Person: Navarro Villoslada, Fernando
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First Name
Fernando
Last Name
Navarro Villoslada
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
Identifiers
12 results
Search Results
Now showing 1 - 10 of 12
Item Magnetic microbeads-based amperometric immunoplatform for the rapid and sensitive detection of N6-methyladenosine to assist in metastatic cancer cells discrimination(Biosensors and Bioelectronics, 2021) Povedano Muñumel, Eloy; Gamella Carballo, María; Torrente Rodríguez, Rebeca Magnolia; Montero-Calle, Ana; Pedrero Muñoz, María; Solís-Fernández, Guillermo; Navarro Villoslada, Fernando; Barderas, Rodrigo; Campuzano Ruiz, Susana; Pingarrón, José; Pingarrón Carrazón, José ManuelThis work describes the preparation of an immunoplatform for the sensitive and selective determination of N6-methyladenosine (m6A). The simple and fast protocol involves for the first time the use of micromagnetic immunoconjugates to establish a direct competitive assay between the m6A target and a biotinylated RNA oligomer bearing a single m6A enzymatically labelled with a commercial conjugate of streptavidin-peroxidase (Strep-HRP) as tracer. The cathodic current change measured in the presence of H2O2/hydroquinone (HQ) at screen-printed carbon electrodes (SPCEs) upon surface capturing the magnetic bioconjugates is inversely proportional to the m6A target concentration. After evaluating the effect of key variables, the analytical characteristics were established for the determination of three different targets: the N6-methyladenosine-5′ -triphosphate (m6ATP) ribonucleotide, a short synthetic RNA oligomer bearing a single m6A and the positive control provided in a commercial colorimetric kit for m6A-RNA quantification. The obtained results show that this immunoplatform is competitive with other methods reported to date, achieving an improved sensitivity (limit of detection of 0.9 pM for the short synthetic oligomer) using a much simpler and faster protocol (~1 h) and disposable electrodes for the transduction. Furthermore, the applicability for discriminating the metastatic potential of cancer cells by directly analyzing a small amount of raw total RNA without enriching or fragmenting was also preliminary assessed.- Project number: 94
Item La química da mucho juego...¿jugamos?(2017) León González, María Eugenia; Aragoncillo Abádanes, Cristina; Luna Costales, Amparo; Martínez del Campo, Teresa; Rodríguez Yunta, María Josefa; Manuel de Villena Rueda, Francisco Javier; Navarro Villoslada, Fernando; Pérez Arribas, Luis Vicente; Blanco Asenjo, Miriam; Ballesteros de León, Elisa; Gómez Gómez, Beatriz; Moreno Casillas, Gustavo¿Quién no ha jugado alguna vez? ¿Qué juegos conocemos? ¿Podemos motivar a los alumnos a través del juego?. Desde nuestra experiencia durante el curso 2016-2017 y utilizando adaptaciones de dos juegos tradicionales de mesa podemos observar que los alumnos participan y aprenden jugando y que el profesor puede utilizar el juego para evaluar las competencias y habilidades de los participantes. Con el juego los docentes dejamos de ser el centro de la clase o del laboratorio y pasamos a ser meros conductores del proceso de aprendizaje además de facilitar el trabajo en pequeños grupos. Los juegos utilizados son una adaptación de juegos de mesa que utilizan preguntas relacionadas con la Química. Se ha desarrollado material didáctico en forma de cartulinas con cuatro tipos de preguntas adaptadas a diferentes asignaturas de diferentes grados y que se pueden utilizar tanto en el laboratorio de prácticas como en el aula. Los juegos se han utilizado en asignaturas de los grados de Química, Biología e Ingeniería Química en tutorías, seminarios o en el laboratorio o bien al final del mismo o durante los periodos en los que los alumnos tienen “tiempos muertos” dado el desarrollo de las prácticas. - Project number: 127
