Person:
Moreno Martín, Gustavo

First Name

Gustavo

Last Name

Moreno Martín

URI

https://hdl.handle.net/20.500.14352/85743

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Ciencias Químicas

Department

Química Analítica

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Now showing 1 - 10 of 10

Project number: 202
ODS con mucha ciencia: Hacia una enseñanza STEM y más allá
(2022) Gracia Lor, Emma; Blanco Asenjo, Miriam; Espada Bernabé, Elena; Fernández Bautista, Tamara; Gómez Castro, Emilio; Gómez Mejía, Esther; Gómez Gómez, Beatriz; Lorente Arévalo, Álvaro; Moreno Martín, Gustavo; Muñoz Olivas, María Riansares; Muñoz San Martín, Cristina; Oro Carretero, Paloma de; Pérez Corona, María Teresa; Romero Sánchez, Iván; Sacristán Navarro, Iván; Vicente Zurdo, David
Memoria del proyecto de innovación titulado "ODS con mucha ciencia: Hacia una enseñanza STEM y más allá" de la convocatoria 2021-2022. El proyecto se ha centrado en fomentar el interés de los estudiantes de ESO y Bachillerato por la ciencia, proporcionándoles herramientas para que desarrollen competencias STEM a través de charlas de divulgación, talleres experimentales y sesiones de mentoría.
Biotransformation of selenium by lactic acid bacteria: formation of seleno-nanoparticles and seleno-amino acids
(Frontiers in Bioengineering and Biotechnology, 2020) Martínez, Fernando Gabriel; Moreno Martín, Gustavo; Pescuma, Micaela; Madrid Albarrán, María Yolanda; Mozzi, Fernanda
Selenium (Se) is an essential micronutrient for the majority of living organisms, and it has been identified as selenocysteine in the active site of several selenoproteins such as glutathione peroxidase, thioredoxin reductase, and deiodinases. Se deficiency in humans is associated with viral infections, thyroid dysfunction, different types of cancer, and aging. In several European countries as well as in Argentina, Se intake is below the recommended dietary Intake (RDI). Some lactic acid bacteria (LAB) can accumulate and bio-transform selenite (toxic) into Se-nanoparticles (SeNPs) and Se-amino acids (non-toxic). The microbial growth, Se metabolite distribution, and the glutathione reductase (involved in selenite reduction) activity of Se-enriched LAB were studied in this work. The ninety-six assayed strains, belonging to the genera Lactococcus, Weissella, Leuconostoc, Lactobacillus, Enterococcus, and Fructobacillus could grow in the presence of 5 ppm sodium selenite. From the total, eight strains could remove more than 80% of the added Se from the culture medium. These bacteria accumulated intracellularly between 1.2 and 2.5 ppm of the added Se, from which F. tropaeoli CRL 2034 contained the highest intracellular amount. These strains produced only the seleno-amino acid SeCys as observed by LC-ICP-MS and confirmed by LC-ESI-MS/MS. The intracellular SeCys concentrations were between 0.015 and 0.880 ppm; Lb. brevis CRL 2051 (0.873 ppm), Lb. plantarum CRL 2030 (0.867 ppm), and F. tropaeoli CRL 2034 (0.625 ppm) were the strains that showed the highest concentrations. Glutathione reductase activity values were higher when the strains were grown in the presence of Se except for the F. tropaeoli CRL 2034 strain, which showed an opposite behavior. The cellular morphology of the strains was not affected by the presence of Se in the culture medium; interestingly, all the strains were able to form spherical SeNPs as determined by transmission electron microscopy (TEM). Only two Enterococcus strains produced the volatile Se compounds dimethyl-diselenide identified by GC-MS. Our results show that Lb. brevis CRL 2051, Lb. plantarum CRL 2030, and F. tropaeoli CRL 2034 could be used for the development of nutraceuticals or as starter cultures for the bio-enrichment of fermented fruit beverages with SeCys and SeNPs.
Insights into the accumulation and transformation of Ch-SeNPs by Raphanus sativus and Brassica juncea: effect on essential elements uptake
(Science of the Total Environment, 2020) Moreno Martín, Gustavo; Sanz Landaluce, Jon; León González, María Eugenia De; Madrid Albarrán, María Yolanda; Elsevier
