Person:
Fuertes Recuero, Manuel

First Name

Manuel

Last Name

Fuertes Recuero

URI

https://hdl.handle.net/20.500.14352/80506

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Veterinaria

Department

Fisiología

Area

Zoología

Identifiers

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Now showing 1 - 10 of 15

First report of polymelia in a passerine bird, the Pied Flycatcher
(Ardea, 2023) Fontanillas Pérez, Juan Carlos; Cantarero Carmona, Alejandro; Fuertes Recuero, Manuel; Longhin, Davide; Chiesurin, Leonardo; Tusini, Samuele
Polymelia is a congenital defect characterized by an excessive number of limbs. Leg malformations are unusual and have rarely been reported in birds. The ultimate mechanism that regulates this type of abnormal development is not yet well understood. Here we report polymelia in a nestling of the European Pied Flycatcher Ficedula hypoleuca. We have ringed more than 12,100 Pied Flycatcher nestlings since 1991 and have never observed this malformation before. To our knowledge, there is no study indicating polymelia in any bird of the order Passeriformes. We observed and studied a Pied Flycatcher nestling that had two extra limbs fused at the pelvis. We used X-rays, Computerized Tomography (CT) scan and Magnetic Resonance Imaging (MRI). We describe the observed malformations and discuss potential causes. The malformation could be due to an embryonic cause, such as a parasitic twin (pygopagus parasitic) or a genetic mutation.
Description of postoperative complications and bacterial contamination of wound soaker catheters used to administer postoperative local analgesia after mastectomy in 11 dogs: case series
(Veterinary Research Communications, 2024) Suárez Redondo, María; Fuertes Recuero, Manuel; Guzmán Soltero, Alba; Aguado Domínguez, Delia; Martín Espada, María Del Carmen; Espinel Rupérez, Jorge; Ortiz Díez, Gustavo
Mastectomy is a common and painful procedure in dogs. Wound soaker catheters (WSC) are frequently used to reduce postoperative pain, including pain after mastectomy. The objectives of this case series were to describe the use of WSC for owner administration of postoperative local analgesia in dogs with mammary tumors treated surgically, to identify complications associated with WSC and to determine the frequency of bacterial colonization of the catheters. Twelve WSC were placed in 11 dogs during mastectomy surgery, left in place for three days, protected by a dressing and successfully managed by owners at home. No postoperative antibiotics were administered. No complications were identified in any cases. No bacterial growth was identified on bacteriological analysis of the twelve WSC. These results suggest that the use of WSC is a safe alternative for postoperative analgesia administration following mastectomy in dogs. Future studies comparing dogs with or without WSC with a larger number of dogs are needed to further evaluate efficacy and complications.
Project number: 199
Desarrollo de material audiovisual (zootomías de diversas especies animales) aplicable a prácticas del campo de la Zoología, Anatomía y Biología, para su utilización en un entorno virtual
() Fontanillas Pérez, Juan Carlos; Álvarez López, Alberto; Fuertes Recuero, Manuel; García-Cuenca Ariati, Isabel; Cantarero Carmona, Alejandro; González Gil, Alfredo; Jerónimo Sánchez, Benedicto; Martínez Sainz, María Del Pilar; Picazo González, Rosa Ana; Ramírez Gómez, Esther
La ejecución del proyecto ha permitido la obtención del material audiovisual previsto en los objetivos iniciales. Este material comprende 8 videos correspondientes a las X prácticas incluidas en el objetivo inicial del proyecto, en los que se han incrustado las imágenes, vídeos y locución en cada uno de ellos. Se ha intentado generar un material atractivo para el alumno, en el que se le explica con voz en off o en la propia diapositiva los distintos apartados para que se pueda comprender fácilmente la práctica. Los videos se colgarán en el Campus Virtual de las asignaturas el próximo curso, antes de las sesiones prácticas, en la carpeta correspondiente de prácticas. Se dejará a disposición de los estudiantes todo el curso. Será una importante herramienta de apoyo para los docentes, ya que el estudiante deberá de previsualizarlas antes de ir a la sesión de prácticas correspondiente. Por tanto, servirá claramente para el objetivo que se planteó. Cada video constituye un material e-learning, puesto que el estudiante puede visualizarlo tantas veces como desee y en cualquier momento, lo que favorece el aprendizaje autónomo. Por otro lado, el profesor puede hacer una aproximación a la metodología de flipped learning en la que el estudiante comprende y prepara los contenidos antes de ir a la sesión práctica. Con el material generado en este proyecto los estudiantes realizarán los procedimientos experimentales de manera más rápida y eficaz, ya que han visualizado previamente cómo se realizaban, o incluso podrán ir siguiendo los pasos en la sesión, de forma autónoma, consultando el contenido online disponible. La previa del contenido online puede ayudar a desarrollar sesiones prácticas en las que también se puedan resolver dudas y cuestiones complejas, al tener más tiempo.
