Person: López Cabarcos, Enrique
Universidad Complutense de Madrid
Faculty / Institute
Química en Ciencias Farmacéuticas
Now showing 1 - 10 of 14
PublicationOligonucleotide Sensor Based on Selective Capture of Upconversion Nanoparticles Triggered by Target Induced DNA Inter-Strand Ligand Reaction(Amer Chemical Soc., 2017-03-23) Méndez González, Diego; Laurenti, Marco; Latorre, Alfonso; Somoza, Álvaro; Vázquez, Ana; Negredo, Ana Isabel; López Cabarcos, Enrique; Calderón, Oscar Gómez; Melle Hernández, Sonia; Rubio Retama, JorgeWe present a sensor that exploits the phenomenon of upconversion luminescence to detect the presence of specific sequences of small oligonucleotides like miRNAs among others. The sensor is based on NaYF4:Yb,Er@SiO2 nanoparticles functionalized with ssDNA that contain azide groups on the 3' ends. In the presence of a target sequence, inter-strand ligation is possible via click-reaction between one azide of the upconversion probe and a DBCO-ssDNA-biotin probe present in the solution. As result of this specific and selective process, biotin is covalently attached to the surface of the upconversion nanoparticles. The presence of biotin on the surface of the nanoparticles allows their selective capture on a streptavidin-coated support, giving a luminescent signal proportional to the amount of target present in the test samples. With the aim of studying the analytical properties of the sensor, total RNA samples were extracted from healthy mosquitoes and spiked-in with a specific target sequence at different concentrations. The result of these experiments revealed that the sensor was able to detect 10-17 moles (100 fM) of the target sequence in mixtures containing 100 ng of total RNA per well. Similar limit of detection was found for spiked human serum samples, demonstrating its suitability for detecting specific sequences of small oligonucleotides under real conditions. By contrast, under the presence of non-complementary sequences or sequences having mismatches, the luminescent signal was negligible or conspicuously reduced. PublicationControl of upconversion luminescence by gold nanoparticle size: from quenching to enhancement(RSC, 2019-08-07) Méndez González, Diego; Melle Hernández, Sonia; Gómez Calderón, Óscar; Laurenti, Marco; Cabrera Granado, Eduardo; Egatz-Gómez, Ana; López Cabarcos, Enrique; Rubio Retama, Jorge; Díaz García, ElenaMetallic nanostructures have the potential to modify the anti-Stokes emission of upconverting nanoparticles (UCNPs) by coupling their plasmon resonance with either the excitation or the emission wavelength of the UCNPs. In this regard gold nanoparticles (AuNPs) have often been used in sensors for UCNP luminescence quenching or enhancement, although systematic studies are still needed in order to design optimal UCNP–AuNP based biosensors. Amidst mixed experimental evidence of quenching or enhancement, two key factors arise: the nanoparticle distance and nanoparticle size. In this work, we synthesize AuNPs of different sizes to assess their influence on the luminescence of UCNPs. We find that strong luminescence quenching due to resonance energy transfer is preferentially achieved for small AuNPs, peaking at an optimal size. A further increase in the AuNP size is accompanied by a reduction of luminescence quenching due to an incipient plasmonic enhancement effect. This enhancement counterbalances the luminescence quenching effect at the biggest tested AuNP size. The experimental findings are theoretically validated by studying the decay rate of the UCNP emitters near a gold nanoparticle using both a classical phenomenological model and the finite-difference time-domain method. Results from this study establish general guidelines to consider when designing sensors based on UCNPs–AuNPs as donor–quencher pairs, and suggest the potential of plasmon-induced luminescence enhancement as a sensing strategy. PublicationUltrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging(Nature Publishing Group, 2020-06-10) Santos, Harrison D. A.; Zabala Gutiérrez, Irene; Shen, Yingli; Lifante, José; Ximendes, Erving; Laurenti, Marco; Méndez González, Diego; Melle Hernández, Sonia; Gómez Calderón, Óscar; López Cabarcos, Enrique; Fernández Monsalve, Nuria; Chavez Coria, Irene; Lucena Agell, Daniel; Monge, Luis; Mackenzie, Mark D.; Marqués Hueso, José; Jones, Callum M. S.; Jacinto, Carlos; Rosal, Blanca, del; Kar, Ajoy K.; Rubio Retama, Jorge; Jaque García, DanielOptical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm−2) and doses (<0.5 mg kg−1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing. PublicationLas TICs: un instrumento de ayuda en las prácticas de laboratorio(2022-01-31) Laurenti, Marco; Ovejero Paredes, Karina; Zabala Gutiérrez, Irene; Sanchez Sanche, Visitación; Yusta San Roman, Piedad; Contreras Cáceres, Rafael; Filice, Marco; López Cabarcos, Enrique; Marciello, Marzia; Méndez González, Diego; Rubio Retama, Benito Jorge; Villaverde Cantizano, GonzaloDesde la implantación del Espacio Europeo de Educación Superior nos encontramos ante dos grandes retos; el desarrollo metodológico y tecnológico en la formación y docencia on-line y en segundo lugar la cada vez más elevada especialización requerida a los estudiantes durante el Grado y Doble Grado en Farmacia. Esto hace que el foco de la formación de los estudiantes