Item Diseño y preparación de un laboratorio virtual de Química Analítica: Técnicas instrumentales de análisis(2022) Benito Peña, Elena; Burgueño Arjona, Maria José; Campuzano Ruiz, Susana; Madrid Albarrán, María Yolanda; Marazuela Lamata, María Dolores; Mateos Briz, María Raquel; Moreno Bondi, María Cruz; Navarro Villoslada, Fernando; Pedrero Muñoz, María; Reviejo García, Ángel Julio; Tortajada Pérez, José; Urraca Ruiz, Javier; Villalonga Santana, Reynaldo; Blanco Asenjo, Miriam; del Valle Ávila, Marcos; Molla Escudero, David; Navarro Duro, Marina; del Rosario García-Marcos, Beatriz; Merino Sierra, Miguel Ángel; García López, PatriciaLa reciente pandemia del COVID-19 ha supuesto un cambio excepcional y drástico de la concepción tradicional del aprendizaje, tanto para los estudiantes como para los docentes. Ante esta situación se requiere no sólo acciones que faciliten la adaptación de los estudiantes y profesores a las plataformas educativas en línea, sino también a que éstas se conviertan en auténticas herramientas para potenciar y mejorar de forma significativa el aprendizaje del alumno. El presente proyecto de Innovación Educativa y Mejora de la Calidad Docente pretende mejorar la calidad del aprendizaje de varias asignaturas de los Grados de Química e Ingeniería Química que llevan asociado un Laboratorio de Técnicas Instrumentales. Tradicionalmente esa docencia práctica ha sido presencial, pero la situación excepcional surgida con la pandemia en el curso 2019/2020, ha demostrado que disponer de unas prácticas virtualizadas es de gran ayuda para facilitar el aprendizaje de los alumnos y facilitar una transferencia del conocimiento constructivo y colaborativo. Desde hace años, el personal docente e investigador (PDI), personal de administración y servicios (PAS) y los estudiantes de la UCM, disponemos de la plataforma de enseñanza online Moodle (Campus Virtual, CV). Sin embargo, la pandemia que vivimos ha evidenciado la brecha digital en lo referente a cómo usar Moodle y, por ende, la utilización de las Tecnologías de Información y Comunicación (TICs) como facilitadoras didácticas. A pesar de nuestras limitaciones, tanto profesores como estudiantes, hemos desarrollado una enorme capacidad resiliente, lo que permitió, durante el pasado mes de mayo, la puesta en marcha de los primeros laboratorios en línea en el Departamento de Química Analítica de la UCM. Fruto de esta experiencia, así como de la situación actual de incertidumbre para el curso próximo, algunos profesores, estudiantes y PAS del departamento hemos decidido adelantarnos a un escenario futuro en el que se contemple nuevamente la impartición de Docencia Experimental en línea y solicitar el presente proyecto de Innovación Educativa y Mejora de la Calidad Docente. Item Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads(Food Chemistry, 2023) Moya-Cavas, Tamara; Navarro Villoslada, Fernando; Urraca Ruiz, Javier; Antonio Serrano, Luis; Orellana Moraleda, Guillermo; Moreno-Bondi, María Cruz; Moreno Bondi, María CruzThis work reports the optimization of a method using Molecularly Imprinted Polymers (MIPs) for the simultaneous determination of zearalenone and alternariol mycotoxins. The method was optimized using a chemometric approach where in the optimized conditions, the cartridges with a mixture (50:50, w/w) of both MIPs, were loaded with 30 mL of sample, washed with 2 mL of ACN/water (20/80, v/v) and eluted with 2.5 mL of trifluoroacetic acid/MeOH (3/97, v/v). The extracts were analyzed by HPLC coupled to a fluorescence detector (FLD). The optimized method has been applied and validated to the analysis of the mycotoxins in maize, sunflower and olive oils samples with a limit of detection of 5 and 2 μg kg- 1, respectively. Recoveries were in the range of 94 % to 108 % (RSD < 6 %) for zearalenone and 92 % to 113 % (RSD < 5 %) for alternariol. The results were confirmed by HPLC-MS/MS.Item Extracting mycotoxins from edible vegetable oils by using green, ecofriendly deep eutectic solvents(Food Chemistry, 2023) Pradanas-González, Fernando; Aragoneses-Cazorla, Rubén; Ángel Merino-Sierra, Miguel; Andrade-Bartolomé, Elena; Navarro-Villoslada, Fernando; Benito-Peña, Elena; Moreno-Bondi, María