Martín Sabroso, Cristina

Profile Picture
First Name
Last Name
Martín Sabroso
Universidad Complutense de Madrid
Faculty / Institute
Farmacia Galénica y Tecnología Alimentaria
Farmacia y Tecnología Farmaceútica
UCM identifierORCIDScopus Author IDWeb of Science ResearcherIDDialnet ID

Search Results

Now showing 1 - 10 of 18
  • Publication
    Embolization therapy with microspheres for the treatment of liver cancer: State-of-the-art of clinical translation
    (Elsevier, 2022-08-10) Pérez López, Alexandre; Martín Sabroso, Cristina; Gómez Lázaro, Laura; Torres Suárez, Ana Isabel; Aparicio Blanco, Juan
    Embolization with microspheres is a therapeutic strategy based on the selective occlusion of the blood vessels feeding a tumor. This procedure is intraarterially performed in the clinical setting for the treatment of liver cancer. The practice has evolved over the last decade through the incorporation of drug loading ability, biodegradability and imageability with the subsequent added functionality for the physicians and improved clinical outcomes for the patients. This review highlights the evolution of the embolization systems developed through the analysis of the marketed embolic microspheres for the treatment of malignant hepatocellular carcinoma, namely the most predominant form of liver cancer. Embolic microspheres for the distinct modalities of embolization (i.e., bland embolization, chemoembolization and radioembolization) are here comprehensively compiled with emphasis on material characteristics and their impact on microsphere performance. Moreover, the future application of the embolics under clinical investigation is discussed along with the scientific and regulatory challenges ahead in the field.
  • Publication
    PLGA Nanoparticles for the Intraperitoneal Administration of CBD in the Treatment of Ovarian Cancer: In Vitro and In Ovo Assessment
    (MDPI, 2020-05-09) Fraguas Sánchez, Ana Isabel; Torres Suárez, Ana Isabel; Cohen, Marie; Delie, Florence; Bastida-Ruiz, Daniel; Yart, Lucile; Martín Sabroso, Cristina; Fernández Carballido, Ana María
    The intraperitoneal administration of chemotherapeutics has emerged as a potential route in ovarian cancer treatment. Nanoparticles as carriers for these agents could be interesting by increasing the retention of chemotherapeutics within the peritoneal cavity. Moreover, nanoparticles could be internalised by cancer cells and let the drug release near the biological target, which could increase the anticancer efficacy. Cannabidiol (CBD), the main nonpsychotropic cannabinoid, appears as a potential anticancer drug. The aim of this work was to develop polymer nanoparticles as CBD carriers capable of being internalised by ovarian cancer cells. The drug-loaded nanoparticles (CBD-NPs) exhibited a spherical shape, a particle size around 240 nm and a negative zeta potential (−16.6 ± 1.2 mV). The encapsulation efficiency was high, with values above 95%. A controlled CBD release for 96 h was achieved. Nanoparticle internalisation in SKOV 3 epithelial ovarian cancer cells mainly occurred between 2 and 4 h of incubation. CBD antiproliferative activity in ovarian cancer cells was preserved after encapsulation. In fact, CBD-NPs showed a lower IC50 values than CBD in solution. Both CBD in solution and CBD-NPs induced the expression of PARP, indicating the onset of apoptosis. In SKOV-3-derived tumours formed in the chick embryo model, a slightly higher—although not statistically significant tumour growth inhibition was observed with CBD-NPs compared to CBD in solution. To sum up, poly-lactic-co-glycolic acid (PLGA) nanoparticles could be a good strategy to deliver CBD intraperitoneally for ovarian cancer treatment.
