Person: Penelo Hidalgo, Silvia
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First Name
Silvia
Last Name
Penelo Hidalgo
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Medicina y Cirugía Animal
Area
Medicina y Cirugía Animal
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
Item In Vitro Activity of Allium cepa Organosulfur Derivatives against Canine Multidrug-Resistant Strains of Staphylococcus spp. and Enterobacteriaceae(Veterinary Sciences, 2024) Maroto Tello, Alba; Ayllón Santiago, Tania; Aguinaga Casañas, María Arántzazu; Ariza, Juan José; Penelo Hidalgo, Silvia; Baños, Alberto; Ortiz Díez, GustavoBackground: The increase of multi-resistant bacteria, especially Staphylococcus spp. and Enterobacteriaceae, constitutes a challenge in veterinary medicine. The rapid growth of resistance is outpacing antibiotic discovery. Innovative strategies are needed, including the use of natural products like Allium species (Allium sativum L. and Allium cepa L.), which have been used empirically for centuries to treat infectious diseases in humans and farm and aquaculture animals due to their antibacterial properties. Methods: This study aimed to evaluate the in vitro activity of two Alliumderived compounds, propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO), against multi-resistant Staphylococcus spp. (n = 30) and Enterobacteriaceae (n = 26) isolated from dogs referred to a veterinary teaching hospital in Madrid. Results and Discussion: The results indicated the in vitro efficacy of PTSO/PTS against the tested bacterial strains, and 56.7% of Staphylococcus pseudintermedius and 53.8% of Enterobacteriaceae showed sensitivity to PTS and PTSO compared with classic antibiotics. In addition, 50% of S. pseudintermedius strains resistant to erythromycin, ibofloxacin, difloxacin and orbifloxacin and 50% of Enterobacteriaceae strains resistant to tetracycline and doxycycline were sensitive to PTS and PTSO. Although studies are needed to verify their efficacy in vivo, the combined use of PTS and PTSO exhibits promise in enhancing bacterial sensitivity against S. pseudintermedius and Enterobacteriaceae infections, providing a first insight into the potential of both compounds in veterinary practice.Item Active surveillance of antimicrobial resistance in companion animals: A pilot study in a Spanish Veterinary Teaching Hospital(Comparative Immunology, Microbiology and Infectious Diseases, 2024) Scarpellini, Raffaele; Leal Vélez de Mendizábal, Laura; Quevedo Caraballo, Sergio; Blanco Cancelo, José Luis; García Sánchez, Marta Eulalia; Pérez Sancho, Marta; Portero Fuentes, Miriam; Penelo Hidalgo, Silvia; Esposito, Erika; Mondo, Elisabetta; Piva, SilviaThe role of small animal veterinary hospitals in the onset and dissemination of antimicrobial-resistant organisms (AMROs) is still not clear, and the implementation of an internal surveillance systems is a cost-effective tool to better understand their impact. The aim of this study was to describe a pilot program of active surveillance in a Spanish Veterinary Teaching Hospital, developed to estimate the detection frequency of AMROs in the commensal flora of patients and in the environment. Surveillance was focused on Methicillin-resistant Staphylococci (MRS), third generation cephalosporins resistant gram-negative bacteria (3GCR-GNB), and carbapenems-resistant gram-negative bacteria (CR-GNB). Oral and perirectal swabs were collected in the same dogs and cats hospitalized > 48 h, at their admission and before their discharge. Out of 50 patients sampled, 24% (12/50) were carriers at admission of at least one of the three investigated AMROs. Twenty-eight percent of patients (14/50) acquired at least one AMRO during the hospital stay. MRS detection frequency at admission was 12% (6/50), while acquisition was 6% (3/50). 3GCR-GNB detection frequency was 14% at admission (7/50) and acquisition 22% (11/50), while CR-GNB detection frequency was 2% at admission (1/50) and acquisition 2% (1/50). Environmental surveillance (98 samples) showed a total detection frequency of 22.4% for MRS (22/98), 2% for 3GCR-GNB and CR-GNB (2/98). Clinical staff’ shoe soles showed high detection frequency for MRS (50%). 3GCR Escherichia coli was the most isolated species in patients (n = 17). The results show how active surveillance can be used as a tool to assess the impact of AMROs in veterinary hospitals to subsequently build up tailored control plans based on specific issues.Item Estudio y caracterización de cepas de parvovirus canino en España(2017) Penelo Hidalgo, Silvia; Santurde Sánchez, Gloria; Simarro Fernández, IsabelEl parvovirus canino tipo 2 (CPV-2) es uno de los principales agentes etiológicos causantes de gastroenteritis severas en perros. Este virus es pequeño (alrededor de 20nm), sin envuelta, con ADN monocatenario. La cápside virica está compuesta principalmente por la proteína VP2, siendo los cambios de aminoácidos en la secuencia de esta proteína los causantes de la variabilidad genética y antigénica, determinando además el rango de hospedador y el tropismo por determinados