Person: Bonnin Arias, Cristina Natalia
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First Name
Cristina Natalia
Last Name
Bonnin Arias
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
Identifiers
18 results
Search Results
Now showing 1 - 10 of 18
Item Dispositivo y Método simulador de entornos para la evaluación de la función visual(2018) Sánchez Ramos, Celia; Bonnin Arias, Cristina Natalia; Universidad Complutense de MadridDispositivo y método simulador de entornos de evaluación de la función visual que comprende un soporte que aloja un luxómetro medidor de la iluminación ambiente que comprende una pluralidad de dioptrios de densidad óptica neutra (DO); y donde la relación entre la densidad óptica y la transmitancia (T) del dioptrio viene dada por DO=log(1/T); y donde los dioptrios de densidad óptica neutra (DO) están dispuestos en el soporte en correspondencia con el sistema visual de un individuo y configurados para alcanzar distintos niveles de iluminación directamente en el sistema visual del individuo sin realizar una variación real de la iluminación ambiente.Item Effects of Light‐emitting Diode Radiations on Human Retinal Pigment Epithelial Cells in vitro(Photochemistry and Photobiology, 2012) Chamorro, Eva; Muñoz de Luna, Javier; Bonnin Arias, Cristina Natalia; Pérez Carrasco, María Jesús; Vázquez Molini, Daniel; Sánchez Ramos, CeliaHuman visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.Item Light regulates the expression of the BDNF/TrkB system in the adult Zebrafish retina(Microscopy Research and Technique, 2012) Guerrera, María Cristina; García Calavia, Marta; Chamorro Gutiérrez, Eva; Montalbano, Giuseppe; López Velasco, Salvador; López Muñiz, Alfonso Joaquín; Germanà, Antonino; Vega Álvarez, José Antonio; Sánchez Ramos, Celia; Bonnin Arias, Cristina NataliaThe retina of the adult zebrafish express brain‐derived neurotrophic factor (BDNF) and its signaling receptor TrkB. This functional system is involved in the biology of the vertebrate retina and its expression is regulated by light. This study was designed to investigate the effects of cyclic (12 h light/12 h darkness) or continuous (24 h) exposure during 10 days to white light, white‐blue light, and blue light, as well as of darkness, on the expression of BDNF and TrkB in the retina. BDNF and TrkB were assessed in the retina of adult zebrafish using quantitative real‐time polymerase chain reaction and immunohistochemistry. Exposure to white, white‐blue, and blue light causes a decrease of BDNF mRNA and of BDNF immunostaining, independently of the pattern of light exposition. Conversely, in the same experimental conditions, the expression of TrkB mRNA was upregulated and TrkB immunostaining increased. Exposition to darkness diminished BDNF and TrkB mRNAs, and abolished the immunostaining for BDNF but not modified that for TrkB. These results demonstrate the regulation of BDNF and TrkB by light in the retina of adult zebrafish and might contribute to explain some aspects of the complex pathophysiology of light‐induced retinopathies.- Project number: 286
Item Atlas electrónico de registros de retinográficos y tomográficos: cribado, derivación, y modelización matématica. Parte III: Retinopatía Diabética(2017) Sánchez Ramos, Celia; Pérez Carrasco, María Jesús; Bonnin Arias, Cristina Natalia; Llorens Casado, María Belén; Panetsos Petrova, FivosEl objetivo de este proyecto es continuar con el Atlas retinográfico iniciado en las convocatorias anteriores con imágenes obtenidas con los principales y novedosos instrumentos utilizados en la actualidad para el diagnóstico de patologías retinianas. El Atlas presentará, además de las imágenes, una detallada descripción de los aspectos que debe tenerse en cuenta en el momento de realizar el cribado. Y además, se realizará un novedoso modelizado matemático de las imágenes. Esta tercera parte se centrará en el diagnóstico de RETINOPATÍAS DIABÉTICAS, una patología con una prevalencia del 4% de la población en general y que constituye una de las primeras causas de ceguera no reversible en los países desarrollados, causando incapacidad laboral permanente. El Atlas puede ser de gran interés para los enfermos de DIABETES ya que está probado que el 100% de los afectados a los 15 años padecen RETINOPATÍAS DIABÉTICAS. Por lo que conviene que sepan el estado del fondo de su ojo. Estará constituida por una extensa relación de retinografías (fotografías del fondo de ojo – retina) y tomografías de coherencia óptica (imágenes de cortes histológicos de la retina en vivo), exponiendo casos reales de patologías comunes y poco comunes. Constituye una herramienta con triple función: por un lado será un elemento didáctico para el aprendizaje de patologías retinianas y el método de diagnóstico por imagen; en segundo lugar, podrá utilizarse para realizar evaluaciones tipo test; y, en tercer lugar, constituirá una completa base de datos que podrá ser utilizado como material de consulta y diagnóstico comparado en la práctica clínica. En definitiva, se trata de una herramienta muy económica de gran utilidad para un amplio grupo de profesionales sanitarios expertos y especialistas en visión y en sistema ocular. - Project number: 163