  • Publication
    DoE-based development of celecoxib loaded PLGA nanoparticles: In ovo assessment of its antiangiogenic effect
    (Elsevier, 2022-10-08) Alonso González, Mario; Quispe Chauca, Prissila; Fernández Carballido, Ana María; Lozza, Irene; Martín Sabroso, Cristina; Fraguas Sánchez, Ana Isabel
    Abnormal angiogenesis plays a main role in the pathogenesis of many diseases such as cancer, and inflammatory autoimmune disorders among others, and its inhibition represents a potential strategy for their management. Celecoxib (CXB) that is one of the most prescribed selective COX-2 inhibitors and is currently approved for the treatment of osteoarthritis, rheumatoid arthritis, and ankylosing spondylitis inhibits angiogenesis. The objective of this manuscript was to design, develop, and characterize polymeric nanoparticles for the parenteral administration of CXB which the aim of facilitating its administration and improving its antiangiogenic activity while decreasing its adverse effects. A Plackett-Burman design was used to optimize the formulation. The PVA concentration, the sonication time, the sonicator amplitude and the CXB:PLGA ratio were selected as independent variables and particle size, polydispersity index, drug loading, and entrapment efficiency as responses. Optimized nanoparticles (formulations F2, F6 and F9) showed a particle size around 280 nm, a low polydispersion (PDI ≤ 0.2), a negative zeta potential around -25mV, a high entrapment efficiency (above 88%) and a controlled drug release for at least 10 days. Moreover, they were physically and chemically stable for at least 3 months when stored at 4°C. Interestingly, CXB-loaded nanoparticles showed a higher angiogenesis inhibition than CXB in solution administered at the same concentration. F9 nanoparticles that were prepared using PVA at 0.5%, a sonication time of 7 minutes, a sonicator amplitude of 80% and a CXB:PLGA ratio of 20:100 were selected as the most suitable CXB-formulation. It represents a promising strategy to administer CXB and improve its efficacy in disorders with pathological angiogenesis such as cancer and arthritic diseases.
  • Publication
    Desarrollo de micropartículas de dexametasona como estrategia para aumentar la eficacia de los esquemas de poliquimioterapia
    (Universidad Complutense de Madrid, 2014-03-27) Martín Sabroso, Cristina; Torres Suárez, Ana Isabel
    Glucocorticoids (GCs) and dexamethasone play a central role in the treatment of lymphoid malignancies, particularly acute lymphoblastic leukaemia (ALL). Preclinical studies have shown that GCs also affect cell differentiation, proliferation and apoptosis of osteosarcoma cells, hepatoma cells, mammary tumor cells, glioma cells, melanoma cells and thyroid cancer cells. The proliferation and apoptotic effects of GCs are cell type-specific as well as time and concentration dependent. In spite of the high effectiveness of dexamethasone treatment in ALL, mainly in children, GC resistance occurs in 10-30% of untreated patients, being more frequent in T-lineage than B-precursor acute lymphoblastic leukaemia. Furthermore, systemic administration of high doses of dexamethasone are required for inducing tumor cell apoptosis but causes severe side effects such as osteoporosis, Cushing's syndrome or an increased risk of infections. Even though most combined chemotherapy protocols currently used in clinics include dexamethasone at high doses, recent clinical investigations on the eficacy of dexamethasone in refractory multiple myeloma have shown synergic effects of low doses of this GC combined with pomalidomide or lenalidomide...
  • Publication
    Trivial-to-Learn: Aprendizaje basado en juegos en el área de conocimiento de Farmacia y Tecnología Farmacéutica (TRIVIALFAR)
    Fraguas Sánchez, Ana Isabel; Fernández Carballido, Ana María; Garcia Cremades-Mira, María; Martín Sabroso, Cristina; Torres Suárez, Ana Isabel; Aparicio Blanco, Juan; Negro Alvarez, María Sofía Elisa; Barcia Hernández, Emilia; Córdoba Díaz, Damián; Lozza, Irene; Gómez Lázaro, Laura; Pérez López, Alexandre; Gómez García, Víctor; Catalan Pallares, Sandra; Rodriguez Sanchez, Rafael
    La sociedad actual demanda a las Instituciones Educativas profesionales que tengan una formación integral que les permita adaptarse al entorno y a los requerimientos sociolaborales. Para ello, es esencial el empleo de metodologías docentes activas, como el aprendizaje basado en juegos, donde el estudiante es el protagonista del proceso de enseñanza-aprendizaje. El objetivo principal de este proyecto es diseñar y desarrollar juegos tipo Trivial como estrategia de aprendizaje activo en las asignaturas del área de conocimiento de Farmacia y Tecnología Farmacéutica. Concretamente se han desarrollado dos tipos de actividades: juegos tipo Trivial virtuales en la asignatura de Biofarmacia y Farmacocinética, y juegos tipo Trivial presenciales en las asignaturas de Productos Sanitarios y Tecnología Farmacéutica I. Ambas actividades han resultado ser una excelente herramienta de autoevaluación y repaso de los contenidos impartidos en la asignatura. Los juegos desarrollados en modalidad presencial fueron mejor evaluados por los alumnos que los desarrollados en modalidad virtual. Además, estos juegos presenciales les permitía adquirir competencias transversales, principalmente habilidades comunicativas, de liderazgo y de trabajo colaborativo.
