Person:
Rubio Retama, Benito Jorge

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First Name
Benito Jorge
Last Name
Rubio Retama
URI
https://hdl.handle.net/20.500.14352/77471
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Física
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Author: Algarra González, Manuel × Item Type: Publication ×

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    Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection
    (ACS Applied Materials & Interfaces, 2016) Laurenti, Marco; Paez-Pérez, Miguel; Algarra González, Manuel; Alonso Cristobal, Paulino; López Cabarcos, Enrique; Méndez González, Diego; Rubio Retama, Benito Jorge
    We developed a sensor for the detection of specific microRNA (miRNA) sequences that was based on graphene quantum dots (GQDs) and ssDNA-UCNP@SiO2. The proposed sensor exploits the interaction between the sp2 carbon atoms of the GQD, mainly π–π stacking, and the DNA nucleobases anchored on the upconversion nanoparticles (UCNPs). This interaction brings the GQD to the surface of the ssDNA-UCNP@SiO2 system, enhancing the upconversion emission. On the other hand, hybridization of the single-stranded DNA (ssDNA) chains anchored on the nanoparticles with their complementary miRNA sequences blocks the capacity of the UCNPs to interact with the GQD through π–π stacking. That gives as result a reduction of the fluorescent enhancement, which is dependent on the concentration of miRNA sequences. This effect was used to create a sensor for miRNA sequences with a detection limit of 10 fM.