Person: Rodríguez Crespo, José Ignacio
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First Name
José Ignacio
Last Name
Rodríguez Crespo
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
Identifiers
9 results
Search Results
Now showing 1 - 9 of 9
- Project number: 8
Item ¿Qué es lo que sabemos… sobre Biología?(2021) Batanero Cremades, Eva; Rodríguez Crespo, José Ignacio; Castillo Lluva, Sonia; García Álvarez, Begoña; Bueno Díaz, CristinaDada la importancia del inglés y de las tecnologías móviles hoy día, los estudiantes utilizarán dichas tecnologías para reforzar el aprendizaje de Biología, y motivarlos a estudiar, mediante dos actividades: crear test Kahoot! en inglés y concursar. Item Nitric Oxide Down-regulates Caveolin-3 Levels through the Interaction with Myogenin, Its Transcription Factor(Journal of biological chemistry, 2007) Martínez-Moreno, Mónica; Martínez Ruiz, Antonio; Álvarez-Barrientos, Alberto; Gavilanes Franco, Francisco; Lamas, Santiago; Rodríguez Crespo, José IgnacioCertain patients suffering from chronic diseases such as AIDS or cancer experience a constant cellular secretion of tumor necrosis factor and other pro-inflammatory cytokines that results in a continuous release of nitric oxide ( NO) to the bloodstream. One immediate consequence of the deleterious action of NO is weight loss and the progressive destruction of muscular mass in a process known as cachexia. We have previously reported that caveolin-3, a specific marker of muscle cells, becomes down-regulated by the action of NO on muscular myotubes. We describe herein that the changes observed in caveolin-3 levels are due to the alteration of the DNA binding activity of the muscular transcription factor myogenin. In the presence of NO, the binding of transcription factors from cell nuclear extracts of muscular tissues to the E boxes present in the caveolin-3 promoter become substantially reduced.When we purified recombinant myogenin and treated it with NO donors, we could detect its S-nitrosylation by three independent methods, suggesting that very likely one of the cysteine residues of the molecule is being modified. Given the role of myogenin as a regulatory protein that determines the level of multiple muscle genes expressed during late myogenesis, our results might represent a novel mode of regulation of muscle development under conditions of nitric oxide-mediated toxicity.- Project number: 194
Item “¿Qué es lo que sabemos… sobre Biología”(2020) Batanero Cremades, Eva; Rodríguez Crespo, José Ignacio; San Segundo Acosta, Pablo; Bueno Díaz, Cristina; Parrón Ballesteros, JorgeDada la importancia del inglés y de las tecnologías móviles hoy día, los estudiantes utilizarán dichas tecnologías para reforzar el aprendizaje de Biología, y motivarlos a estudiar, mediante dos actividades: crear test Kahoot en inglés y concursar. - Project number: 326
Item Hacia una Universidad Complutense más diversa: actividades de visibilización del colectivo LGTBI en las Facultades de Químicas y Biológicas(2022) Rodríguez Crespo, José Ignacio; García Ortega, Lucía; Guzmán Pastor, Manuel; Narbona Corral, Javier; Gutiérrez Carmona, Adrián; Arauco Arteche, Iñigo; Ballesteros Sanabria, Laura; Castromil Benito, Estela Soraya; Amigot Sánchez, Rafael; Cueto Remacha, Mateo; Martín Migallón, Guillermo Item S-nitrosylation of Hsp90 promotes the inhibition of its ATPase and endothelial nitric oxide synthase regulatory activities(Proceedings of the National Academy of Science of the United States os America, 2005) Martínez Ruiz, Antonio; Villanueva, Laura; González de Orduña, Cecilia; López-Ferrer, Daniel; Higueras, María Ángeles; Tarín, Carlos; Rodríguez Crespo, José Ignacio; Vázquez, Jesús; Lamas, Santiago; Ignarro, Louis J.Nitric oxide is implicated in a variety of signaling pathways in different systems, notably in endothelial cells. Some of its effects can be exerted through covalent modifications of proteins and, among these modifications, increasing attention is being paid to S-nitrosylation as a signaling mechanism. In this work, we show by a variety of methods (ozone chemiluminescence, biotin switch, and mass spectrometry) that the molecular chaperone Hsp90 is a target of S-nitrosylation and identify a susceptible cysteine residue in the region of the C-terminal domain that interacts with endothelial nitric oxide synthase (eNOS). We also show that the modification occurs in endothelial cells when they are treated with S-nitrosoL-cysteine and when they are exposed to eNOS activators. Hsp90 ATPase activity and its positive effect on eNOS activity are both inhibited by S-nitrosylation. Together, these data suggest that S-nitrosylation may functionally regulate the general activities of Hsp90 and provide a feedback mechanism for limiting eNOS activation.Item Selective inhibition of cannabinoid CB1 receptor-evoked signalling by the interacting protein GAP43(Neuropharmacology, 2023) Maroto Martínez, Irene Berenice; Moreno, Estefanía; Costas Insúa, Carlos; Merino