tejidos. CPV es muy similar al virus de la panleucopenia felina (FLPV), junto con el que se encuentra clasificado dentro del género Parvoviridae. Ambos son idénticos en un 98% de su secuencia, diferenciándose sólo en la secuencia de 2 aminoácidos de la proteína de la cápside viral VP2. Dentro de este género se encuentran también el virus de la enteritis de los visones (MEV), el parvovirus de los mapaches (RPV) y el parvorirus del zorro azul (BFPV). Se ha postulado que CPV puede provenir de un mutante derivado del parvovirus felino o de algún carnívoro salvaje. CPV-2 emergió a finales de la década de los 70 del siglo pasado, produciendo brotes gastroentericos graves. Tras su emergencia, CPV-2 dio lugar a 2 variantes antigénicas, CPV-2a y CPV-2b, que fueron remplazando progresivamente la cepa original. En el año 2000 se detectó en Italia una nueva variante antigénica, CPV-2c. Esta variante se extendió rápidamente por distintos países. Las distintas varianes antigénicas difieren del original CPV-2 en pocos aminoácidos en la proteína VP2 de la cápside. Las diferencias entre CPV-2a, 2b y 2c residen principalmente en el residuo 426 de la cápside viral, en el que se encuentra asparragina, acido aspártico o ácido glutámico respectivamente...Item A spanish survey on the perioperative use of antimicrobials in small animals(Animals, 2023) Otero Balda, Ignacio; Fuertes Recuero, Manuel; Penelo Hidalgo, Silvia; Espinel Rupérez, Jorge; Lapostolle, Benoit; Ayllón Santiago, Tania; Ortiz Díez, GustavoAppropriate use of perioperative antimicrobials can significantly reduce the risk of post-operative infections. However, inappropriate antimicrobial use can result in the creation of multidrug-resistant bacteria, increased costs, host flora disruption, side effects and increased risk of hospital-acquired infections. This survey evaluated the current perioperative use of antimicrobials in small animals by Spanish veterinarians using a web-based questionnaire. Responses were represented using descriptive statistics and a statistical analysis of the association between demographic data and perioperative antimicrobial use was performed. Pre-operative antimicrobials were administered in clean surgery by up to 68.3% of participants, 81.0% in clean-contaminated surgery and 71.3% in dirty surgery, while in the post-operative period, antimicrobials were administered by up to 86.3% of participants in clean surgery, 93.2% in clean-contaminated surgery and 87.5% in dirty surgery. Factors considered “very important” for antimicrobial selection were the degree of wound contamination, patient immunosuppression and use of prosthesis. The most frequently used antimicrobial was beta-lactamase-resistant (or potentiated) penicillin. Post-operative antimicrobial use was associated with participants without specific surgical postgraduate training. This study highlights an overuse of antimicrobials in perioperative procedures in small animal surgery in Spain. Therefore, evidence-based guidelines and further education regarding the correct use of antimicrobial prophylaxis are recommended.Item Postoperative pain in dogs undergoing either laparoscopic or open ovariectomy(The Veterinary Journal, 2024) Fuertes Recuero, Manuel; Álvarez Gómez De Segura, Ignacio; Sánchez López, A.; Suárez Redondo, María; Canfrán Arrabe, Susana; Penelo Hidalgo, Silvia; Fontanillas Pérez, Juan Carlos; Ortiz Díez, GustavoA prospective, quasi-experimental, clinical trial was performed to assess acute postoperative pain in healthy female dogs following elective ovariectomy by either laparoscopy (n=13) or laparotomy (n=14). Pain was assessed by both a veterinarian at the hospital, and by the owner once the patient was discharged. The Spanish version of the short form of the Glasgow Composite Measuring Pain Scale (CMPS-SF) was used. Pain scores were assessed by the veterinarian preoperatively and at 1, 2, 4, and 6 h after extubation, whilst owner-assessed scores were performed preoperatively and at postoperative days 0, 1, 2, 3, 5 and 7. Data were compared with Mann-Whitney-U test. Veterinarian-assessed CMPS-SF scores were different between both groups at all postoperative times but not at baseline, being below 6/24 in all dogs in the laparoscopy group, but equal to or greater than 6/24 in the laparotomy group at 1 h (n=12), and 4 h (n=4) (P<0.001 and P=0.029, respectively). There were also differences in pain scores between both groups at 2 h (P=0.012) and 6 h (P=0.007), being below 6/24 in all of them. However, there were no differences in owner assessments between groups. In conclusion, ovariectomy performed by laparoscopy induced lower pain scores that were below the pain threshold set by the CMPS-SF during the first 6 h postoperatively. After discharge, and up to one week later, ongoing owner-assessed scores suggest no pain was induced with neither of the techniques. Owners were proactive allowing real-time pain assessment to be reported. The development and validation of instruments for acute pain assessment by owners is warranted, as these tools are currently lacking.