Person: Beroiz Remírez, Beatriz
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First Name
Beatriz
Last Name
Beroiz Remírez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Genética, Fisiología y Microbiología
Area
Genética
Identifiers
17 results
Search Results
Now showing 1 - 10 of 17
Item Genetic structure of Spanish populations of Ceratitis capitata revealed by RAPD and ISSR markers: implications for resistance management(Spanish Journal of Agricultural Research, 2012) Beroiz Remírez, Beatriz; Ortego, Félix; Callejas Hervás, Carmen; Hernández-Crespo, Pedro; Castañera, Pedro; Ochando González, María DoloresThe Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is considered one of the most economically damaging pests of citrus orchards in Spain. The characterization of C. capitata population structure, at a large geographical scale, by using a combination of RAPD and ISSR markers can allow analyzing the genetic variability of this species, and provide some insight in decision making for resistance management, recently recorded in Spain. We compared six Spanish populations along the Mediterranean area (Gerona, Amposta, Tortosa, Castellón, Valencia and Málaga) with populations from other geographical areas where this pest is widely distributed (Africa, Middle East, South America and Atlantic Islands) and two laboratory strains. The results obtained with both types of molecular markers were similar. A dendrogram based on Nei genetic distances showed that all Mediterranean Spanish populations, except the population collected in Gerona, were clearly separated from the rest. However, no clear differentiation among Spanish populations was found, probably as a result of the high levels of gene flow (Nm value of 2.8 for RAPD and 3.9 for ISSR). Implications of these findings on resistance management of C. capitata are discussed.Item Caracterización morfométrica y genética de las poblaciones españolas de cangrejo de río "Austropotamobius pallipes" (Lereboullet, 1858) mediante el uso de marcadores moleculares(2005) Beroiz Remírez, Beatriz; Ochando, Maria Dolores; Callejas Hervás, Ma. del CarmenEl cangrejo de río Austropotamobius pallipes (Lereboullet, 1858) está incluido en la Lista Roja de la U.I.C.N. (Unión Internacional para la Conservación de la Naturaleza) en la categoría de "vulnerable", al igual que en el Catálogo Nacional de especies amenazadas por su tendencia poblacional regresiva que le ha llevado a desaparecer de una gran parte de su área de distribución anterior (BOE nº 149 de 23 de junio de 2003).Dada la situación que atraviesa el cangrejo de río nos hemos planteado, mediante la utilización de la metodología RAPD-PCR y marcadores morfométricos, el estudio de sus poblaciones. La combinación simultánea de ambos marcadores permite intentar conocer a fondo la estructura poblacional de la especie A. pallipes en España. Los patrones de distribución de la variabilidad, tanto genética como morfométrica observada en estas poblaciones, evidencian cierto grado de diferenciación entre ellas, probablemente fruto de la antigüedad del establecimiento y de la fragmentación que las poblaciones han sufrido. No existe una relación genético/morfométrico-geográfica ni genético/morfométrico-hidrográfica clara. Puede estar en relación con los movimientos de cangrejos de unos lugares a otros efectuados por el hombre con el fin de perpetuar las poblaciones de cangrejo, o con las fragmentaciones, que han propiciado pequeños tamaños efectivos de las poblaciones y endogamia, posiblemente como consecuencia de los múltiples cuellos de botella donde la deriva genética ha podido jugar un papel muy importante. Estos cuellos de botella que han atravesado las poblaciones podrían deberse principalmente a las diferentes causas que han conducido a la regresión del cangrejo en nuestro país, entre las que se encuentran las alteraciones producidas por el hombre sobre el hábitat, la afanomicosis y la introducción de especies exóticas de cangrejos.La información obtenida puede resultar eficaz para las Administraciones a la hora de diseñar programas de recuperación de las poblaciones de cangrejo autóctono.- Project number: 201