  • Publication
    Capacitación en la generación de contenidos divulgativos de materias de Ciencias de la Salud para su difusión en entornos virtuales: aplicación en el área de conocimiento de Farmacia y Tecnología Farmacéutica (GEDITEFAR)
    (2022-10-31) Aparicio Blanco, Juan; Barcia Hernández, Emilia; Catalán Pallarés, Sandra; Córdoba Díaz, Damián; Fernández Carballido, Ana María; Fraguas Sánchez, Ana Isabel; Martín Sabroso, Cristina; Negro Alvarez, María Sofía Elisa; Notivoli Díez, Pablo; Pérez López, Alexandre; Rodríguez Sánchez, Rafael; Tapias Frutos, Beatriz; Torres Suárez, Ana Isabel
    La necesidad de visibilizar los objetivos sociales del esfuerzo científico ha quedado patente ante la actual pandemia por SARS-CoV-2, en un contexto que ha puesto en valor la necesidad de comunicar a la población una terminología y conocimientos sanitarios básicos sobre temas de interés general. En este sentido, los alumnos de los Grados en Ciencias de la Salud deben estar capacitados para generar dichos contenidos divulgativos. El objetivo de este proyecto se enmarca en dicha capacitación aplicada a las asignaturas del área de Farmacia y Tecnología Farmacéutica impartidas en el Grado en Farmacia y en el Doble Grado en Farmacia y Nutrición Humana y Dietética. Así, el proyecto se enfoca en la generación de material divulgativo sobre problemas sanitarios reales de actualidad y relacionados con los contenidos de las asignaturas Tecnología Farmacéutica I, Biofarmacia y Farmacocinética, Tecnología Farmacéutica II y Productos Sanitarios (impartidas entre los cursos tercero y cuarto y repartidas homogéneamente en los dos cuatrimestres). El objetivo principal es incentivar el aprendizaje de dichas materias a través de la aplicación de los conocimientos adquiridos a la identificación, análisis y resolución de problemas sanitarios reales, mediante la utilización de una metodología de aprendizaje basada en retos, todo ello guiado y supervisado por los profesores implicados en el proyecto y apoyado en una bibliografía específica y sólida. Además, se pretende concienciar a los alumnos de la dimensión social de la profesión farmacéutica en el contexto de las Ciencias de la Salud y de la relevancia de las habilidades de comunicación para las diversas modalidades del ejercicio profesional (tan importantes en ámbitos como la farmacia comunitaria o la farmacia hospitalaria). A fin de hacer más atractivos a los alumnos los canales de difusión de los contenidos generados por ellos mismos y ante la emergente digitalización de la población general experimentada durante la última anualidad, se emplean algunas de las tecnologías de la información y comunicación disponibles. En concreto, se ha obtenido un dominio externo que soporte la inclusión de un blog en el que se puedan habilitar plugins de registro de actividad como ejes vertebradores de los espacios virtuales de difusión. Asimismo, se han creado perfiles en redes sociales específicos del proyecto para contribuir a la difusión de los contenidos disponibles en el espacio web anterior (Twitter e Instagram).
  • Publication
    Estabilidad y conservación de los medicamentos: aprendizaje basado en retos para la correcta conservación de los medicamentos por parte de la población
    (2020-07-01) Martín Sabroso, Cristina; Córdoba Díaz, Damián; Barcia Hernández, Emilia María; Negro Alvarez, María Sofía Elisa; Fraguas Sánchez, Ana Isabel; Alonso González, Mario; Andrés Yagüe, Ana María; Slowing Barillas, Karla Veronica; Fernández Carballido, Ana María; Torres Suárez, Ana Isabel
    El proyecto, enfocado en la estabilidad y conservación de los medicamentos, pretende facilitara a los alumnos el aprendizaje de la materia Tecnología Farmacéutica II impartida en cuarto curso del grado de Farmacia, aproximando los conocimientos adquiridos a la identificación, análisis y resolución de problemas sanitarios reales, mediante la utilización de una metodología de aprendizaje basado en retos.
