Person: Rebollada Merino, Agustín Miguel
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First Name
Agustín Miguel
Last Name
Rebollada Merino
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Medicina y Cirugía Animal
Area
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18 results
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Now showing 1 - 10 of 18
Item Effects on Intestinal Mucosal Morphology, Productive Parameters and Microbiota Composition after Supplementation with Fermented Defatted Alperujo (FDA) in Laying Hens(Antibiotics, 2019) Rebollada Merino, Agustín Miguel; Bárcena Asensio, Carmen; Ugarte Ruiz, María; Porras González, Néstor; Mayoral Alegre, Francisco Jesús; Tomé Sánchez, Irene; Rodríguez Bertos, Antonio Manuel; Domínguez Rodríguez, Lucas JoséThe olive oil sector is currently adapting its traditional function to also become a supplier of high-value by-products that possess antioxidant, anti-inflammatory and antimicrobial properties. In this study, we evaluated the effect of the fermented defatted alperujo (FDA) on the intestinal health of laying hens. The morphology of the duodenal and cecal mucosa, the composition of the intestinal microbiota and the productivity of a batch of laying hens were evaluated after FDA supplementation. At early life stages, significant differences (p < 0.001) were observed in duodenal villi height and in crypt depth of both the duodenum and the cecum in the FDA-supplemented group, indicating improved intestinal health in this group. Microbiota composition in the hatchery group supplemented with FDA had a higher abundance of Actinobacteria, Firmicutes and Proteobacteria, and higher bacterial diversity. During the production period, significant differences (p < 0.05) were observed in the number of broken eggs from the supplemented group. We conclude that FDA supplementation improves the absorption capacity of the intestinal mucosa and modifies the intestinal microbiota to favor a greater immune response, leading to an increase in egg production.Item An Outbreak of Aeromonas salmonicida in Juvenile Siberian Sturgeons (Acipenser baerii)(Animals, 2023) Vázquez Fernández, Esther; Chinchilla Rodríguez, Blanca; Rebollada Merino, Agustín Miguel; Domínguez Rodríguez, Lucas José; Rodríguez Bertos, Antonio ManuelAeromonas salmonicida is one of the major threats to world aquaculture, causing fish furunculosis and high mortality rates in cultured fish, particularly salmonids. Although Aeromonas spp. is a thoroughly studied pathogen, little is known regarding aeromoniasis in sturgeons. After a mortality outbreak, four juvenile sturgeons (Acipenser baerii) were submitted for autopsy and tissue samples were collected for histopathological and microbiological studies. The external examination revealed size heterogenicity, skin hyperpigmentation and reduced body condition of sturgeons. Within the abdominal cavity, mild hepatomegaly and splenomegaly were observed, as well as generalized organic congestion. Histology revealed severe multifocal haemorrhagic and ulcerative dermatitis, mainly localized in the dorsal and latero-ventral areas of fish. The histological study also showed moderate to severe inflammation of gills and organic lesions compatible with septicaemia. Bacterial isolates were identified as Aeromonas salmonicida subsp. salmonicida using MALDI-TOF MS and PCR. Overall, the lesions first described here are consistent with those previously reported in other cultured fish species and contribute to a better understanding of the pathogenesis of Aeromonas salmonicida subsp. salmonicida in the Siberian sturgeon, aside from providing new diagnostic tools for bacterial diseases impacting the fast-growing industry of caviar.Item Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis(Transboundary and Emerging Diseases, 2024) Rebollada Merino, Agustín Miguel; Martínez Alares, Irene; Duque, Clara; García-Seco Romero, María Teresa; Escacena, Cristina; Domínguez Rodríguez, Lucas José; Rodríguez Bertos, Antonio Manuel; García Benzaquén, Nerea; Daniel DiazBrucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were collected during a 6-year period, pooled, and screened for Brucella spp. by PCR. Aiming to correlate Brucella spp. DNA presence in ticks with Brucella spp. infections in wildlife