Person: Sevane Fernández, Natalia
Universidad Complutense de Madrid
Faculty / Institute
Now showing 1 - 3 of 3
- PublicationAge Effects Aggressive Behavior: RNA-Seq Analysis in Cattle with Implications for Studying Neoteny Under Domestication(Springer Nature, 2022-01-15) García Eusebi, Paulina; Sevane Fernández, Natalia; O’Rourke, Thomas; Pizarro Díaz, Manuel; Boeckx, Cedric; Dunner Boxberger, SusanaThe reactive type of aggression is regulated mostly by the brain’s prefrontal cortex; however, the molecular changes underlying aggressiveness in adults have not been fully characterized. We used an RNA-seq approach to investigate diferential gene expression in the prefrontal cortex of bovines from the aggressive Lidia breed at diferent ages: young three-year old and adult four-year-old bulls. A total of 50 up and 193 down-regulated genes in the adult group were identifed. Furthermore, a cross-species comparative analysis retrieved 29 genes in common with previous studies on aggressive behaviors, representing an above-chance overlap with the diferentially expressed genes in adult bulls. We detected changes in the regulation of networks such as synaptogenesis, involved in maintenance and refnement of synapses, and the glutamate receptor pathway, which acts as excitatory driver in aggressive responses. The reduced reactive aggression typical of domestication has been proposed to form part of a retention of juvenile traits as adults (neoteny).
- PublicationA novel missense variant in endothelin‐2 ( EDN2 ) causes a growth and respiratory lethal syndrome in bovine(Wiley, 2022-07-20) Eusebi, Paulina G.; Cortés Gardyn, Óscar; Contreras, Elisabeth; Cañón Ferreras, Javier; Dunner Boxberger, Susana; Sevane Fernández, NataliaThe high level of fragmentation of the Spanish Lidia cattle breed, divided into lineages called ‘castas’ and into herds within lineages based on reproductive isolation, increases the risk of homozygosity and the outbreak of recessive genetic defects. Since 2004, an increasing number of calves have been identified in a Lidia herd with signs of severe growth retardation, respiratory alterations and juvenile lethality, which constitutes a novel inherited syndrome in cattle and was subsequently termed growth and respiratory lethal syndrome. We performed a genome-wide association study on a cohort of 13 affected calves and 24 putative non-carrier parents, mapping the disease to a wide 6 cM region on bovine chromosome 3 (p < 10−7). Whole genome re-sequencing of three affected calves and three putative non-carrier parents identified a novel missense variant (c.149G>A|p.Cys50Tyr) in exon 2 of the endothelin 2 (EDN2) gene. Bioinformatic analyses of p.Cys50Tyr effects predicted them to be damaging for both the structure and the function of the edn2 protein, and to create a new site of splicing that may also affect the pattern of pre-mRNA splicing and exon definition. Sanger sequencing of this variant on the rest of the sample set confirmed the segregation pattern obtained with whole genome re-sequencing. The identification of the causative variant and the development of a diagnostic genetic test enable the efficient design of matings to keep the effective population size as high as possible, as well as providing insights into the first EDN2-associated hereditary disease in cattle or other species.
- PublicationEvaluación de Quizizz y Edpuzzle para incrementar la participación del alumnado y la interacción profesor-alumnoSevane Fernández, NataliaEl objetivo de este proyecto de innovación docente es seleccionar, validar y consolidar el uso de dos herramientas docentes en abierto, Quizizz y Edpuzzle, para incrementar la participación de los estudiantes y la interacción profesor-alumno.