Person:
Gómez-Serranillos Cuadrado, María Pilar

First Name

María Pilar

Last Name

Gómez-Serranillos Cuadrado

URI

https://hdl.handle.net/20.500.14352/77544

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Farmacia

Department

Farmacología, Farmacognosia y Botánica

Area

Farmacología

Identifiers

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Now showing 1 - 10 of 30

Effect of cooking and germination on phenolic composition and biological properties of dark beans (Phaseolus vulgaris L.)
(Food Chemistry, 2013) López, Ana; El-Naggar, Tarek; Dueñas, Montserrat; Ortega, Teresa; Estrella, Isabel; Hernández, Teresa; Gómez-Serranillos Cuadrado, María Pilar; Palomino Ruiz-Poveda, Olga María; Carretero Accame, María Emilia
Legumes are the base´s diet in several countries. They hold a high nutritional value, but other properties related to human health are nowadays being studied. The aim of this work was to study the influence of processes (boiling or germination) on the phenolic composition of dark beans (Phaseolus vulgaris L. c.v. Tolosana) and their effect on their antioxidant, neuroprotective and anticancer ability. Phenolic composition of raw and processed dark beans was analysed by HPLC-PAD and HPLC–ESI/MS. The antioxidant activity was evaluated by ORAC. Astrocytes cultures (U-373) have been used to test their neuroprotective effect. Anticancer activities were evaluated on three different cell lines (renal adenocarcinoma (TK-10), breast adenocarcinoma (MCF-7) and melanoma (UACC-62)) by sulphorhodamine B method. Qualitative and quantitative differences in phenolic composition have been observed between raw and processed dark beans that influence the antioxidant activity, mainly for germinated samples which show a decrease of antioxidant capacity. Although every assayed extracts decreased reactive oxygen species release and exhibited cytotoxicity activities on cancer cell lines, raw beans proved to be the most active in neuroprotective and antitumoral effects; this sample is especially rich in phenolic compounds, mainly anthocyanins. This study further demonstrated that phenolic composition of dark beans is related with cooking process and so with their neuroprotective and anticancer activity; cooking of dark beans improves their digestion and absorption at intestinal level, while maintaining its protective ability on oxidative process at cellular level.
Photoprotective Strategies in Mediterranean High-Mountain Grasslands
(Diversity, 2021) Magaña Ugarte, Rosina; Gómez-Serranillos Cuadrado, María Pilar; Escudero, Adrián; Gavilán García, Rosario Gloria
Albeit the remarkably high Ultraviolet B loads, high temperatures, and drought stress substantiate the need for efficient photoprotective strategies in Mediterranean high-mountain plants, these remain understudied. Considering the sensitivity of photosystems to extreme conditions, we evaluated an environmental gradient’s weight on the photoprotection of five high-mountain specialists from Central Spain. Diurnal and seasonal variations in chlorophyll, chlorophyll fluorescence, carotenoids, and xanthophylls in consecutive and climatically contrasting years were taken to evaluate the effect of the impending climate coarsening at the photosystem level. Our results revealed significant differences among species in the xanthophyll cycle functioning, acting either as a continuous photoprotective strategy enhancing photochemistry-steadiness; or prompted only to counteract the cumulative effects of atypically adverse conditions. The lutein cycle’s involvement is inferred from the high lutein content found in all species and elevations, acting as a sustained photoprotective strategy. These findings added to high de-epoxidation state (DEPS) and minor seasonal changes in the chlorophyll a/b ratio, infer the xanthophyll and Lutein cycles are crucial for upkeeping the photosystems’ optimal functioning in these plants heightening their photoprotective capacity during periods of more unfavorable conditions. Nevertheless, an atypically dry growing season’s detrimental effect infers the feasible surpassing of stress-thresholds and the precariousness of the communities’ functional diversity under climate change.
Project number: 249
Flipped Learning y Web App de Plantas Medicinales Nuevas estrategias educativas aplicadas al ámbito de la enseñanza en la asignatura de Farmacognosia y Fitoterapia