se desvíe de los conocimientos básicos y competencias de formación tanto en el trabajo experimental como en el conocimiento teórico. El proyecto se encuadra en el marco de la asignatura obligatoria Física Aplicada a Farmacia de Grado y Doble Grado en Farmacia, unas carreras con una elevada carga práctica y experimental. PublicationEffect of platelet-rich plasma on sinus lifting: a randomized-controlled clinical trial(Wiley-Blackwell, 2009) Torres García-Denche, Jesús; Tamimi, Faleh; Martinez, Pedro Pablo; Alkhraisat, Mohammad Hamdan; Linares, Rafael; Hernández, Gonzalo; Torres Macho, Juan; López Cabarcos, EnriqueObjective: The combination of anorganic bovine bone (ABB) with platelet-rich plasma (PRP)has been widely used in bone regeneration procedures although benefits are still unclear. The purpose of this study was to evaluate whether or not PRP improves the efficacy of ABB in sinus floor augmentation. In addition, we have investigated the effect of residual bone height and tobacco on implant survival in sinus augmentation procedures. Patient and methods: Eighty-seven patients recruited for this study under went 144 sinus floor augmentation procedures using ABB alone or ABB plus PRP (ABB +PRP) in a randomized clinical trial. A total of 286 implants were placed in the augmented bone, and their evolution was followed up for a period of 24 months. In order to investigate on a histological level and any adjunctive effects, we performed an ancillary study in five edentulous patients with a symmetrical severely resorbed maxilla. In these patients, a bilateral sinus augmentation was randomly performed using ABB or ABB+PRP in a splt-mouth design,and after 6 months, bone biopsies were taken from the implant sites for histological and histomorphometric analysis. Results: Overall, 96.2% of ABB and 98.6% of ABB+PRP implant sucess were obtained during the monitoring period and differences were not found between sites grafted with and without PRP in the 87 patients studied. Densitometry assessments and graft resorption were similar in both experimental groups. However, the histological and histomorphometrical analysis in the five edentulous patients revealed that bone augmentation was significantly higher in sites treated with ABB+PRP (p< o igual que 0.05). Another outcome from our study is that the lack of initial bone support (p< o igual que 0.05) and smoking (p=0.05) appeared to have a negative effect on the treatment success, which was accentuated when both circumstances coincided. Conclusions: PRP is not a determined factor for implant survival in sinus lifting procedures. However, this study revealed that PRP can improve the osteoconductive properties of ABB by increasing the volume of new bone formed. Moreover, in sinus augmentation procedures the implant's survival rate appears to be more influenced by the residual bone height or by tobacco than by the type of bone graft. PublicationBone augmentation in rabbit calvariae: comparative study between Bio-Oss® and a novel B-TCP/DCPD granulate(Wiley-Blackwell, 2006) Tamimi, Faleh; Torres García-Denche, Jesús; Tresguerres, Isabel; Clemente, Celia; López Cabarcos, Enrique; Blanco Jerez, LuisAim: In the present in vivo study, we compare the bone regeneration capacity of a novel brushite cement synthesized in our laboratory (DTG) with Bio-Oss® using rabbits as an animal model. Methods: The study was performed in a group of 14 adult New Zealand rabbits using the bone conduction model. Two titanium cylinders were fixed into perforated slits made on the parietal cortical bone of each rabbit. One cylinder was left empty (negative control) and the other was filled with either Bio-Oss® or brushite set cement granules (test cylinder). Four weeks after the intervention, the animals were sacrified and biopsies were taken. The following parameters were analysed: bone tissue augmentation, bone mineral density and biomaterials resorption. The comparison of data between the different groups was performed using the Mann-Whitney test with a significance level of p<0.05. Results: The mean bone mineral density and augmented mineral tissue inside the test cylinders were similar but higher than those of negative controls. Material resorption and bone tissue augmentation were significantly higher in the defects treated with the brushite-based set cement (p<0.05). Conclusions: Brushite cement granules were more resorbable and generated more bone tissue than Bio-Oss® inside the titanium cylinders placed in the rabbit calvaria. PublicationStrontium Ions Substitution in Brushite Crystals: The Role of Strontium Chloride(MDPI, 2011-05-31) Alkhraisat, Mohammad H.; Rueda, Carmen; López Cabarcos, EnriqueThe incorporation of strontium chloride to brushite cement was successful to introduce strontium ions within the lattice of brushite crystals. The effect of strontium ions on brushite cement properties was concentration dependent; such that, the addition of 5% and 10% (w/w) SrCl2 significantly increased the cement FST and the addition of 10% SrCl2 decreased the cement tensile strength. Further, cement weight loss was shown to be increased by cement modification with SrCl2. The combination of ionic substitution and the degradability of brushite cements would constitute a system for the local delivery of strontium ions in the treatment of