Person:
Patiño Álvarez, Aurora Belén

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First Name
Aurora Belén
Last Name
Patiño Álvarez
URI
https://hdl.handle.net/20.500.14352/74371
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Genética, Fisiología y Microbiología
Area
Microbiología
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UCM identifierORCIDScopus Author IDWeb of Science ResearcherIDDialnet IDGoogle Scholar ID

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Now showing 1 - 10 of 13
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    Targeting conserved genes in fusarium species
    (Mycotoxigenic Fungi: Methods and Protocols, 2017) Gil-Serna, Jessica; Patiño Álvarez, Aurora Belén; Jurado, Miguel; Mirete Castañeda, Salvador; Vázquez Estévez, Covadoga; González Jaén, María Teresa
    Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions.
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    Ecophysiological characterization of Penicillium expansum population in lleida (Spain)
    (2008) Morales, Hector; Marín, Sonia; Obea, Laura; Domenech Lucas, Miriam; Patiño Álvarez, Aurora Belén; Ramos, Antonio J.; Sanchis, Vicente
    Penicillium expansum, a patulin producer fungus, is the most important fungus causing decay in cold stored both apples and pears. This can lead to patulin contaminated by-products. The aim of this assay was to evaluate the phenotypical and physiological variability in the population of P. expansum that cause fruit spoilage in post-harvest stages in Lleida (Spain). In total, 101 isolates of P. expansum from the 2004 and the 2005 seasons were obtained from decayed fruits. Significant differences were found in the observations from both seasons. Variability of the isolates in each season seemed to be partially explained by differences in growth in media, patulin accumulation and resistance to fungicides. Patulin production was detected in almost 100% of the isolates. Variability existing in P. expansum population could not be totally explained, but the above mentioned variables explained up to 74% of the diversity in some cases. The results obtained point to the existence of different populations of P. expansum in each season and may explain the differences in fungicide resistance observed between both seasons. The capacity to colonize apple flesh and some variables involved in fruit colonization were not a source of variation neither in each season nor when both seasons were compared. As storage rooms are cleaned and disinfected each season, this suggests that each season, the populations in storage rooms develop only from strains capable to colonize apple flesh. This may lead to rapid sporulation and spreading of spores.
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    Polymerase chain reaction (PCR) identification of Penicillium brevicompactum, a grape contaminant and mycophenolic acid producer
    (Food Additives & Contaminants, 2007) Patiño Álvarez, Aurora Belén; Medina, Á.; Domenech Lucas, Miriam; González Jaén, María Teresa; Jiménez, M.; Vázquez, C.
    Penicillium brevicompactum is a ubiquitous fungal species that contaminates diverse substrates and commodities and produces an array of metabolites toxic to human and animals. The present work has obtained evidence, by liquid chromatography (LC)-ion-trap mass spectrometry, of the ability of P. brevicompactum strains isolated from grapes to produce mycophenolic acid, a potent immunosuppressor. In order to facilitate early diagnosis of this species on commodities for human and animal consumption, a rapid, sensitive and specific polymerase chain reaction (PCR) assay for P. brevicompactum was developed. The specific primers were designed based on the ITS1-5.8S-ITS2ITS (Internal Transcribed Spacers of rRNA genes) multicopy region. This method provides a useful aid to detect the presence of this fungal species in grapes and other commodities in order to prevent the toxins produced entering the food chain.
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    Reconsidering the Co-Occurrence of Aspergillus flavus in Spanish Vineyards and Aflatoxins in Grapes
    (Agriculture, 2023) Melguizo Ávila, Clara Del Carmen; Patiño Álvarez, Aurora Belén; Ramos, Antonio J.; Vázquez Estévez, María Covadonga Inmaculada; Gil Serna, Jessica
    Aspergillus flavus is a xerophilic fungus whose geographical distribution is expected to change due to the current climate change scenario. Grapes are one of the most important crops worldwide, and it is essential to evaluate the risk posed by their contamination with potential mycotoxigenic species. Recently, a few reports have described A. flavus as an emerging contaminant in vineyards, which has led to a discussion on the need to legislate aflatoxin contents in grapes. Using a specific PCR assay, the occurrence of A. flavus was demonstrated in 43 out of 61 grape samples collected from Spanish vineyards. Considering the high incidence observed, the risk of the grapes becoming contaminated with aflatoxin was subsequently evaluated. Aspergillus flavus isolates from grapes can grow in grape-based media under a variety of environmental conditions, but they were unable to produce either aflatoxin B1 (AFB1) or aflatoxin B2 (AFB2) even though their ability to produce these toxins was confirmed in a permissive medium (CYA). These results confirm that climate change is affecting the distribution of mycotoxigenic fungi, thereby increasing the occurrence of A. flavus in vineyards, although the risk of the grapes becoming contaminated with aflatoxin needs to be reconsidered.