(2017) Gómez-Serranillos Cuadrado, María Pilar; González Burgos, Elena María; Fernández Moriano, Carlos; Iglesias Peinado, Irene; Gavilán García, Rosario Gloria; Garcia Broncano, Rosario
Antioxidant and antidiabetic activity and phytoconstituents of lichen extracts with temperate and polar distribution
(2023) Torres-Benítez, Alfredo ; Ortega-Valencia, José Erick ; Jara-Pinuer, Nicolás; Sánchez Gómez-Serranillos, Marta; Vargas-Arana, Gabriel ; Gómez-Serranillos Cuadrado, María Pilar; Simirgiotis, Mario J.
The objective of this research was to characterize the chemical composition of ethanolic extracts of the lichen species Placopsis contortuplicata, Ochrolechia frigida, and Umbilicaria antarctica, their antioxidant activity, and enzymatic inhibition through in vitro and molecular docking analysis. In total phenol content, FRAP, ORAC, and DPPH assays, the extracts showed significant antioxidant activity, and in in vitro assays for the inhibition of pancreatic lipase, α-glucosidase, and α-amylase enzymes, together with in silico studies for the prediction of pharmacokinetic properties, toxicity risks, and intermolecular interactions of compounds, the extracts evidenced inhibitory potential. A total of 13 compounds were identified by UHPLC-ESI-QTOF-MS in P. contortuplicata, 18 compounds in O. frigida, and 12 compounds in U. antarctica. This study contributes to the knowledge of the pool of bioactive compounds present in lichens of temperate and polar distribution and biological characteristics that increase interest in the discovery of natural products that offer alternatives for treatment studies of diseases related to oxidative stress and metabolic syndrome.
Influence of processing in the phenolic composition and health-promoting properties of lentils (Lens culinaris L.)
(Journal of Food Processing and Preservation, 2017) López Pérez, Ana Esther; El-Naggar, Tarek; Dueñas Patón, Montserrat; Ortega Hernández-Agero, María Teresa; Estrella, Isabel; Hernández Fernández, María Teresa; Gómez-Serranillos Cuadrado, María Pilar; Palomino Ruiz-Poveda, Olga María; Carretero Accame, María Emilia
'Lentils (Lens culinaris L.) are a source of polyphenolic compounds with health-promoting properties, but cooking processes before consumption cause hydrolytic reactions, endogenous enzymes and biochemical metabolism activation which could interfere with their positive physiological effects. The aim of this study was to assess the influence of boiling and germination in the phenolic content and composition of marketed lentils by liquid chromatography analysis and thus, to evaluate its impact on the neuroprotective and anticancer properties through cell culture assays. The protective effect toward astrocyte culture when submitted to an oxidant injury remained after processing while a decrease in the antioxidant activity by oxygen radical absorbing capacity assay and DPPH methods was detected. Every sample was cytotoxic on the assayed cancer cell lines, the strongest antiproliferative activity being for germinated lentils on melanoma cell line. In conclusion, processing of lentils led to significant chemical modifications in the polyphenolic content without significant influence on its healthy properties’
Risk of dementia among antidepressant elderly users: A population-based cohort analysis in Spain
(Journal of Affective Disorders, 2024) Santandreu, Javier; Caballero, Francisco Félix; Gómez-Serranillos Cuadrado, María Pilar; González Burgos, Elena María
The use of antidepressants with anticholinergic effects has been associated with an increased risk of dementia. However, the results published are contradictory. The aim of the study is to compare the risk of developing dementia in elderly who were prescribed tricyclic antidepressants (TCA) versus those who were prescribed selective serotonin reuptake inhibitors (SSRIs) and other antidepressants (OA). A prospective population-based cohort study was performed using the Spanish Database for Pharmacoepidemiological Research in Primary Care (BIFAP) data (from 2005 to 2018). The cohort study included 62,928 patients age ≥ 60 without dementia and with antidepressant long-term monotherapy. Patients were divided into exposure antidepressant groups based on ATC system [TCA, SSRIs users and OAs users]. The risk of dementia was calculated by Cox regression models, providing hazard ratios (HR) and 95 % confidence intervals. The Kaplan-Meier model was used for survival