Item Fomentando biólogos emprendedores desde la asignatura de Proyectos y Estudios en Biología (cuarto curso del Grado de Biología)(2022) Morcillo Alonso, Felipe; Beroiz Remírez, Beatriz; Pedro Ormeño, Nuria de; Alfaro Sánchez, David; Jiménez Escobar, María Dolores; López Torres, Mónica; Ortega Moreno, Álvaro Dario; Rello Varona, Santiago; López González-Nieto, Pilar; Urrialde de Andrés, Rafael; Reviriego Eiros, Mario; Pintos López, Beatriz; Roda Ghisleri, Lucía Vera; Sánchez Ballesta, María Teresa; Fernández Messina, Lola María; Trujillo de la Cruz, Marta; Franco López, Daniel; Baratas Díaz, AlfredoEste proyecto se elabora con el objetivo principal de fomentar el emprendimiento en los estudiantes de 4º Grado de Biología para que constituyan su empresa, redacten sus proyectos y participen en la Convocatoria de Premios de Emprendimiento Universitario UCM, en la Convocatoria Preincubadora BIO y, potencialmente, en futuras licitaciones públicas. Los miembros del equipo que han participado en el proyecto están o han estado involucrados a lo largo de varios cursos en la docencia de la asignatura de “Proyectos y Estudios en Biología”. El equipo tiene un marcado carácter interdisciplinar ya que está compuesto por profesores que pertenecen a diferentes unidades docentes de todos los departamentos de la Facultad de Ciencias Biológicas, además de dos estudiantes UCM del Grado de Biología y del Máster de Ecología, egresados del curso académico anterior. La metodología seguida para la consecución de los objetivos del proyecto se ha basado en la búsqueda, la obtención, el análisis de documentos necesarios y, finalmente, la elaboración de los materiales didácticos. Los documentos ofrecidos a los estudiantes han sido consultados en torno al 60-70% por ellos en más de 5 ocasiones. La parte que más les ha ayudado, en todos los casos, es la de la estructura de los documentos, y coinciden, en todos los documentos, en la dificultad de comprensión del lenguaje empleado. Aun así, las actividades realizadas durante el desarrollo de este proyecto han permitido que se alcance el objetivo planteado, siendo conscientes de la necesidad de continuar evaluando los resultados del proyecto a largo plazo. Item Allergy-causing mite identification based on PCR amplification of their ribosomal DNA (Abstract)(2011) Beroiz Remírez, Beatriz; Lombardero, Manuel; Couso-Ferrer, Francisco; Ortego, Félix; Castañera, Pedro; Barber, DomingoItem Mite species identification in the production of allergenic extracts for clinical use and in environmental samples by ribosomal DNA amplification(Medical and Veterinary Entomology, 2014) Beroiz Remírez, Beatriz; Couso-Ferrer, Francisco; Ortego, Félix; Chamorro, María José; Arteaga, Carmen; Lombardero, Manuel; Castañera, Pedro; Hernández-Crespo, PedroThe identification of allergy-causing mites is conventionally based on morphological characters. However, molecular taxonomy using ribosomal DNA (rDNA) may be particularly useful in the analysis of mite cultures and purified mite fractions in the production of allergenic extracts. Full-length internal transcribed spacers (ITS1 and ITS2) were obtained from Dermatophagoides farinae, Dermatophagoides pteronyssinus, Dermatophagoides microceras and Euroglyphus maynei (Astigmata: Pyroglyphidae), Glycyphagus domesticus and Lepidoglyphus destructor (Astigmata: Glycyphagidae), Tyrophagus fanetzhangorum, Tyrophagus putrescentiae, Tyrophagus longior, Tyrophagus neiswanderi, Acarus farris and Acarus siro (Astigmata: Acaridae), and Blomia tropicalis (Astigmata: Echymopodidae), using mite-specific primers. Polymerase chain reaction (PCR) products were digested with HpaII and RsaI restriction enzymes in order to produce species-specific PCR restricted fragment length polymorphism (RFLP) profiles. A semi-nested re-amplification step was introduced before the RFLP in order to apply the method to environmental samples. Results demonstrate that rDNA sequences can be used for the unambiguous identification of mite species. The PCR–RFLP system allows the identification of species in purified mite fractions when the availability of intact adult mite bodies for morphological identification is limited. This reliable and straightforward PCR–RFLP system and the rDNA sequences obtained can be of use in the identification of allergy-causing mite species.Item Mitochondrial DNA variability in Spanish populations of A. italicus inferred from the analysis of a COI region(Knowledge and Management of Aquatic Ecosystems, 2011) Matallanas, Beatriz; Ochando González, María Dolores; Vivero, Almudena; Beroiz Remírez, Beatriz; Alonso, Fernando; Callejas, Carmen; Callejas Hervás, CarmenAustropotamobius italicus was once widely distributed throughout most of the country’s limestone basins in Spain. But its populations have shown a very strong decline over the last thirty years, due to different factors. Thus, the species now enjoys protection under regional, national and international legislation. Therefore, knowledge of the levels and patterns