Person: Rubio Retama, Benito Jorge
Loading...
First Name
Benito Jorge
Last Name
Rubio Retama
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
QuÃmica en Ciencias Farmacéuticas
Area
QuÃmica FÃsica
Identifiers
13 results
Search Results
Now showing 1 - 10 of 13
Item Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection(ACS Applied Materials & Interfaces, 2016) Laurenti, Marco; Paez-Pérez, Miguel; Algarra González, Manuel; Alonso Cristobal, Paulino; López Cabarcos, Enrique; Méndez González, Diego; Rubio Retama, Benito JorgeWe developed a sensor for the detection of specific microRNA (miRNA) sequences that was based on graphene quantum dots (GQDs) and ssDNA-UCNP@SiO2. The proposed sensor exploits the interaction between the sp2 carbon atoms of the GQD, mainly π–π stacking, and the DNA nucleobases anchored on the upconversion nanoparticles (UCNPs). This interaction brings the GQD to the surface of the ssDNA-UCNP@SiO2 system, enhancing the upconversion emission. On the other hand, hybridization of the single-stranded DNA (ssDNA) chains anchored on the nanoparticles with their complementary miRNA sequences blocks the capacity of the UCNPs to interact with the GQD through π–π stacking. That gives as result a reduction of the fluorescent enhancement, which is dependent on the concentration of miRNA sequences. This effect was used to create a sensor for miRNA sequences with a detection limit of 10 fM.Item Exploring the Origin of the Thermal Sensitivity of Near-Infrared-II Emitting Rare Earth Nanoparticles(Applied Materials and Interfaces, 2023) Hamraoui, Khouloud; Torres Vera, Vivian Andrea; Zabala Gutiérrez, Irene; Casillas Rubio, Alejandro; Alqudwa Fattouh, Mohammed; Benayas, Antonio; MarÃn, Riccardo; Natile, Marta MarÃa; Manso Silván, Miguel; Rubio Zuazo, Juan; Jaque, Daniel; Melle Hernández, Sonia; Gómez Calderón, Óscar; Rubio Retama, Benito JorgeRare-earth doped nanoparticles (RENPs) are attracting increasing interest in materials science due to their optical, magnetic, and chemical properties. RENPs can emit and absorb radiation in the second biological window (NIR-II, 1000-1400 nm) making them ideal optical probes for photoluminescence (PL) in vivo imaging. Their narrow emission bands and long PL lifetimes enable autofluorescence-free multiplexed imaging. Furthermore, the strong temperature dependence of the PL properties of some of these RENPs makes remote thermal imaging possible. This is the case of neodymium and ytterbium co-doped NPs that have been used as thermal reporters for in vivo diagnosis of, for instance, inflammatory processes. However, the lack of knowledge about how the chemical composition and architecture of these NPs influence their thermal sensitivity impedes further optimization. To shed light on this, we have systematically studied their emission intensity, PL decay time curves, absolute PL quantum yield, and thermal sensitivity as a function of the core chemical composition and size, active-shell, and outer-inert-shell thicknesses. The results revealed the crucial contribution of each of these factors in optimizing the NP thermal sensitivity. An optimal active shell thickness of around 2 nm and an outer inert shell of 3.5 nm maximize the PL lifetime and the thermal response of the NPs due to the competition between the temperature-dependent back energy transfer, the surface quenching effects, and the confinement of active ions in a thin layer. These findings pave the way for a rational design of RENPs with optimal thermal sensitivity.Item Boosting the Near-Infrared Emission of Ag2S Nanoparticles by a Controllable Surface Treatment for Bioimaging Applications(ACS Applied Materials & Interfaces, 2022) Zabala Gutiérrez, Irene; Gerke, Christoph; Shen, Yingli; Ximendes, Erving Clayton; Manso Silvan, Miguel; Marin, Riccardo; Jaque GarcÃa, Daniel; Gómez Calderón, Óscar; Melle Hernández, Sonia; Rubio Retama, Benito JorgeAg2S nanoparticles are the staple for high-resolution preclinical imaging and sensing owing to their photochemical stability, low toxicity, and photoluminescence (PL) in the second near-infrared biological window. Unfortunately, Ag2S nanoparticles exhibit a low PL efficiency attributed to their defective surface chemistry, which curbs their translation into the clinics. To address this shortcoming, we present a simple methodology that allows to improve the PL quantum yield from 2 to 10%, which is accompanied by a PL lifetime lengthening from 0.7 to 3.8 μs. Elemental mapping and X-ray photoelectron spectroscopy indicate that the PL enhancement is related to the partial removal of sulfur atoms from the nanoparticle’s surface, reducing surface traps responsible for nonradiative de-excitation processes. This interpretation is further backed by theoretical modeling. The acquired knowledge about the nanoparticles’ surface chemistry is used to optimize the procedure to transfer the nanoparticles into aqueous media, obtaining water-dispersible Ag2S nanoparticles that maintain excellent PL properties. Finally, we compare the performance of these nanoparticles with other near-infrared luminescent probes in a set of in vitro and in vivo experiments, which demonstrates not only their cytocompatibility but also their superb optical properties when they are used in vivo, affording higher resolution images.Item 10-Fold Quantum Yield Improvement of Ag2S Nanoparticles by Fine Compositional Tuning(ACS Applied materials and interfaces, 2020) Ortega RodrÃguez, Alicia; Shen, Yingli; Zabala Gutiérrez, Irene; Santos, Harrison D. A.; Torres Vera, Vivian Andrea; Ximedes, Erving; Villaverde Cantizano, Gonzalo; Lifante, José; Gerke, Christoph; Fernández Monsalve, Nuria; Gómez Calderón, Óscar; Melle Hernández, Sonia; Marqués Hueso, José; Méndez González, Diego; Laurenti, Marco; Jones, Callum M. S.; López Romero, Juan Manuel; Contreras Cáceres, Rafael; Jaque GarcÃa, Daniel; Rubio Retama, Benito Jorge; Garma Pons, SantiagoAg2S semiconductor nanoparticles (NPs) are near-infrared luminescent probes with outstanding properties (good biocompatibility, optimum spectral operation range, and easy biofunctionalization) that make them ideal probes for in vivo imaging. Ag2S NPs have, indeed, made possible amazing challenges including in vivo brain imaging and advanced diagnosis of the cardiovascular system. Despite the continuous redesign of synthesis routes, the emission quantum yield (QY) of Ag2S NPs is typically below 0.2%. This leads to a low luminescent brightness that avoids their translation into the clinics. In this work, an innovative synthetic methodology that permits a 10-fold increment in the absolute QY from 0.2 up to 2.3% is presented. Such an increment in the QY is accompanied by an enlargement of photoluminescence lifetimes from 184 to 1200 ns. The optimized synthetic route presented here is based on a fine control over both the Ag core and the Ag/S ratio within the NPs. Such control reduces the density of structural defects and decreases the nonradiative pathways. In addition, we demonstrate that the superior performance of the Ag2S NPs allows for high-contrast in vivo bioimaging. .Item A brighter era for silver chalcogenide semiconductor nanocrystals(Optical Materials, 2023) Ming, Liyan; Zabala Gutiérrez, Irene; Gómez Calderón, Óscar; Melle Hernández, Sonia; Ximendes , Erwing; Rubio Retama, Benito Jorge; MarÃn, RiccardoSilver chalcogenide semiconductor nanocrystals (Ag2E SNCs) have become a household name in the biomedical field, where they are used as contrast agents in bioimaging, photothermal therapy agents, and luminescent nanothermometers. The prominent position they have come to occupy in this field stems from a unique combination of features, above all near-infrared excitation and emission alongside low cytotoxicity. However, the first reports on Ag2E SNCs showed that a great limitation of these luminescent nanomaterials resided in their low photoluminescence quantum yield, which results in reduced brightness: a crippling feature in bioimaging and biosensing. In this article, we provide an overview of the strategies developed to overcome this hurdle. These