Person: Jara Pérez, Josué
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First Name
Josué
Last Name
Jara Pérez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Nutrición y Ciencia de los Alimentos
Area
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5 results
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Item Expansion microscopy applied to mono- and dual-species biofilms(Biofilms and Microbiomes, 2023) Valdivieso González, David; Jara Pérez, Josué; Almendro Vedia, Víctor Galileo; Orgaz Martín, Belén; López Montero, IvánExpansion microscopy (ExM) is a new super-resolution technique based on embedding the biological sample within a hydrogel and its physical expansion after swelling. This allows increasing its size by several times while preserving its structural details. Applied to prokaryotic cells, ExM requires digestion steps for efficient expansion as bacteria are surrounded by a rigid cell wall. Furthermore, bacteria can live in social groups forming biofilms, where cells are protected from environmental stresses by a self-produced matrix. The extracellular matrix represents an additional impenetrable barrier for ExM. Here we optimize the current protocols of ExM and apply them to mono- and dual-species biofilms formed by clinical isolates of Limosilactobacillus reuteri, Enterococcus faecalis, Serratia marcescens and Staphylococcus aureus. Using scanning electron microscopy for comparison, our results demonstrate that embedded bacteria expanded 3-fold. Moreover, ExM allowed visualizing the three-dimensional architecture of the biofilm and identifying the distribution of different microbial species and their interactions. We also detected the presence of the extracellular matrix after expansion with a specific stain of the polysaccharide component. The potential applications of ExM in biofilms will improve our understanding of these complex communities and have far-reaching implications for industrial and clinical research.Item Investigating the Metabolism of Estrogens in Ligilactobacillus salivarius Strains Isolated from Human Milk and Vaginal Microbiota(Nutrients, 2024) Aragón, Alberto; Jurado Escobar, Rubén; Jara Pérez, Josué; Rodríguez Gómez, Juan Miguel; Orgaz Martín, BelénThe interplay between enterohepatic circulation and the gut microbiota is the main driver determining systemic levels of estrogens and their metabolites. Nevertheless, the role of potentially probiotic microorganisms in estrogen metabolism has not been investigated so far. In this work, we have explored the ability of six Ligilactobacillus salivarius strains isolated from human milk and vaginal samples to degrade and/or conjugate parental estrogens in vitro and under aerobic conditions. The quantification of estrogens and their derivatives was carried out in cell-free supernatants by LCQQQ-MS. All the tested L. salivarius strains achieved an average degradation rate of estrone and estriol of 98% and 55%, respectively, whereas 17β-estradiol was preferentially conjugated (up to 40%). The presence of seven out of ten genes encoding enzymes relevant for estrogen metabolism was further confirmed by PCR, highlighting their genetic potential for degrading, conjugating and/or deconjugating estrogens. The tested L. salivarius strains may be considered potential probiotics affecting the fate of endogenous estrogens. Clinical trials targeting populations with estrogendependent conditions will be required to elucidate the true potential of these strains for the restoration and maintenance of a healthy host estrobolome.Item Self-Adaptation of Pseudomonas fluorescens Biofilms to Hydrodynamic Stress(Frontiers in Microbiology, 2021) Jara Pérez, Josué; Alarcón, Francisco; Monnappa, Ajay K.; Santos, José Ignacio; Bianco, Valentino; Nie, Pin; Ciamarra, Massimo Pica; Canales, Ángeles; Dinis Vizcaíno, Luis Ignacio; López-Montero, Iván; Valeriani, Chantal; Orgaz Martín, BelénIn some conditions, bacteria self-organize into biofilms, supracellular structures made of a self-produced embedding matrix, mainly composed of polysaccharides, DNA, proteins, and lipids. It is known that bacteria change their colony/matrix ratio in the presence of external stimuli such as hydrodynamic stress. However, little is still known about the molecular mechanisms driving this self-adaptation. In this work, we monitor structural features of Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress. Our measurements show that the hydrodynamic stress concomitantly increases the cell density population and the matrix production. At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents. Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies. This results in the formation of more mechanically stable biofilms due to an increase of the number of crosslinks, as shown by computer simulations. The mechanical stability also relies on a change in the chemical compositions of the matrix, which becomes enriched in carbohydrates, known to display adhering properties. Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.Item Linking preterm infant gut microbiota to nasograstric enteral feeding tubes: exploring potential interactions and microbial strain transmission(Frontiers in Pediatrics, 2024) Jara Pérez, Josué; Alba Rubio, Claudio; Del Campo, Rosa; Fernández Álvarez, Leonides; Sáenz de Pipaón, M.; Rodríguez Gómez, Juan Miguel; Orgaz Martín, BelénIntroduction: Preterm birth is a growing problem worldwide. Staying at a neonatal intensive care unit (NICU) after birth is critical for the survival of preterm infants whose feeding often requires the use of nasogastric enteral feeding tubes (NEFT). These can be colonized by hospital-associated pathobionts that can access the gut of the preterm infants through this route. Since the gut microbiota is the most impactful factor on maturation of the immune system, any disturbance in this may condition their health. Therefore, the aim of this study is to assess the impact of NEFT-associated microbial communities on the establishment of the gut microbiota in preterm infants. Material and methods: A metataxonomic analysis of fecal and NEFT-related samples obtained during the first 2 weeks of life of preterm infants was performed. The potential sharing of strains isolated from the same set of samples of bacterial species involved in NICU's outbreaks, was assessed by Random Amplification of Polymorphic DNA (RAPD) genotyping. Results: In the samples taken 48 h after birth (NEFT-1 and Me/F1), Staphylococcus spp. was the most abundant genera (62% and 14%, respectively) and it was latter displaced to 5.5% and 0.45%, respectively by Enterobacteriaceae. Significant differences in beta diversity were detected in NEFT and fecal samples taken at day 17 after birth (NEFT-3 and F3) (p = 0.003 and p = 0.024, respectively). Significant positive correlations were found between the most relevant genera detected in NEFT-3 and F3. 28% of the patients shared at least one RAPD-PCR profile in fecal and NEFT samples and 11% of the total profiles were found at least once simultaneously in NEFT and fecal samples from the same patient. Conclusion: The results indicate a parallel bacterial colonization of the gut of preterm neonates and the NEFTs used for feeding, potentially involving strain sharing between these niches. Moreover, the same bacterial RAPD profiles were found in neonates hospitalized in different boxes, suggesting a microbial transference within the NICU environment. This study may assist clinical staff in implementing best practices to mitigate the spread of pathogens that could threaten the health of preterm infants.- Project number: 321
Item Integra y aprende. Construyendo una cadena de bloques (blockchain) de la granja a la mesa(2023) Cambero Rodríguez, María Isabel; Aguado Ramo, Juan Antonio; Aguilar Jaime, María Victoria; Alba Rubio, Claudio; Alonso Monge, Rebeca María Del Mar; Aragón Ramirez, Alberto; Arias López, Patricia; Arias Revenga, Jorge; Bermudez González, Guillermo; Bermejo Poza, Rubén; Blanch Rojo, María; Blanco Flores, María Dolores; Blanco Montoro, Rafael José; Bonel Ayuso, Diego Paul; Borrero Del Pino, Juan; Bugeda de Bonilla, Inés; Burgía Domínguez, Angélica; Cabeza Briales, María Concepción; Cabezas Albéniz, Almudena; Calahorra Fernández, Felipe José; Castro Madrigal, Teresa; Castro Navarro, Irma; Cervantes Navarro, Isabel; Corugedo Fernández, Lucía; Cruces Díaz, Ainhoa; Díaz Formoso, Lara; Díez Romera, Mariano; Duarete Pacheco, Sofía; Fernández Álvarez, Leonides; Fernández Solís, Claudia; Fernández-Acero Bascones, Teresa; Ferreira García-Osorio, Andrea; Fraga Perucha, Nerea; Fuente Vázquez, Jesús De La; Galicia Larrea, Paula; Gamonal Martos, Miriam; García Álvarez, Andrés; García Balboa, María Del Camino; García Calvo, Eduardo Rafael; García García, Aina; García Lacarra, Teresa; García Quiroga, Sara; García Huch, Uma Jade; González González, Noelia; González Jiménez, Lucía; Haza Duaso, Ana Isabel; Herranz Domingo, Andrea; Herranz Sorribes, Carmen; Isabel Redondo, Beatriz; Jara Pérez, Josué; Jurado Escobar, Rubén; Justo Ruiz, Carolina; Lafuente Orte, Irene; López, Cindy Alejandra; López Bote, Clemente José; López Valdeolivas, Patricia; Magro Arconada, Paula; Mallavia León, Blanca; Martín Amores, Ruth; Martín De Santos, María Del Rosario; Morales Gómez, Paloma; Moreda de Figueroa, Blanca; Moreno Conde, Helena María; Muñoz Atienza, Estefanía; Olivares Moreno, Álvaro; Peña Vidal, Nuria; Pérez Cabal, María De Los Ángeles; Pérez Sen, Raquel; Prieto Suárez, María Cinta; Paniagua Roas, Alejandra; Ramis Cervantes, Ana María; Recamal Pagán, Carlota; Remiro Yagüe, Víctor; Rodríguez Fernández, Carmina; Rodríguez Gómez, Santiago; Rodríguez Peña, José Manuel; Romera Villena, Natalia; Salazar Hijosa, Raúl; Sanabria Dominguez, Nerea; Santacruz Parra, Marta; Santos Arnaiz, Carlos; Santos López, Sergio; Torrecilla Velasco, José Santiago; Velasco De Diego, Raquel; Velasco Villar, Susana; Villanueva Suárez, María José