Person: Domínguez Godínez, Carmen Olalla
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First Name
Carmen Olalla
Last Name
Domínguez Godínez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
Identifiers
3 results
Search Results
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Item Diquafosol Delivery from Silicone Hydrogel Contact Lenses: Improved Effect on Tear Secretion(Journal of Ocular Pharmacology and Therapeutics, 2018) Domínguez Godínez, Carmen Olalla; Carracedo Rodríguez, Juan Gonzalo; Pintor Just, Jesús JerónimoPurpose: The aim of this study was to evaluate the ability to uptake and to deliver diquafosol from commercial contact lenses (CLs) and its effect on tear secretion. Methods: For both in vitro and in vivo experiments, two commercial silicone hydrogel (Si-Hy) CLs (comfilcon A and balafilcon A) were used. The CLs were soaked overnight for 12 h in diquafosol solution and control CLs were soaked in saline solution (NaCl 0.9%). The CLs were introduced into a new well container with 1 mL of saline solution, and aliquots of 100 μL were extracted at different times during a period of 6 h to measure the diquafosol release. For in vivo experiments, nine male New Zealand white rabbits were used. CLs soaked in diquafosol were inserted in the eye and compared with control CLs and diquafosol topical instillation. Schirmer's tests were performed to evaluate tear secretion and diquafosol release at different times during the 6-h period. Results: For in vitro experiments, the largest amount of diquafosol was released during the first 24 h for both CL materials under study, without statistical differences between them (P < 0.05). The topical application showed the maximum release at 1 min after instillation, meanwhile the release from both CL materials was at 30 min of insertion. The effect on tear secretion was higher with CL delivery compared with topical instillation (P < 0.05), being 300 min for both CLs and 90 min for topical application. Conclusion: The use of CLs increases the residence time of diquafosol on the ocular surface with a concomitant enhancement in tear secretion during longer periods.Item In vitro and in vivo delivery of the secretagogue diadenosine tetraphosphate from conventional and silicone hydrogel soft contact lenses(Journal of Optometry, 2013) Domínguez Godínez, Carmen Olalla; Martín Gil, Alba; Carracedo Rodríguez, Juan Gonzalo; Guzmán Aránguez, Ana Isabel; González Meijome, Jose Manuel; Pintor Just, Jesús JerónimoPurpose To evaluate the possible use of soft contact lenses (CL) to improve the secretagogue role of diadenosine tetraphosphate (Ap4A) promoting tear secretion. Methods Two conventional hydrogel CL (Omafilcon A and Ocufilcon D) and two silicone hydrogel (SiH) CL (Comfilcon A and Balafilcon A) were used. Ap4A was loaded into the lenses by soaking in a 1 mM Ap4A solution during 12 h. In vitro experiments were performed by placing the lenses in multi-wells during 2 h containing 1 ml of ultrapure water. 100 μl aliquots were taken at time zero and every minute for the first 10 min, and then every 15 min. In vivo experiments were performed in New Zealand rabbits and both the dinucleotide release from SiH and tear secretion were measured by means of Schirmer strips and high-pressure liquid chromatography (HPLC) analysis. Results Ap4A in vitro release experiments in hydrogel CL presented a release time 50 (RT50) of 3.9 ± 0.2 min and 3.1 ± 0.1 min for the non-ionic and the ionic CL, respectively. SiH CL released also Ap4A with RT50 values of 5.1 ± 0.1 min for the non-ionic and 2.7 ± 0.1 min for the ionic CL. In vivo experiments with SiH CL showed RT50 values of 9.3 ± 0.2 min and 8.5 ± 0.2 min for the non-ionic and the ionic respectively. The non-ionic lens Ap4A release was able to induce tear secretion above baseline tear levels for almost 360 min. Conclusion The delivery of Ap4A is slower and the effect lasts longer with non-ionic lenses than ionic lenses.Item Effect of Melatonin and Its Analogs on Tear Secretion(The Journal of pharmacology and experimental therapeutics, 2019) Navarro Gil, Francisco Javier; Huete Toral, Fernando; Crooke Álvarez, Almudena; Domínguez Godínez, Carmen Olalla; Carracedo Rodríguez, Juan Gonzalo; Pintor Just, Jesús JerónimoMelatonin has been shown to enhance tear secretion associated with dinucleotide diadenosine tetraphosphate. This study investigated the isolated action of melatonin and its analogs, agomelatine, N-butanoyl-2-(2-methoxy-6H-isoindolo[2,1-a]indol-11-yl) ethanamine (IIK7), and 5-methoxycarbonylamino-N-cetyltryptamine (5-MCA-NAT) (10 µl at 100 µM), on tear secretion when applied topically in the rabbit cornea and its relationship with the melatonin MT1, MT2, and MT3/quinone reductase QR2 receptors. The results showed a significant increase in tear secretion, with a maximal effect at 60 minutes for the agonists (138.9% ± 6.5%, 128.9% ± 6.4%, and 120.0% ± 5.2%, respectively; P < 0.05; 100% control) but not for melatonin (101.6% ± 7.9%; P > 0.05). Agonist action was tested combined with the antagonists DH97 (MT2 selective), prazosin (MT3/QR2 inhibitor), and luzindole (nonselective MT membrane receptor) (10 µl at 100 µM). DH97 reversed the effect of agomelatine, IIK7, and 5-MCA-NAT up to 30.85% ± 7.6%,108% ± 7.2%, and 87.01% ± 7.6%, respectively (P < 0.05; 100% control). Luzindole antagonized agomelatine and 5-MCA-NAT up to 67.35% ± 7.6% and 92.12% ± 8%, respectively (P < 0.05). Prazosin only reversed 5-MCA-NAT action up to 84.2% ± 7.7% (P < 0.05). These results suggest different pathways for the agonists to act through MT membrane receptors. Therefore, agomelatine, IIK7, and 5-MCA-NAT act through MT membrane receptors as secretagogues of tear secretion, and these analogs could be considered excellent therapeutic candidates for dry eye treatment.