Person: Gómez Villafuertes, María Rosa
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First Name
María Rosa
Last Name
Gómez Villafuertes
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
- PublicationChanges in melatonin receptor expression in a murine model of glaucoma(2020-07-29) Martínez Águila, Alejandro; Fonseca Vázquez, Begoña; Pérez de Lara, María Jesús; Miras Portugal, María Teresa; Gómez Villafuertes, María Rosa; Carracedo Rodríguez, Juan Gonzalo; Pintor, JesúsPurpose: The objective of this study was to evaluate the changes in the melatoninergic receptors of DBA/2J and C57BL/6J mice with the development of glaucoma. DBA/2J mice are widely used to study the physiopathology of glaucoma due to the similarities of their eyes to human eyes and the resulting similarity in the development of their pathology. In addition, melatoninergic receptors are known for their control of intraocular pressure (IOP), reducing the production of aqueous humor; however, little is known about their relationship with the development of this pathology. Methods: mRNA expression of MT1, MT2, and GPR50 melatoninergic receptors was performed with quantitative real-time PCR. In addition, receptor expression was performed with immunohistochemical techniques on the ciliary processes. To further investigate the effect of melatonin and its analog 5-methoxycarbonylamino-N-acetyltryptamine (5-MCA-NAT) on IOP, animals were instilled with these compounds and the corresponding melatoninergic antagonists to assess their effect on IOP. Results: All melatoninergic receptor expression decayed with the development of the glaucomatous pathology in the DBA/2J mice, and was especially visible for the MT2 receptor. However, receptor expression was consistent in the C57BL/6J control mice across all ages investigated. Furthermore, IOP blockage was stronger with 4PPDOT (MT2 antagonist) only in the DBA/2J mice which suggests a correlation of this receptor with the development of the glaucomatous pathology in DBA/2J animals. Conclusions: Melatonin receptor expression decays with the development of the glaucomatous pathology. This implies that the physiologic hypotensive effect of endogenous melatonin reducing IOP is not possible. A solution for such changes in receptor expression is the exogenous application of melatonin or any of its analogs that permit the activation of the remaining melatonin receptors.
- PublicationChanges in P2Y Purinergic Receptor Expression in the Ciliary Body in a Murine Model of Glaucoma(Frontiers Media, 2017-10-10) Fonseca Vázquez, Begoña; Martínez Águila, Alejandro; Pérez de Lara, María Jesús; Miras Portugal, María Teresa; Gómez Villafuertes, María Rosa; Pintor, JesúsGlaucoma is a neuropathology, often accompanied by an elevated intraocular pressure (IOP), which can lead to blindness. Since DBA/2J mice develop glaucoma, several studies of the physiopathology of glaucoma have been reported in this animal model. It is also known that purinergic receptors are involved in the pathology of glaucoma by controlling aqueous humor production and drainage and therefore controlling IOP. There are no studies on purinergic receptors in the DBA/2J model of glaucoma and their relation to the development of the pathology, so the aim of this study was to make an approach to the purinergic mechanisms involved in glaucoma. All the experiments were performed using DBA/2J and C57BL/6J mice and investigating P2Y1, P2Y2, and P2Y6 receptors. IOP measurements were made with a non-invasive rebound tonometer, and animals were instilled with diadenosine tetraphosphate (Ap4A) and the corresponding purinergic antagonists in order to see their effects on IOP. The expression of mRNA for P2Y1, P2Y2, and P2Y6 purinergic receptors was carried out by quantitative real-time PCR. Additionally, P2Y-receptor expression was performed by immunohistochemical techniques carried out on the ciliary processes. The results showed that IOP decreases when Ap4A was instilled and that the expressions of the analyzed purinergic receptors were stable throughout all the ages under study in the C57BL/6J mice (control mice). On the other hand, there were significant changes in the purinergic receptor expression in DBA/2J suggesting that elevated IOP in these animals could be related to an increase of P2Y2 expression and a decrease in P2Y1 receptors.
- PublicationAprendizaje basado en proyectos y aula invertida de la asignatura de Bioquímica del grado de Veterinaria: elaboración de nuevo material docente y empleo de la red social YouTube como vía de divulgación y evaluación(2021-06) Díaz Hernández, Miguel; Gómez Villafuertes, María Rosa; Gualix Sánchez, Francisco Javier; Martín Herranz, Ricardo; Ortega de la O, Felipe; Torres Molina, Magdalena IsabelEn el Grado de Veterinaria, la asignatura de Bioquímica tiene un carácter troncal pero es percibida por el alumnado como distante respecto a los contenidos generales del resto de la titulación. Por tanto, este proyecto surge para motivar a los alumnos de primero de Veterinaria a que aprendan a estudiar y aprender Bioquímica, una asignatura básica y fundamental pero que para la mayoría de l@s alumn@s no es atractiva.En la mayoría de los casos, no abordan el estudio de la asignatura de la manera correcta, intentan memorizar, sin entender lo que están estudiando, en poco tiempo y así es imposible aprender. Se han utilizado diferentes herramientas pedagógicas (como: aprender a través de la resolución de casos y problemas y aula invertida) así como distintas herramientas digitales para poder llevarlo a cabo. Evidentemente, hemos necesitamos la colaboración voluntaria de l@s alumn@s. La participación en este proyecto ha sido voluntaria y se han seleccionado de forma aleatoria. Los alumnos han trabajado en parejas en la resolución de un caso, han elaborado una presentación y los han explicado a sus compañeros.
- PublicationIncreased Ap4A levels and ecto-nucleotidase activity in glaucomatous mice retina(Springer, 2018-06-08) Pérez de Lara, María Jesús; Guzmán Aránguez, Ana Isabel; Gómez Villafuertes, María Rosa; Gualix, Javier; Miras Portugal, María Teresa; Pintor, JesúsThe pathogenesis of glaucoma involves numerous intracellular mechanisms including the purinergic system contribution. Furthermore, the presence and release of nucleotides and dinucleotides during the glaucomatous damage and the maintenance of degradation machinery through ecto-nucleotidase activity are participating in the modulation of the suitable extracellular complex balance. The aim of this study was to investigate the levels of diadenosine tetraphosphate (Ap4A) and the pattern of ecto-nucleotidase activity expression in glaucomatous retinas during the progress the pathology. Ap4A levels were analyzed by HPLC in glaucomatous retinas from the DBA/2J mice at 3, 9, 15, and 23 months of age. For that, retinas were dissected as flattened whole-mounts and stimulated in Ringer buffer with or without 59 mM KCl. NPP1 expression was analyzed by RT-PCR and western blot and its distribution was assessed by immunohistochemistry studies examined under confocal microscopy. Glaucomatous mice exhibited Ap4A values, which changed in stimulated retinas as long as the pathology progressed varying from 0.73 ± 0.04 (3 months) to 0.170 ± 0.05 pmol/mg retina (23 months). Concomitantly, NPP1 expression was significantly increased (82.15%) in the DBA/2J mice at 15 months. Furthermore, immunohistochemical studies showed that NPP1 labeling was stronger in OPL and IPL labeling tangentially in the vitreal part of the retina and was upregulated at 15 months of age. Our findings demonstrate that Ap4A decreased levels may be related with exacerbated activity of NPP1 protein in glaucomatous degeneration and in this way contributing to elucidate different mechanisms involved in retinal impairment in glaucomatous degeneration.