Selenium (Se) at very low doses has important functions for humans. Unfortunately, the low levels of Se in soils in various regions of the world have implemented the agronomic biofortification of crops by applying Se-enriched fertilizers (mainly based on selenate). Lately, the use of nanofertilizers is growing in interest as their low size reduces the amount of chemicals and minimizes nutrient losses in comparison with conventional bulk fertilizers. However, the knowledge on their fate and environmental impact is still scarce. This study aims to evaluate the biotransformation of chitosan-modified Se nanoparticles (Ch-SeNPs) as well as their effect on the metabolism of essential metals (Fe, Cu, Zn and Mo) when applied to hydroponic cultivation of R. sativus and B. juncea. In house-synthesized Ch-SeNPs were characterized in both synthesis and hydroponic culture media by transmission electron microscopy (TEM), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The composition of one-tenth strength Hoagland's solution did not affect the size, shape and concentration in number of particles per mL of Ch-SeNPs. The plants were grown inside a box at 25 °C during the months of May–July in 2018. After a week of treatment with Ch-SeNPs, plants were harvested and divided into roots and aerial part. The biotransformation of Ch-SeNPs was evaluated through a process of enzymatic hydrolysis and subsequent analysis by HPLC-ICP-MS and HPLC-ESI-MS/MS. The results confirmed the transformation of Ch-SeNPs to seleno-amino acids: Selenomethionine (SeMet), Semethylselenocysteine (SeMetSeCys) and ɣ-glutamyl-Se-MetSeCys. Moreover, Multiple-way analysis of variance (ANOVA) and principal component analysis (PCA) showed that, regardless the plant species, Ch-SeNPs supplementation affected the absorption of Zn.
Characterization of AgNPs and AuNPs in sewage sludge by single particle inductively coupled plasma-mass spectrometry
(Talanta, 2022) Moreno Martín, Gustavo; Gómez Gómez, Beatriz; León González, María Eugenia De; Madrid Albarrán, María Yolanda; Elsevier
This study develops for the first time an analytical method for the characterization of silver and gold nanoparticles in sewage sludge. The evaluation of the effect of temperature, extracting agent and centrifugation speed and time on the extraction yield was carried out through a multifactorial analysis of variance which allows us to select 289 g, 5 min and 20 mM sodium pyrophosphate tetrabasic as optimal extraction conditions. Under these conditions, the analysis of the extract by single particle inductively coupled plasma-mass spectrometry provided recovery percentages of 70 ± 2% and 56 ± 1% for silver and gold nanoparticles, respectively. Moreover, the complementary results obtained upon analysis of these extracts by transmission electron microscopy and single particle inductively coupled plasma-mass spectrometry showed that the developed method did not modify the original size and shape of these nanoparticles during the extraction procedure. Size detection limits of 23 nm and 16 nm as well as number concentration limits of 3.12 × 109 particles kg−1 and 1.38 × 109 particles kg−1 were obtained for silver and gold nanoparticles, respectively. Moreover, a stability study of silver and gold nanoparticles in sewage sludge for 12 months showed differences between the two nanoparticle types. Although the sizes were not affected during the 12 months, silver nanoparticles underwent an oxidation process from 6 months onwards, which was reflected in an increase in the percentage of ionic silver from 14 ± 1% at 6 months to 24 ± 2% at 12 months. The developed methodology represents a simple, reliable and fast tool for detecting, quantifying and assessing the stability of nanoparticles in an important environmental sample such as sewage sludge.
Caracterización y transformación de nanopartículas metálicas y de metaloides en muestras biológicas y medioambientales
(2022) Moreno Martín, Gustavo; Madrid Albarrán, Yolanda; León González, María Eugenia de
La nanotecnología, y en particular las nanopartículas metálicas y de metaloides han demostrado ser de gran utilidad en aplicaciones relacionadas con la salud, el medioambiente, la tecnología o la alimentación. Sin embargo, su liberación al medioambiente a lo largo de su ciclo de utilización puede constituir un riesgo potencial y poco conocido para la salud, el medioambiente y los ecosistemas. Las nanopartículas una vez que llegan al medioambiente e interaccionan con las sustancias químicas del entorno o con sistemas biológicos pueden experimentar modificaciones en sus propiedades fisicoquímicas (tamaño, morfología, estado de agregación, carga superficial, procesos de disolución o transformación a otras especies químicas del mismo elemento). Todo ello afectará sin duda a su destino final e impacto sobre los ecosistemas. En este sentido, la Química Analítica, juega un papel clave para establecer dichas transformaciones, mediante el desarrollo de nuevas estrategias y/o la adaptación y combinación de técnicas y métodos existentes. La presente Tesis Doctoral titulada “Caracterización y transformación de nanopartículas metálicas y de metaloides en muestras biológicas y medioambientales” aborda esta problemática mediante el desarrollo de metodologías analíticas que permitan caracterizar y estudiar las transformaciones que sufren las nanopartículas metálicas de plata (AgNPs) y de oro (AuNPs) al estar en contacto con muestras medioambientales de distinta naturaleza, y las nanopartículas de selenio (SeNPs) en su interacción con sistemas biológicos como plantas y bacterias...