Do Barn Swallow nestlings incur an oxidative cost of begging?
(Journal of Ornithology, 2023) Fuertes Recuero, Manuel; Cantarero Carmona, Alejandro; Redondo, Alberto J.; Sánchez Sánchez, Nuria; Redondo, Tomás
Some theoretical models predict that nestling begging must be a costly activity to transmit reliable information to parents about offspring hunger. One candidate cost is oxidative stress, which could impair growth or immune function. This hypothesis predicts that nestlings in a poor oxidative status should pay higher costs for a given amount of begging, hence beg at lower rates for a similar degree of hunger. To test this prediction, we experimentally induced a transient alteration of the oxidative status of Barn Swallow (Hirundo rustica) nestlings by administering them either a single dose of a pro-oxidant substance (BSO) or an antioxidant (vitamin E), and recorded their begging behaviour. Chicks treated with vitamin E increased their begging rates in the hours following treatment. However, and contrary to prediction, BSO nestlings did not decrease begging compared to controls. These inconsistent results are difficult to explain by the hypothesis that begging performance is mediated by the oxidative status of nestlings because chicks with an impaired oxidative status (the BSO group) begged the same as controls. Alternatively, vitamin E may alleviate the inhibitory effects on begging of stress responses mediated by glucocorticoids. This study suggests that oxidative costs of begging are minor or absent in this species. Future studies should consider alternative explanations for an association between oxidative status, antioxidant supplementation and begging behaviour, particularly those involving glucocorticoids.
Animal Research in Spain: A Study of Public Perception and Attitudes
(Animals, 2023) Miguel-Batuecas, Andrea; Fuertes Recuero, Manuel; Díaz-Regañón Fernández, David Rafael; Ortiz Díez, Gustavo; De Pablo Moreno, Juan Andrés
Since the time of Hippocrates in the 4th century BC, animal research has been extensively used for various purposes up to the present day. However, the use of animals for research has also been controversial for a long time. We report the findings of a public, online questionnaire-based survey designed to assess the opinions of a sample of Spanish society regarding animal research. Demographic data and opinions were obtained from 806 respondents. The results indicated a high level of acceptance of animal research (73.1%). However, certain factors, such as completing the questionnaire immediately after a reading negative media report (OR = 2.41; 95%CI: 1.64–3.54; p < 0.001), being a woman (OR = 1.77; 95%CI: 1.24–2.53; p = 0.002) or having a non-scientific background (OR = 2.47; 95%CI: 1.76–3.47; p < 0.001), were associated with a tendency towards a more negative opinion. The opinions seemed to be influenced by gender, education level and by protest incidents reported in the media. Our results also indicate that a lot of information regarding animal welfare, such as care and handling protocols, along with legislation was unknown to individuals. Further, a growing popularity of companion species and opposition to animal experimentation for non-biomedical purposes were reflected in the responses obtained. The use of animals for research purposes emerged as a sensitive social issue in terms of concerns about animal ethics and welfare.
Project number: 244
Elaboración y desarrollo de materiales accesibles para la formación de jóvenes con discapacidad intelectual en la atención y cuidado de animales, mediante la intervención en el Proyecto Liceo
(2022) Encinas Cerezo, María Teresa; Pablo Moreno, Juan Andrés de; Águeda Gómez, Alejandra de; De Las Heras Molina, Ana; Fernández Bravo, Juan Antonio; Fernández de Lys Galván, Gonzalo; Fuertes Recuero, Manuel; Gilabert Santos, Juan Antonio; Marín García, María Del Pilar; Morón Elorza, Pablo; Nieto González, David; Olivos Ore, Luis Alcides; Ortega Moreno, Carlos; Barbacid Yela, Andrea; Maqueda Olivas, Clara María; Arribas Blázquez, Marina; Díez Martín, Amalia
Profesionales especializados en Veterinaria y Educación Especial desarrollarán materiales cognitivamente accesibles para jóvenes con discapacidad intelectual aplicables a los contenidos del itinerario de Cuidado de Animales del Proyecto Liceo.
Proneurogenic actions of follicle-stimulating hormone on neurospheres derived from ovarian cortical cells in vitro
