Person:
Mayol Hornero, Beatriz

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First Name
Beatriz
Last Name
Mayol Hornero
URI
https://hdl.handle.net/20.500.14352/85740
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
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2021 - 20224
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Now showing 1 - 4 of 4
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    A glutathione disulfide-sensitive Janus nanomachine controlled by an enzymatic and logic gate for smart delivery
    (Nanoscale, 2021) Mayol Hornero, Beatriz; Díez-Sánchez, Paula; Sánchez Sánchez, Alfredo; Torre, Cristina de la; Villalonga, Anabel; Lucena-Sánchez, Elena; Sancenón, Félix; Martínez Ruiz, María Paloma; Vilela García, Diana; Martínez-Máñez, Ramón; Villalonga Santana, Reynaldo
    This work describes the assembly of a novel enzyme-controlled nanomachine operated through an AND Boolean logic gate for on-command delivery. The nanodevice was constructed on Au-mesoporous silica Janus nanoparticles capped with a thiol-sensitive gate-like molecular ensemble on the mesoporous face and functionalized with glutathione reductase on the gold face. This autonomous nanomachine employed NADPH and glutathione disulfide as input chemical signals, leading to the enzymatic production of reduced glutathione that causes the disruption of the gating mechanism on the mesoporous face and the consequent payload release as an output signal. The nanodevice was successfully used for the autonomous release of doxorubicin in HeLa cancer cells and RAW 264.7 macrophage cells.
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    Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor
    (Biosensors, 2022) Villalonga Chico, Anabel; Parrado Quintela, Concepción; Diaz, Raúl; Sánchez Sánchez, Alfredo; Mayol Hornero, Beatriz; Martínez-Ruíz, Paloma; Vilela García, Diana; Villalonga Santana, Reynaldo
    Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host–guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon electrodes modified with electrodeposited fern-like gold nanoparticles/graphene oxide and, by using a horseradish peroxidase-CD neoglycoenzyme as a biocatalytic redox label. This aptasensor was able to detect the biomarker in the concentration range from 10 pg/mL to 1 ng/mL with a high selectivity and a low detection limit of 3.1 pg/mL in human serum samples.
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    Amperometric aptasensor with sandwich-type architecture for troponin I based on carboxyethylsilanetriol-modified graphene oxide coated electrodes
    (Biosensors and Bioelectronics, 2021) Villalonga, Anabel; Estabiel, Itziar; Pérez-Calabuig, Ana ; Mayol Hornero, Beatriz; Parrado Quintela, Concepción; Villalonga Santana, Reynaldo
    A novel amperometric aptasensor for the specific detection of cardiac troponin I (cTnI) was constructed by using screen-printed carbon electrodes coated with a carboxyethylsilanetriol-modified graphene oxide derivative as transduction element. This novel carboxylic acid-enriched nanomaterial allows easy and high load immobilization of the capture aptamer molecules on the electrode surface. The biosensing interface was assembled by covalent attachment of an amino-functionalized DNA aptamer on the carboxylic acid-enriched electrode surface. The sensing approach relies on the specific recognition of cTnI by the aptamer and further assembly of a sandwich-type architecture with a novel aptamer-peroxidase conjugate as signaling element. The aptasensor was employed to detect the cardiac biomarker in the broad range from 1.0 pg/mL to 1.0 μg/mL with a detection limit of 0.6 pg/mL. This electroanalytical device also showed high specificity, reproducibility and stability, and was useful to quantify cTnI in reconstituted human serum samples.
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    An enzyme-controlled mesoporous nanomachine for triple-responsive delivery
    (Journal of Materials Chemistry B, 2022) Mayol Hornero, Beatriz; Dato, Victor; Rodriguez, Manuel; Lucena, Elena; Villalonga, Anabel; Díez, Paula; Jiménez Falcao, Sandra; Sancenón, Félix; Sánchez Sánchez, Alfredo; Vilela García, Diana; Martínez Ruiz, María Paloma; Martínez-Máñez, Ramón; Villalonga Santana, Reynaldo
    The construction of a novel enzyme-controlled nanomachine with multiple release mechanisms for oncommand delivery is described. This nanodevice was assembled by modifying mesoporous silica nanoparticles with 2-(benzo[d]thiazol-2-yl)phenyl 4-aminobenzoate moieties, and further capped with b-cyclodextrin-modified glucose oxidase neoglycoenzyme. The device released the encapsulated payload in the presence of H2O2 and acidic media. The use of glucose as an input chemical signal also triggered cargo release through the enzymatic production of gluconic acid and hydrogen peroxide, and the subsequent disruption of the gating mechanism at the mesoporous surface. The nanodevice was successfully employed for the enzyme-controlled release of doxorubicin in HeLa cancer cells.