Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection

Laurenti, Marco; Paez-Pérez, Miguel; Algarra González, Manuel; Alonso Cristobal, Paulino; López Cabarcos, Enrique; Méndez González, Diego; Rubio Retama, Benito Jorge

Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection

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Official URL

https://doi.org/10.1021/acsami.6b02361

Publication date

2016

Authors

Laurenti, Marco

Paez-Pérez, Miguel

Algarra González, Manuel

Alonso Cristobal, Paulino

López Cabarcos, Enrique

Méndez González, Diego

Rubio Retama, Benito Jorge

Publisher

American Chemical Society

Citations

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URI

https://hdl.handle.net/20.500.14352/101129

Citation

Laurenti M, Paez-Perez M, Algarra M, Alonso-Cristobal P, Lopez-Cabarcos E, Mendez-Gonzalez D, et al. Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection. ACS Appl Mater Interfaces 2016;8:12644–51. https://doi.org/10.1021/acsami.6b02361.

Abstract

We developed a sensor for the detection of specific microRNA (miRNA) sequences that was based on graphene quantum dots (GQDs) and ssDNA-UCNP@SiO2. The proposed sensor exploits the interaction between the sp2 carbon atoms of the GQD, mainly π–π stacking, and the DNA nucleobases anchored on the upconversion nanoparticles (UCNPs). This interaction brings the GQD to the surface of the ssDNA-UCNP@SiO2 system, enhancing the upconversion emission. On the other hand, hybridization of the single-stranded DNA (ssDNA) chains anchored on the nanoparticles with their complementary miRNA sequences blocks the capacity of the UCNPs to interact with the GQD through π–π stacking. That gives as result a reduction of the fluorescent enhancement, which is dependent on the concentration of miRNA sequences. This effect was used to create a sensor for miRNA sequences with a detection limit of 10 fM.