of distribution of genetic diversity in crayfish populations is critical when making conservation management decisions. In the present work, the current genetic structure of Spanish populations of white-clawed crayfish, A. italicus, was analyzed. Eleven Spanish populations and an Italian sample were studied through an 1184 bp-lentgh sequence of cytochrome oxidase subunit I mitochondrial gene. Data analysis revealed the existence of eight haplotypes in the Iberian Peninsula, the highest diversity reported to date in Spanish crayfish. Also a substantial genetic differentiation among populations was found, with a clear geographic pattern. The genetic variability found in these populations is similar to, and even higher, than that reported in previous studies on other Spanish and European populations of A. italicus. Thus, given the current risk status of the species across its range, this variability in certain populations offers some hope for the species from a management point of view.Item Genetic structure of Sesamia nonagrioides (Lefebvre) populations in the mediterranean area(Environmental Entomology, 2008) Beroiz Remírez, Beatriz; Poza, Marta De la; Pérez Farinós, Gema; Ortego, FélixThe Mediterranean corn borer, Sesamia nonagrioides (Lefebvre) (Lepidoptera: Noctuidae), is a major pest of maize in the Mediterranean area. Transgenic Bt maize expressing the Cry1Ab toxin from the bacterium Bacillus thuringiensis can effectively control this pest. The characterization of S. nonagrioides population structure, at a large geographical scale, would provide some insight in decision making for resistance management. The genetic relationships among nine populations from Spain, one from France, one from Italy, three from Greece, and one from Turkey were assessed using Random Amplyfied Polymorphic DNA (RAPD) markers. Populations from France and Spain formed a cluster independent from a cluster of populations collected in Italy, Turkey, and Greece in a unweighted pair-group method with arithmetic average dendrogram constructed from Nei’s genetic distances. Average genetic differentiation among samples was significant for all geographical groupings analyzed (FST = 0.160 ± 0.014 for Spanish populations; 0.133 ± 0.022 for Spanish and French populations; and 0.095 ± 0.010 for Greek, Italian, and Turkish populations). Genetic differentiation was also significant for all paired comparisons of populations, including two Spanish populations separated by only 15 km with no apparent geographical barriers. No pattern of isolation by distance was observed among Mediterranean corn borer populations collected in Spain and France. These results suggest a limited genetic exchange between relatively distant S. nonagrioides populations in Europe, which might contribute to decreased rate of spread of resistance alleles once resistance has developed at a certain site.Item Genetic structure of Spanish white-clawed crayfish (Austropotamobius pallipes) populations as determined by RAPD analysis: reasons for optimism(Aquatic conservation: marine and freshwater ecosystems, 2008) Beroiz Remírez, Beatriz; Callejas Hervás, Carmen; Alonso, Fernando; Ochando González, María Dolores1. Spanish populations of the white-clawed crayfish have declined sharply over the last three decades. Although Austropotamobius pallipes was once widely distributed and very abundant in most of the limestone basins of the country, outbreaks of crayfish plague since 1978 have reduced its populations, and now only some 500–600 small populations are left. 2. Consequently, the species now enjoys protection under national legislation. Management decisions regarding the conservation of a threatened species require an understanding of the genetic structure of its populations. 3. Using random amplified polymorphic DNA (RAPD) fingerprinting the genetic variability of 11 populations of A. pallipes was assessed over the species' range in Spain, and their phylogenetic relationships determined. 4. Substantial genetic differentiation was detected among the populations tested; no clear relationship was found between patterns of genetic variability and hydrological basin. The RAPD markers showed the degree of genetic variability of these populations to be similar to, and in some cases slightly higher than, that reported in previous studies on other Spanish and European populations of A. pallipes. 