInvasive pulmonary aspergillosis represents one of the most serious fungal infections among immunocompromised patients. In this study, we aimed to analyze the in vivo e_cacy of prophylactic oral amphotericin B (AMB) encapsulated in modified chitosan-nanoparticles (Nanomerics’ Molecular Envelope Technology (MET)) supplemented with a standardized extract of cultured Lentinula edodes mycelia (AHCC®) in a murine model of pulmonary aspergillosis. We determined fungal burden and survival of mice and additionally, we carried out a cytokine analysis in an attempt to understand the immunomodulation of the extract. Our results evidenced equivalent e_cacy between orally administered AMB-MET and the intravenous liposomal AMB marketed formulation. Addition of the AHCC® supplement significantly improved e_cacy in terms of burden reduction and survival increase of both oral and intravenous AMB therapies compared to the untreated control group. Moreover, a protective e_ect of the extract was observed in terms of weight loss. Regarding the cytokine profiles, the Th1 immune response was stimulated in treated animals when compared to the control group. This response was marked by an enhancement in the MCP-1, GM-CSF, VEGF, RANTES and IL-17 levels and a decrease in the IL-6, a biomarker related to the severity of the infection.- Project number: 291
Item Creación de nuevo recurso educativo virtual para estudiantes de grado en Farmacia.(2023) Torrado Durán, Susana; Álvarez Álvarez, Covadonga; Ares Lombán, Irma; Ballesteros Papantonakis, María de la Paloma; Fernández Gutierrez, Jesús Miguel; Franco Gil, María Elvira; Martínez Caballero, María Aranzazu; Martínez Caballero, Marta; Notivoli Díez, Pablo; Rodríguez Torrado, David; Rodríguez Torrado, Marta; Torrado Salmerón, Carlos Félix; Torrado Durán, Guillermo; Torrado Durán, Juan José; Torrado Durán, Santiago; Torre Iglesias, Paloma Marina de laCreación de nuevo recurso educativo virtual para estudiantes de grado en Farmacia. El presente proyecto es continuación de un proyecto concedido con financiación el año 2019 y en él se continuarán desarrollando nuevos recursos educativos virtuales como los que ya se han hecho este curso, y que han tenido excelente acogida por los alumnos, para facilitar la adquisición del conocimiento de los estudiantes de Farmacia especialmente de aquellos temas en los que la bibliografía disponible es escasa como en el caso de la Asignatura de Tecnología Farmacéutica III en los temas dedicados a la industria farmacéutica. Item Toxicology of Blister Agents: Is Melatonin a Potential Therapeutic Option?(Diseases, 2021) Romero Martínez, Manuel Alejandro; Ramos Alonso, Eva; López Muñoz, Francisco; Ríos, Cristóbal de los; Egea, Javier; Gil Martín, Emilio; Pita Pita, Rene; Torrado Durán, Juan José; Serrano López, Dolores Remedios; Juberias, AntonioBlister or vesicant chemical warfare agents (CWAs) have been widely used in different military conflicts, including World War I and the Iran-Iraq War. However, their mechanism of action is not fully understood. Sulfur and nitrogen mustard exert toxic effects not only through the alkylation of thiol-bearing macromolecules, such as DNA and proteins, but also produce free radicals that can develop direct toxic effects in target organs such as the eyes, skin, and respiratory system. The lack of effective treatments against vesicant CWAs-induced injury makes us consider, in this complex scenario, the use and development of melatonin-based therapeutic strategies. This multifunctional indoleamine could facilitate neutralization of the oxidative stress, modulate the inflammatory response, and prevent the DNA damage, as well as the long-term health consequences mediated by vesicant CWAs-induced epigenetic mechanisms. In this context, it would be essential to develop new galenic formulations for the use of orally and/or topically applied melatonin for the prophylaxis against vesicant CWAs, as well as the development of post-exposure treatments in the near future.- Project number: 170
Item Creación de un nuevo recurso educativo virtual para estudiantes de grado en Farmacia(2018) Torrado Durán, Susana; Torrado Durán, Santiago; Ballesteros Papantonakis, Paloma; Franco Gil, Elvira; Torrado Durán, Juan José; Álvarez Álvarez, Covadonga; Torre Iglesias, Paloma; Serrano López, Dolores Remedios; Ares Lombán, Irma; Martínez Caballero, María Aranzazu; Martínez Caballero, Marta; Andrés Yagüe, Ana María; Rodríguez Torrado, Marta; García Herrero, Victor; Torrado Salmerón, Carlos; López Sánchez, Alicia; Fernández Gutierrez, Jesús Miguel; Torrado Durán, Guillermo