ungulate hosts, liver samples from deceased wildlife ungulates coming from the hunting reserves showing a positive result for Brucella in ticks were tested using a commercial ELISA. In total, 229 tick pools from wild boar (Sus scrofa, n = 176; 76.8%, 95% CI 70.9%–81.8%), red deer (Cervus elaphus, n = 40; 17.4%, 95% CI 13.1%–22.9%), mouflon (Ovis orientalis musimon, n = 7; 3.06%, 95% CI 1.49%–6.17%), and fallow deer (Dama dama, n = 6; 2.62%, 95% CI 1.21%–5.60%) were analyzed. PCR results showed that 3.93% (95% CI 2.08%–7.30%) tick pools (9/229) from 16.6% hunting reserves (7/41) screened yielded a positive PCR result for Brucella. All positive ticks were Dermacentor (Dermacentor marginatus or Dermacentor reticulatus) collected from wild boar. Ticks collected from wild boars were positive to Brucella in a relative percentage of 5.10% (95% CI = 1.61–11.4) in 2018 and of 7.59% (95% CI = 2.79–15.6) in 2021 (6-year prevalence of 5.17%, 9/176). ELISA showed positive results in three wild boars coming from two out of seven hunting reserves (28.5%) with a positive PCR for Brucella in ticks. To conclude, Brucella spp. DNA can be detected in Dermacentor ticks parasitizing wild boars living in hunting reserves harboring Brucella spp.-seropositive wild boars. This study provides evidence that the contribution of arthropod vectors should be considered in the epidemiology of brucellosis in wildlife.Item First detection of Flavobacterium psychrophilum in juvenile Siberian sturgeons (Acipenser baerii) and description of the pathological findings(Journal of fish diseases, 2023) Chinchilla Rodríguez, Blanca; Vázquez Fernández, Esther; Rebollada Merino, Agustín Miguel; Pérez Sancho, Marta; Domínguez Rodríguez, Lucas José; Rodríguez Bertos, Antonio ManuelFlavobacterium psychrophilum affects many cultured fish species and is considered one of the most important bacterial pathogens causing substantial economic losses in salmonid aquaculture worldwide. Here, F. psychrophilum was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nested PCR as the aetiological agent causing mortality in diseased juvenile Siberian sturgeons (Acipenser baerii) reared on a freshwater fish farm. Diseased sturgeons were lethargic and displayed dark skin pigmentation, increased mucus production and the presence of skin ulcerations and haemorrhages specially on the ventral side and the base of fins. The histological examination of fish revealed proliferative branchitis, ulcerative and necrotizing dermatitis and myositis, lymphoid tissue atrophy, liver and kidney degeneration and thrombosis. To the best of our knowledge, this is the first report describing the infection of Siberian sturgeons by F. psychrophilum. The detection of F. psychrophilum in diseased Siberian sturgeons and the description of the pathological findings observed during the outbreak may contribute to a better understanding of the bacterium pathogenicity and the range of fish species susceptible to infection.- Project number: 428
Item GROSSPath 3D: Principales lesiones macroscópicas de decomiso en matadero en tres dimensiones(2021) Rodríguez Bertos, Antonio Manuel; González Huecas, Marta; Hernández Carrillo, Javier; Martín Andrés, Carlos; Mayoral Alegre, Francisco José; Pérez Pérez, Antonio; Pizarro Díaz, Manuel; Porras González, Néstor; Rebollada Merino, Agustín MiguelEn GROSSPath 3D hemos creado modelos 3D de las lesiones observadas en los órganos decomisados en el matadero, centrado en las principales lesiones de decomiso en el cerdo para generar imágenes 3D que puedan resultar de interés para los alumnos de Grado en Veterinaria. En estos casos hemos incluido la descripción macroscópica de la lesión, el diagnóstico morfológico anatomopatológico y una imagen histológica característica del proceso con la causa etiológica microbiológica tanto en español como en inglés. Con el fin de establecer colaboraciones futuras con los mataderos, que son nuestra principal fuente de lesiones en los animales de abasto, hemos facilitado el acceso a la web a los veterinarios inspectores para que determinen su utilidad, como herramienta formativa para los veterinarios sustitutos o en formación del matadero. Además, conseguir fomentar su interés en contribuir de forma permanente al desarrollo de esta página web GROSSPath 