Person: Sánchez-Vizcaíno Rodríguez, José Manuel
Loading...
First Name
José Manuel
Last Name
Sánchez-Vizcaíno Rodríguez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
94 results
Search Results
Now showing 1 - 10 of 94
Item The Role of Interleukine-10 and Interferon-γ as Potential Markers of the Evolution of African Swine Fever Virus Infection in Wild Boar(Pathogens, 2021) Barroso Arévalo, Sandra; Sánchez-Vizcaíno Rodríguez, José Manuel; Cadenas Fernández, Estefanía; Barasona García-Arévalo, José ÁngelAfrican swine fever virus (ASFv) is one of the most challenging pathogens to affect both domestic and wild pigs. The disease has now spread to Europe and Asia, causing great damage to the pig industry. Although no commercial vaccine with which to control the disease is, as yet, available, some potential vaccine candidates have shown good results in terms of protection. However, little is known about the host immune mechanisms underlying that protection, especially in wild boar, which is the main reservoir of the disease in Europe. Here, we study the role played by two cytokines (IL-10 and IFN-γ) in wild boar orally inoculated with the attenuated vaccine candidate Lv17/WB/Rie1 and challenged with a virulent ASFv genotype II isolate. A group of naïve wild boar challenged with the latter isolate was also established as a control group. Our results showed that both cytokines play a key role in protecting the host against the challenge virus. While high levels of IL-10 in serum may trigger an immune system malfunctioning in challenged animals, the provision of stable levels of this cytokine over time may help to control the disease. This, together with high and timely induction of IFN-γ by the vaccine candidate, could help protect animals from fatal outcomes. Further studies should be conducted in order to support these preliminary results and confirm the role of these two cytokines as potential markers of the evolution of ASFV infection.Item Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions(Viruses, 2021) Vargas Castro, Ignacio; Melero, Mar; Crespo-Picazo, José Luis; Jiménez Martínez, María De Los Ángeles; Sierra, Eva; Rubio-Guerri, Consuelo; Arbelo, Manuel; Fernández, Antonio; García-Párraga, Daniel; Sánchez-Vizcaíno Rodríguez, José ManuelThe monitoring of herpesvirus infection provides useful information when assessing marine mammals’ health. This paper shows the prevalence of herpesvirus infection (80.85%) in 47 cetaceans stranded on the coast of the Valencian Community, Spain. Of the 966 tissues evaluated, 121 tested positive when employing nested-PCR (12.53%). The largest proportion of herpesvirus-positive tissue samples was in the reproductive system, nervous system, and tegument. Herpesvirus was more prevalent in females, juveniles, and calves. More than half the DNA PCR positive tissues contained herpesvirus RNA, indicating the presence of actively replicating virus. This RNA was most frequently found in neonates. Fourteen unique sequences were identified. Most amplified sequences belonged to the Gammaherpesvirinae subfamily, but a greater variation was found in Alphaherpesvirinae sequences. This is the first report of systematic herpesvirus DNA and RNA determination in free-ranging cetaceans. Nine (19.14%) were infected with cetacean morbillivirus and all of them (100%) were coinfected with herpesvirus. Lesions similar to those caused by herpesvirus in other species were observed, mainly in the skin, upper digestive tract, genitalia, and central nervous system. Other lesions were also attributable to concomitant etiologies or were nonspecific. It is necessary to investigate the possible role of herpesvirus infection in those cases.Item Be-FAST – Between Farm Animal Spatial Transmission: An epidemiological model for studying the spread and the economic impact of animal diseases(2013) Ivorra, Benjamín Pierre Paul; Fernández Carrión, Eduardo; Martínez López, Beatriz; Sánchez-Vizcaíno Rodríguez, José Manuel; Ramos del Olmo, Ángel ManuelItem Mathematical formulation and validation of the Be-FAST model for Classical Swine Fever Virus spread between and within farms(Annals of operations research, 2014) Ivorra, Benjamín Pierre Paul; Martínez López, Beatriz; Sánchez-Vizcaíno Rodríguez, José Manuel; Ramos del Olmo, Ángel ManuelClassical Swine Fever is a viral disease of pigs that causes severe restrictions on the movement of pigs and pig products in the affected areas. The knowledge of its spread patterns and risk factors would help to implement specific measures for controlling future outbreaks. In this article, we describe in detail a spatial hybrid model, called Be-FAST, based on the combination of a stochastic Individual-Based model (modeling the interactions between the farms, considered as individuals) for between-farm spread with a Susceptible-Infected model for within-farm spread, to simulate the spread of this disease and identify risk zones in a given region. First, we focus on the mathematical formulation of each component of the model. Then, in order to validate Be-FAST, we perform various numerical experiments considering the Spanish province of Segovia. Obtained results are compared with the ones given by two other Individual-Based models and real outbreaks data from Segovia and The NetherlandsItem Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar(Pathogens, 2020) Cadenas Fernández, Estefanía; Sánchez-Vizcaíno Rodríguez, José Manuel; Kosowska, Aleksandra; Rivera Arroyo, Belén; Mayoral Alegre, Francisco José; Rodríguez Bertos, Antonio Manuel; Yao, Jianxiu; Bray, Jocelyn; Lokhandwala, Shehnaz; Mwangi, Waithaka; Barasona García-Arévalo, José ÁngelAfrican swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insufficient and an effective vaccine against ASFV needs to be urgently developed. We hypothesized that immunization with a cocktail of thirty-five rationally selected antigens would improve the protective efficacy of subunit vaccine prototypes given that the combination of fewer immunogenic antigens (between 2 and 22) has failed to elicit protective efficacy. To this end, immunogenicity and efficacy of thirty-five adenovirus-vectored ASFV antigens were evaluated in wild boar. The treated animals were divided into different groups to test the use of BioMize adjuvant and different inoculation strategies. Forty-eight days