Polymerase chain reaction (PCR) identification of Penicillium brevicompactum, a grape contaminant and mycophenolic acid producer

Patiño Álvarez, Aurora Belén; Medina, Á.; Domenech Lucas, Miriam; González Jaén, María Teresa; Jiménez, M.; Vázquez, C.

Polymerase chain reaction (PCR) identification of Penicillium brevicompactum, a grape contaminant and mycophenolic acid producer

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Patiño Food Additives and Contaminants 2007.pdf (130.02 KB)

Official URL

https://www.tandfonline.com/doi/full/10.1080/02652030600967222

Publication date

2007

Authors

Patiño Álvarez, Aurora Belén

Medina, Á.

Domenech Lucas, Miriam

González Jaén, María Teresa

Jiménez, M.

Vázquez, C.

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URI

https://hdl.handle.net/20.500.14352/98309

Abstract

Penicillium brevicompactum is a ubiquitous fungal species that contaminates diverse substrates and commodities and produces an array of metabolites toxic to human and animals. The present work has obtained evidence, by liquid chromatography (LC)-ion-trap mass spectrometry, of the ability of P. brevicompactum strains isolated from grapes to produce mycophenolic acid, a potent immunosuppressor. In order to facilitate early diagnosis of this species on commodities for human and animal consumption, a rapid, sensitive and specific polymerase chain reaction (PCR) assay for P. brevicompactum was developed. The specific primers were designed based on the ITS1-5.8S-ITS2ITS (Internal Transcribed Spacers of rRNA genes) multicopy region. This method provides a useful aid to detect the presence of this fungal species in grapes and other commodities in order to prevent the toxins produced entering the food chain.