  • Publication
    Limitations and Challenges in the Stability of Cysteamine Eye Drop Compounded Formulations
    (MDPI, 2022-12-21) Martín Sabroso, Cristina; Alonso González, Mario; Fernández Carballido, Ana María; Aparicio Blanco, Juan; Córdoba Díaz, Damián; Navarro García, Federico; Córdoba Díaz, Manuel; Torres Suárez, Ana Isabel
    Accumulation of cystine crystals in the cornea of patients suffering from cystinosis is considered pathognomonic and can lead to severe ocular complications. Cysteamine eye drop compounded formulations, commonly prepared by hospital pharmacy services, are meant to diminish the build-up of corneal cystine crystals. The objective of this work was to analyze whether the shelf life proposed for six formulations prepared following different protocols used in hospital pharmacies is adequate to guarantee the quality and efficacy of cysteamine eye drops. The long-term and in-use stabilities of these preparations were studied using different parameters: content of cysteamine and its main degradation product cystamine; appearance, color and odor; pH and viscosity; and microbiological analysis. The results obtained show that degradation of cysteamine was between 20% and 50% after one month of storage in the long-term stability study and between 35% and 60% in the in-use study. These data confirm that cysteamine is a very unstable molecule in aqueous solution, the presence of oxygen being the main degradation factor. Saturation with nitrogen gas of the solutions offers a means of reducing cysteamine degradation. Overall, all the formulae studied presented high instability at the end of their shelf life, suggesting that their clinical efficacy might be dramatically compromised.
  • Publication
    Overcoming Glucocorticoid Resistances and Improving Antitumor Therapies: Lipid and Polymers Carriers
    (2014-09-12) Martín Sabroso, Cristina; A. J. Moreno-Ortega; Aparicio Blanco, Juan; Fraguas Sánchez, Ana Isabel; M. F. Cano-Abad; Torres Suárez, Ana Isabel
    Purpose To improve chemotherapy protocols of lymphoid malignancies, by using polymeric and lipid microparticles as controlled delivery systems of dexamethasone, part of all combined chemotherapy protocols for its strong-inducing effect on malignant lymphoblasts. Methods Polymeric microparticles were prepared by the oil-in-water-emulsion cosolvent evaporation method, andlipid microparticles by spray drying. Their cytotoxic effects on GC-sensitive PC12 cells and GC-resistant PC3 cells were characterized by cell proliferation and apoptosis assays. Results Both elaboration methods rendered optimal-sized microparticles for parenteral administration with high drug loading. In vitro assays showed sustained dexamethasone release from polymeric microparticles over a month, whereas 100% dexamethasone release from lipid microparticles was achieved within 24 h. Similar PC12 cell death to that obtained with dexamethasone solution administered every 48 h was achieved with dexamethasone polymeric microparticles in 26-days assays. Dexamethasone solution and loaded polymeric microparticles induced apoptosis around 15.8 and 19.9%, respectively, after 2 days of incubation. Lipid microparticles increased further apoptosis induction in PC12 cells and, unlike dexamethasone solution and polymeric microparticles, showed antiproliferative effects on PC3 cells. Conclusions Dexamethasone polymeric microparticles constitute an alternative to current dexamethasone administration systems in combined chemotherapy, whereas dexamethasone lipid microparticles represent a potential tool to revert glucocorticoid resistance.
  • Publication
    In vitro screening of nanomedicines through the blood brain barrier: A critical review
    (Elsevier, 2016-10) Aparicio Blanco, Juan; Martín Sabroso, Cristina; Torres Suárez, Ana Isabel
    The blood-brain barrier accounts for the high attrition rate of the treatments of most brain disorders, which therefore remain one of the greatest health-care challenges of the twenty first century. Against this background of hindrance to brain delivery, nanomedicine takes advantage of the assembly at the nanoscale of available biomaterials to provide a delivery platform with potential to raising brain levels of either imaging or therapeutic agents. Nevertheless, to prevent later failure due to ineffective drug levels at the target site, researchers have been endeavoring to develop a battery of in vitro screening procedures that can predict earlier in the drug discovery process the ability of these cutting-edge drug delivery platforms to cross the blood-brain barrier for biomedical purposes. This review provides an in-depth analysis of the currently available in vitro blood-brain barrier models (both cell-based and non-cell-based) with the focus on their suitability for understanding the biological brain distribution of forthcoming nanomedicines. The relationship between experimental factors and underlying physiological assumptions that would ultimately lead to a more predictive capacity of their in vivo performance, and those methods already assayed for the evaluation of the brain distribution of nanomedicines are comprehensively discussed.