Gracia, Javier; Diez-Alarcia, Rebeca; Álvaro-Blázquez, Alicia; Canales Mayordomo, María Ángeles; Canela, Enric I.; Casadó, Vicent; Urigüen, Leyre; Rodríguez Crespo, José Ignacio; Guzmán Pastor, ManuelCannabinoids exert pleiotropic effects on the brain by engaging the cannabinoid CB1 receptor (CB1R), a presynaptic metabotropic receptor that regulates key neuronal functions in a highly context-dependent manner. We have previously shown that CB1R interacts with growth-associated protein of 43 kDa (GAP43) and that this interaction inhibits CB1R function on hippocampal excitatory synaptic transmission, thereby impairing the therapeutic effect of cannabinoids on epileptic seizures in vivo. However, the underlying molecular features of this interaction remain unexplored. Here, we conducted mechanistic experiments on HEK293T cells co-expressing CB1R and GAP43 and show that GAP43 modulates CB1R signalling in a strikingly selective manner. Specifically, GAP43 did not affect the archetypical agonist-evoked (i) CB1R/Gi/o protein-coupled signalling pathways, such as cAMP/PKA and ERK, or (ii) CB1R internalization and intracellular trafficking. In contrast, GAP43 blocked an alternative agonist-evoked CB1R-mediated activation of the cytoskeleton-associated ROCK signalling pathway, which relied on the GAP43-mediated impairment of CB1R/Gq/11 protein coupling. GAP43 also abrogated CB1R-mediated ROCK activation in mouse hippocampal neurons, and this process led in turn to a blockade of cannabinoid-evoked neurite collapse. An NMR-based characterization of the CB1R-GAP43 interaction supported that GAP43 binds directly and specifically through multiple amino acid stretches to the C-terminal domain of the receptor. Taken together, our findings unveil a CB1R-Gq/11-ROCK signalling axis that is selectively impaired by GAP43 and may ultimately control neurite outgrowth.- Project number: 434
Item Hacia una universidad complutense más diversa: actividades de visibilización del colectivo lgtbi en las facultades de químicas y biológicas (continuación)(2023) Rodríguez Crespo, José Ignacio; Guzmán Pastor, Manuel; García Ortega, Lucía; Fernández Fernández, Manuel; Amigot Sánchez, Rafael; Cueto Remacha, Mateo; Martín Migallón, Guillermo Item Molecular dissection of the membrane aggregation mechanisms induced by monomeric annexin A2(Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2018) López Rodríguez, Juan Carlos; Martínez-Carmona, Francisco J.; Rodríguez Crespo, José Ignacio; Lizarbe Iracheta, María Antonia; Turnay Abad, Francisco JavierAnnexins are a multigene family of proteins involved in aggregation and fusion processes of biological membranes. One of its best-known members is annexin A2 (or p36), capable of binding to acidic phospholipids in a calcium-dependent manner, as occurs with other members of the same family. In its heterotetrameric form, especially with protein S100A10 (p11), annexin A2 has been involved as a determinant factor in innumerable biological processes like tumor development or anticoagulation. However, the subcellular coexistence of different pools of the protein, in which the monomeric form of annexin A2 is growing in functional relevance, is to date poorly described. In this work we present an exhaustive structural and functional characterization of monomeric human annexin A2 by using different recombinant mutants. The important role of the amphipathic N-terminal α-helix in membrane binding and aggregation has been analyzed. We have also studied the potential implication of lateral "antiparallel" protein dimers in membrane aggregation. In contrast to what was previously suggested, formation of these dimers negatively regulate aggregation. We have also confirmed the essential role of three lysine residues located in the convex surface of the molecule in calcium-free and calcium-dependent membrane binding and aggregation. Finally, we propose models for annexin A2-mediated vesicle aggregation mechanisms.Item Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation(Journal of Materials Chemistry B, 2020) Illescas Martínez, Beatriz María; Pérez Sánchez, Alfonso; Mallo, Araceli; Martín Doménech, Ángel; Rodríguez Crespo, José Ignacio; Martín León, NazarioNon-viral nucleic acid vectors able to display high transfection efficiencies with low toxicity and overcoming the multiple biological barriers are needed to further develop the clinical applications of gene therapy. The synthesis of hexakis-adducts of [60]fullerene endowed with 12, 24 and 36 positive ammonium groups and a tridecafullerene appended with 120 positive charges has been performed. The delivery of a plasmid containing the green fluorescent protein (EGFP) gene into HEK293 (Human Embryonic Kidney) cells resulting in effective gene expression has demonstrated the efficacy of these compounds to form polyplexes with DNA. Particularly, giant tridecafullerene macromolecules have shown higher efficiency in the complexation and transfection of DNA. Thus, they can be considered as promising non-viral vectors for transfection purposes.