Item Atlas electrónico de registros retinográficos y tomográficos: cribado, derivación, diagnóstico diferencial y seguimiento de afecciones retinianas Parte IV: Retinopatía del Prematuro(2018) Sánchez Ramos, Celia; Pérez Carrasco, María Jesús; Bonnin Arias, Cristina Natalia; Panetsos Petrova, Fivos; Blanco Torcal, Barbara Prisca; Navarro Blanco, Carolina; Blázquez Sánchez, VanesaEl objetivo de este proyecto es continuar con el Atlas retinográfico iniciado en las convocatorias anteriores con imágenes obtenidas con los principales y novedosos instrumentos utilizados en la actualidad para el diagnóstico de patologías retinianas. El Atlas presenta, además de las imágenes, una detallada descripción de los criterios cribado. Esta cuarta parte se centra en el diagnóstico por imagen de la retinopatía del prematuro, que se define como una vitreorretinopatía fibro y vasoproliferativa periférica que acontece en los recién nacidos inmaduros, generalmente sometidos a oxigenoterapia. El Atlas está constituido por una extensa relación de retinografías (fotografías del fondo de ojo–retina) y tomografías de coherencia óptica (imágenes de cortes histológicos de la retina en vivo), exponiendo casos reales de patologías comunes y poco comunes. Constituye una herramienta con triple función: por un lado, será un elemento didáctico para el aprendizaje de patologías retinianas y el método de diagnóstico por imagen; en segundo lugar, puede utilizarse para realizar autoevaluaciones y, en tercer lugar, constituye una completa base de datos de casos clínicos. En definitiva, se trata de una herramienta muy económica y de gran utilidad para un amplio grupo de profesionales sanitarios expertos y especialistas en sistema visual. Item New Optical Filter Plate for Use as Eye Protection by Welders(Welding journal, 2013) Langa Moraga, Antonio; Bonnin Arias, Cristina Natalia; Chamorro, Eva; Pérez Carrasco, María Jesús; Sánchez Ramos, CeliaPeople whose work tasks involve the use of welding torches are at special risk of suffering eye injuries due to the emission of visible, short-wavelength radiation. Current legislation requires that a company provide its employees with protection against the harmful radiation produced by welding equipment. Often, however, a worker will be reluctant to use protective goggles since these markedly cut visibility and can consequently lead to errors or even burns. This practice of avoiding the use of protection makes them susceptible to suffer irreversible severe retinal damage leading to partial or complete loss of vision. In this paper, we propose the use of a new photoprotective filter in the form of safety goggles that seeks to improve the compromised vision produced by conventional filters. We compare a series of visual function variables in 36 adults, aged 30 to 58 years, using the new optical filter and a conventional filter used for welding protection. Our findings suggest that the filter proposed provides optimal protection against the harmful effects of short-wavelength radiation while minimizing the reduced vision effects of conventional filters used for this purpose.Item Expression of TRPV4 in the zebrafish retina during development(Microscopy Research and Technique, 2012) Guerrera, María Cristina; García Calavia, Marta; Laurà, Rosaria; Germanà, Antonino; Vega Álvarez, José Antonio; Sánchez Ramos, Celia; Bonnin Arias, Cristina NataliaThe transient receptor potential (TRP) channels are involved in sensing mechanical/physical stimuli such as temperature, light, pressure, as well as chemical stimuli. Some TRP channels are present in the vertebrate retina, and the occurrence of the multifunctional channel TRP vanilloid 4 (TRPV4) has been reported in adult zebrafish. Here, we investigate the expression and distribution of TRPV4 in the retina of zebrafish during development using polymerase chain reaction (PCR), Western blot, and immunohistochemistry from 3 days post fertilization (dpf) until 100 dpf. TRPV4 was detected at the mRNA and protein levels in the eye of zebrafish at all ages sampled. Immunohistochemistry revealed the presence of TRPV4 in a population of the retinal cells identified as amacrine cells on the basis of their morphology and localization within the retina, as well as the co‐localization of TRPV4 with calretinin. TRPV4 was first (3 dpf) found in the soma of cells localized in the inner nuclear and ganglion cell layers, and thereafter (10 dpf) also in the inner plexiform layer. The adult pattern of TRPV4 expression was achieved by 40 dpf the expression being restricted to the soma of some cells in the inner nuclear layer and ganglion cell layers. These data demonstrate the occurrence and developmental changes in the expression and localization of TRPV4 in the retina of zebrafish, and suggest a role of TRPV4 in the visual processing.Item Utilidad de la pupilometría dinámica en el control de alcoholemia de los conductores(Adicciones, 2013) Lobato Rincón, Luis-Lucio; Cabanillas Campos, María del Carmen; Navarro Valls, Juan José; Bonnin Arias, Cristina Natalia; Chamorro Gutiérrez, Eva; Sánchez Ramos, CeliaLa pupilometría se ha convertido en una herramienta de gran interés en las