(BMC Veterinary Research, 2024) González Gil, Alfredo; Sánchez Maldonado, María Belén; Rojo Salvador, Concepción; Flor García, Miguel; Queiroga, Felisbina Luisa; Ovalle, Susana; Ramos Ruiz, Ricardo; Fuertes Recuero, Manuel; Picazo González, Rosa Ana
Background: Neural stem and progenitor cells (NSPCs) from extra-neural origin represent a valuable tool for autologous cell therapy and research in neurogenesis. Identification of proneurogenic biomolecules on NSPCs would improve the success of cell therapies for neurodegenerative diseases. Preliminary data suggested that follicle-stimulating hormone (FSH) might act in this fashion. This study was aimed to elucidate whether FSH promotes development, self-renewal, and is proneurogenic on neurospheres (NS) derived from sheep ovarian cortical cells (OCCs). Two culture strategies were carried out: (a) long-term, 21-days NS culture (control vs. FSH group) with NS morphometric evaluation, gene expression analyses of stemness and lineage markers, and immunolocalization of NSPCs antigens; (b) NS assay to demonstrate FSH actions on self-renewal and differentiation capacity of NS cultured with one of three defined media: M1: positive control with EGF/FGF2; M2: control; and M3: M2 supplemented with FSH. Results: In long-term cultures, FSH increased NS diameters with respect to control group (302.90 ± 25.20 μm vs. 183.20 ± 7.63 on day 9, respectively), upregulated nestin (days 15/21), Sox2 (day 21) and Pax6 (days 15/21) and increased the percentages of cells immunolocalizing these proteins. During NS assays, FSH stimulated NSCPs proliferation, and self-renewal, increasing NS diameters during the two expansion periods and the expression of the neuron precursor transcript DCX during the second one. In the FSH-group there were more frequent cell-bridges among neighbouring NS. Conclusions: FSH is a proneurogenic hormone that promotes OCC-NSPCs self-renewal and NS development. Future studies will be necessary to support the proneurogenic actions of FSH and its potential use in basic and applied research related to cell therapy.
Hatching asynchrony as a reproductive strategy in birds may explain the hatching failure of the last eggs of the clutch
(Ibis: International Journal of Avian Science, 2024) Fuertes Recuero, Manuel; Baldan, David; Cantarero Carmona, Alejandro
Hatching failure, due to infertility or embryo mortality, is an important factor contributing to reduced reproductive success in birds. Although hatching failure and its possible causes have been widely investigated, the stage of development at which embryo death occurs and its association with laying order have rarely been studied. The relative size of eggs laid in different positions within the laying order is a key factor that can lead to different parental reproductive strategies, such as hatching asynchrony. Here we investigate hatching failure in relation to laying order by establishing the developmental stage of dead embryos found in unhatched European Pied Flycatcher Ficedula hypoleuca eggs and considering possible causes of failure. We found that variation in egg dimensions showed a quadratic relationship with laying order, with relatively large volumes and sizes in the first and last positions of the clutch. Egg position in the laying sequence was also related to hatching failure, with the first and last positions being more susceptible to failure. The death of embryos late in development was more likely for eggs laid later in the sequence. To our knowledge, this is the first study showing that females may be adaptively allocating more resources to last-laid eggs to avoid competitive disadvantages between siblings, a strategy that seems to fail becasue these eggs suffer greater embryo mortality.
Successful ultrasound-guided ovum pick-up (OPU) and subsequent in vitro embryo production in a domestic cat
(Theriogenology, 2024) Priego González, Andrea; Muñoz Maceda, Ana; Cerdeira Lozano, Joaquín Vicente; Fominaya García, Hernán Luis; Fuertes Recuero, Manuel; Ortiz Díez, Gustavo; Gardoqui Arias, Manuel; Roldán, Eduardo R. S.; Sánchez Calabuig, María Jesús
Ovum Pick Up (OPU) is a minimally invasive technique widely used in cattle and mares for oocyte retrieval, involving ultrasound-guided puncture of ovarian follicles. It has been demonstrated that this technique is safe for its repeated use in the same female without affecting her reproductive health, allowing for the retrieval of oocytes in individuals regardless of their reproductive status. The oocytes obtained through OPU can subsequently be used for in vitro embryo production (IVP) using assisted reproductive techniques (ARTs) or be cryopreserved in biobanks for their future use. Traditionally, the minimally invasive technique of choice performed in vivo in domestic and wild felines was LOPU (laparoscopic-guided ovum pick up). The present study was designed to explore