analysis. Chi2 test was used as association test. The results showed SSRI users had higher dementia risk than TCA users (HR = 1.864; 95%CI = 1.624–2.140). Moreover, OA users had also significant risk of dementia (HR = 2.103; 95%CI = 1.818–2.431). Several limitations are the variation of the trend in the prescription of antidepressants, the small number of patients that use some antidepressants, the lack of information related to the dose, or socioeconomic characteristics, the use of antidepressant drugs for other indications, or the therapeutic compliance. Our findings showed that older users of SSRI and OA have more risk of developing dementia than TCA elderly users. However, additional studies would be needed.
Self-assembling, supramolecular chemistry and pharmacology of amphotericin B: Poly-aggregates, oligomers and monomers
(Journal of Controlled Release, 2021) Raquel Fernández-García; Juan C. Muñoz-García; Matthew Wallace; Laszlo Fabian; González Burgos, Elena María; Gómez-Serranillos Cuadrado, María Pilar; Raposo González, Rafaela; Bolas Fernández, Francisco; Ballesteros Papantonakis, María De La Paloma; Anne Marie Healy; Yaroslav Z. Khimyak; Serrano López, Dolores Remedios
Antifungal drugs such as amphotericin B (AmB) interact with lipids and phospholipids located on fungal cell membranes to disrupt them and create pores, leading to cell apoptosis and therefore efficacy. At the same time, the interaction can also take place with cell components from mammalian cells, leading to toxicity. AmB was selected as a model antifungal drug due to the complexity of its supramolecular chemical structure which can self-assemble in three different aggregation states in aqueous media: monomer, oligomer (also known as dimer) and poly-aggregate. The interplay between AmB self-assembly and its efficacy or toxicity against fungal or mammalian cells is not yet fully understood. To the best of our knowledge, this is the first report that investigates the role of excipients in the supramolecular chemistry of AmB and the impact on its biological activity and toxicity. The monomeric state was obtained by complexation with cyclodextrins resulting in the most toxic state, which was attributed to the greater production of highly reactive oxygen species upon disruption of mammalian cell membranes, a less specific mechanism of action compared to the binding to the ergosterol located in fungal cell membranes. The interaction between AmB and sodium deoxycholate resulted in the oligomeric and polyaggregated forms which bound more selectively to the ergosterol of fungal cell membranes. NMR combined with XRD studies elucidated the interaction between drug and excipient to achieve the AmB aggregation states, and ultimately, their diffusivity across membranes. A linear correlation between particle size and the efficacy/toxicity ratio was established allowing to modulate the biological effect of the drug and hence, to improve pharmacological regimens. However, particle size is not the only factor modulating the biological response but also the equilibrium of each state which dictates the fraction of free monomeric form available. Tuning the aggregation state of AmB formulations is a promising strategy to trigger a more selective response against fungal cells and to reduce the toxicity in mammalian cells.
DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS)
(Plants, 2020) Sánchez Gómez-Serranillos, Marta; González Burgos, Elena María; Dulare Devi, Pradeep Divakar; Gómez-Serranillos Cuadrado, María Pilar
There is growing interest for medicinal plants in the world drug market. Particularly, Matricaria recutita L., Valeriana officinalis L., Tilia spp., and Camellia sinensis (L.) Kuntze are some of the most consumed medicinal plants for treatment of minor health problems. Medicinal plants are seen as natural and safe; however, they can cause interactions and produce adverse reactions. Moreover, there is lack of consensus in medicinal plants regulation worldwide. DNA barcoding and UHPLC-MS technique are increasingly used to correctly identify medicinal plants and guarantee their quality and therapeutic safety. We analyzed 33 samples of valerian, linden, tea, and chamomile acquired in pharmacies, supermarkets, and herbal shops by DNA barcoding and UHPLC-MS. DNA barcoding, using matk as a barcode marker, revealed that CH1 sold as Camellia sinensis was Blepharocalyx tweediei, and sample TS2 sold as linden belong to Malvales. On the other hand, UHPLC-MS analysis revealed the presence of bioactive compounds (apigenin-7-glucoside, acetoxy valerenic acid, valerenic acid, epigallocatechin, and tiliroside). However, none of samples met minimum content of these active principles (except for valerenic acid in VF3) according to the European Medicines Agency (EMA) and Real Spanish Pharmacopeia. In conclusion, this study revealed the need to incorporate DNA barcoding and HPLC-MS techniques in quality controls of medicinal plants.