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    Project number: 110
    La curación de contenidos científicos como estrategia didáctica y divulgativa en Microbiología y otras áreas afines
    (2020) Gil-Serna, Jéssica; Arregui García-Rovés, Lucía; Guillen Maestro, Alberto; Martín Cereceda, María Mercedes; Pérez Uz, María Blanca; Serrano Barrero, Susana Lourdes; Silóniz Jiménez, María Isabel de; Vázquez Estévez, María Covadonga Inmaculada; Patiño Álvarez, Aurora Belén
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    Project number: PIMCD69/23-24
    Esto va de Micro: 10 retos, 10 mujeres
    (2024) Gil Serna, Jessica; Arregui García-Roves, Lucía; Ayllón Santiago, Tania; Casado Almarcha, Ana María; Craqui Cordeo, María de la Vega; Domenech Lucas, Miriam; García Romero, Sofía Ismerai; Gómez Albarrán, Carolina; Gómez Rodríguez de Guzmán, Lucía; Lago Espartero, Darío; Lizarazu Cubero, Carolina; Martín Cereceda, María Mercedes; Melguizo Ávila, Clara Del Carmen; Patiño Álvarez, Aurora Belén; Pavesio Toledano, Manuel; Pérez Uz, María Blanca; Pulido Vadillo, Mario; Racovita, Georgiana Valentina; Rey Menacho, Rubén; Sánchez López-Varela, Marta; Tajahuerce Ángel, Isabel; Valderrama Conde, María José; del Valle Romera, Ana; Vázquez Estévez, María Covadonga Inmaculada; Williams, Richard Alexander John
    “Esto va de Micro” es un pódcast de microbiología en el cual estudiantes de la Universidad Complutense realizan entrevistas a personas expertas en temas de interés en el campo de la microbiología. En el curso 2023/2024 quisimos aprovechar esta plataforma y poner en marcha este proyecto de innovación docente para dar visibilidad al trabajo de microbiólogas relevantes y explicar a la sociedad el papel de la microbiología en la consecución de los Objetivos de Desarrollo Sostenible. El resultado han sido 11 episodios de pódcast divulgativos que están disponibles en las plataformas de iVoox y Spotify.
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    Project number: 136
    La Microbiología a debate
    (2021) Gil Serna, Jéssica; Arregui García-Rovés, Lucía; Belda Aguilar, Ignacio; Martín Cereceda, María Mercedes; Patiño Álvarez, Aurora Belén; Pérez Uz, María Blanca; Serrano Barrero, Susana Lourdes; Vázquez Estévez, María Covadonga
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    Project number: 136
    Esto va de Micro
    (2023) Gil Serna, Jéssica; Arregui García-Rovés, Lucía; Ayllón Santiago, Tania; Barbero Richart, Manuel Carlos; Cabello García, Sonia; Calvete del Olmo, Pablo; Casado Almarcha, Ana María; Doménech Lucas, Miriam; Echaide Torreguitar, Mercedes; García Pérez, Raquel; García de la Camacha Selgas, Nuria; Gómez Albarrán, Carolina; Herranz Clemente, Tania; Lago Espartero, Darío; Marín Pérez, María; Martín Cereceda, María Mercedes; Melguizo Ávila, Clara del Carmen; Patiño Álvarez, Aurora Belén; Pérez Uz, María Blanca; Pulido Vadillo, Mario; Romero Palomino, María Jesús; Valderrama Conde, María José; Vázquez Estévez, María Covadonga Inmaculada; Williams, Richard
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    MicroMundo@UCM: Ciencia ciudadana y aprendizaje-servicio frente a la resistencia a antibióticos desde la comunidad educativa