Aprendizaje-Servicio para trasladar los resultados desarrollo sostenible: del laboratorio a la sociedad
(2021) Gracia Lor, Emma; Gómez Mejía, Esther; Moreno Martín, Gustavo; Gómez Gómez, Beatriz; Vicente Zurdo, David; Pérez Corona, María Teresa; Muñoz Olivas, María Riansares; Gómez Castro, Emilio
El objetivo general planteado en este proyecto ha sido comunicar y concienciar a los estudiantes de secundaria y bachillerato sobre la necesidad de un desarrollo sostenible, con el fin de que sean capaces de comprender e identificar las problemáticas asociadas a los objetivos planteados en la Agenda 2030 y asociarlas con actividades propias de la vida cotidiana. De esta manera, los alumnos podrán adquirir herramientas de gran utilidad para el desarrollo de su futura vida personal y profesional. Para lograr dichos objetivos se han llevado a cabo una serie de actividades (charlas de divulgación científica, debates, uso de la aplicación Kahoot) en diferentes centros educativos no universitarios, en concreto, en tres institutos de educación secundaria y bachillerato (IES) y en un centro de educación de personas adultas (CEPA). Los temas tratados se encuadran dentro de los Objetivos de Desarrollo Sostenible (ODS) de la Agenda 2030, concretamente los ODS 2, 3 y 12.
Selenium stress response of the fruit origin strain Fructobacillus tropaeoli CRL 2034
(Applied Microbiology and Biotechnology, 2023) Martínez, Fernando Gabriel; Moreno Martín, Gustavo; Mozzi, Fernanda; Madrid Albarrán, María Yolanda; Pescuma, Micaela
The fruit-origin strain Fructobacillus tropaeoli CRL 2034 can biotransform selenium into seleno-nanoparticles and selenocysteine. The proteomic analysis of F. tropaeoli CRL 2034 exposed to 5 and 100 ppm of Se showed a dose-dependent response since 19 and 77 proteins were deregulated, respectively. In the presence of 5 ppm of Se, the deregulated proteins mainly belonged to the categories of energy production and conversion or had unknown functions, while when cells were grown with 100 ppm of Se, most of the proteins were grouped into amino acid transport and metabolism, nucleotide transport and metabolism, or into unknown functions. However, under both Se conditions, glutathione reductases were overexpressed (1.8–3.1-fold), while mannitol 2-dehydrogenase was downregulated (0.54–0.19-fold), both enzymes related to oxidative stress functions. Mannitol 2-dehydrogenase was the only enzyme found that contained SeCys, and its activity was 1.27-fold increased after 5 ppm of Se exposure. Our results suggest that F. tropaeoli CRL 2034 counteracts Se stress by overexpressing proteins related to oxidative stress resistance and changing the membrane hydrophobicity, which may improve its survival under (food) storage and positively influence its adhesion to intestinal cells. Selenized cells of F. tropaeoli CRL 2034 could be used for producing Se-enriched fermented foods. Graphical Abstract: [Figure not available: see fulltext.] Key points: • Selenized cells of F. tropaeoli showed enhanced resistance to oxidative stress. • SeCys was found in the Fructobacillus mannitol 2-dehydrogenase polypeptide chain. • F. tropaeoli mannitol 2-dehydrogenase activity was highest when exposed to selenium.
Project number: 283
La divulgación científica como herramienta para promover el desarrollo sostenible: de la universidad a la sociedad
(2021) Gracia Lor, Emma; Muñoz Olivas, María Riansares; Pérez Corona, María Teresa; Gómez Castro, Emilio; Gómez Mejía, Esther; Moreno Martín, Gustavo; Vicente Zurdo, David; Gómez Gómez, Beatriz
Memoria del proyecto de innovación titulado "La divulgación científica como herramienta para promover el desarrollo sostenible: de la universidad a la sociedad" de la convocatoria 2020-2021. El proyecto se ha centrado en promover una educación de calidad mediante la transferencia de conocimientos científicos relacionados con los Objetivos de Desarrollo Sostenible 2, 3 y 12 de la Agenda 2030 y el desarrollo de pensamiento crítico científico-divulgativo tanto del alumnado procedente de centros educativos de secundaria y bachillerato como de los estudiantes de universidad.