UHPLC-MS Chemical Fingerprinting and Antioxidant, Enzyme Inhibition, Anti-Inflammatory In Silico and Cytoprotective Activities of Cladonia chlorophaea and C. gracilis (Cladoniaceae) from Antarctica
(2022) Torres-Benítez, Alfredo ; Ortega-Valencia, José Erick ; Sánchez Gómez-Serranillos, Marta; Hillmann-Eggers, Mathias ; Gómez-Serranillos Cuadrado, María Pilar; Vargas-Arana, Gabriel ; Simirgiotis, Mario J.
The lichen species Cladonia chlorophaea and C. gracilis (Cladoniaceae) are widely distributed in the island archipelago of maritime Antarctica and represent a natural resource of scientific interest. In this work, the metabolomic characterization of the ethanolic extracts of these species and the determination of the antioxidant activity, enzymatic inhibition and anti-inflammatory potential of selected compounds on the 5-lipoxygenase enzyme by molecular docking and cytoprotective activity in the SH-SY5Y cell line were carried out. Nineteen compounds were identified by liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS) in each of the species. The contents of phenolic compounds, antioxidant activity, the inhibition of cholinesterases (acetylcholinesterase and butyrylcholinesterase) and digestive enzymes (α-glucosidase and pancreatic lipase) were variable among species, with better results in C. chlorophaea. Molecular docking evidenced significant binding affinities of some compounds for the 5-lipoxygenase enzyme, together with outstanding pharmacokinetic properties. Both extracts were shown to promote cell viability and a reduction in reactive oxygen species production in an H2O2-induced oxidative stress model. This study contributes to the chemical knowledge of the Cladonia species and demonstrates the biological potential for the prevention and promising treatment of central nervous system pathologies, inflammatory disorders and metabolic alterations.
Current knowledge on Parmelia genus: Ecological interest, phytochemistry, biological activities and therapeutic potential
(2019) González Burgos, Elena María; Fernández Moriano, Carlos; Gómez-Serranillos Cuadrado, María Pilar
Parmelia Acharius is one of the most representative genera within Parmeliaceae family which is the largest and the most widespread family of lichen-forming fungi. Parmelia lichens present a medium to large foliose thallus and they are distributed from the Artic to the Antartic continents, being more concentrated in temperate regions. According to its current description, the genus encompasses up to 41 different species and it is phylogenetically located within the Parmelioid clade (the largest group in the family). Interestingly, some of its species are among the most common epiphytic lichens in Europe such as Parmelia sulcata Taylor and Parmelia saxatilis (L.) Ach. The present work aims at providing a complete overview of the existing knowledge on the genus, from general concepts such as taxonomy and phylogeny, to their ecological relevance and biological interest for pharmaceutical uses. As reported, Parmelia lichens arise as valuable tools for biomonitoring environmental pollution due to their capacity to bioaccumulate metal elements and its response to acid rain. Moreover, they produce a wide array of specialized products/metabolites including depsides, depsidones, triterpenes and dibenzofurans, which have been suggested to exert promising pharmacological activities, mainly antimicrobial, antioxidant and cytotoxic activities. Herein, we discuss past and recent data regarding to the phytochemical characterization of more than 15 species. Even though the knowledge is still scarce in comparsion to other groups of organisms such as higher plants and other non-lichenized fungi. Reviewed works suggest that Parmelia lichens are worthy of further research for determining their actual possibilities as sources of bioactive compounds with potential therapeutic applications.