3D mediante el envío de órganos decomisados, especialmente en otras especies como pequeños rumiantes, bovino y equino. Item Environment and Offspring Surveillance in Porcine Brucellosis(Frontiers in Veterinary Science, 2022) Rebollada Merino, Agustín Miguel; Pérez Sancho, Marta; Rodríguez Bertos, Antonio Manuel; García Benzaquén, Nerea; Martínez Alares, Irene; Navarro Gómez, Alejandro; Domínguez Rodríguez, Lucas José; García-Seco Romero, María TeresaPorcine brucellosis, caused by Brucella suis (B. suis), is a notifiable disease causing significant economic losses in production systems.Most infected pigsmay act as carriers and shed B. suis even if asymptomatic. This can contribute to environmental persistence, thus hindering control efforts. Here, the environment and the offspring were investigated during and after a B. suis outbreak at a sow breeding farm. The diagnosis of B. suis in sows (n = 1,140) was performed by culture and polymerase chain reaction (PCR) from vaginal swabs, indirect enzyme-linked immunosorbent assay (I-ELISA) from sera, and brucellin skin test (BST). B. suis diagnosis in post-weaning pigs (n = 899) was performed by I-ELISA in sera and BST. The environmental surveillance programme was implemented by placing gauze sponges (n = 175) pre-hydrated in a surfactant and inactivating liquid for Brucella DNA detection by PCR in different farm areas. Our results showed that the offspring of infected sows reacted to in vivo techniques for B. suis. Furthermore, the offspring born during the outbreak displayed higher seropositivity (I- ELISA) and reactivity (BST) than those pigs born after. Brucella DNA was detected in pregnant sow areas, boxes, boots, and post-weaning pig areas. In addition, Brucella DNA environmental detection was higher during the B. suis outbreak than the post B. suis outbreak. The environmental approach has proven to be a simple, practical, valuable, and safe method to detect and monitor B. suis. These results suggest a role of the environment and the offspring that should be considered in porcine brucellosis surveillance and control programmes.Item Extracellular matrix proteins (fibronectin, collagen III, and collagen I) immunoexpression in goat tuberculous granulomas (Mycobacterium caprae)(Veterinary Research Communications, 2022) Neila, Carlos; Rebollada Merino, Agustín Miguel; Bezos Garrido, Javier; Juan Ferré, Lucía De; Rodríguez Bertos, Antonio Manuel; Domínguez Rodríguez, Lucas JoséThe lesion resulting from the interaction between Mycobacterium and the host immune response is the tuberculous granuloma. Tuberculous granulomas, except in incipient stages, are partially or totally encapsulated by connective tissue. The aim of this study was to assess the immunoexpression of the extracellular matrix proteins fibronectin, collagen III, and collagen I in granulomas caused by Mycobacterium caprae in goats (Capra aegagrus hircus) to understand capsule development at diferent granuloma stages. For this purpose, a retrospective study of 56 samples of tuberculous granulomas in lung (n=30) and mediastinal lymph node (n=26) from 17 goats naturally infected with M. caprae in stages I (n=15), II (n=14) and III (n=27) was carried out. Fibronectin immunoreaction was extracellular, fibrillar-reticular in the center of stage I, II and III granulomas and peripheral in stages II and III granulomas. Collagen III immunoexpression was extracellular and fibrillar in the center of stages I, II and III tuberculous granulomas in lung and mediastinal lymph node, and progressive expression was observed in the periphery of stages II and III granulomas. Finally, collagen I immunoexpression was extracellular and fibrillar, showing a progressive loss of central expression and an increase in peripheral expression in stage III granulomas compared to stage I granulomas. Immunoexpression of these extracellular matrix proteins could help understand fibrogenesis and dating in tuberculous granuloma in both animal models and humans.Item Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 Immunoexpression(Veterinary Sciences, 2022) Porras, Néstor; Rebollada Merino, Agustín Miguel; Rodríguez Franco, Fernando; Calvo-Ibbitson, Andrés; Rodríguez Bertos, Antonio ManuelFeline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) has