Cruz; Moreno Bondi, María Cruz; Benito Peña, María Elena; Navarro Villoslada, Fernando; Prádanas González, FernandoIn this work, we developed an environmentally friendly liquid–liquid microextraction method using a natural deep eutectic solvent in combination with liquid chromatography for the simultaneous determination of four mycotoxins (deoxynivalenol, alternariol, ochratoxin A and zearalenone) in edible vegetable oils. A chemometric approach assessed the effect of the operational parameters on the mycotoxin extraction efficiency. The extracts were analyzed by HPLC coupled with a diode array and fluorescence detector. The optimum NADES composition resulted in the highest extraction recoveries, and it was applied to coextract the target mycotoxins in several types of edible vegetable oils without using hazardous solvents or requiring further clean-up. The limits of detection ranged from 0.07 to 300μg/kg, and recoveries were close to 100%, except for zearalenone(viz. 35%), with relative standard deviations below 9% in all cases. The proposed method was validated following the European Commission 2002/657/EC and 2006/401/EC.Item The ultratrace determination of fluoroquinolones in river water samples by an online solid-phase extraction method using a molecularly imprinted polymer as a selective sorbent(Molecules, 2022) Baeza, Ana; Urriza-Arsuaga, Idoia; Navarro Villoslada, Fernando; Urraca Ruiz, JavierFluoroquinolones (FQs) are broad-spectrum antibiotics widely used to treat animal and human infections. The use of FQs in these activities has increased the presence of antibiotics in wastewater and food, triggering antimicrobial resistance, which has severe consequences for human health. The detection of antibiotics residues in water and food samples has attracted much attention. Herein, we report the development of a highly sensitive online solid-phase extraction methodology based on a selective molecularly imprinted polymer (MIP) and fluorescent detection (HPLC-FLD) for the determination of FQs in water at low ng L−1 level concentration. Under the optimal conditions, good linearity was obtained ranging from 0.7 to 666 ng L−1 for 7 FQs, achieving limits of detection (LOD) in the low ng L−1 level and excellent precision. Recoveries ranged between 54 and 118% (RSD < 17%) for all the FQs tested. The method was applied to determining FQs in river water. These results demonstrated that the developed method is highly sensitive and selective.Item Calibración multivariante para la determinación de clorofenoles contaminantes prioritarios mediante espectrofotometría de diodos integrados y FIA(2003) Navarro Villoslada, Fernando; Pérez Arribas, Luis VicenteLa presente memoria se centra en la aplicación de métodos extractivo-espectrofotometricos para la determinación de clorofenoles contaminantes prioritarios, 2-clorofenol, 4-cloro-3-metilfenol, 2,4-diclorofenol, 2,4,6-triclorofenol y pentaclorofenol. Se emplean sales de amonio cuaternario, las cuales favorecen la extracción de los clorofenoles. La determinación simultanea se realizo mediante la aplicación de diferentes métodos de calibración multivariante de primer orden como regresión clásica de mínimos cuadrados (cls), regresión inversa de mínimos cuadrados (ils), filtro de kalman (kf), regresión de componentes principales (pcr) y regresion parcial de mínimos cuadrados (pls). Se estudia la automatización del método mediante fia, y se aplica a la determinación de clorofenoles contaminantes prioritarios en muestras de agua; estudiándose también su determinación en matrices muy complejas como orina humanaItem Fast and straightforward synthesis in molecular imprinting: core–shell polymerization of magnetic imprinted polymers by microwave induction(ACS Applied Polymer Materials, 2024) Guadaño-Sánchez, Miriam; Navarro Villoslada, Fernando; Delgado-Soria, Guiomar; Marco, José F.; Saura Múzquiz, Matilde; Álvaro-Gómez, Laura; Presa Muñoz De Toro, Patricia Marcela De La; Pérez García, Lucas; Lucas Urraca, JavierIn this work, we report a simple