Person:
Patiño Álvarez, Aurora Belén

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First Name
Aurora Belén
Last Name
Patiño Álvarez
URI
https://hdl.handle.net/20.500.14352/74371
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Genética, Fisiología y Microbiología
Area
Microbiología
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Author: Jiménez, M. × End date: 2009 ×

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    Polymerase chain reaction (PCR) identification of Penicillium brevicompactum, a grape contaminant and mycophenolic acid producer
    (Food Additives & Contaminants, 2007) Patiño Álvarez, Aurora Belén; Medina, Á.; Domenech Lucas, Miriam; González Jaén, María Teresa; Jiménez, M.; Vázquez, C.
    Penicillium brevicompactum is a ubiquitous fungal species that contaminates diverse substrates and commodities and produces an array of metabolites toxic to human and animals. The present work has obtained evidence, by liquid chromatography (LC)-ion-trap mass spectrometry, of the ability of P. brevicompactum strains isolated from grapes to produce mycophenolic acid, a potent immunosuppressor. In order to facilitate early diagnosis of this species on commodities for human and animal consumption, a rapid, sensitive and specific polymerase chain reaction (PCR) assay for P. brevicompactum was developed. The specific primers were designed based on the ITS1-5.8S-ITS2ITS (Internal Transcribed Spacers of rRNA genes) multicopy region. This method provides a useful aid to detect the presence of this fungal species in grapes and other commodities in order to prevent the toxins produced entering the food chain.