after priming, the nine treated and two control wild boar were challenged with the virulent ASFV Arm07 isolate. All animals showed clinical signs and pathological findings consistent with ASF. This lack of protection is in line with other studies with subunit vaccine prototypes, demonstrating that there is still much room for improvement to obtain an effective subunit ASFV vaccine.Item First Oral Vaccination of Eurasian Wild Boar Against African Swine Fever Virus Genotype II(Frontiers in Veterinary Science, 2019) Gallardo, Carmina; Arias, Marisa; Barasona García-Arévalo, José Ángel; Cadenas Fernández, Estefanía; Jurado Díaz, Cristina; Rivera Arroyo, Belén; Rodríguez Bertos, Antonio Manuel; Sánchez-Vizcaíno Rodríguez, José ManuelAfrican swine fever (ASF), the most significant threat to the pig industry worldwide, has spread to more than 55 countries on three continents, and it affects more than 77% of the world swine population. In the European Union, wild boar (Sus scrofa) is the most severely affected host. The main reasons for the unprecedented and constant spread of ASF in Europe are the trade activities, the continuous movement of infected-wild boar populations among regions and the lack of vaccine to prevent ASF infection. In this study, we demonstrate that oral immunization of wild boar with a non-hemadsorbing, attenuated ASF virus of genotype II isolated in Latvia in 2017 (Lv17/WB/Rie1) conferred 92% protection against challenge with a virulent ASF virus isolate (Arm07). This is, to our knowledge, the first report of a promising vaccine against ASF virus in wild boar by oral administration. Further studies should assess the safety of repeated administration and overdose, characterize long-term shedding and verify the genetic stability of the vaccine virus to confirm if Lv17/WB/Rie1 could be used for free-ranging wild boar in ASF control programs.Item Reproductive ratio for the local spread of highly pathogenic avian influenza in wild bird populations of Europe, 2005–2008(Epidemiology and Infection, 2011) Iglesias Martín, Irene; Pérez, A. M.; Sánchez-Vizcaíno Rodríguez, José Manuel; Muñoz Reoyo, María Jesús; Martínez Avilés, Marta; De la Torre, AnaHighly pathogenic avian influenza (HPAI) has devastating consequences for the poultry industry of affected countries. Control of HPAI has been impaired by the role of wildlife species that act as disease reservoirs and as a potential source of infection for domestic populations. The reproductive ratio (R0) of HPAI was quantified in nine clusters of outbreaks detected in wild birds in Europe (2005–2008) for which population data were not available. The median value of R0 was similar (1. 1–3. 4) for the nine clusters and it was about tenfold smaller than the value estimated for poultry in The Netherlands in 2003. Results presented here will be useful to parameterize models for spread of HPAI in wild birds and to design effective prevention programmes for the European poultry sector. The method is suitable to estimate R0 in the absence of population data, which is a condition typically observed for many wildlife and certain domestic species and systems.Item Characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep(TheScientificWorldJournal, 2012) Pérez de Diego, Ana Cristina; Sánchez Cordón, Pedro José; Heras, Ana Isabel de las; Sánchez-Vizcaíno Rodríguez, José ManuelThe protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes.Item Quantitative risk assessment of African swine fever introduction into Spain by legal import of swine products(Research in Veterinary Science, 2023) Muñoz Pérez, Carolina; Martínez López, Beatriz; Gómez Vázquez, José Pablo; Aguilar Vega, Cecilia; Bosch López, Jaime Alfonso; Ito, Satoshi; Martínez Avilés, Marta; Sánchez-Vizcaíno Rodríguez, José ManuelAfrican swine fever (ASF) is currently threatening the global swine industry. Its unstoppable global spread poses a serious risk to Spain, one of the world's leading producers. Over the past years, there has been an increased global burden of ASF not only in swine but also swine products. Unfortunately, many pigs are not diagnosed before slaughter and their products are used for human consumption. These ASF-contaminated products are only a source for new ASF outbreaks when they are consumed by domestic pigs or wild boar, which may happen either by swill feeding or landfill access. This study presents a quantitative stochastic risk assessment model for the introduction of ASF into Spain via the legal import of swine products, specifically pork and pork products. Entry assessment, exposure assessment, consequence assessment and risk estimation were carried out. The results suggest an annual probability of ASF introduction into Spain of 1.74 × 10−4, the highest risk being represented by Hungary, Portugal, and Poland. Monthly risk distribution is homogeneously distributed throughout the year. Illegal trade and pork product movement for own consumption (e.g., air and ship passenger luggage) have not been taken into account due to the lack of available, accredited data sources. This limitation may have influenced the model's outcomes and, the risk of introduction might be higher than that estimated. Nevertheless, the results presented herein would contribute to allocating resources to areas at higher risk, improving prevention and control strategies and, ultimately, would help reduce the risk of ASF introduction into Spain.Item Modeling classical swine fever spread using a spatial hybrid model(XXI Congreso de Ecuaciones Diferenciales y Aplicaciones (CEDYA).Ciudad Real, 21-25 septiembre 2009, 2009) Ivorra, Benjamín Pierre Paul; Martínez López, Beatriz; Ramos del Olmo, Ángel Manuel; Sánchez-Vizcaíno Rodríguez, José ManuelClassical swine fever (CSF) is a highly contagious viral disease of pigs and wild boars that causes severe commercial restrictions to the affected countries. The knowledge of spread patterns and risk factors that are involved in the transmission of CSF would help to implement specific measures and to reduce the disease spread in future outbreaks. In this article, we introduce a new spatial hybrid model developed for the spread of CSF. It is based on the combination of a stochastic individual Based model with a Susceptible-Infected model. The coefficients and parameters of the models are estimated using real data.