strategies aim to remedy the presence of defects in the SNC core and/or surface, the presence of metallic silver, and off-stoichiometric composition. These features stem from the high mobility and redox potential of Ag+ ions, alongside the difficulty in controlling the nucleation and growth rate of Ag2E SNCs. The effectiveness of each approach is discussed. Lastly, a perspective on future research efforts to make Ag2E SNCs even brighter – and thus more effective in biomedical applications – is provided, with the hope of inspiring further investigation on these nanomaterials with a rich, complex set of physicochemical and spectroscopic properties.Item Contribution of resonance energy transfer to the luminescence quenching of upconversion nanoparticles with graphene oxide(Journal of Colloid and Interface Science, 2020) Méndez González, Diego; Gómez Calderón, Óscar; Melle Hernández, Sonia; González Izquierdo, Jesús; Bañares Morcillo, Luis; López DÃaz, David; Velazquez Salicio, M. Mercedes; López Cabarcos, Enrique; Rubio Retama, Benito Jorge; Laurenti, MarcoUpconversion nanoparticles (UCNP) are increasingly used due to their advantages over conventional fluorophores, and their use as resonance energy transfer (RET) donors has permitted their application as biosensors when they are combined with appropriate RET acceptors such as graphene oxide (GO). However, there is a lack of knowledge about the design and influence that GO composition produces over the quenching of these nanoparticles that in turn will define their performance as sensors. In this work, we have analysed the total quenching efficiency, as well as the actual values corresponding to the RET process between UCNPs and GO sheets with three different chemical compositions. Our findings indicate that excitation and emission absorption by GO sheets are the major contributor to the observed luminescence quenching in these systems. This challenges the general assumption that UCNPs luminescence deactivation by GO is caused by RET. Furthermore, RET efficiency has been theoretically calculated by means of a semiclassical model considering the different nonradiative energy transfer rates from each Er3+ ion to the GO thin film. These theoretical results highlight the relevance of the relative positions of the Er3+ ions inside the UCNP with respect to the GO sheet in order to explain the RET-induced efficiency measurements.Item The effects of dopant concentration and excitation intensity on the upconversion and downconversion emission processes of β-NaYF4:Yb3+,Er3+nanoparticles(Journal of materials chemistry C, 2021) Torres Vera, Vivian Andrea; Méndez González, Diego; Ramos Ramos, D. J.; Igalla El-Youssfi, Asmae; Laurenti, Marco; Contreras Cáceres, Rafael; López Cabarcos, Enrique; DÃaz GarcÃa, Elena; Rubio Retama, Benito Jorge; Melle Hernández, Sonia; Gómez Calderón, ÓscarThe dopant concentration of lanthanide ions in photon upconversion nanoparticles (UCNPs) remains one of the key points to boost the brightness of these nanomaterials and, therefore, their application developments. Here, we analyzed the effect of Er3+ and Yb3+ dopant concentrations of b-NaYF4:Yb3+,Er3+ nanoparticles on the visible upconversion and near-infrared downconversion luminescence intensities. Our approach carefully excluded all other factors whose variation affects luminescence properties such as the size, morphology, crystal structure, ion distribution, ligand, and surrounding medium, allowing us to exactly infer the influence of the ratio of Yb3+ to Er3+ ions on the nanoparticle luminescence. To maintain the size and morphological properties of nanoparticles, we used a total dopant concentration of 22% while varying the ratio of Yb3+ to Er3+ ions from 0 to 10. A huge increase in luminescence takes place as the Yb/Er ratio increases following a power-law behavior, and this luminescence enhancement is greater at low excitation intensities. Above a Yb/Er ratio of around two, saturation occurs with a slight peak when this ratio is around four. Simulations using a rate equation