5. The results offer hope for the recovery of this species in Spain, and provide information that might be useful in the management of crayfish reintroduction programmes.Item Population structure of Banana Weevil, an introduced pest in the Canary Islands, studied by RAPD analysis(Bulletin of Entomological Research, 2007) Magaña, Cristina; Beroiz Remírez, Beatriz; Hernández Crespo, Pedro; Montes de Oca, M.; Carnero, A.; Ortego, Félix; Castañera, PedroThe banana weevil (BW), Cosmopolites sordidus (Coleoptera: Curculionidae), is one of the most important insect pests of bananas and plantains. The mobility and the origin of BW infestations at the Canary Islands (Tenerife, La Gomera and La Palma) have been analysed using Random Amplified Polymorphic DNA (RAPD) as molecular markers. Populations from Costa Rica, Colombia, Uganda and Madeira were also included for comparison. One hundred and fifteen reproducible bands from eight primers were obtained. The level of polymorphism in the populations from the Canary Islands (40–62%) was in the range of those found in other populations. Nei's genetic distances, pair-wise fixation index (FST) values indicate that the closest populations are Tenerife populations among themselves (Nei's genetic distance=0.054–0.100; FST=0.091–0.157) and Costa Rica and Colombia populations (Nei's genetic distance=0.049; FST=0.113). Our results indicate the existence of BW local biotypes with limited gene flow and affected by genetic drift. These results are compatible with a unique event of colonization at Tenerife; whereas, the outbreaks in La Gomera and La Palma may come from independent introductions. The Madeira population is phylogenetically and geographically closer to the Canary Islands populations, suggesting that it is the most likely source of the insects introduced in the Canary Islands.Item Preserving the biodiversity of freshwater ecosystems in a scenario of increasing desertification: lessons from genetics(Handbook of Environmental Research, 2010) Beroiz Remírez, Beatriz; Callejas Hervás, Carmen; Ochando González, María Dolores; Alonso, Fernando; Vivero, Almudena; Matallanas, Beatriz; Edelstein, Aurel & Bär, DagmarThe European Union is faced with the challenge of environmental alteration. In recent years, human activities and accelerating climate change have had a great impact in many regions, with the freshwater ecosystems of Mediterranean countries being hit particularly hard. Spain has the greatest biodiversity of Europe. The country’s maritime barriers, the Pyrenees, and its orographic and climatic peculiarities, invest it with its unique biogeographic characteristics, which together have led to the appearance of many endemic freshwater species. However, the freshwater ecosystems of Spain are suffering great modification at the hands of climate change (some Mediterranean regions now receive 20% less rain than a century ago), environmental degradation, habitat fragmentation, the rise in human demand for water, and a range of human activities; together these factors have contributed to a notable increase in the size of Spain’s arid and semiarid regions, and to changes in its biodiversity. The country’s aquatic biodiversity is now threatened, and the Spanish Ministry of the Environment and the Rural and Marine Environments and the Biodiversity Foundation have seen the need to launch several biodiversity conservation programmes. A major goal of such programmes should be to preserve genetic variability. Indeed, knowledge of the levels and patterns of distribution of genetic diversity in populations of freshwater species is critical when making conservation management decisions. Our group is conducting research into the genetic variation, and its distribution, in two cornerstone freshwater groups: the genus Barbus, which has the greatest species diversity of all Iberian fish genera, and the white-clawed crayfish Austropotamobius pallipes. The latter was once widely distributed throughout most of the country’s limestone basins; now much more rare, it enjoys protection under regional, national and European legislation. The ecological importance and the conservation interest surrounding the above organisms led us to analyse the genetic variability of their populations over their entire distribution ranges. To obtain as complete a picture as possible, nuclear (RAPD, ISSR) and mitochondrial (coI, cyt b and 16S genes) molecular markers were studied. Evidence is provided suggesting that the genetic variability in barbel species is low. The genetic variation seen for A. Pallipes, however, was slightly higher than expected, allowing some hope of its recovery. We are also generating a genetic database for both groups; this could serve as an aid to managers and policy-makers involved in freshwater conservation.