Evaluation of the transformation of selenite and selenium nanoparticles to seleno-amino acids produced by Escherichia coli and Staphylococcus aureus by using liquid chromatography -inductively coupled plasma mass spectrometry and single-particle- inductively coupled plasma mass spectrometry and different sample treatments
(Spectrochimica Acta Part B: Atomic Spectroscopy, 2023) Moreno Martín, Gustavo; Espada-Bernabé, Elena; Gómez Gómez, Beatriz; León González, María Eugenia De; Madrid Albarrán, María Yolanda
Due to the scarce knowledge about the impact of selenium nanoparticles (SeNPs) on bacterial populations, the main objective of this work was focused on evaluating the transformations of SeNPs and selenite in Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). For this purpose, an analytical methodology based on bacteria cell wall disruption, carbamidomethylation, enzymatic hydrolysis and high-performance liquid chromatography couple to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) and HPLC- electrospray tandem mass spectrometry (HPLC-ESI-MS/MS) measurements was developed, the latter for unambiguous identification of selenium species. Once bacteria were cultured in the presence of chitosan modified SeNPs (Ch-SeNPs) and selenite at 0, 1 and 2 mg L􀀀 1 Se for 24 h, an enzymatic disruption of the bacterial cell wall using lysozym followed by enzymatic hydrolysis with protease was applied. The use of lysozyme to extract selenium speciesprovided a better efficiency in the total selenium content (higher than 96%), compared to a mechanical disruption of the bacterial cell wall. Analysis of the extracts by anionic exchange HPLC-ICP-MS showed a strong influence of incubation time with protease (24, 48 and 72 h) on selenium chromatographic profile. The results showed that selenocysteine (SeCys) was the only Se species identified in both bacteria representing an 80% of total selenium accumulated. The confirmation of the identity of this Se species was carried out after performing a carbamidomethylation process, prior to enzymatic hydrolysis, and analyzing the extract by reversed phase HPLCESI- MS/MS. These analyses confirmed the presence of SeCys, and no relevant differences were found between the metabolic pathway of both forms of selenium. Moreover, the growth of both bacterial species in the presence of selenite resulted in the formation of biogenic SeNPs. Characterization by TEM before and after their separation from the culture medium showed spherical and monodispersed nanoparticles with an average size (155 ± 19) nm and (172 ± 20) nm for E. coli and S. aureus, respectively. Analysis by spICP-MS showed no significant differences in size with respect to TEM after considering the ionization efficiency of Se.
In vivo quantification of volatile organoselenium compounds released by bacteria exposed to selenium with HS-SPME-GC-MS. Effect of selenite and selenium nanoparticles
(Talanta, 2021) Moreno Martín, Gustavo; Sanz Landaluce, Jon; León González, María Eugenia De; Madrid Albarrán, María Yolanda; Elsevier
Quantification of volatile organoselenium species released by Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), after their growth in the presence of 1 and 2 mg Se⋅L-1 as both selenite and chitosan modified selenium nanoparticles (Ch-SeNPs), was achieved by the application of a method based on headspace solid-phase microextraction (HS-SPME) and in-fiber internal standardization, combined with gas chromatography coupled to mass spectrometry (GC-MS). This method consisted of an initial extraction of the released volatile organoselenium compounds on the SPME fiber, followed by the extraction of internal standard (IS), deuterated dimethyl sulfide (d6-DMS), on the same fiber before its desorption at the injection port of GC-MS. The results showed that the biotransformation of selenite and Ch-SeNPs into volatile organoselenium compounds was dependent on both the type of bacterial species and the chemical form of selenium (Se) administered. In this sense, E. coli was able to biotransform both selenite and Ch-SeNPs into dimethylselenium (DMSe) and dimethyldiselenium (DMDSe) while S. aureus, biotransformed selenite into DMSe and DMDSe and, Ch-SeNPs only into DMDSe. Additionally, the formation of a volatile mixed sulfur/selenium compound, dimethyl selenenyl sulfide (DMSeS), from Se in nanoparticulated form has been detected for the first time.