model showed that upconversion luminescence (UCL) is mainly produced by the energy transfer between neighboring Er3+ ions at low Yb/Er ratios, while at high ratios, the energy transfer from Yb3+ to Er3+ ions dominates. However, downconversion luminescence (DCL) is produced at all analyzed ratios, except 0, by the previous mechanism.Item SPION nanoparticles for delivery of dopaminergic isoquinoline and benzazepine derivatives(Bioorganic & Medicinal Chemistry, 2022) Lucena-Serrano, Cristina; Lucena-Serrano, Ana; DÃaz, Amelia; Valpuesta, MarÃa; Villaverde Cantizano, Gonzalo; López-Romero, J. Manuel; Sarabia, Francisco; Laurenti, Marco; Rubio Retama, Benito Jorge; Contreras Cáceres, RafaelSuperparamagnetic iron nanoparticles (SPIONs) have become one of the most useful colloidal systems in nanomedicine. We report here the preparation of new hybrid core@shell systems based on SPION nanoparticles coated with a SiO2 shell (SPION@SiO2) and functionalized with carboxyl groups SPION@SiO2-COOH). A series of new N-alkylamino- and N-alkylamido-terminated 1-phenyl- tetrahydroisoquinolines (THIQs) and 3 tetrahydrobenzazepines (THBs) derivatives presenting -SMe and -Cl groups, respectively, with potential dopaminergic activity, are synthesized and incorporated to the hybrid system. We include the synthetic details for THIQs and THBs derivatives preparation and investigate the influence of the terminal-functional group as well as the number of carbon atoms linked to THIQ and THB molecules during the coupling to the SPION@SiO2-COOH. Nuclear magnetic resonance (NMR) and electron ionization mass spectrometry (EI-MS) are used to characterize the synthesized THIQs and THBs. High-angle annular dark-field transmission electron microscopy (HAADFTEM), energy dispersive X-ray transmission electron microscopy (EDX-TEM), and proton high-resolution magic angle spinning NMR spectroscopy1 H HRMAS-NMR) are used to confirm the presence of THB and THIQ molecules onto the surface of the nanoparticles. The hybrid SPION@SiO2-THIQ and THB systems show significant activity toward the D2 receptor, reaching Ki values of about 20 nM, thus having potential application in the treatment of central nervous system (CNS) diseases.- Project number: 74
Item Las TICs: un instrumento de ayuda en las prácticas de laboratorio(2022) Laurenti, Marco; Ovejero Paredes, Karina; Zabala Gutiérrez, Irene; Sanchez Sanche, Visitación; Yusta San Roman, Piedad; Contreras Cáceres, Rafael; Filice, Marco; López Cabarcos, Enrique; Marciello, Marzia; Méndez González, Diego; Rubio Retama, Benito Jorge; Villaverde Cantizano, GonzaloDesde la implantación del Espacio Europeo de Educación Superior nos encontramos ante dos grandes retos; el desarrollo metodológico y tecnológico en la formación y docencia on-line y en segundo lugar la cada vez más elevada especialización requerida a los estudiantes durante el Grado y Doble Grado en Farmacia. Esto hace que el foco de la formación de los estudiantes se desvÃe de los conocimientos básicos y competencias de formación tanto en el trabajo experimental como en el conocimiento teórico. El proyecto se encuadra en el marco de la asignatura obligatoria FÃsica Aplicada a Farmacia de Grado y Doble Grado en Farmacia, unas carreras con una elevada carga práctica y experimental. - Project number: 382
Item Uso de la infografÃa como herramienta didáctica en la enseñanza de las asignaturas de FÃsico QuÃmica Farmacéutica y FÃsica Aplicada a Farmacia: Formación del profesorado.(2023) Aranaz Corral, Inmaculada; Acosta Contreras, Florentina Niuris; Alcántara León, Andrés Rafael; Civera Tejuca, MarÃa Concepción; Heras Caballero, Angeles MarÃa; Izquierdo GarcÃa, José Luis; López Duarte, Ismael; Padilla Mondejar, Sussette; Rodriguez-Ramirez de Arellano, Ignacio; Rubio Retama, Benito Jorge; Villaverde Cantizano, Gonzalo; Alayeto Martinez, Idoia; Fraile Gutierrez, Isabel; Rodriguez Gonzalez, Daniel; Rosa Maceda, Ines de la Rosa; Torres Vera, Vivian; Yin Ying, Susana; Zabala Gutiérrez, Irena; Jimenez Vazquez, Miguel Angel; RodrÃguez Veiga, Isabel