Person: Agüí Chicharro, María Lourdes
Faculty / Institute
Implementación de la metodología flipped classroom en los laboratorios de Química Analítica
2023-05-31, Reviejo García, Ángel Julio, Agüí Chicharro, María Lourdes, Campuzano Ruiz, Susana, Gamella Carballo, Maria, García Martín, Ángel Felipe, González Cortés, Araceli, Guerrero Blanco, José Ignacio, Mateos Briz, María Raquel, Miguel Bravo, María, Pérez Ginés, Víctor, Reviejo Martínez, Eva, Romano Martín, Santiago, Ruiz-Valdepeñas Montiel, Víctor, Sánchez Tirado, Esther, Santiago Sáez, Andrés Sebastián, Serafín González-Carrato, Verónica, Torrente Rodríguez, Rebeca Magnolia, Yáñez-Sedeño, Paloma, Pedrero Muñoz, María
Adaptar el sistema tradicional de aprendizaje a las necesidades actuales del alumnado empleando la metodología flipped classroom en el laboratorio de Química Analítica I, con el objetivo de fomentar el aprendizaje utilizando herramientas digitales.
What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications
2019-10-29, Yáñez Sedeño, Paloma, Agüí Chicharro, María Lourdes, Campuzano Ruiz, Susana, Pingarrón Carrazón, José Manuel
This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.
Electrochemical (Bio)Sensing Devices for Human-Microbiome-Related Biomarkers
2023, Sánchez-Tirado, Esther, Agüí Chicharro, María Lourdes, González-Cortés, Araceli, Campuzano Ruiz, Susana, Yáñez-Sedeño, Paloma, Pingarrón Carrazón, José Manuel
The study of the human microbiome is a multidisciplinary area ranging from the field of technology to that of personalized medicine. The possibility of using microbiota biomarkers to improve the diagnosis and monitoring of diseases (e.g., cancer), health conditions (e.g., obesity) or relevant processes (e.g., aging) has raised great expectations, also in the field of bioelectroanalytical chemistry. The well-known advantages of electrochemical biosensors—high sensitivity, fast response, and the possibility of miniaturization, together with the potential for new nanomaterials to improve their design and performance—position them as unique tools to provide a better understanding of the entities of the human microbiome and raise the prospect of huge and important developments in the coming years. This review article compiles recent applications of electrochemical (bio)sensors for monitoring microbial metabolites and disease biomarkers related to different types of human microbiome, with a special focus on the gastrointestinal microbiome. Examples of electrochemical devices applied to real samples are critically discussed, as well as challenges to be faced and where future developments are expected to go..
An Electrochemical Enzyme Biosensor for 3-Hydroxybutyrate Detection Using Screen-Printed Electrodes Modified by Reduced Graphene Oxide and Thionine
2017-11-11, Martínez García, Gonzalo, Pérez Julián, Elena, Agüí Chicharro, María Lourdes, Cabré, Naomí, Joven, Jorge, Yáñez Sedeño, Paloma, Pingarrón Carrazón, José Manuel
A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate or the sample in the presence of NAD+ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0.010 and 0.400 mM 3-HB which covers the clinically relevant levels for diluted serum samples. In addition, a limit of detection of 1.0 µM, much lower than that reported using other biosensors, was achieved. The analytical usefulness of the developed biosensor was demonstrated via application to spiked serum samples.