    (2023) Jiménez Cid, Víctor; Ayllón Santiago, Tania; Amaro Torres, Francisco; Calvo De Pablo, Pilar; Domenech Lucas, Miriam; De Francisco Martínez, Patricia; Patiño Álvarez, Aurora Belén; Vázquez Estévez, María Covadonga Inmaculada; Barbero Úriz, Óscar; Blesa Esteban, Alba Mercedes; Borrajo López, Ana; Cortés Prieto, Isabel; Fernández-Acero Bascones, Teresa; Fernández-Vega Granado, Alejandro; García Pastor, Lucía; González Rubio, Gema; Lavilla García, Beatriz; López Montesino, Sara; Martínez López, Raquel María; Molina Martín, María; Mascaraque Martín, Victoria; Parra Giraldo, Claudia Marcela; Rodríguez Escudero, María Isabel; Rodríguez Fernández, Carmina; Román González, Elvira; Rubio Lozano, Alba Victoria; Del Val Oriza, Elba; Valentí Sanguino, Marta; Bezos Garrido, Javier; Borrero Del Pino, Juan; Díaz Formoso, Lara; Escudero García-Calderón, José Antonio; García Benzaquén, Nerea; González Zorn, Bruno; Hipólito Carrillo de Albornoz, Alberto; Muñoz Atienza, Estefanía; Pérez Sancho, Marta; Pulido Vadillo, Mario; Romero Martínez, Beatriz; Sánchez Méndez, Irene; Serna Bernaldo, Carlos; Suárez Rodríguez, Mónica; Vergara González, Ester; Arias López, Patricia; González de Figueras, Carolina; Prieto Orzanco, Alicia; Sempere García, Julio; Gil Serna, Jessica; Maestro García-Donas, María Beatriz; García García, Aina; Gil Serna, Jessica
    Según ha establecido la OMS y la Asamblea de las Naciones Unidas, la resistencia a los antibióticos es una de las prioridades en Salud Global para el s. XXI, al mismo nivel que la preparación frente a pandemias emergentes y el desarrollo de vacunas y tratamientos frente a tuberculosis, malaria, HIV y otras enfermedades infecciosas. El abordaje de estos problemas sólo será eficaz desde la perspectiva multidisciplinar One Health (Un Mundo: Una Salud), que integra Salud Medioambiental, Animal y Humana. Desde 2016 la UCM ha sido pionera en implicar a las comunidades universitaria y preuniversitaria en la estrategia de aprendizaje activo mediante ApS “MicroMundo” (www.ucm.es/small-world-initiative), un proyecto multidisciplinar basado en ApS en el ámbito de la Biomedicina. Se trata de una adaptación al ApS de la estrategia internacional de studentsourcing Tiny Earth (www.tinyearthnet.org). MicroMundo implica a estudiantes de ESO y Bachillerato a participar como investigadores en un proyecto real para el descubrimiento de nuevos antibióticos a partir de muestras de suelos. El objetivo final del servicio es acercar la cultura científica, la perspectiva One Health y la investigación biomédica a la sociedad. Para ello, colaboramos con una red de colegios e institutos de la Comunidad de Madrid. Un objetivo adicional es fomentar la vocación por Grados STEM y por la I+D. Para lograr estos objetivos, los estudiantes universitarios, objeto del aprendizaje, se responsabilizan de facilitar a los jóvenes estudiantes el entrenamiento y material necesarios para llevar a cabo los experimentos, así como dirigir el trabajo de investigación y diversas estrategias de divulgación hacia la comunidad. El éxito del proyecto en la UCM ha inspirado su implementación en más de 30 universidades en España y Portugal y ha merecido el Premio PRAN 2021 a la comunicación y sensibilización de la población sobre la resistencia a los antibióticos. En esta publicación presentamos la memoria final del proyecto MicroMundo@UCM realizado durante el curso 2022-23 en 33 centros educativos de la Comunidad de Madrid.
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    @mycoorg, trabajando para conseguir alimentos libres de micotoxinas
    (SEM@foro, 2023) Gil Serna, Jessica; Vázquez Estévez, María Covadonga Inmaculada; Patiño Álvarez, Aurora Belén