osteoporosis. PublicationMatrix tablets based on a novel poly (magnesium acrylate) hydrogel for the treatment of inflammatory bowel diseases(Elsevier, 2021-09-27) Simancas Herbada, Rebeca; Torres Suárez, Ana Isabel; Otero Espinar, Francisco; Fraguas Sánchez, Ana Isabel; López Cabarcos, Enrique; Rubio Retama, Jorge; Fernández Carballido, Ana MaríaThe objective of this work was to evaluate the potential use of a new polymer (PAMgA) in the development sustained release matrix tablets for the treatment of bowel inflammatory diseases. For this purpose, budesonide, a highly lipophilic compound, was used as model drug. Tablets with two reticulation grades of PAMgA (PAMgA 5 and 40) and with 9 mg of budesonide were developed and characterized. All the studies were carried out using biorelevant media (FaSSGF and FaSSIF). Swelling and erosion of PAMgA tablets was influenced by the reticulation grade of the polymer and the biorelevant media assayed, being water uptake higher for PAMgA 40 tablets in intestinal fluid, whereas PAMgA 5 showed more intense erosion in this biorelevant medium. Budesonide was released slowly from PAMgA tablets, both in gastric and intestinal environment, following Super case II transport kinetics (relaxation-controlled delivery), with a lag time of around 1–2 h. When the dissolution medium was changed sequentially throughout the trial, 75% of the budesonide dose was released in a sustained manner between 4 and 20 h of testing from PAMgA tablets, showing a more controlled budesonide release than Entocort® and Budenofalk® (commercially available sustained release formulations of budesonide). In conclusion, PAMgA polymer allows controlling the release of highly lipophilic drugs as budesonide, being an useful excipient for the development of sustained release matrix tablets. PublicationPlatelet-rich plasma may prevent titanium-mesh exposure in alveolar ridge augmentation with anorganic bovine bone(Wiley-Blackwell, 2010) Torres García-Denche, Jesús; Tamimi, Faleh; Alkhraisat, Mohammad Hamdan; Manchon, Angel; Linares, Rafael; Prados Frutos, J. C.; Hernández, Gonzalo; López Cabarcos, EnriqueObjective: Bone augmentation with the titanium-mesh (Ti-mesh) technique is susceptible to a large rate of complications such as morbidity of bone graft donor site, and mesh exposure to the oral cavity. The purpose of this study was to evaluate the effectiveness of anorganic bovine bone (ABB) in alveolar bone augmentation with the Ti-mesh technique. In addition, we investigated the effect of platelet-rich plasma (PRP) in preventing mesh exposure by using it to cover the Ti-mesh. Patients and methods: Patients included in the clinical trial were randomly allocated by a blinded assistant into two groups. The 30 patients recruited for this study underwent 43 alveolar bone augmentation with the Ti-mesh technique using ABB as graft material in all of them. In 15 patients, the Ti-meshes were covered with PRP (PRP group) whereas in the other 15 the Ti-meshes were not (control group). After 6 months, patients were called for clinical, radiographic and histological evaluation, and implant placement surgery. A total of 97 implants were placed in the augmented bone and their evolution was followed up for a period of 24 months. Results: Significant differences were found between the two study groups in terms of complications and bone formation. In the control group, 28,5% of the cases suffered from mesh exposure, while in the PRP group, no exposures were registered. Radiographic analysis revealed that bone augmentation was higher in the PRP group than in the control group. Overall, 97,3% of implants placed in the control group and 100% of those placed in the PRP group were successful during the monitoring period. We suggest that the positive effect of PRP on the Ti-mesh technique is due to its capacity to improve soft tissue healing, thereby protecting the mesh and graft material secured beneath the gingival tissues. Conclusions: Alveolar bone augmentation using ABB alone in the Ti-Mesh technique is sufficient for implant rehabilitation. Besides, covering the Ti-meshes with PRP was a determining factor in avoiding mesh exposure. Ti-Mesh exposure provoked significant bone loss, but in most cases it did not affect the subsequent placement implants. PublicationFRET distance dependence from upconverting nanoparticles to quantum dots(Amer Chemical Soc, 2018-05) Melle Hernández, Sonia; Gómez Calderón, Óscar; Laurenti, Marco; Méndez González, Diego; Egatz-Gómez, Ana; López Cabarcos, Enrique; Cabrera Granado, Eduardo; Díaz García, Elena; Rubio Retama, JorgeFörster resonant energy transfer (FRET) with upconverting nanoparticles (UCNPs) as donors and quantum dots (QDs) as acceptors has been regarded as a promising tool for biosensing applications. In this work, we use time-resolved fluorescence spectroscopy to analyze the UCNP-to-QD FRET and we focus on the most relevant parameter of the FRET phenomenon, UCNP-QD distance. This distance is controlled by a nanometric silica shell around the UCNP surface. We theoretically reproduce the experimental results applying FRET theory to the distribution of emitting erbium ions in the UCNP. This simple model allows us to estimate the contribution of every erbium ion to the final FRET response and to explore different strategies to improve FRET efficiency.