been described as an inflammatory disorder with an eosinophilic component with etiopathogenesis that is still unknown. Sixteen intestinal samples from two veterinary diagnostic services (2014–2017) were included in the study. A histopathological criterion classified the cases into three grades (mild, moderate, and severe) according to the distribution of the lesions and the course. An immunohistochemical study of collagen I, collagen III, fibronectin, and transforming growth factor β1 (TGF-β1) was performed in each case. An immunohistochemical study of mild grades shows greater collagen III immunoexpression, compared to collagen I and fibronectin, which suggests an “early” stage of fibrosis. In more intense grades, an increased immunoexpression of collagen I, compared to collagen III, suggests a “late” stage of fibrosis. Otherwise, the highest expression of TGF-β1 was observed in the moderate phase, due to the high proliferation of reactive fibroblast and intense inflammation. The results suggest that the inflammatory infiltrate is the trigger for the elevation in TGF-β1, altering the collagen type III:I ratio. In conclusion, immunohistochemical studies can be a very useful method in diagnosing cases of FGESF of mild grades and could help to apply a differential diagnosis regarding feline eosinophilic chronic enteritis (CEE) in the context of inflammatory bowel disease (IBD).Item Forensic investigations of religious rituals involving poultry: a case report(Journal of Comparative Pathology, 2023) Vázquez Fernández, Esther; Rebollada Merino, Agustín Miguel; Chinchilla Rodríguez, Blanca; Porras González, Néstor; Rodríguez Bertos, Antonio ManuelAnimal victims of human cruelty are receiving increasing attention from the press and society. Veterinary pathologists and civic authorities have a duty not only to elucidate the cause, method and manner of death but also to address the motivation behind a case. Poultry are commonly used as offering to gods in Santería rituals (ebós). Only a few cases have been reported in scientific journals, in contrast with the number of cases described in the media. Here, a hen and a cockerel (Gallus domesticus) were submitted to the VISAVET Health Surveillance Centre for autopsy, which comprised macroscopic and histological studies and microbiological testing for Chlamydia psittaci, avian orthoavulavirus 1 (Newcastle disease virus), West Nile virus and avian influenza virus. The carcasses were in an active decay stage of decomposition, containing larvae of Lucilia eximia. Both animals had been decapitated and haemorrhages in the cutting region were observed histologically. Post-mortem burns were also observed. To the best of the authors’ knowledge, this is the first post-mortem description of lesions in a domestic avian species due to religious rituals. European Union and Spanish forensic practitioners should be aware that these ritual sacrifices occur in some European countries. Moreover, future European and national legislation updates should consider this type of animal abuse.Item Animal abuse in Spanish-breed fighting cocks (Gallus gallus domesticus)(Forensic Science International: Animals and Environments, 2022) Rebollada Merino, Agustín Miguel; Bárcena Asensio, María Carmen; Barreno San Antolín, Lucía; Pizarro Díaz, Manuel; Domínguez Rodríguez, Lucas José; Rodríguez Bertos, Antonio ManuelCockfighting is illegal both in the European Union and the United States, although fighting cock husbandry and breed preservation are allowed. Here, we describe the pathological findings observed in the carcasses of two Spanish-breed fighting cocks submitted to our laboratory after an animal abuse-suspected death. The postmortem examination revealed these cocks may have been used for cockfighting (comb, ear lobes, wattles, and chest feather removal; and trimmed spurs). The severe extensive hemorrhages observed in the cervical region of both birds, affecting C1-C4 in bird No. 1 and C8-C11 in No. 2, suggested cervical dislocation in a non-professional manner. To the best of our knowledge, this is the first report of abuse in a Spanish-breed fighting cock. The aim of this paper is to contribute to the identification of animal abuse cases in avian species which may lead to successful prosecutions in Spain and elsewhere.