Person:
Bonnin Arias, Cristina Natalia

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First Name
Cristina Natalia
Last Name
Bonnin Arias
URI
https://hdl.handle.net/20.500.14352/79903
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
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2012 - 20182
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Start date: 2012 × Subject: 628.9 ×

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    Effects of Light‐emitting Diode Radiations on Human Retinal Pigment Epithelial Cells in vitro
    (Photochemistry and Photobiology, 2012) Chamorro, Eva; Muñoz de Luna, Javier; Bonnin Arias, Cristina Natalia; Pérez Carrasco, María Jesús; Vázquez Molini, Daniel; Sánchez Ramos, Celia
    Human visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.
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    Diferentes fuentes de luz artificial: Comparación de radiación ultravioleta y luz azul.
    (Franja visual, 2018) Bonnin Arias, Cristina Natalia; Gutiérrez Jorrín, Sara.; Rodríguez Alonso, Xabier; Sánchez Ramos, Celia
    La radiación emitida por fuentes artificiales se compone de luz visible, de radiación ultravioleta e infrarroja en proporciones diferentes a las presentes en la radiación solar, a pesar de que se pretende replicar a la luz del sol. La composición de la luz emitida por estas fuentes genera preoupación con relación a sus efectos sobre distintos aspectos del organismo, entre los que destaca la visión. La radiación UV y la banda azul de la luz son potencialmente más dañinas, dado que poseen una mayor energía: concretamente, según la Asociación Americana de Oftalmología en su informe de 2008, la radiación de 435nm proporciona 10 veces más energía que un haz de luz de 500nm, incluso dentro del espectro uv, la raciación de 270nm es 1000 veces más energética que el UVA de 320 nm,