Polymerase chain reaction (PCR) identification of Penicillium brevicompactum, a grape contaminant and mycophenolic acid producer

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dc.contributor.authorPatiño Álvarez, Aurora Belén
dc.contributor.authorMedina, Á.
dc.contributor.authorDomenech Lucas, Miriam
dc.contributor.authorGonzález Jaén, María Teresa
dc.contributor.authorJiménez, M.
dc.contributor.authorVázquez, C.
dc.date.accessioned2024-02-02T13:10:36Z
dc.date.available2024-02-02T13:10:36Z
dc.date.issued2007-02
dc.description.abstractPenicillium brevicompactum is a ubiquitous fungal species that contaminates diverse substrates and commodities and produces an array of metabolites toxic to human and animals. The present work has obtained evidence, by liquid chromatography (LC)-ion-trap mass spectrometry, of the ability of P. brevicompactum strains isolated from grapes to produce mycophenolic acid, a potent immunosuppressor. In order to facilitate early diagnosis of this species on commodities for human and animal consumption, a rapid, sensitive and specific polymerase chain reaction (PCR) assay for P. brevicompactum was developed. The specific primers were designed based on the ITS1-5.8S-ITS2ITS (Internal Transcribed Spacers of rRNA genes) multicopy region. This method provides a useful aid to detect the presence of this fungal species in grapes and other commodities in order to prevent the toxins produced entering the food chain.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Educación y Ciencia
dc.description.sponsorshipGobierno de Valencia
dc.description.statuspub
dc.identifier.doi10.1080/02652030600967222
dc.identifier.essn1464–5122
dc.identifier.issn0265-203X
dc.identifier.officialurlhttps://www.tandfonline.com/doi/full/10.1080/02652030600967222
dc.identifier.urihttps://hdl.handle.net/20.500.14352/98309
dc.issue.number2
dc.journal.titleFood Additives & Contaminants
dc.language.isoeng
dc.page.final172
dc.page.initial165
dc.relation.projectID(Project Nos AGL-2001-2974-C05-01 and AGL-2004-07549-C05-02/ALI)
dc.relation.projectIDProject No. GV04B-111
dc.rights.accessRightsrestricted access
dc.subject.cdu579
dc.subject.cdu663.1
dc.subject.keywordPenicillium brevicompactum
dc.subject.keywordMycophenolic acid
dc.subject.keywordPolymerase chain reaction (PCR)
dc.subject.keywordGrapes
dc.subject.keywordLiquid chromatography (LC)-ion-trap mass spectrometry
dc.subject.ucmMicrobiología (Biología)
dc.subject.ucmTecnología de los alimentos
dc.subject.unesco3309.90 Microbiología de Alimentos
dc.titlePolymerase chain reaction (PCR) identification of Penicillium brevicompactum, a grape contaminant and mycophenolic acid producer
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number24
dspace.entity.typePublication
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relation.isAuthorOfPublicationdcd2e345-2d1a-4f7b-a550-13c6ca128709
relation.isAuthorOfPublicationc6e49c5c-cd3d-4aa3-b582-4a5221825dc0
relation.isAuthorOfPublication.latestForDiscoveryb71ac7e7-f329-4d04-b48d-8b518b822d0b
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