if ultrasound-guided OPU in the domestic cat is safe and effective. In an initial series of ex vivo experiments (n = 92 ovaries, n = 434 oocytes), the effect of different aspiration pressures for oocyte collection was explored. These experiments identified 43 mmHg as the optimal aspiration pressure, resulting in the highest recovery rate and a favorable maturation and blastocyst rate. Subsequently, 16 grade I and II oocytes were retrieved by OPU and 101 oocytes were retrieved following ovariectomy and slicing. Sixteen oocytes obtained with each technique were subjected to in vitro maturation (IVM) and in vitro fertilization (IVF). A total of 14 presumptive zygotes were selected for in vitro culture (IVC) from each group (OPU and slicing), obtaining a cleavage rate of 57.1 % and 64.2 %, a morula rate of 28.5 % in both groups, and a blastocyst rate of 7.14 % and 14.2 % respectively. The hormonal stimulation protocol was well-tolerated, with no adverse effects observed. Moreover, no complications arose during the ovariectomy performed post-OPU. The use of this technique in domestic cats represents a significant step forward in terms of safety, replicability, and invasiveness, serving as a valuable model for its application in wild felids species. Additional research involving a greater number of animals is required to validate these encouraging findings.
Project number: PIMCD257/23-24
Colocación y seguimiento de cajas nido en el entorno natural de la Facultad de Veterinaria de la UCM, aplicable a una enseñanza teórico-práctica del campo de la Zoología.
(2024) Fontanillas Pérez, Juan Carlos; Aranda García, Enrique; Cantarero Carmona, Alejandro; Fuertes Recuero, Manuel; García-Cuenca Ariati, Isabel; González Gil, Alfredo; Jerónimo Sánchez, Benedicto; López Arrabe, Jimena; Martínez Sainz, María Del Pilar; Picazo González, Rosa Ana; Sanz Guijarro, Irene
El medio natural en la actualidad es desconocido por una gran parte del alumnado, por lo que puede constituir uno de los principales recursos de aprendizaje, permitiendo la observación de los fenómenos naturales y el contacto directo con los seres vivos. La falta de contacto con la naturaleza en general está siendo sustituida por una oferta de realidad virtual. A su vez, se están produciendo cambios globales de forma acelerada en todo el planeta debido a la contaminación, la cual está elevando la temperatura de la Tierra y cambiando el ritmo estacional, lo que se ha traducido en la pérdida de hábitats naturales, la subida del nivel de las aguas y a la desaparición de decenas de especies animales. Con el objetivo de hacer frente a este hecho, y poder aportar nuestro grano de arena al cambio, realizamos este proyecto en el que retamos a nuestro alumnado a mitigar estas repercusiones mediante la colocación y el seguimiento de cajas nido en los alrededores de la Facultad de Veterinaria. Este proyecto se ha ajustado a la Agenda 2030, donde la UNESCO pone de relieve diferentes objetivos sostenibles como fueron “Educación de Calidad”, “Ciudades y Comunidades sostenibles”, y “Vida de Ecosistemas Terrestres”. Con el presente proyecto hemos puesto en contacto directo al alumnado con los fenómenos naturales y la vida silvestre, enseñando y generando comportamientos proambientales, conciencia sobre aspectos de la conservación ambiental y conocimiento científico. Además, ha proporcionado sesiones prácticas, las cuales son una estrategia de enseñanza clave en el Grado de Veterinaria, donde los estudiantes han llevado a cabo procedimientos, y han obtenido habilidades y destrezas específicas relacionadas con las aves insectívoras urbanas Se han colocado 60 cajas nidos en las que se han instalado distintas especies de aves paseriformes. Los estudiantes de Grado en Veterinaria han desempeñado un papel fundamental en la puesta a punto de las cajas nido y en su posterior seguimiento. En las cajas nido colocadas han completado su reproducción durante la primavera y verano de 2023, tres parejas de Herrerillo común y cuatro parejas de Gorrión molinero. Se han obtenido parámetros reproductivos como fecha y tamaño de puesta, fecha de eclosión de los pollos, número de volantones producidos, etc. y se ha procedido a anillar a 45 polluelos. Estos, junto con otros datos de carácter científico, como medidas biométricas, se han incorporado a un fichero de datos para su uso en futuros trabajos científicos que se puedan lleven a cabo. Además, el proyecto ha tenido tal alcance que la Comunidad de Madrid mediante el proyecto Arco Verde ha querido participar activamente. También ha servido para impartir charlas sobre diversidad urbana mediante una ponencia titulada “Cajas nido para reproducción e investigación de aves urbanas”, y para participar en la Feria del Campo mediante la realización de un taller titulado “Aves de nuestro entorno: una mirada al interior del nido.”