Ciencias de la Visión. Hasta el momento, solo unos pocos estudios han explorado la relación entre el reflejo pupilar y el consumo de drogas. El objetivo de este estudio fue evaluar la aplicabilidad de la pupilometría dinámica mesópica como método objetivo para la detección de niveles de alcoholemia por encima del límite legal. Se utilizó un diseño de carácter cuasi-experimental, con 19 voluntarios participando en dos condiciones (pre y post ingesta de alcohol). En la sesión con alcohol, los participantes consumieron 27.5 ml de alcohol en intervalos de 60 minutos durante 4 horas. Se llevaron a cabo registros de pupilometría mediante el Power Refractor II, utilizándose cuatro tipos de estimulación lumínica: blanca (5600 K), azul (450 nm), verde (510 nm) y roja (600 nm). El diámetro basal aumentó significativamente a partir de concentraciones de alcohol en aire espirado iguales o mayores de 0.25 mg/l. Además, el valor de la amplitud de constricción para la luz roja también ofreció diferencias significativas entre las dos condiciones. Estos resultados resultan prometedores en la búsqueda de nuevos métodos de detección de niveles de alcoholemia no permitidos al volante. En este estudio se demuestra que el incremento del diámetro basal y la amplitud pupilar pueden ser utilizados como indicador del nivel de consumo de alcohol. Son necesarios más estudios para validar éste y otros métodos de diagnóstico complementarios a las actuales pruebas de alcoholemia y de consumo de sustancias psicotropas.Item Pupillary behavior in relation to wavelength and age(Frontiers in Human Neuroscience, 2014) Lobato Rincón, Luis-Lucio; Cabanillas Campos, María del Carmen; Bonnin Arias, Cristina Natalia; Chamorro Gutiérrez, Eva; Sánchez Ramos, Celia; Murciano Cespedosa, AntonioPupil light reflex can be used as a non-invasive ocular predictor of cephalic autonomic nervous system integrity. Spectral sensitivity of the pupil's response to light has, for some time, been an interesting issue. It has generally, however, only been investigated with the use of white light and studies with monochromatic wavelengths are scarce. This study investigates the effects of wavelength and age within three parameters of the pupil light reflex (amplitude of response, latency, and velocity of constriction) in a large sample of younger and older adults (N = 97), in mesopic conditions. Subjects were exposed to a single light stimulus at four different wavelengths: white (5600° K), blue (450 nm), green (510 nm), and red (600 nm). Data was analyzed appropriately, and, when applicable, using the General Linear Model (GLM), Randomized Complete Block Design (RCBD), Student's t-test and/or ANCOVA. Across all subjects, pupillary response to light had the greatest amplitude and shortest latency in white and green light conditions. In regards to age, older subjects (46-78 years) showed an increased latency in white light and decreased velocity of constriction in green light compared to younger subjects (18-45 years old). This study provides data patterns on parameters of wavelength-dependent pupil reflexes to light in adults and it contributes to the large body of pupillometric research. It is hoped that this study will add to the overall evaluation of cephalic autonomic nervous system integrity.Item Visual Discrimination Increase by Yellow Filters in Retinitis Pigmentosa(Optometry and Vision Science, 2016) Cedrún Sánchez, Juan Enrique; Chamorro Gutiérrez, Eva; Bonnin Arias, Cristina Natalia; Aguirre Vilacoro, Victoria; Castro, José J.; Sánchez Ramos, CeliaPURPOSE: The objective of this study was to evaluate, by halometry and under low illumination conditions, the effects of short-wavelength light absorbance filters on visual discrimination capacity in retinitis pigmentosa patients. METHODS: This was an observational, prospective, analytic, and transversal study on 109 eyes of 57 retinitis pigmentosa patients with visual acuity better than 1.25 logMAR. Visual disturbance index (VDI) was determined using the software Halo 1.0, with and without the interposition of filters which absorb (totally or partially) short-wavelength light between 380 and 500 nm. RESULTS: A statistically significant reduction in the VDI values determined using filters which absorb short-wavelength light was observed (p < 0.0001). The established VDIs in patients with VA logMAR <0.4 were 0.30 ± 0.05 (95% CI, 0.26–0.36) for the lens alone, 0.20 ± 0.04 (95% CI, 0.16–0.24) with the filter that completely absorbs wavelengths shorter than 450 nm, and 0.24 ± 0.04 (95% CI, 0.20–0.28) with the filter that partially absorbs wavelengths shorter than 450 nm, which implies a 20 to 33% visual discrimination capacity increase. In addition, a decrease of VDI in at least one eye was observed in more than 90% of patients when using a filter. CONCLUSIONS: Short-wavelength light absorbance filters increase visual discrimination capacity under low illumination conditions in retinitis pigmentosa patients. Use of such filters constitutes a suitable method to improve visual quality related to intraocular light visual disturbances under low illumination conditions in this group of patients. © 2016 American Academy of Optometry