Person:
Andreu Rodríguez, José Manuel

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First Name
José Manuel
Last Name
Andreu Rodríguez
URI
https://hdl.handle.net/20.500.14352/80118
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Psicología
Department
Personalidad, Evaluación y Psicología Clínica
Area
Personalidad, Evaluación y Tratamiento Psicológico
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2013 - 20173
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End date: 2019 × Start date: 2010 ×

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Now showing 1 - 3 of 3
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    The structural assembly switch of cell division protein FtsZ probed with fluorescent allosteric inhibitors
    (Chemical Science, 2017) Artola Pérez de Azanza, Marta Elena; Ruiz Ávila, Laura, Laura B.; Ramírez Aportela, Erney; Fernando Martínez, R.; Araujo Bazán, Lidia; Vázquez Villa, Henar; Martín Fontecha, María del Mar; Oliva Blanco, María Ángela; Martín Galiano, Antonio Javier; Chacón Montes, Pablo; López Rodríguez, María Luz; Andreu Rodríguez, José Manuel; Huecas Gayo, Sonia
    FtsZ is a widely conserved tubulin-like GTPase that directs bacterial cell division and a new target for antibiotic discovery. This protein assembly machine cooperatively polymerizes forming single-stranded filaments, by means of self-switching between inactive and actively associating monomer conformations. The structural switch mechanism was proposed to involve a movement of the C-terminal and N-terminal FtsZ domains, opening a cleft between them, allosterically coupled to the formation of a tight association interface between consecutive subunits along the filament. The effective antibacterial benzamide PC190723 binds into the open interdomain cleft and stabilizes FtsZ filaments, thus impairing correct formation of the FtsZ ring for cell division. We have designed fluorescent analogs of PC190723 to probe the FtsZ structural assembly switch. Among them, nitrobenzoxadiazole probes specifically bind to assembled FtsZ rather than to monomers. Probes with several spacer lengths between the fluorophore and benzamide moieties suggest a binding site extension along the interdomain cleft. These probes label FtsZ rings of live Bacillus subtilis and Staphylococcus aureus, without apparently modifying normal cell morphology and growth, but at high concentrations they induce impaired bacterial division phenotypes typical of benzamide antibacterials. During the FtsZ assembly-disassembly process, the fluorescence anisotropy of the probes changes upon binding and dissociating from FtsZ, thus reporting open and closed FtsZ interdomain clefts. Our results demonstrate the structural mechanism of the FtsZ assembly switch, and suggest that the probes bind into the open clefts in cellular FtsZ polymers preferably to unassembled FtsZ in the bacterial cytosol.
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    Project number: 14
    Mindfulness aplicado al aula universitaria
    (2017) Andreu Rodríguez, José Manuel; Peña Fernández, María Elena; Graña Gómez, José Luis; Redondo Rodríguez, Natalia; Cuenca Montesino, María Luisa; Sáez Cutando, Cristina
    El Mindfulness, literalmente traducido como atención o conciencia plena, es una de las múltiples formas de meditación que se basa en centrar la mente en el momento presente. En otras palabras, es un estado de conciencia que se desarrolla prestando una atención concreta, sostenida y deliberada sin juzgar las experiencias del aquí y del ahora. La mayoría de los estudios sobre los efectos de la práctica continuada del Mindfulness se han realizado en contextos clínicos y laborales. Se disponen de suficientes resultados que muestran que los beneficios del Mindfulness están relacionados con la salud, el bienestar psicológico, las competencias sociales o el rendimiento académico de niños y adolescentes. Sin embargo, son pocos los estudios que se han llevado a cabo en el contexto educativo universitario y, más específicamente, en relación con estudiantes universitarios.
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    Recharge estimation of a small karstic aquifer in a semiarid Mediterranean region (southeastern Spain) using a hydrological mode.
    (Hydrological processes, 2013) Touhami, Issam; Andreu Rodríguez, José Manuel; Chirino, E.; Sánchez Sutil, J. R.; Moutahir, H.; Pulido Bosch, A.; Martínez Santos, Pedro; Bellot, J.
    Mediterranean aquifers are frequently subject to the joint effect of intensive exploitation and low recharge values. Besides, groundwater is the only available water resource in many Mediterranean regions. Groundwater recharge studies are therefore necessary to underpin water management practices. This manuscript presents a methodology to estimate groundwater recharge in a small limestone aquifer of southeastern Spain. The HYDROBAL model is used to calculate daily soil water balances on the basis of hydrological and soil data as well as on vegetation cover. Deep drainage model outputs are converted into water table variations using a lumped model add‐on. The adjustment between observed and calculated water table levels is in the order of r2 = 0.87. This correlation coefficient suggests that HYDROBAL is a useful tool to estimate groundwater recharge in the region. In addition, differences in groundwater recharge rates are observed for dry, average and wet years. Estimated recharge rates range between 0% and 18% of the mean annual rainfall, which corresponds to a net recharge of 0 to 59 mm year−1. Recharge rates increase proportionally with precipitation (r 2 = 0.90).