and effective approach for preparing molecular imprint polymer (MIP) coatings directly on magnetic multicore (MMC) iron oxide nanoparticles using a microwave reactor to trigger the polymerization reaction. The nanoparticles were manufactured using a microwave reactor, and the MIPs were synthesized with rhodamine 6G (R6G) as the template molecule, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker. The produced MMCs and MMC-MIPs were extensively characterized by analysis of powder X-ray diffraction data, Fourier transform infrared spectra, Mossbauer spectroscopy, SQUID magnetometry, and transmission electron microscopy (TEM). The results show the effective formation of the polymeric layer on the surface of the MMCs, without alteration of the structure or physical properties of the core magnetic nanoparticles. The synthesized MIPs presented a high efficiency in the imprinting process of the MMC-MIPs and high selectivity (MIP R6G, Ka = 7.31 × 10–2 M–1 y, 𝑁¯= 14.14 μmol g–1) toward the target molecule R6G.Item Determination of the oxygen permeability (Dk) of contact lenses with a fiber-optic luminescent sensor system(Sensors and Actuators B - Chemical, 2007) Pérez Ortíz, Noelia; Navarro Villoslada, Fernando; Orellana Moraleda, Guillermo; Moreno Jiménez, FlorencioAn optical sensing system, alternative to current electrochemical techniques, has been developed for determining the oxygen permeability (Dk) of rigid gas-permeable contact lenses. The novel sensor is based on kinetic measurements of the oxygen partial pressure inside a chamber sealed by the sample contact lens, where a thin luminescent O2-sensitive film is placed. The standard equation for calculating the Dk value of the lens material has been adapted to the optical technique and a fitting procedure of the sensor response has been developed to achieve the maximum accuracy and precision. Unlike the electrochemical techniques, the optical sensor is unaffected by the contact lens thickness and the lens edge and boundary effects. Therefore, the latter does not require lengthy measuring procedures for extracting the actual Dk values. The optical sensor system has been applied to oxygen permeability determinations of commercial contact lenses of nominal Dk values equal to 17 and 36 ANSI units of 10−11 cm3 cm/s cm2 mmHg.Item Versatile electroanalytical bioplatforms for dimultaneous determination of cancer-related DNA 5-hethyl- and 5-hydroxymethyl-cytosines at global and gene-specific levels in human serum and tissues(ACS Sensors, 2018) Povedano Muñumel, Eloy; Ruiz Valdepeñas Montiel, Víctor; Valverde De La Fuente, Alejandro; Navarro Villoslada, Fernando; Yáñez-Sedeño Orive, Paloma; Pedrero Muñoz, María; Montero-Calle, Ana; Barderas Manchado, Rodrigo; Peláez-García, Alberto; Mendiola, Marta; Hardisson, David; Feliú, Jaime; Camps, Jordi; Rodríguez-Tomàs, Elisabet; Joven, Jorge; Arenas, Meritxell; Campuzano Ruiz, Susana; Pingarrón Carrazón, José ManuelThis paper reports the preparation of versatile electrochemical biosensing platforms for the simple, rapid, and PCR-independent detection of the most frequent DNA methylation marks (5-methylcytosine, 5-mC, and/or 5-hydroxymethylcytosine, 5-hmC) both at global and gene-specific levels. The implemented strategies, relying on the smart coupling of immuno-magnetic beads (MBs), specific DNA probes and amperometric detection at screen-printed carbon electrodes (SPCEs), provided sensitive and selective determination of the target methylated DNAs in less than 90 min with a great reproducibility and demonstrated feasibility for the simultaneous detection of the same or different cytosine epimarks both at global level and in different loci of the same gene or in different genes. The bioplatforms were applied to determine global methylation events in paraffin-embedded colorectal tissues and specific methylation at promoters of tumor suppressor genes in genomic DNA extracted from cancer cells and paraffin-embedded colorectal tissues, and in serum without previous DNA extraction from cancer patients.